FusionNeoAntigen Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Fusion Gene and Fusion Protein Summary

leaf

Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

leaf

Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

leaf

Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

leaf

Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

leaf

Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

leaf

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

leaf

Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

leaf

Potential target of CAR-T therapy development

leaf

Information on the samples that have these potential fusion neoantigens

leaf

Fusion Protein Targeting Drugs - (Manual Curation)

leaf

Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:UBXN6-GRM4

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: UBXN6-GRM4
FusionPDB ID: 96530
FusionGDB2.0 ID: 96530
HgeneTgene
Gene symbol

UBXN6

GRM4

Gene ID

80700

2914

Gene nameUBX domain protein 6glutamate metabotropic receptor 4
SynonymsUBXD1|UBXDC2GPRC1D|MGLUR4|mGlu4
Cytomap

19p13.3

6p21.31

Type of geneprotein-codingprotein-coding
DescriptionUBX domain-containing protein 6CTB-50L17.16UBX domain containing 1UBX domain-containing 2UBX domain-containing protein 1metabotropic glutamate receptor 4glutamate receptor, metabotropic 4
Modification date2020032720200313
UniProtAcc.

Q14833

Main function of 5'-partner protein: FUNCTION: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity. {ECO:0000269|PubMed:7617140, ECO:0000269|PubMed:8738157, ECO:0000269|PubMed:9473604}.
Ensembl transtripts involved in fusion geneENST idsENST00000301281, ENST00000394765, 
ENST00000455714, ENST00000535756, 
ENST00000545715, ENST00000609222, 
ENST00000374177, ENST00000374181, 
ENST00000538487, ENST00000544773, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score4 X 4 X 4=647 X 7 X 6=294
# samples 59
** MAII scorelog2(5/64*10)=-0.356143810225275
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(9/294*10)=-1.70781924850669
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: UBXN6 [Title/Abstract] AND GRM4 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: UBXN6 [Title/Abstract] AND GRM4 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)UBXN6(4452361)-GRM4(34008525), # samples:2
Anticipated loss of major functional domain due to fusion event.UBXN6-GRM4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
UBXN6-GRM4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
UBXN6-GRM4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
UBXN6-GRM4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
UBXN6-GRM4 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
UBXN6-GRM4 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
UBXN6-GRM4 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgeneGRM4

GO:0000187

activation of MAPK activity

15102938



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:4452361/chr6:34008525)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across UBXN6 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across GRM4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000394765UBXN6chr194452361-ENST00000538487GRM4chr634008525-29774363662006546
ENST00000394765UBXN6chr194452361-ENST00000374181GRM4chr634008525-29764363662006546
ENST00000394765UBXN6chr194452361-ENST00000374177GRM4chr634008525-29744363662006546
ENST00000394765UBXN6chr194452361-ENST00000544773GRM4chr634008525-23954363662006546
ENST00000394765UBXN6chr194452360-ENST00000538487GRM4chr634008525-29774363662006546
ENST00000394765UBXN6chr194452360-ENST00000374181GRM4chr634008525-29764363662006546
ENST00000394765UBXN6chr194452360-ENST00000374177GRM4chr634008525-29744363662006546
ENST00000394765UBXN6chr194452360-ENST00000544773GRM4chr634008525-23954363662006546

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000394765ENST00000538487UBXN6chr194452361-GRM4chr634008525-0.0182360860.9817639
ENST00000394765ENST00000374181UBXN6chr194452361-GRM4chr634008525-0.0180377590.98196226
ENST00000394765ENST00000374177UBXN6chr194452361-GRM4chr634008525-0.0177129230.9822871
ENST00000394765ENST00000544773UBXN6chr194452361-GRM4chr634008525-0.0277083460.9722917
ENST00000394765ENST00000538487UBXN6chr194452360-GRM4chr634008525-0.0182360860.9817639
ENST00000394765ENST00000374181UBXN6chr194452360-GRM4chr634008525-0.0180377590.98196226
ENST00000394765ENST00000374177UBXN6chr194452360-GRM4chr634008525-0.0177129230.9822871
ENST00000394765ENST00000544773UBXN6chr194452360-GRM4chr634008525-0.0277083460.9722917

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for UBXN6-GRM4

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
UBXN6chr194452360GRM4chr63400852543618GHPEEGPAGRLHQGGHSLDRERIGQD
UBXN6chr194452361GRM4chr63400852543618GHPEEGPAGRLHQGGHSLDRERIGQD

Top

Potential FusionNeoAntigen Information of UBXN6-GRM4 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
UBXN6-GRM4_4452360_34008525.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
UBXN6-GRM4chr194452360chr634008525436HLA-B39:01LHQGGHSL0.99760.98721018
UBXN6-GRM4chr194452360chr634008525436HLA-B39:24LHQGGHSL0.99750.84291018
UBXN6-GRM4chr194452360chr634008525436HLA-B38:02LHQGGHSL0.99570.99641018
UBXN6-GRM4chr194452360chr634008525436HLA-B38:01LHQGGHSL0.99480.99681018
UBXN6-GRM4chr194452360chr634008525436HLA-B15:10LHQGGHSL0.98520.73381018
UBXN6-GRM4chr194452360chr634008525436HLA-B15:37LHQGGHSL0.97760.76511018
UBXN6-GRM4chr194452360chr634008525436HLA-B14:01LHQGGHSL0.97420.95551018
UBXN6-GRM4chr194452360chr634008525436HLA-B14:02LHQGGHSL0.97420.95551018
UBXN6-GRM4chr194452360chr634008525436HLA-B15:18LHQGGHSL0.90450.90851018
UBXN6-GRM4chr194452360chr634008525436HLA-B07:10LHQGGHSL0.54270.56071018
UBXN6-GRM4chr194452360chr634008525436HLA-B07:10RLHQGGHSL0.99540.6168918
UBXN6-GRM4chr194452360chr634008525436HLA-B07:02RLHQGGHSL0.99480.567918
UBXN6-GRM4chr194452360chr634008525436HLA-B07:05RLHQGGHSL0.99480.6753918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:01RLHQGGHSL0.99470.9575918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:22RLHQGGHSL0.97760.6862918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:13RLHQGGHSL0.95970.8427918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:27RLHQGGHSL0.93450.7167918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:38RLHQGGHSL0.93230.8208918
UBXN6-GRM4chr194452360chr634008525436HLA-A30:08RLHQGGHSL0.91090.9075918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:25RLHQGGHSL0.8610.9695918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:11RLHQGGHSL0.84680.6051918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:60RLHQGGHSL0.83530.56918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:24RLHQGGHSL0.8340.5788918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:67RLHQGGHSL0.8340.5788918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:30RLHQGGHSL0.8340.5788918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:04RLHQGGHSL0.82160.7423918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:17RLHQGGHSL0.81670.7024918
UBXN6-GRM4chr194452360chr634008525436HLA-B08:01RLHQGGHSL0.78270.9157918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:21RLHQGGHSL0.77510.6926918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:03RLHQGGHSL0.76010.9023918
UBXN6-GRM4chr194452360chr634008525436HLA-A32:13RLHQGGHSL0.74420.9759918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:35RLHQGGHSL0.73160.6046918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:19RLHQGGHSL0.69630.6588918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:29RLHQGGHSL0.66120.5871918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:20RLHQGGHSL0.63910.5882918
UBXN6-GRM4chr194452360chr634008525436HLA-B81:01RLHQGGHSL0.5260.8467918
UBXN6-GRM4chr194452360chr634008525436HLA-B48:01RLHQGGHSL0.47490.8878918
UBXN6-GRM4chr194452360chr634008525436HLA-B39:13RLHQGGHSL0.12470.9791918
UBXN6-GRM4chr194452360chr634008525436HLA-B13:01RLHQGGHSL0.08410.9939918
UBXN6-GRM4chr194452360chr634008525436HLA-B13:02RLHQGGHSL0.06070.8369918
UBXN6-GRM4chr194452360chr634008525436HLA-B27:05GRLHQGGHSL0.99990.6954818
UBXN6-GRM4chr194452360chr634008525436HLA-B27:04GRLHQGGHSL0.99990.8724818
UBXN6-GRM4chr194452360chr634008525436HLA-B27:07GRLHQGGHSL0.99980.7406818
UBXN6-GRM4chr194452360chr634008525436HLA-B39:24GRLHQGGHSL0.99430.8776818
UBXN6-GRM4chr194452360chr634008525436HLA-B07:10GRLHQGGHSL0.8440.7901818
UBXN6-GRM4chr194452360chr634008525436HLA-A74:11RLHQGGHSLDR0.99310.653920
UBXN6-GRM4chr194452360chr634008525436HLA-A74:09RLHQGGHSLDR0.99310.653920
UBXN6-GRM4chr194452360chr634008525436HLA-A74:03RLHQGGHSLDR0.99310.653920
UBXN6-GRM4chr194452360chr634008525436HLA-B39:09LHQGGHSL0.9980.94581018
UBXN6-GRM4chr194452360chr634008525436HLA-B39:12LHQGGHSL0.99630.98781018
UBXN6-GRM4chr194452360chr634008525436HLA-B39:05LHQGGHSL0.99440.98371018
UBXN6-GRM4chr194452360chr634008525436HLA-B14:03LHQGGHSL0.82770.94761018
UBXN6-GRM4chr194452360chr634008525436HLA-B07:12RLHQGGHSL0.98050.7631918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:02RLHQGGHSL0.97860.5083918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:05RLHQGGHSL0.96770.6457918
UBXN6-GRM4chr194452360chr634008525436HLA-B07:04RLHQGGHSL0.96680.6178918
UBXN6-GRM4chr194452360chr634008525436HLA-B14:03RLHQGGHSL0.96290.9452918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:04RLHQGGHSL0.96250.9723918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:07RLHQGGHSL0.94730.9198918
UBXN6-GRM4chr194452360chr634008525436HLA-B42:02RLHQGGHSL0.88090.9232918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:01RLHQGGHSL0.8340.5788918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:19RLHQGGHSL0.82290.9944918
UBXN6-GRM4chr194452360chr634008525436HLA-C07:29RLHQGGHSL0.8130.9683918
UBXN6-GRM4chr194452360chr634008525436HLA-C01:17RLHQGGHSL0.81090.9856918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:08RLHQGGHSL0.76110.9382918
UBXN6-GRM4chr194452360chr634008525436HLA-C01:30RLHQGGHSL0.5770.9854918
UBXN6-GRM4chr194452360chr634008525436HLA-B39:08RLHQGGHSL0.57040.9824918
UBXN6-GRM4chr194452360chr634008525436HLA-B39:09RLHQGGHSL0.32260.9099918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:05RLHQGGHSL0.15040.948918
UBXN6-GRM4chr194452360chr634008525436HLA-B48:03RLHQGGHSL0.07560.821918
UBXN6-GRM4chr194452360chr634008525436HLA-B27:14GRLHQGGHSL0.99990.7401818
UBXN6-GRM4chr194452360chr634008525436HLA-B27:03GRLHQGGHSL0.99530.7238818
UBXN6-GRM4chr194452360chr634008525436HLA-B39:12GRLHQGGHSL0.98310.9526818
UBXN6-GRM4chr194452360chr634008525436HLA-B39:31LHQGGHSL0.9970.98751018
UBXN6-GRM4chr194452360chr634008525436HLA-B38:05LHQGGHSL0.99480.99681018
UBXN6-GRM4chr194452360chr634008525436HLA-B15:09LHQGGHSL0.97970.94471018
UBXN6-GRM4chr194452360chr634008525436HLA-B39:11LHQGGHSL0.91950.97511018
UBXN6-GRM4chr194452360chr634008525436HLA-B07:13RLHQGGHSL0.99610.9061918
UBXN6-GRM4chr194452360chr634008525436HLA-B07:09RLHQGGHSL0.99520.6099918
UBXN6-GRM4chr194452360chr634008525436HLA-B48:05RLHQGGHSL0.9950.6109918
UBXN6-GRM4chr194452360chr634008525436HLA-B07:22RLHQGGHSL0.99480.567918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:135RLHQGGHSL0.99480.9629918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:125RLHQGGHSL0.99470.9575918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:33RLHQGGHSL0.99470.9575918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:34RLHQGGHSL0.99470.9575918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:24RLHQGGHSL0.99470.9865918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:27RLHQGGHSL0.99440.9757918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:03RLHQGGHSL0.98550.7445918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:68RLHQGGHSL0.96890.8693918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:73RLHQGGHSL0.96680.9837918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:50RLHQGGHSL0.96370.9742918
UBXN6-GRM4chr194452360chr634008525436HLA-A32:01RLHQGGHSL0.96170.984918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:53RLHQGGHSL0.96010.9554918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:12RLHQGGHSL0.95610.9494918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:35RLHQGGHSL0.94990.9706918
UBXN6-GRM4chr194452360chr634008525436HLA-C07:04RLHQGGHSL0.90620.9875918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:54RLHQGGHSL0.90490.9386918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:30RLHQGGHSL0.88490.9844918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:39RLHQGGHSL0.87950.9364918
UBXN6-GRM4chr194452360chr634008525436HLA-B27:06RLHQGGHSL0.8320.8315918
UBXN6-GRM4chr194452360chr634008525436HLA-C01:02RLHQGGHSL0.79340.9847918
UBXN6-GRM4chr194452360chr634008525436HLA-B08:18RLHQGGHSL0.78270.9157918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:14RLHQGGHSL0.78250.6469918
UBXN6-GRM4chr194452360chr634008525436HLA-B08:12RLHQGGHSL0.77960.9593918
UBXN6-GRM4chr194452360chr634008525436HLA-A02:06RLHQGGHSL0.77510.6926918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:03RLHQGGHSL0.75280.9918918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:04RLHQGGHSL0.75280.9918918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:05RLHQGGHSL0.74620.9422918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:17RLHQGGHSL0.73980.987918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:67RLHQGGHSL0.72670.9869918
UBXN6-GRM4chr194452360chr634008525436HLA-C01:03RLHQGGHSL0.69060.9794918
UBXN6-GRM4chr194452360chr634008525436HLA-C03:06RLHQGGHSL0.60790.9937918
UBXN6-GRM4chr194452360chr634008525436HLA-C17:01RLHQGGHSL0.5450.9901918
UBXN6-GRM4chr194452360chr634008525436HLA-B39:11RLHQGGHSL0.49870.967918
UBXN6-GRM4chr194452360chr634008525436HLA-B39:02RLHQGGHSL0.25560.9786918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:20RLHQGGHSL0.17410.9656918
UBXN6-GRM4chr194452360chr634008525436HLA-B35:13RLHQGGHSL0.16670.9657918
UBXN6-GRM4chr194452360chr634008525436HLA-B35:28RLHQGGHSL0.15330.9675918
UBXN6-GRM4chr194452360chr634008525436HLA-B15:09RLHQGGHSL0.11390.9257918
UBXN6-GRM4chr194452360chr634008525436HLA-B40:12RLHQGGHSL0.07560.821918
UBXN6-GRM4chr194452360chr634008525436HLA-B40:21RLHQGGHSL0.05560.8768918
UBXN6-GRM4chr194452360chr634008525436HLA-B40:49RLHQGGHSL0.02540.7685918
UBXN6-GRM4chr194452360chr634008525436HLA-B27:10GRLHQGGHSL0.99990.9201818
UBXN6-GRM4chr194452360chr634008525436HLA-B27:08GRLHQGGHSL0.99990.6423818
UBXN6-GRM4chr194452360chr634008525436HLA-B27:09GRLHQGGHSL0.99980.6882818
UBXN6-GRM4chr194452360chr634008525436HLA-B27:06GRLHQGGHSL0.99980.9042818
UBXN6-GRM4chr194452360chr634008525436HLA-B39:31GRLHQGGHSL0.96060.9509818
UBXN6-GRM4chr194452360chr634008525436HLA-B15:73GRLHQGGHSL0.72870.9759818
UBXN6-GRM4chr194452360chr634008525436HLA-B15:30GRLHQGGHSL0.69650.9797818
UBXN6-GRM4chr194452360chr634008525436HLA-B40:21GRLHQGGHSL0.45230.9262818
UBXN6-GRM4chr194452360chr634008525436HLA-A74:01RLHQGGHSLDR0.99310.653920
UBXN6-GRM4chr194452360chr634008525436HLA-B15:30AGRLHQGGHSL0.97360.9732718

Top

Potential FusionNeoAntigen Information of UBXN6-GRM4 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

Top

Fusion breakpoint peptide structures of UBXN6-GRM4

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
6484PAGRLHQGGHSLDRUBXN6GRM4chr194452360chr634008525436

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of UBXN6-GRM4

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN6484PAGRLHQGGHSLDR-7.9962-8.1096
HLA-B14:023BVN6484PAGRLHQGGHSLDR-5.70842-6.74372
HLA-B52:013W396484PAGRLHQGGHSLDR-6.83737-6.95077
HLA-B52:013W396484PAGRLHQGGHSLDR-4.4836-5.5189
HLA-A11:014UQ26484PAGRLHQGGHSLDR-10.0067-10.1201
HLA-A11:014UQ26484PAGRLHQGGHSLDR-9.03915-10.0745
HLA-A24:025HGA6484PAGRLHQGGHSLDR-6.56204-6.67544
HLA-A24:025HGA6484PAGRLHQGGHSLDR-5.42271-6.45801
HLA-B44:053DX86484PAGRLHQGGHSLDR-7.85648-8.89178
HLA-B44:053DX86484PAGRLHQGGHSLDR-5.3978-5.5112
HLA-A02:016TDR6484PAGRLHQGGHSLDR-3.37154-4.40684

Top

Vaccine Design for the FusionNeoAntigens of UBXN6-GRM4

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
UBXN6-GRM4chr194452360chr6340085251018LHQGGHSLATTCTCTTGACCGTGAGCGAATTG
UBXN6-GRM4chr194452360chr634008525718AGRLHQGGHSLAAGGAGGCCATTCTCTTGACCGTGAGCGAATTG
UBXN6-GRM4chr194452360chr634008525818GRLHQGGHSLGAGGCCATTCTCTTGACCGTGAGCGAATTG
UBXN6-GRM4chr194452360chr634008525918RLHQGGHSLGCCATTCTCTTGACCGTGAGCGAATTG
UBXN6-GRM4chr194452360chr634008525920RLHQGGHSLDRGCCATTCTCTTGACCGTGAGCGAATTGGGCAGG

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

Top

Information of the samples that have these potential fusion neoantigens of UBXN6-GRM4

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
OVUBXN6-GRM4chr194452360ENST00000394765chr634008525ENST00000374177TCGA-29-1783

Top

Potential target of CAR-T therapy development for UBXN6-GRM4

check button Predicted 3D structure. We used RoseTTAFold.
515_UBXN6-GRM4_490bb_pred.pdb


check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739588_6100797.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739625_6450797.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739657_6750797.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739700_7200797.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739751_7720797.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739786_8080797.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452360chr6:34008525ENST0000037417739822_8470797.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410588_6100913.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410625_6450913.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410657_6750913.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410700_7200913.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410751_7720913.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410786_8080913.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452360chr6:34008525ENST00000374181410822_8470913.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410588_6100773.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410625_6450773.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410657_6750773.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410700_7200773.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410751_7720773.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410786_8080773.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452360chr6:34008525ENST00000455714410822_8470773.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410588_6100780.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410625_6450780.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410657_6750780.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410700_7200780.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410751_7720780.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410786_8080780.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452360chr6:34008525ENST00000535756410822_8470780.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511588_6100913.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511625_6450913.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511657_6750913.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511700_7200913.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511751_7720913.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511786_8080913.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452360chr6:34008525ENST00000538487511822_8470913.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410588_6100744.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410625_6450744.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410657_6750744.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410700_7200744.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410751_7720744.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410786_8080744.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452360chr6:34008525ENST00000544773410822_8470744.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739588_6100797.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739625_6450797.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739657_6750797.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739700_7200797.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739751_7720797.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739786_8080797.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452361chr6:34008525ENST0000037417739822_8470797.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410588_6100913.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410625_6450913.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410657_6750913.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410700_7200913.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410751_7720913.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410786_8080913.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452361chr6:34008525ENST00000374181410822_8470913.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410588_6100773.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410625_6450773.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410657_6750773.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410700_7200773.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410751_7720773.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410786_8080773.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452361chr6:34008525ENST00000455714410822_8470773.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410588_6100780.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410625_6450780.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410657_6750780.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410700_7200780.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410751_7720780.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410786_8080780.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452361chr6:34008525ENST00000535756410822_8470780.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511588_6100913.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511625_6450913.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511657_6750913.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511700_7200913.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511751_7720913.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511786_8080913.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452361chr6:34008525ENST00000538487511822_8470913.0TransmembraneHelical%3B Name%3D7
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410588_6100744.0TransmembraneHelical%3B Name%3D1
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410625_6450744.0TransmembraneHelical%3B Name%3D2
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410657_6750744.0TransmembraneHelical%3B Name%3D3
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410700_7200744.0TransmembraneHelical%3B Name%3D4
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410751_7720744.0TransmembraneHelical%3B Name%3D5
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410786_8080744.0TransmembraneHelical%3B Name%3D6
TgeneGRM4chr19:4452361chr6:34008525ENST00000544773410822_8470744.0TransmembraneHelical%3B Name%3D7

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result
UBXN6chr194452360ENST00000394765GRM4chr634008525ENST00000374177

Top

Related Drugs to UBXN6-GRM4

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to UBXN6-GRM4

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource