Home

Download

Statistics

Examples

Help

Contact

Fusion Protein:SLC25A25-TCEB3

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SLC25A25-TCEB3
	FusionPDB ID: 82621
	FusionGDB2.0 ID: 82621
		Hgene	Tgene
	Gene symbol	SLC25A25	TCEB3
	Gene ID	114789	6924
	Gene name	solute carrier family 25 member 25	elongin A
	Synonyms	MCSC\|PCSCL\|SCAMC-2	SIII\|SIII p110\|TCEB3\|TCEB3A
	Cytomap	9q34.11	1p36.11
	Type of gene	protein-coding	protein-coding
	Description	calcium-binding mitochondrial carrier protein SCaMC-2mitochondrial ATP-Mg/Pi carrier protein 3mitochondrial Ca(2+)-dependent solute carrier protein 3short calcium-binding mitochondrial carrier 2small calcium-binding mitochondrial carrier 2solute carr	elongin-ARNA polymerase II transcription factor SIII subunit A1elongin 110 kDa subunittranscription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)transcription elongation factor B alpha subunittranscription elongation factor B polypept
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000373068, ENST00000373069, ENST00000373064, ENST00000373066, ENST00000432073, ENST00000433501,	ENST00000487554, ENST00000418390, ENST00000609199,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	8 X 5 X 6=240	6 X 5 X 3=90
	# samples	8	6
	** MAII score	log2(8/240*10)=-1.58496250072116 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(6/90*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: SLC25A25 [Title/Abstract] AND TCEB3 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: SLC25A25 [Title/Abstract] AND TCEB3 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SLC25A25(130830859)-TCEB3(24075511), # samples:1
Anticipated loss of major functional domain due to fusion event.	SLC25A25-TCEB3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SLC25A25-TCEB3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SLC25A25-TCEB3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SLC25A25-TCEB3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr9:130830859/chr1:24075511)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across SLC25A25 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TCEB3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000373068	SLC25A25	chr9	130830859	-	ENST00000418390	TCEB3	chr1	24075511	+	5110	380	29	2623	864
ENST00000373068	SLC25A25	chr9	130830859	-	ENST00000609199	TCEB3	chr1	24075511	+	2944	380	29	2623	864
ENST00000373069	SLC25A25	chr9	130830859	-	ENST00000418390	TCEB3	chr1	24075511	+	5018	288	27	2531	834
ENST00000373069	SLC25A25	chr9	130830859	-	ENST00000609199	TCEB3	chr1	24075511	+	2852	288	27	2531	834

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000373068	ENST00000418390	SLC25A25	chr9	130830859	-	TCEB3	chr1	24075511	+	0.001509515	0.99849045
ENST00000373068	ENST00000609199	SLC25A25	chr9	130830859	-	TCEB3	chr1	24075511	+	0.00484461	0.99515545
ENST00000373069	ENST00000418390	SLC25A25	chr9	130830859	-	TCEB3	chr1	24075511	+	0.001439741	0.99856025
ENST00000373069	ENST00000609199	SLC25A25	chr9	130830859	-	TCEB3	chr1	24075511	+	0.004579452	0.9954205

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for SLC25A25-TCEB3

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
SLC25A25	chr9	130830859	TCEB3	chr1	24075511	288	85	GLRRLGLHRTEGELQLLKYLKKLSTL
SLC25A25	chr9	130830859	TCEB3	chr1	24075511	380	115	GLRRLGLHRTEGELQLLKYLKKLSTL

Top

Potential FusionNeoAntigen Information of SLC25A25-TCEB3 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

SLC25A25-TCEB3_130830859_24075511.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:03	GELQLLKY	0.9998	0.8646	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:01	GELQLLKY	0.9965	0.6399	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:05	HRTEGELQL	0.9994	0.8207	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:02	HRTEGELQL	0.9994	0.5976	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:04	HRTEGELQL	0.9993	0.7355	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:07	HRTEGELQL	0.9993	0.7112	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:24	HRTEGELQL	0.9983	0.7046	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:06	HRTEGELQL	0.9978	0.8947	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:01	HRTEGELQL	0.9976	0.9257	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:03	GELQLLKYL	0.9968	0.872	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B14:01	HRTEGELQL	0.9962	0.9059	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B14:02	HRTEGELQL	0.9962	0.9059	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B13:01	GELQLLKYL	0.9952	0.6495	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:02	HRTEGELQL	0.991	0.9742	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:01	HRTEGELQL	0.9902	0.9748	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:10	HRTEGELQL	0.8018	0.8167	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B48:01	HRTEGELQL	0.7869	0.8123	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:37	HRTEGELQL	0.7631	0.8275	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:18	HRTEGELQL	0.5981	0.9112	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:13	GELQLLKYL	0.2728	0.7273	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B07:10	HRTEGELQL	0.0095	0.5297	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:02	HRTEGELQLL	0.9998	0.5661	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:04	HRTEGELQLL	0.9998	0.7056	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:05	HRTEGELQLL	0.9998	0.811	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:03	TEGELQLLKY	0.9994	0.9153	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:07	HRTEGELQLL	0.9993	0.6624	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:24	HRTEGELQLL	0.9972	0.6783	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:01	HRTEGELQLL	0.994	0.9238	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A03:12	RTEGELQLLK	0.9905	0.5068	8	18
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:01	LHRTEGELQL	0.9816	0.972	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:24	LHRTEGELQL	0.9811	0.8502	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:02	HRTEGELQLL	0.9796	0.9735	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:01	HRTEGELQLL	0.9739	0.9733	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:01	TEGELQLLKY	0.9729	0.7934	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:10	LHRTEGELQL	0.9214	0.8779	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:01	LHRTEGELQL	0.9156	0.9918	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:02	LHRTEGELQL	0.9102	0.9915	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:37	LHRTEGELQL	0.8757	0.8953	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B07:10	LHRTEGELQL	0.5449	0.6363	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:05	HRTEGELQLLK	0.9996	0.721	7	18
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:01	LHRTEGELQLL	0.9854	0.9901	6	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:07	GLHRTEGELQL	0.9807	0.8736	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:22	GLHRTEGELQL	0.9371	0.5726	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:27	GLHRTEGELQL	0.8228	0.6142	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:60	GLHRTEGELQL	0.6354	0.5468	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:24	GLHRTEGELQL	0.6338	0.5143	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:67	GLHRTEGELQL	0.6338	0.5143	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:30	GLHRTEGELQL	0.6338	0.5143	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:20	GLHRTEGELQL	0.5218	0.5263	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:29	GLHRTEGELQL	0.5083	0.5128	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C05:09	RTEGELQLL	1	0.9779	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C08:15	RTEGELQLL	0.9999	0.9841	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:14	HRTEGELQL	0.9994	0.7923	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:95	HRTEGELQL	0.9985	0.8502	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:09	HRTEGELQL	0.9982	0.8537	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:12	HRTEGELQL	0.9979	0.9285	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:05	HRTEGELQL	0.9978	0.979	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:27	HRTEGELQL	0.9977	0.9738	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:29	HRTEGELQL	0.9957	0.9597	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C15:06	RTEGELQLL	0.9951	0.9705	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:13	HRTEGELQL	0.9936	0.9576	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:03	HRTEGELQL	0.9728	0.8433	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:46	HRTEGELQL	0.9679	0.9537	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:05	HRTEGELQL	0.9581	0.9196	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B73:01	HRTEGELQL	0.9184	0.8667	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:19	HRTEGELQL	0.8715	0.8817	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:10	HRTEGELQL	0.8576	0.9836	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:67	HRTEGELQL	0.8362	0.9711	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:80	HRTEGELQL	0.8362	0.9711	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:08	GELQLLKYL	0.5695	0.7316	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B14:03	HRTEGELQL	0.4729	0.8913	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C12:16	HRTEGELQL	0.058	0.968	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:95	HRTEGELQLL	0.9988	0.8586	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:03	HRTEGELQLL	0.9961	0.8368	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:12	HRTEGELQLL	0.9944	0.9271	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:05	HRTEGELQLL	0.9255	0.9175	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:05	LHRTEGELQL	0.9124	0.9702	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:14	HRTEGELQLLK	0.9992	0.6509	7	18
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:03	HRTEGELQLLK	0.9801	0.7472	7	18
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-A02:01	GLHRTEGELQL	0.6338	0.5143	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:13	GELQLLKY	0.9998	0.8646	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:26	GELQLLKY	0.9998	0.8646	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:07	GELQLLKY	0.9998	0.8646	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B40:04	TEGELQLL	0.9995	0.7539	9	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:08	GELQLLKY	0.9974	0.5974	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:05	GELQLLKY	0.9965	0.6399	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:06	GELQLLKY	0.996	0.6509	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:03	GELQLLKY	0.9949	0.6219	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:11	GELQLLKY	0.991	0.6122	11	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B41:03	TEGELQLL	0.9028	0.6348	9	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C05:01	RTEGELQLL	1	0.9779	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C04:03	RTEGELQLL	1	0.9665	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C08:02	RTEGELQLL	0.9999	0.9841	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:08	HRTEGELQL	0.9994	0.7866	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:10	HRTEGELQL	0.9993	0.8294	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:06	HRTEGELQL	0.9992	0.7354	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:09	HRTEGELQL	0.9991	0.8072	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:01	HRTEGELQL	0.9986	0.8497	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:02	HRTEGELQL	0.9982	0.9714	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:31	HRTEGELQL	0.9972	0.925	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:13	GELQLLKYL	0.9968	0.872	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:26	GELQLLKYL	0.9968	0.872	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:07	GELQLLKYL	0.9968	0.872	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C15:05	RTEGELQLL	0.9942	0.9797	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C15:02	RTEGELQLL	0.9915	0.9725	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:05	HRTEGELQL	0.9902	0.9748	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:17	HRTEGELQL	0.9883	0.9736	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C18:01	HRTEGELQL	0.9684	0.9498	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:08	HRTEGELQL	0.9594	0.9843	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:04	HRTEGELQL	0.8556	0.9574	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:02	HRTEGELQL	0.8362	0.9711	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:06	HRTEGELQL	0.7716	0.9892	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:11	HRTEGELQL	0.7533	0.8055	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:22	HRTEGELQL	0.7459	0.8203	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:09	HRTEGELQL	0.7165	0.912	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C03:67	HRTEGELQL	0.6869	0.9902	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C04:04	HRTEGELQL	0.6567	0.9717	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B41:03	GELQLLKYL	0.5913	0.5064	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B57:02	RTEGELQLL	0.4708	0.9739	8	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:02	GELQLLKYL	0.2091	0.7279	11	20
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:02	HRTEGELQL	0.0774	0.9912	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:17	HRTEGELQL	0.0774	0.9912	7	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:08	HRTEGELQLL	0.9998	0.763	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:09	HRTEGELQLL	0.9996	0.8196	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:06	HRTEGELQLL	0.9995	0.714	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:13	TEGELQLLKY	0.9994	0.9153	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:07	TEGELQLLKY	0.9994	0.9153	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B44:26	TEGELQLLKY	0.9994	0.9153	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:31	HRTEGELQLL	0.9948	0.9235	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:08	HRTEGELQLL	0.9895	0.9812	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C07:22	HRTEGELQLL	0.9891	0.8213	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:05	HRTEGELQLL	0.9739	0.9733	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:05	TEGELQLLKY	0.9729	0.7934	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:09	LHRTEGELQL	0.9645	0.9414	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:05	LHRTEGELQL	0.9156	0.9918	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:17	HRTEGELQLL	0.9024	0.99	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-C06:02	HRTEGELQLL	0.9024	0.99	7	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B18:11	TEGELQLLKY	0.9008	0.7474	9	19
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B39:11	LHRTEGELQL	0.8149	0.909	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B15:09	LHRTEGELQL	0.7473	0.9602	6	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:10	HRTEGELQLLK	0.9995	0.7541	7	18
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:08	GLHRTEGELQL	0.992	0.7412	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:06	GLHRTEGELQL	0.989	0.8408	5	16
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B38:05	LHRTEGELQLL	0.9854	0.9901	6	17
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	380	HLA-B27:09	GLHRTEGELQL	0.9669	0.8224	5	16

Top

Potential FusionNeoAntigen Information of SLC25A25-TCEB3 in HLA II

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene

Hchr

Hbp

Tgene

Tchr

Tbp

HLA II

FusionNeoAntigen peptide

Neoantigen start (at BP 13)

Neoantigen end (at BP 13)

Top

Fusion breakpoint peptide structures of SLC25A25-TCEB3

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
5071	LHRTEGELQLLKYL	SLC25A25	TCEB3	chr9	130830859	chr1	24075511	380

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SLC25A25-TCEB3

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	5071	LHRTEGELQLLKYL	-7.9962	-8.1096
HLA-B14:02	3BVN	5071	LHRTEGELQLLKYL	-5.70842	-6.74372
HLA-B52:01	3W39	5071	LHRTEGELQLLKYL	-6.83737	-6.95077
HLA-B52:01	3W39	5071	LHRTEGELQLLKYL	-4.4836	-5.5189
HLA-A11:01	4UQ2	5071	LHRTEGELQLLKYL	-10.0067	-10.1201
HLA-A11:01	4UQ2	5071	LHRTEGELQLLKYL	-9.03915	-10.0745
HLA-A24:02	5HGA	5071	LHRTEGELQLLKYL	-6.56204	-6.67544
HLA-A24:02	5HGA	5071	LHRTEGELQLLKYL	-5.42271	-6.45801
HLA-B44:05	3DX8	5071	LHRTEGELQLLKYL	-7.85648	-8.89178
HLA-B44:05	3DX8	5071	LHRTEGELQLLKYL	-5.3978	-5.5112
HLA-A02:01	6TDR	5071	LHRTEGELQLLKYL	-3.37154	-4.40684

Top

Vaccine Design for the FusionNeoAntigens of SLC25A25-TCEB3

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	11	19	GELQLLKY	CTCCAGCTATTGAAATATTTGAAG
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	11	20	GELQLLKYL	CTCCAGCTATTGAAATATTTGAAGAAA
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	5	16	GLHRTEGELQL	CACCGCACCGAGGGCGAGCTCCAGCTATTGAAA
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	6	16	LHRTEGELQL	CGCACCGAGGGCGAGCTCCAGCTATTGAAA
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	6	17	LHRTEGELQLL	CGCACCGAGGGCGAGCTCCAGCTATTGAAATAT
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	7	16	HRTEGELQL	ACCGAGGGCGAGCTCCAGCTATTGAAA
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	7	17	HRTEGELQLL	ACCGAGGGCGAGCTCCAGCTATTGAAATAT
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	7	18	HRTEGELQLLK	ACCGAGGGCGAGCTCCAGCTATTGAAATATTTG
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	8	17	RTEGELQLL	GAGGGCGAGCTCCAGCTATTGAAATAT
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	8	18	RTEGELQLLK	GAGGGCGAGCTCCAGCTATTGAAATATTTG
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	9	17	TEGELQLL	GGCGAGCTCCAGCTATTGAAATAT
SLC25A25-TCEB3	chr9	130830859	chr1	24075511	9	19	TEGELQLLKY	GGCGAGCTCCAGCTATTGAAATATTTGAAG

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene

Hchr

Hbp

Tchr

Tbp

Start in +/-13AA

End in +/-13AA

FusionNeoAntigen peptide

FusionNEoAntigen RNA sequence

Top

Information of the samples that have these potential fusion neoantigens of SLC25A25-TCEB3

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
UCEC	SLC25A25-TCEB3	chr9	130830859	ENST00000373068	chr1	24075511	ENST00000418390	TCGA-PG-A7D5-01A

Top

Potential target of CAR-T therapy development for SLC25A25-TCEB3

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to SLC25A25-TCEB3

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to SLC25A25-TCEB3

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)