FusionNeoAntigen Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Fusion Gene and Fusion Protein Summary

leaf

Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

leaf

Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

leaf

Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

leaf

Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

leaf

Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

leaf

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

leaf

Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

leaf

Potential target of CAR-T therapy development

leaf

Information on the samples that have these potential fusion neoantigens

leaf

Fusion Protein Targeting Drugs - (Manual Curation)

leaf

Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:TBC1D14-HTRA3

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: TBC1D14-HTRA3
FusionPDB ID: 89209
FusionGDB2.0 ID: 89209
HgeneTgene
Gene symbol

TBC1D14

HTRA3

Gene ID

57533

94031

Gene nameTBC1 domain family member 14HtrA serine peptidase 3
Synonyms-Prsp|Tasp
Cytomap

4p16.1

4p16.1

Type of geneprotein-codingprotein-coding
DescriptionTBC1 domain family member 14serine protease HTRA3high-temperature requirement factor A3pregnancy-related serine proteaseprobable serine protease HTRA3
Modification date2020031320200313
UniProtAcc.

P83110

Main function of 5'-partner protein: FUNCTION: Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724). {ECO:0000250|UniProtKB:Q9D236, ECO:0000269|PubMed:21321049, ECO:0000269|PubMed:22229724}.
Ensembl transtripts involved in fusion geneENST idsENST00000409757, ENST00000448507, 
ENST00000410031, ENST00000451522, 
ENST00000446947, 		ENST00000307358, 
ENST00000382512, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score8 X 5 X 9=3602 X 3 X 3=18
# samples 134
** MAII scorelog2(13/360*10)=-1.46948528330122
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(4/18*10)=1.15200309344505
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Fusion gene context

PubMed: TBC1D14 [Title/Abstract] AND HTRA3 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: TBC1D14 [Title/Abstract] AND HTRA3 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)TBC1D14(6996029)-HTRA3(8288288), # samples:3
Anticipated loss of major functional domain due to fusion event.TBC1D14-HTRA3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
TBC1D14-HTRA3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
TBC1D14-HTRA3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
TBC1D14-HTRA3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
TBC1D14-HTRA3 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
TBC1D14-HTRA3 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneHTRA3

GO:0006508

proteolysis

18387192|22229724



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr4:6996029/chr4:8288288)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across TBC1D14 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across HTRA3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000448507TBC1D14chr46925838+ENST00000307358HTRA3chr48288288+2653801791677532
ENST00000448507TBC1D14chr46925838+ENST00000382512HTRA3chr48288288+2147801791389436
ENST00000409757TBC1D14chr46925838+ENST00000307358HTRA3chr48288288+2698846581722554
ENST00000409757TBC1D14chr46925838+ENST00000382512HTRA3chr48288288+2192846581434458
ENST00000448507TBC1D14chr46996029+ENST00000307358HTRA3chr48288288+28931041791917612
ENST00000448507TBC1D14chr46996029+ENST00000382512HTRA3chr48288288+23871041791629516
ENST00000409757TBC1D14chr46996029+ENST00000307358HTRA3chr48288288+29381086581962634
ENST00000409757TBC1D14chr46996029+ENST00000382512HTRA3chr48288288+24321086581674538
ENST00000410031TBC1D14chr46996029+ENST00000307358HTRA3chr48288288+2211359421235397
ENST00000410031TBC1D14chr46996029+ENST00000382512HTRA3chr48288288+170535942947301
ENST00000451522TBC1D14chr46996029+ENST00000307358HTRA3chr48288288+2159307171183388
ENST00000451522TBC1D14chr46996029+ENST00000382512HTRA3chr48288288+165330717895292
ENST00000448507TBC1D14chr46996029+ENST00000307358HTRA3chr48288287+28931041791917612
ENST00000448507TBC1D14chr46996029+ENST00000382512HTRA3chr48288287+23871041791629516
ENST00000409757TBC1D14chr46996029+ENST00000307358HTRA3chr48288287+29381086581962634
ENST00000409757TBC1D14chr46996029+ENST00000382512HTRA3chr48288287+24321086581674538
ENST00000410031TBC1D14chr46996029+ENST00000307358HTRA3chr48288287+2211359421235397
ENST00000410031TBC1D14chr46996029+ENST00000382512HTRA3chr48288287+170535942947301
ENST00000451522TBC1D14chr46996029+ENST00000307358HTRA3chr48288287+2159307171183388
ENST00000451522TBC1D14chr46996029+ENST00000382512HTRA3chr48288287+165330717895292

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000448507ENST00000307358TBC1D14chr46925838+HTRA3chr48288288+0.0099788450.99002117
ENST00000448507ENST00000382512TBC1D14chr46925838+HTRA3chr48288288+0.0079471050.9920529
ENST00000409757ENST00000307358TBC1D14chr46925838+HTRA3chr48288288+0.0091849420.99081504
ENST00000409757ENST00000382512TBC1D14chr46925838+HTRA3chr48288288+0.0076212140.99237883
ENST00000448507ENST00000307358TBC1D14chr46996029+HTRA3chr48288288+0.0089897690.9910102
ENST00000448507ENST00000382512TBC1D14chr46996029+HTRA3chr48288288+0.0065563680.9934436
ENST00000409757ENST00000307358TBC1D14chr46996029+HTRA3chr48288288+0.0085277120.9914723
ENST00000409757ENST00000382512TBC1D14chr46996029+HTRA3chr48288288+0.0062213340.99377865
ENST00000410031ENST00000307358TBC1D14chr46996029+HTRA3chr48288288+0.0017584750.99824154
ENST00000410031ENST00000382512TBC1D14chr46996029+HTRA3chr48288288+0.002029350.99797064
ENST00000451522ENST00000307358TBC1D14chr46996029+HTRA3chr48288288+0.0035215460.9964785
ENST00000451522ENST00000382512TBC1D14chr46996029+HTRA3chr48288288+0.0044225820.99557745
ENST00000448507ENST00000307358TBC1D14chr46996029+HTRA3chr48288287+0.0089897690.9910102
ENST00000448507ENST00000382512TBC1D14chr46996029+HTRA3chr48288287+0.0065563680.9934436
ENST00000409757ENST00000307358TBC1D14chr46996029+HTRA3chr48288287+0.0085277120.9914723
ENST00000409757ENST00000382512TBC1D14chr46996029+HTRA3chr48288287+0.0062213340.99377865
ENST00000410031ENST00000307358TBC1D14chr46996029+HTRA3chr48288287+0.0017584750.99824154
ENST00000410031ENST00000382512TBC1D14chr46996029+HTRA3chr48288287+0.002029350.99797064
ENST00000451522ENST00000307358TBC1D14chr46996029+HTRA3chr48288287+0.0035215460.9964785
ENST00000451522ENST00000382512TBC1D14chr46996029+HTRA3chr48288287+0.0044225820.99557745

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for TBC1D14-HTRA3

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
TBC1D14chr46925838HTRA3chr48288288801240KLKILGPFSNFFARHPLFGRNVPLSS
TBC1D14chr46925838HTRA3chr48288288846262KLKILGPFSNFFARHPLFGRNVPLSS

Top

Potential FusionNeoAntigen Information of TBC1D14-HTRA3 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
TBC1D14-HTRA3_6925838_8288288.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
TBC1D14-HTRA3chr46925838chr48288288846HLA-B14:02SNFFARHPL0.98880.6941817
TBC1D14-HTRA3chr46925838chr48288288846HLA-B14:01SNFFARHPL0.98880.6941817
TBC1D14-HTRA3chr46925838chr48288288846HLA-A68:24FARHPLFGR0.98410.56261120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:01FARHPLFGR0.98280.56171120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:05FARHPLFGR0.98280.56171120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A68:03FARHPLFGR0.97940.56841120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A66:01FARHPLFGR0.94340.65751120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A68:08FARHPLFGR0.92590.61411120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A68:05FARHPLFGR0.91870.55921120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A34:05FARHPLFGR0.90830.57341120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A34:01FARHPLFGR0.90830.57341120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A31:02FARHPLFGR0.87290.7751120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A66:03FARHPLFGR0.79920.58711120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A31:06FARHPLFGR0.77440.72021120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:05FFARHPLFGR0.98170.60881020
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:01FFARHPLFGR0.98170.60881020
TBC1D14-HTRA3chr46925838chr48288288846HLA-A31:02FFARHPLFGR0.96250.76891020
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:05NFFARHPLFGR0.99090.5585920
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:01NFFARHPLFGR0.99090.5585920
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:67FFARHPLF0.99560.94411018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:80FFARHPLF0.99560.94411018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C04:14FFARHPLF0.96520.59951018
TBC1D14-HTRA3chr46925838chr48288288846HLA-A68:01FARHPLFGR0.98410.56261120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A33:03FARHPLFGR0.97130.59891120
TBC1D14-HTRA3chr46925838chr48288288846HLA-A31:01FARHPLFGR0.95090.76311120
TBC1D14-HTRA3chr46925838chr48288288846HLA-B15:21NFFARHPLF0.76770.8322918
TBC1D14-HTRA3chr46925838chr48288288846HLA-B14:03SNFFARHPL0.73840.6919817
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:05NFFARHPLF0.54190.9171918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:67NFFARHPLF0.53470.8323918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:80NFFARHPLF0.53470.8323918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:10NFFARHPLF0.51970.8628918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:27NFFARHPLF0.47240.8934918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:29NFFARHPLF0.43750.8677918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:46NFFARHPLF0.4290.6495918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:13NFFARHPLF0.39490.7777918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:02FFARHPLF0.99560.94411018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:17FFARHPLF0.99430.96751018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C06:06FFARHPLF0.97850.98411018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C04:04FFARHPLF0.97730.57991018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C14:03FFARHPLF0.97230.95341018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C14:02FFARHPLF0.97230.95341018
TBC1D14-HTRA3chr46925838chr48288288846HLA-C14:03NFFARHPL0.88360.9392917
TBC1D14-HTRA3chr46925838chr48288288846HLA-C14:02NFFARHPL0.88360.9392917
TBC1D14-HTRA3chr46925838chr48288288846HLA-A66:02FARHPLFGR0.81790.59031120
TBC1D14-HTRA3chr46925838chr48288288846HLA-C03:67NFFARHPLF0.58550.962918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:02NFFARHPLF0.53470.8323918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C07:17NFFARHPLF0.53460.9375918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C14:02NFFARHPLF0.17160.9269918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C14:03NFFARHPLF0.17160.9269918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C06:06NFFARHPLF0.07150.9715918
TBC1D14-HTRA3chr46925838chr48288288846HLA-C04:04NFFARHPLF0.03650.6334918

Top

Potential FusionNeoAntigen Information of TBC1D14-HTRA3 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
TBC1D14-HTRA3_6925838_8288288.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1501LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1501KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1501KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1504LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1505LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1505KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1505KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1506LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1506KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1506KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1507LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1509LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1509KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1509KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1512LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1513LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1513KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1513KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1516LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1516KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1516KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1518LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1518KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1520LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1520KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1520KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1521LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1522LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1522KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1522KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1524LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1524KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1524KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1528LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1528KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1528KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1532LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1532KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1532KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1533LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1533KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1533KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1535LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1536LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1536KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1536KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1537LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1540LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1540KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1540KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1541LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1541KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1541KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1542LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1542KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1542KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1543LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1543KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1543KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1545LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1545KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1545KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1546LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1546KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1546KILGPFSNFFARHPL217
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1548LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1549LKILGPFSNFFARHP116
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1549KLKILGPFSNFFARH015
TBC1D14-HTRA3chr46925838chr48288288846DRB1-1549KILGPFSNFFARHPL217

Top

Fusion breakpoint peptide structures of TBC1D14-HTRA3

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
6629PFSNFFARHPLFGRTBC1D14HTRA3chr46925838chr48288288846

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of TBC1D14-HTRA3

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN6629PFSNFFARHPLFGR-5.45197-6.48727
HLA-B14:023BVN6629PFSNFFARHPLFGR-4.14279-4.25619
HLA-B52:013W396629PFSNFFARHPLFGR-5.73729-5.85069
HLA-B52:013W396629PFSNFFARHPLFGR-4.5775-5.6128
HLA-A11:014UQ26629PFSNFFARHPLFGR-8.75853-8.87193
HLA-A24:025HGA6629PFSNFFARHPLFGR-6.32849-7.36379
HLA-A24:025HGA6629PFSNFFARHPLFGR-5.57943-5.69283
HLA-B27:056PYJ6629PFSNFFARHPLFGR-6.03529-6.14869
HLA-B27:056PYJ6629PFSNFFARHPLFGR-5.13869-6.17399
HLA-B44:053DX86629PFSNFFARHPLFGR-5.02027-5.13367
HLA-B44:053DX86629PFSNFFARHPLFGR-3.29286-4.32816
HLA-A02:016TDR6629PFSNFFARHPLFGR-6.56608-6.67948

Top

Vaccine Design for the FusionNeoAntigens of TBC1D14-HTRA3

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
TBC1D14-HTRA3chr46925838chr482882881018FFARHPLFCTTTGCAAGACACCCGCTGTTTGG
TBC1D14-HTRA3chr46925838chr482882881020FFARHPLFGRCTTTGCAAGACACCCGCTGTTTGGCCGCAA
TBC1D14-HTRA3chr46925838chr482882881120FARHPLFGRTGCAAGACACCCGCTGTTTGGCCGCAA
TBC1D14-HTRA3chr46925838chr48288288817SNFFARHPLTAACTTCTTTGCAAGACACCCGCTGTT
TBC1D14-HTRA3chr46925838chr48288288917NFFARHPLCTTCTTTGCAAGACACCCGCTGTT
TBC1D14-HTRA3chr46925838chr48288288918NFFARHPLFCTTCTTTGCAAGACACCCGCTGTTTGG
TBC1D14-HTRA3chr46925838chr48288288920NFFARHPLFGRCTTCTTTGCAAGACACCCGCTGTTTGGCCGCAA

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
TBC1D14-HTRA3chr46925838chr48288288015KLKILGPFSNFFARHATTGAAAATATTGGGGCCATTTAGTAACTTCTTTGCAAGACACCC
TBC1D14-HTRA3chr46925838chr48288288116LKILGPFSNFFARHPGAAAATATTGGGGCCATTTAGTAACTTCTTTGCAAGACACCCGCT
TBC1D14-HTRA3chr46925838chr48288288217KILGPFSNFFARHPLAATATTGGGGCCATTTAGTAACTTCTTTGCAAGACACCCGCTGTT

Top

Information of the samples that have these potential fusion neoantigens of TBC1D14-HTRA3

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
BLCATBC1D14-HTRA3chr46925838ENST00000409757chr48288288ENST00000307358TCGA-DK-A3IS-01A

Top

Potential target of CAR-T therapy development for TBC1D14-HTRA3

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

Top

Related Drugs to TBC1D14-HTRA3

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to TBC1D14-HTRA3

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource