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Fusion Protein:SPEN-CERS4

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SPEN-CERS4
	FusionPDB ID: 85713
	FusionGDB2.0 ID: 85713
		Hgene	Tgene
	Gene symbol	SPEN	CERS4
	Gene ID	64783	79603
	Gene name	RNA binding motif protein 15	ceramide synthase 4
	Synonyms	OTT\|OTT1\|SPEN	LASS4\|Trh1
	Cytomap	1p13.3	19p13.2
	Type of gene	protein-coding	protein-coding
	Description	RNA-binding protein 15one twenty two proteinone twenty-twoone-twenty two protein 1putative RNA-binding protein 15	ceramide synthase 4LAG1 homolog, ceramide synthase 4LAG1 longevity assurance homolog 4sphingosine N-acyltransferase CERS4
	Modification date	20200313	20200320
	UniProtAcc	.	Q9HA82 Main function of 5'-partner protein: FUNCTION: Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward long and very-long chains (C18:0-C22:0) as acyl donor. {ECO:0000269\|PubMed:17977534, ECO:0000269\|PubMed:23530041, ECO:0000269\|PubMed:26887952, ECO:0000269\|PubMed:29632068, ECO:0000269\|PubMed:31916624}.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000375759, ENST00000471538,	ENST00000595722, ENST00000251363, ENST00000559336, ENST00000559450, ENST00000558331,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	20 X 12 X 15=3600	11 X 14 X 5=770
	# samples	31	15
	** MAII score	log2(31/3600*10)=-3.5376567859428 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(15/770*10)=-2.35989594508638 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: SPEN [Title/Abstract] AND CERS4 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: SPEN [Title/Abstract] AND CERS4 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SPEN(16174645)-CERS4(8315960), # samples:3
Anticipated loss of major functional domain due to fusion event.	SPEN-CERS4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SPEN-CERS4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SPEN-CERS4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SPEN-CERS4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	SPEN	GO:0000381	regulation of alternative mRNA splicing, via spliceosome	26575292
Hgene	SPEN	GO:0001510	RNA methylation	27602518
Hgene	SPEN	GO:0009048	dosage compensation by inactivation of X chromosome	27602518
Hgene	SPEN	GO:0045652	regulation of megakaryocyte differentiation	26575292
Hgene	SPEN	GO:0045892	negative regulation of transcription, DNA-templated	16129689
Tgene	CERS4	GO:0046513	ceramide biosynthetic process	17977534\|29632068

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:16174645/chr19:8315960)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across SPEN (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CERS4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000375759	SPEN	chr1	16174645	+	ENST00000558331	CERS4	chr19	8319382	+	1600	287	27	1298	423
ENST00000375759	SPEN	chr1	16174645	+	ENST00000251363	CERS4	chr19	8319382	+	1583	287	27	1298	423
ENST00000375759	SPEN	chr1	16174645	+	ENST00000559450	CERS4	chr19	8319382	+	1331	287	27	1298	423
ENST00000375759	SPEN	chr1	16174645	+	ENST00000559336	CERS4	chr19	8319382	+	1347	287	27	1034	335
ENST00000375759	SPEN	chr1	16174645	+	ENST00000558331	CERS4	chr19	8319383	+	1600	287	27	1298	423
ENST00000375759	SPEN	chr1	16174645	+	ENST00000251363	CERS4	chr19	8319383	+	1583	287	27	1298	423
ENST00000375759	SPEN	chr1	16174645	+	ENST00000559450	CERS4	chr19	8319383	+	1331	287	27	1298	423
ENST00000375759	SPEN	chr1	16174645	+	ENST00000559336	CERS4	chr19	8319383	+	1347	287	27	1034	335
ENST00000375759	SPEN	chr1	16174644	+	ENST00000558331	CERS4	chr19	8319382	+	1600	287	27	1298	423
ENST00000375759	SPEN	chr1	16174644	+	ENST00000251363	CERS4	chr19	8319382	+	1583	287	27	1298	423
ENST00000375759	SPEN	chr1	16174644	+	ENST00000559450	CERS4	chr19	8319382	+	1331	287	27	1298	423
ENST00000375759	SPEN	chr1	16174644	+	ENST00000559336	CERS4	chr19	8319382	+	1347	287	27	1034	335

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000375759	ENST00000558331	SPEN	chr1	16174645	+	CERS4	chr19	8319382	+	0.033923678	0.9660763
ENST00000375759	ENST00000251363	SPEN	chr1	16174645	+	CERS4	chr19	8319382	+	0.035673495	0.9643265
ENST00000375759	ENST00000559450	SPEN	chr1	16174645	+	CERS4	chr19	8319382	+	0.029621514	0.9703785
ENST00000375759	ENST00000559336	SPEN	chr1	16174645	+	CERS4	chr19	8319382	+	0.068058744	0.9319412
ENST00000375759	ENST00000558331	SPEN	chr1	16174645	+	CERS4	chr19	8319383	+	0.033923678	0.9660763
ENST00000375759	ENST00000251363	SPEN	chr1	16174645	+	CERS4	chr19	8319383	+	0.035673495	0.9643265
ENST00000375759	ENST00000559450	SPEN	chr1	16174645	+	CERS4	chr19	8319383	+	0.029621514	0.9703785
ENST00000375759	ENST00000559336	SPEN	chr1	16174645	+	CERS4	chr19	8319383	+	0.068058744	0.9319412
ENST00000375759	ENST00000558331	SPEN	chr1	16174644	+	CERS4	chr19	8319382	+	0.033923678	0.9660763
ENST00000375759	ENST00000251363	SPEN	chr1	16174644	+	CERS4	chr19	8319382	+	0.035673495	0.9643265
ENST00000375759	ENST00000559450	SPEN	chr1	16174644	+	CERS4	chr19	8319382	+	0.029621514	0.9703785
ENST00000375759	ENST00000559336	SPEN	chr1	16174644	+	CERS4	chr19	8319382	+	0.068058744	0.9319412

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for SPEN-CERS4

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
SPEN	chr1	16174644	CERS4	chr19	8319382	287	86	ENVREEKIIEHFKRFIGLPLSRWLGV
SPEN	chr1	16174645	CERS4	chr19	8319382	287	86	ENVREEKIIEHFKRFIGLPLSRWLGV
SPEN	chr1	16174645	CERS4	chr19	8319383	287	86	ENVREEKIIEHFKRFIGLPLSRWLGV

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Potential FusionNeoAntigen Information of SPEN-CERS4 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

SPEN-CERS4_16174644_8319382.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:04	KRFIGLPL	1	0.7709	12	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:01	EHFKRFIGL	0.999	0.685	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:06	EHFKRFIGL	0.9988	0.5101	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B38:02	EHFKRFIGL	0.9968	0.8077	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B38:01	EHFKRFIGL	0.9962	0.8255	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B58:02	KIIEHFKRF	0.9961	0.7587	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:17	KIIEHFKRF	0.9957	0.7381	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:16	KIIEHFKRF	0.9923	0.5873	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:25	KIIEHFKRF	0.9905	0.8124	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:01	KIIEHFKRF	0.9859	0.8276	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A32:13	KIIEHFKRF	0.9777	0.9484	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:03	KIIEHFKRF	0.9318	0.6063	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A30:08	KIIEHFKRF	0.7415	0.7542	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B13:01	KIIEHFKRF	0.0224	0.7679	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:05	KRFIGLPLSR	1	0.9239	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:02	KRFIGLPLSR	1	0.7689	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:04	KRFIGLPLSR	0.9998	0.8472	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A31:02	KRFIGLPLSR	0.9033	0.7373	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A31:06	KRFIGLPLSR	0.6422	0.5319	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:02	KRFIGLPLSRW	1	0.8895	12	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:05	KRFIGLPLSRW	1	0.9647	12	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:01	EHFKRFIGLPL	0.9986	0.6733	9	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B14:01	EHFKRFIGLPL	0.9984	0.6178	9	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B14:02	EHFKRFIGLPL	0.9984	0.6178	9	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B44:03	EEKIIEHFKRF	0.9982	0.7757	4	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A31:08	KRFIGLPLSRW	0.9909	0.8542	12	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:14	KRFIGLPL	1	0.7844	12	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:14	KRFIGLPLS	0.9993	0.8616	12	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:12	EHFKRFIGL	0.9989	0.6971	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:05	EHFKRFIGL	0.9963	0.6443	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:07	KIIEHFKRF	0.9901	0.5649	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C15:04	KIIEHFKRF	0.96	0.8322	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:04	KIIEHFKRF	0.9343	0.8383	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:05	KIIEHFKRF	0.8462	0.7693	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C12:12	KIIEHFKRF	0.8188	0.9454	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C07:13	EHFKRFIGL	0.8143	0.7986	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C12:04	KIIEHFKRF	0.7159	0.996	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C06:03	KIIEHFKRF	0.7157	0.9953	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C03:14	KIIEHFKRF	0.5594	0.9835	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B14:03	EHFKRFIGL	0.522	0.5531	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:14	KRFIGLPLSR	1	0.8798	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:03	KRFIGLPLSR	0.999	0.9393	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A31:01	KRFIGLPLSR	0.895	0.7277	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:14	KRFIGLPLSRW	1	0.9493	12	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:03	KRFIGLPLSRW	0.9996	0.9668	12	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:06	KRFIGLPL	1	0.7849	12	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:08	KRFIGLPL	1	0.7855	12	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:10	KRFIGLPL	1	0.9113	12	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:09	KRFIGLPL	0.9999	0.894	12	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:31	EHFKRFIGL	0.999	0.6856	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:02	EHFKRFIGL	0.9989	0.7295	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B58:06	KIIEHFKRF	0.9968	0.5516	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B38:05	EHFKRFIGL	0.9962	0.8255	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A32:01	KIIEHFKRF	0.9958	0.9645	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:39	KIIEHFKRF	0.9903	0.6785	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:24	KIIEHFKRF	0.9898	0.8203	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:35	KIIEHFKRF	0.9895	0.7988	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:27	KIIEHFKRF	0.9873	0.839	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:33	KIIEHFKRF	0.9859	0.8276	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:125	KIIEHFKRF	0.9859	0.8276	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:34	KIIEHFKRF	0.9859	0.8276	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:135	KIIEHFKRF	0.9845	0.815	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:50	KIIEHFKRF	0.9794	0.9174	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C15:09	KIIEHFKRF	0.96	0.8322	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:12	KIIEHFKRF	0.9318	0.8256	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C03:02	KIIEHFKRF	0.874	0.9345	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:20	KIIEHFKRF	0.8517	0.8064	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C16:02	KIIEHFKRF	0.837	0.9918	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B39:11	EHFKRFIGL	0.7854	0.6343	9	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A25:01	KIIEHFKRF	0.7101	0.8049	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C12:02	KIIEHFKRF	0.6838	0.9754	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C02:02	KIIEHFKRF	0.1361	0.93	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-C02:10	KIIEHFKRF	0.1361	0.93	6	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:10	KRFIGLPLSR	0.9999	0.9121	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:08	KRFIGLPLSR	0.9999	0.8732	12	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-A25:01	EKIIEHFKRF	0.4383	0.8047	5	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B27:10	KRFIGLPLSRW	0.9999	0.9692	12	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B44:26	EEKIIEHFKRF	0.9982	0.7757	4	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B44:13	EEKIIEHFKRF	0.9982	0.7757	4	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B44:07	EEKIIEHFKRF	0.9982	0.7757	4	15
SPEN-CERS4	chr1	16174644	chr19	8319382	287	HLA-B15:09	EHFKRFIGLPL	0.9874	0.55	9	20

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Potential FusionNeoAntigen Information of SPEN-CERS4 in HLA II

SPEN-CERS4_16174644_8319382.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0103	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0103	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0109	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0109	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0115	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0115	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0810	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0812	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0828	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-0828	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1102	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1102	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1102	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1103	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1103	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1103	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1104	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1106	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1111	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1113	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1114	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1116	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1116	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1116	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1118	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1118	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1120	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1121	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1121	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1121	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1125	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1125	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1135	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1136	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1136	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1136	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1138	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1141	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1141	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1142	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1142	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1143	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1144	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1146	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1147	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1148	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1148	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1148	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1155	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1155	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1155	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1157	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1158	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1159	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1159	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1160	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1163	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1163	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1163	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1165	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1165	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1165	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1167	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1167	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1168	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1169	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1170	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1170	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1170	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1176	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1176	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1176	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1177	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1178	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1182	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1185	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1185	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1185	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1189	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1192	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1192	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1301	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1301	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1301	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1302	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1304	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1304	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1304	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1306	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1306	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1308	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1308	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1308	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1309	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1309	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1309	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1311	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1315	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1315	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1315	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1317	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1317	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1317	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1318	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1318	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1319	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1319	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1319	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1320	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1320	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1320	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1322	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1322	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1322	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1323	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1324	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1324	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1324	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1327	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1327	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1329	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1331	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1332	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1332	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1334	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1335	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1335	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1335	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1336	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1339	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1342	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1343	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1343	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1343	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1348	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1348	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1351	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1351	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1351	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1352	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1352	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1352	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1353	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1353	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1353	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1354	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1354	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1354	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1354	VREEKIIEHFKRFIG	2	17
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1357	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1357	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1357	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1359	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1359	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1359	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1361	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1361	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1361	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1363	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1364	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1364	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1364	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1368	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1368	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1368	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1369	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1369	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1369	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1370	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1370	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1370	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1371	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1371	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1372	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1372	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1372	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1373	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1374	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1375	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1375	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1376	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1376	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1376	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1377	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1378	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1378	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1378	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1379	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1379	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1379	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1380	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1380	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1380	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1383	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1383	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1383	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1384	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1384	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1384	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1387	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1387	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1387	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1391	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1391	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1391	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1392	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1392	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1392	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1393	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1393	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1393	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1397	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1398	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1398	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1398	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1399	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1412	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1412	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1416	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1416	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1416	REEKIIEHFKRFIGL	3	18
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1437	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1437	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1465	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1478	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1478	EKIIEHFKRFIGLPL	5	20
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1481	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1484	EEKIIEHFKRFIGLP	4	19
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1502	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1502	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1502	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1502	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1508	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1508	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1508	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1508	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1511	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1511	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1511	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1511	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1514	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1514	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1514	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1514	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1515	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1515	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1515	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1515	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1519	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1519	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1519	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1519	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1526	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1526	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1526	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1526	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1527	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1527	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1527	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1527	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1529	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1529	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1529	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1529	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1530	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1530	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1530	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1530	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1531	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1531	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1531	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1531	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1534	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1534	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1534	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1538	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1538	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1538	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1538	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1539	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1539	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1539	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1539	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1544	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1544	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1544	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1544	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1547	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1547	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1547	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1547	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1601	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1601	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1602	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1602	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1603	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1603	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1604	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1604	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1604	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1605	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1605	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1605	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1607	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1607	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1607	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1608	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1608	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1609	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1609	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1609	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1609	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1610	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1610	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1610	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1610	EHFKRFIGLPLSRWL	9	24
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1611	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1611	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1612	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1612	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1612	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1614	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1614	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1614	KIIEHFKRFIGLPLS	6	21
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1616	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB1-1616	IEHFKRFIGLPLSRW	8	23
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB5-0103	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB5-0203	IIEHFKRFIGLPLSR	7	22
SPEN-CERS4	chr1	16174644	chr19	8319382	287	DRB5-0203	IEHFKRFIGLPLSRW	8	23

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Fusion breakpoint peptide structures of SPEN-CERS4

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
4301	KIIEHFKRFIGLPL	SPEN	CERS4	chr1	16174644	chr19	8319382	287

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SPEN-CERS4

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	4301	KIIEHFKRFIGLPL	-7.9962	-8.1096
HLA-B14:02	3BVN	4301	KIIEHFKRFIGLPL	-5.70842	-6.74372
HLA-B52:01	3W39	4301	KIIEHFKRFIGLPL	-6.83737	-6.95077
HLA-B52:01	3W39	4301	KIIEHFKRFIGLPL	-4.4836	-5.5189
HLA-A11:01	4UQ2	4301	KIIEHFKRFIGLPL	-10.0067	-10.1201
HLA-A11:01	4UQ2	4301	KIIEHFKRFIGLPL	-9.03915	-10.0745
HLA-A24:02	5HGA	4301	KIIEHFKRFIGLPL	-6.56204	-6.67544
HLA-A24:02	5HGA	4301	KIIEHFKRFIGLPL	-5.42271	-6.45801
HLA-B44:05	3DX8	4301	KIIEHFKRFIGLPL	-7.85648	-8.89178
HLA-B44:05	3DX8	4301	KIIEHFKRFIGLPL	-5.3978	-5.5112
HLA-A02:01	6TDR	4301	KIIEHFKRFIGLPL	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of SPEN-CERS4

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
SPEN-CERS4	chr1	16174644	chr19	8319382	12	20	KRFIGLPL	ACGATTCATTGGCCTGCCCCTGAG
SPEN-CERS4	chr1	16174644	chr19	8319382	12	21	KRFIGLPLS	ACGATTCATTGGCCTGCCCCTGAGCCG
SPEN-CERS4	chr1	16174644	chr19	8319382	12	22	KRFIGLPLSR	ACGATTCATTGGCCTGCCCCTGAGCCGGTG
SPEN-CERS4	chr1	16174644	chr19	8319382	12	23	KRFIGLPLSRW	ACGATTCATTGGCCTGCCCCTGAGCCGGTGGCT
SPEN-CERS4	chr1	16174644	chr19	8319382	4	15	EEKIIEHFKRF	AGAGAAGATCATCGAGCATTTCAAACGATTCAT
SPEN-CERS4	chr1	16174644	chr19	8319382	5	15	EKIIEHFKRF	GAAGATCATCGAGCATTTCAAACGATTCAT
SPEN-CERS4	chr1	16174644	chr19	8319382	6	15	KIIEHFKRF	GATCATCGAGCATTTCAAACGATTCAT
SPEN-CERS4	chr1	16174644	chr19	8319382	9	18	EHFKRFIGL	GCATTTCAAACGATTCATTGGCCTGCC
SPEN-CERS4	chr1	16174644	chr19	8319382	9	20	EHFKRFIGLPL	GCATTTCAAACGATTCATTGGCCTGCCCCTGAG

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
SPEN-CERS4	chr1	16174644	chr19	8319382	2	17	VREEKIIEHFKRFIG	GCGGGAAGAGAAGATCATCGAGCATTTCAAACGATTCATTGGCCT
SPEN-CERS4	chr1	16174644	chr19	8319382	3	18	REEKIIEHFKRFIGL	GGAAGAGAAGATCATCGAGCATTTCAAACGATTCATTGGCCTGCC
SPEN-CERS4	chr1	16174644	chr19	8319382	4	19	EEKIIEHFKRFIGLP	AGAGAAGATCATCGAGCATTTCAAACGATTCATTGGCCTGCCCCT
SPEN-CERS4	chr1	16174644	chr19	8319382	5	20	EKIIEHFKRFIGLPL	GAAGATCATCGAGCATTTCAAACGATTCATTGGCCTGCCCCTGAG
SPEN-CERS4	chr1	16174644	chr19	8319382	6	21	KIIEHFKRFIGLPLS	GATCATCGAGCATTTCAAACGATTCATTGGCCTGCCCCTGAGCCG
SPEN-CERS4	chr1	16174644	chr19	8319382	7	22	IIEHFKRFIGLPLSR	CATCGAGCATTTCAAACGATTCATTGGCCTGCCCCTGAGCCGGTG
SPEN-CERS4	chr1	16174644	chr19	8319382	8	23	IEHFKRFIGLPLSRW	CGAGCATTTCAAACGATTCATTGGCCTGCCCCTGAGCCGGTGGCT
SPEN-CERS4	chr1	16174644	chr19	8319382	9	24	EHFKRFIGLPLSRWL	GCATTTCAAACGATTCATTGGCCTGCCCCTGAGCCGGTGGCTGGG

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Information of the samples that have these potential fusion neoantigens of SPEN-CERS4

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
LUAD	SPEN-CERS4	chr1	16174644	ENST00000375759	chr19	8319382	ENST00000251363	TCGA-38-4630-01A

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Potential target of CAR-T therapy development for SPEN-CERS4

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000251363

140_160

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000251363

179_199

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000251363

209_229

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000251363

260_280

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000251363

304_324

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000559450

140_160

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000559450

179_199

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000559450

209_229

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000559450

260_280

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174644

chr19:8319382

ENST00000559450

304_324

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000251363

140_160

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000251363

179_199

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000251363

209_229

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000251363

260_280

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000251363

304_324

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000559450

140_160

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000559450

179_199

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000559450

209_229

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000559450

260_280

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319382

ENST00000559450

304_324

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000251363

140_160

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000251363

179_199

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000251363

209_229

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000251363

260_280

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000251363

304_324

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000559450

140_160

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000559450

179_199

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000559450

209_229

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000559450

260_280

395.0

Transmembrane

Helical

Tgene

CERS4

chr1:16174645

chr19:8319383

ENST00000559450

304_324

395.0

Transmembrane

Helical

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to SPEN-CERS4

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to SPEN-CERS4

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)