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Fusion Protein:SLC26A6-CYP2C8 |
Fusion Gene and Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: SLC26A6-CYP2C8 | FusionPDB ID: 82761 | FusionGDB2.0 ID: 82761 | Hgene | Tgene | Gene symbol | SLC26A6 | CYP2C8 | Gene ID | 65010 | 1558 |
| Gene name | solute carrier family 26 member 6 | cytochrome P450 family 2 subfamily C member 8 | |
| Synonyms | - | CPC8|CYP2C8DM|CYPIIC8|MP-12/MP-20 | |
| Cytomap | 3p21.31 | 10q23.33 | |
| Type of gene | protein-coding | protein-coding | |
| Description | solute carrier family 26 member 6anion exchange transporteranion transporter 1pendrin L1solute carrier family 26 (anion exchanger), member 6sulfate anion transporter | cytochrome P450 2C8P450 form 1cytochrome P450 IIC2cytochrome P450 MP-12cytochrome P450 MP-20cytochrome P450 form 1cytochrome P450, family 2, subfamily C, polypeptide 8cytochrome P450, subfamily IIC (mephenytoin 4-hydroxylase), polypeptide 8flavopr | |
| Modification date | 20200329 | 20200320 | |
| UniProtAcc | Q9BXS9 Main function of 5'-partner protein: FUNCTION: Apical membrane anion-exchanger with wide epithelial distribution that plays a role as a component of the pH buffering system for maintaining acid-base homeostasis. Acts as a versatile DIDS-sensitive inorganic and organic anion transporter that mediates the uptake of monovalent anions like chloride, bicarbonate, formate and hydroxyl ion and divalent anions like sulfate and oxalate. Functions in multiple exchange modes involving pairs of these anions, which include chloride-bicarbonate, chloride-oxalate, oxalate-formate, oxalate-sulfate and chloride-formate exchange. Apical membrane chloride-bicarbonate exchanger that mediates luminal chloride absorption and bicarbonate secretion by the small intestinal brush border membrane and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption, possibly by providing a bicarbonate import pathway. Mediates also intestinal chloride absorption and oxalate secretion, thereby preventing hyperoxaluria and calcium oxalate urolithiasis. Transepithelial oxalate secretion, chloride-formate, chloride-oxalate and chloride-bicarbonate transport activities in the duodenum are inhibited by PKC activation in a calcium-independent manner. The apical membrane chloride-bicarbonate exchanger provides also a major route for fluid and bicarbonate secretion into the proximal tubules of the kidney as well as into the proximal part of the interlobular pancreatic ductal tree, where it mediates electrogenic chloride-bicarbonate exchange with a chloride-bicarbonate stoichiometry of 1:2, and hence will dilute and alkalinize protein-rich acinar secretion. Mediates also the transcellular sulfate absorption and oxalate secretion across the apical membrane in the duodenum and the formate ion efflux at the apical brush border of cells in the proximal tubules of kidney. Plays a role in sperm capacitation by increasing intracellular pH.; FUNCTION: [Isoform 4]: Apical membrane chloride-bicarbonate exchanger. Its association with carbonic anhydrase CA2 forms a bicarbonate transport metabolon; hence maximizes the local concentration of bicarbonate at the transporter site. | P10632 Main function of 5'-partner protein: FUNCTION: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:7574697, PubMed:11093772, PubMed:14559847, PubMed:15766564, PubMed:19965576). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:7574697, PubMed:11093772, PubMed:14559847, PubMed:15766564, PubMed:19965576). Primarily catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) with a preference for the last double bond (PubMed:7574697, PubMed:15766564, PubMed:19965576). Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes all trans-retinoic acid toward its 4-hydroxylated form (PubMed:11093772). Displays 16-alpha hydroxylase activity toward estrogen steroid hormones, 17beta-estradiol (E2) and estrone (E1) (PubMed:14559847). Plays a role in the oxidative metabolism of xenobiotics. It is the principal enzyme responsible for the metabolism of the anti-cancer drug paclitaxel (taxol) (PubMed:26427316). {ECO:0000269|PubMed:11093772, ECO:0000269|PubMed:14559847, ECO:0000269|PubMed:15766564, ECO:0000269|PubMed:19965576, ECO:0000269|PubMed:26427316, ECO:0000269|PubMed:7574697}. | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000337000, ENST00000383733, ENST00000395550, ENST00000420764, ENST00000455886, ENST00000358747, ENST00000482282, | ENST00000539050, ENST00000371270, ENST00000535898, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 2 X 2 X 2=8 | 6 X 2 X 5=60 |
| # samples | 2 | 6 | |
| ** MAII score | log2(2/8*10)=1.32192809488736 | log2(6/60*10)=0 | |
| Fusion gene context | PubMed: SLC26A6 [Title/Abstract] AND CYP2C8 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Fusion neoantigen context | PubMed: SLC26A6 [Title/Abstract] AND CYP2C8 [Title/Abstract] AND neoantigen [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | SLC26A6(48672804)-CYP2C8(96798795), # samples:3 | ||
| Anticipated loss of major functional domain due to fusion event. | SLC26A6-CYP2C8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SLC26A6-CYP2C8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | SLC26A6 | GO:0071346 | cellular response to interferon-gamma | 18655181 |
| Tgene | CYP2C8 | GO:0002933 | lipid hydroxylation | 14559847 |
| Tgene | CYP2C8 | GO:0006082 | organic acid metabolic process | 19651758 |
| Tgene | CYP2C8 | GO:0008202 | steroid metabolic process | 14559847 |
| Tgene | CYP2C8 | GO:0008210 | estrogen metabolic process | 12865317|14559847 |
| Tgene | CYP2C8 | GO:0017144 | drug metabolic process | 19651758 |
| Tgene | CYP2C8 | GO:0019373 | epoxygenase P450 pathway | 7574697 |
| Tgene | CYP2C8 | GO:0042573 | retinoic acid metabolic process | 11093772 |
| Tgene | CYP2C8 | GO:0042738 | exogenous drug catabolic process | 18619574 |
| Tgene | CYP2C8 | GO:0046456 | icosanoid biosynthetic process | 15766564 |
| Tgene | CYP2C8 | GO:0055114 | oxidation-reduction process | 19651758 |
| Tgene | CYP2C8 | GO:0070989 | oxidative demethylation | 18619574 |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr3:48672804/chr10:96798795) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here. |
| Fusion gene breakpoints across SLC26A6 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across CYP2C8 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000420764 | SLC26A6 | chr3 | 48672804 | - | ENST00000371270 | CYP2C8 | chr10 | 96798795 | - | 792 | 113 | 570 | 232 | 112 |
| ENST00000420764 | SLC26A6 | chr3 | 48672804 | - | ENST00000535898 | CYP2C8 | chr10 | 96798795 | - | 581 | 113 | 570 | 232 | 112 |
| ENST00000395550 | SLC26A6 | chr3 | 48672804 | - | ENST00000371270 | CYP2C8 | chr10 | 96798795 | - | 750 | 71 | 528 | 190 | 112 |
| ENST00000395550 | SLC26A6 | chr3 | 48672804 | - | ENST00000535898 | CYP2C8 | chr10 | 96798795 | - | 539 | 71 | 528 | 190 | 112 |
| ENST00000383733 | SLC26A6 | chr3 | 48672804 | - | ENST00000371270 | CYP2C8 | chr10 | 96798795 | - | 763 | 84 | 541 | 203 | 112 |
| ENST00000383733 | SLC26A6 | chr3 | 48672804 | - | ENST00000535898 | CYP2C8 | chr10 | 96798795 | - | 552 | 84 | 541 | 203 | 112 |
| ENST00000337000 | SLC26A6 | chr3 | 48672804 | - | ENST00000371270 | CYP2C8 | chr10 | 96798795 | - | 802 | 123 | 580 | 242 | 112 |
| ENST00000337000 | SLC26A6 | chr3 | 48672804 | - | ENST00000535898 | CYP2C8 | chr10 | 96798795 | - | 591 | 123 | 580 | 242 | 112 |
| ENST00000455886 | SLC26A6 | chr3 | 48672804 | - | ENST00000371270 | CYP2C8 | chr10 | 96798795 | - | 750 | 71 | 528 | 190 | 112 |
| ENST00000455886 | SLC26A6 | chr3 | 48672804 | - | ENST00000535898 | CYP2C8 | chr10 | 96798795 | - | 539 | 71 | 528 | 190 | 112 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000420764 | ENST00000371270 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.015869226 | 0.9841308 |
| ENST00000420764 | ENST00000535898 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.006097825 | 0.9939022 |
| ENST00000395550 | ENST00000371270 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.021064755 | 0.9789353 |
| ENST00000395550 | ENST00000535898 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.010074382 | 0.9899256 |
| ENST00000383733 | ENST00000371270 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.021136662 | 0.9788633 |
| ENST00000383733 | ENST00000535898 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.008571793 | 0.9914282 |
| ENST00000337000 | ENST00000371270 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.019619552 | 0.9803805 |
| ENST00000337000 | ENST00000535898 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.010209348 | 0.9897906 |
| ENST00000455886 | ENST00000371270 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.021064755 | 0.9789353 |
| ENST00000455886 | ENST00000535898 | SLC26A6 | chr3 | 48672804 | - | CYP2C8 | chr10 | 96798795 | - | 0.010074382 | 0.9899256 |
| Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones. |
Get the fusion protein sequences from here. |
| Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for SLC26A6-CYP2C8 |
| +/-13 AA sequence from the breakpoints of the fusion protein sequences. |
| Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
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Potential FusionNeoAntigen Information of SLC26A6-CYP2C8 in HLA I |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Potential FusionNeoAntigen Information of SLC26A6-CYP2C8 in HLA II |
| Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific. |
| Potential FusionNeoAntigen Information * We used NetMHCIIpan v4.1 (%rank<0.5). |
| Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
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Fusion breakpoint peptide structures of SLC26A6-CYP2C8 |
| 3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SLC26A6-CYP2C8 |
| Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens * We used Glide to predict the interaction between HLAs and neoantigens. |
| HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
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Vaccine Design for the FusionNeoAntigens of SLC26A6-CYP2C8 |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
| mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs. |
| Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
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Information of the samples that have these potential fusion neoantigens of SLC26A6-CYP2C8 |
| These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens. |
| Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
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Potential target of CAR-T therapy development for SLC26A6-CYP2C8 |
| Predicted 3D structure. We used RoseTTAFold. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features * Minus value of BPloci means that the break point is located before the CDS. |
| - In-frame and retained 'Transmembrane'. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Subcellular localization prediction of the transmembrane domain retained fusion proteins * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
| Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to SLC26A6-CYP2C8 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to SLC26A6-CYP2C8 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | SLC26A6 | C2239176 | Liver carcinoma | 1 | CTD_human |
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