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Fusion Protein:VAC14-WWOX

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: VAC14-WWOX
	FusionPDB ID: 97842
	FusionGDB2.0 ID: 97842
		Hgene	Tgene
	Gene symbol	VAC14	WWOX
	Gene ID	55697	51741
	Gene name	VAC14 component of PIKFYVE complex	WW domain containing oxidoreductase
	Synonyms	ArPIKfyve\|TAX1BP2\|TRX	D16S432E\|EIEE28\|FOR\|FRA16D\|HHCMA56\|PRO0128\|SCAR12\|SDR41C1\|WOX1
	Cytomap	16q22.1-q22.2	16q23.1-q23.2
	Type of gene	protein-coding	protein-coding
	Description	protein VAC14 homologTax1 (human T-cell leukemia virus type I) binding proteinVac14, PIKFYVE complex component	WW domain-containing oxidoreductaseWW domain-containing protein WWOXfragile site FRA16D oxidoreductaseshort chain dehydrogenase/reductase family 41C member 1
	Modification date	20200327	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000261776, ENST00000536184, ENST00000571759,	ENST00000355860, ENST00000408984, ENST00000565791, ENST00000569818, ENST00000402655, ENST00000406884, ENST00000539474, ENST00000566780,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	5 X 6 X 4=120	30 X 23 X 10=6900
	# samples	6	36
	** MAII score	log2(6/120*10)=-1 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(36/6900*10)=-4.26052755022322 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: VAC14 [Title/Abstract] AND WWOX [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: VAC14 [Title/Abstract] AND WWOX [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	VAC14(70765398)-WWOX(79245505), # samples:3
Anticipated loss of major functional domain due to fusion event.	VAC14-WWOX seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. VAC14-WWOX seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. VAC14-WWOX seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. VAC14-WWOX seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. VAC14-WWOX seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. VAC14-WWOX seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. VAC14-WWOX seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. VAC14-WWOX seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	WWOX	GO:0030178	negative regulation of Wnt signaling pathway	19465938
Tgene	WWOX	GO:0071560	cellular response to transforming growth factor beta stimulus	19366691

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:70765398/chr16:79245505)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across VAC14 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across WWOX (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000261776	VAC14	chr16	70765398	-	ENST00000566780	WWOX	chr16	79245505	+	2982	1922	261	2078	605
ENST00000261776	VAC14	chr16	70765398	-	ENST00000406884	WWOX	chr16	79245505	+	2982	1922	261	2078	605
ENST00000261776	VAC14	chr16	70765397	-	ENST00000566780	WWOX	chr16	79245504	+	2982	1922	261	2078	605
ENST00000261776	VAC14	chr16	70765397	-	ENST00000406884	WWOX	chr16	79245504	+	2982	1922	261	2078	605
ENST00000261776	VAC14	chr16	70732540	-	ENST00000539474	WWOX	chr16	79245505	+	3157	2097	261	2285	674
ENST00000536184	VAC14	chr16	70732540	-	ENST00000539474	WWOX	chr16	79245505	+	2222	1162	900	1688	262

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000261776	ENST00000566780	VAC14	chr16	70765398	-	WWOX	chr16	79245505	+	0.005276051	0.994724
ENST00000261776	ENST00000406884	VAC14	chr16	70765398	-	WWOX	chr16	79245505	+	0.005276051	0.994724
ENST00000261776	ENST00000566780	VAC14	chr16	70765397	-	WWOX	chr16	79245504	+	0.005276051	0.994724
ENST00000261776	ENST00000406884	VAC14	chr16	70765397	-	WWOX	chr16	79245504	+	0.005276051	0.994724
ENST00000261776	ENST00000539474	VAC14	chr16	70732540	-	WWOX	chr16	79245505	+	0.006206575	0.99379337
ENST00000536184	ENST00000539474	VAC14	chr16	70732540	-	WWOX	chr16	79245505	+	0.7861628	0.21383719

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for VAC14-WWOX

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
VAC14	chr16	70732540	WWOX	chr16	79245505	1162	86	ALPAKEPAEGPEDPATGSCHHRVLCC
VAC14	chr16	70732540	WWOX	chr16	79245505	2097	612	FQLRNQLKDLKTLQQGAATTVYCAAV
VAC14	chr16	70765397	WWOX	chr16	79245504	1922	551	SSERKLLEVRGPFIISNRELPPPCTV
VAC14	chr16	70765398	WWOX	chr16	79245505	1922	551	SSERKLLEVRGPFIISNRELPPPCTV

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Potential FusionNeoAntigen Information of VAC14-WWOX in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

VAC14-WWOX_70732540_79245505.msa
VAC14-WWOX_70765397_79245504.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
VAC14-WWOX	chr16	70732540	chr16	79245505	1162	HLA-B45:01	AEGPEDPAT	0.9519	0.9259	7	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B08:09	DLKTLQQGA	0.8031	0.9382	8	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B13:02	LQQGAATTV	0.7757	0.9884	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-A02:21	LQQGAATTV	0.7428	0.8906	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B13:01	LQQGAATTV	0.4773	0.9985	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B39:13	LQQGAATTV	0.3152	0.9899	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B52:01	LQQGAATTV	0.0445	0.9925	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:01	LQQGAATTVY	0.9999	0.8546	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:25	LQQGAATTVY	0.9826	0.957	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:02	LQQGAATTVY	0.9606	0.9674	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:03	LQQGAATTVY	0.7972	0.8904	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:01	TLQQGAATTVY	0.9998	0.8671	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:25	TLQQGAATTVY	0.9912	0.952	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:02	TLQQGAATTVY	0.9872	0.96	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B13:02	KTLQQGAATTV	0.6829	0.9874	10	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B48:03	LQQGAATTV	0.4607	0.9697	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:07	LQQGAATTVY	0.9988	0.7017	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:04	LQQGAATTVY	0.9903	0.8908	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:21	LQQGAATTVY	0.9648	0.9447	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:05	LQQGAATTVY	0.884	0.9087	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:31	LQQGAATTVY	0.8108	0.91	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:05	TLQQGAATTVY	0.9533	0.9004	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:31	TLQQGAATTVY	0.9296	0.9032	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:73	LQQGAATTV	0.8746	0.9911	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-A02:06	LQQGAATTV	0.7428	0.8906	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:30	LQQGAATTV	0.737	0.9856	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B49:03	LQQGAATTV	0.494	0.5057	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B40:12	LQQGAATTV	0.4607	0.9697	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B39:02	LQQGAATTV	0.4235	0.9902	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B40:21	LQQGAATTV	0.307	0.9743	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B48:05	LQQGAATTV	0.1852	0.5628	12	21
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:125	LQQGAATTVY	0.9999	0.8546	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:27	LQQGAATTVY	0.9999	0.8422	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:34	LQQGAATTVY	0.9999	0.8546	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:33	LQQGAATTVY	0.9999	0.8546	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:135	LQQGAATTVY	0.9998	0.8594	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:50	LQQGAATTVY	0.9997	0.8701	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:24	LQQGAATTVY	0.9996	0.8988	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:35	LQQGAATTVY	0.9982	0.7444	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:53	LQQGAATTVY	0.9977	0.8191	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:12	LQQGAATTVY	0.9963	0.8653	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:54	LQQGAATTVY	0.9925	0.8129	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:39	LQQGAATTVY	0.9824	0.9135	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:68	LQQGAATTVY	0.964	0.6632	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:20	LQQGAATTVY	0.8842	0.9441	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B35:28	LQQGAATTVY	0.8677	0.9529	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B35:20	LQQGAATTVY	0.7911	0.9559	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B48:02	LQQGAATTVY	0.6526	0.9461	12	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:135	TLQQGAATTVY	0.9998	0.8758	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:34	TLQQGAATTVY	0.9998	0.8671	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:27	TLQQGAATTVY	0.9998	0.8372	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:33	TLQQGAATTVY	0.9998	0.8671	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:50	TLQQGAATTVY	0.9998	0.8502	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:125	TLQQGAATTVY	0.9998	0.8671	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:12	TLQQGAATTVY	0.9992	0.889	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:39	TLQQGAATTVY	0.9919	0.9104	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B15:20	TLQQGAATTVY	0.9548	0.9348	11	22
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	HLA-B35:28	TLQQGAATTVY	0.9395	0.9519	11	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-B27:05	VRGPFIISNR	0.9995	0.8553	8	18
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:08	FIISNREL	0.9998	0.8401	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:07	FIISNREL	0.9997	0.9747	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:19	FIISNREL	0.9997	0.9835	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:03	FIISNREL	0.9999	0.9834	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:04	FIISNREL	0.9999	0.9834	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:17	FIISNREL	0.9997	0.946	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:05	FIISNREL	0.9995	0.8949	12	20
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	HLA-C03:67	FIISNREL	0.9995	0.9584	12	20

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Potential FusionNeoAntigen Information of VAC14-WWOX in HLA II

VAC14-WWOX_70732540_79245505.msa
VAC14-WWOX_70765397_79245504.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0403	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0403	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0413	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0413	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0415	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0415	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0415	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0415	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0419	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0427	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0427	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0436	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0436	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0436	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0436	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0437	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0437	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0437	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0437	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0439	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0439	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0440	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0440	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0440	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0441	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0441	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0442	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0442	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0442	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0442	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0443	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0443	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0444	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0444	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0446	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0446	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0447	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0447	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0449	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0449	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0450	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0450	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0451	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0451	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0452	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0452	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0453	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0453	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0453	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0453	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0454	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0455	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0455	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0456	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0456	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0456	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0456	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0458	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0458	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0458	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0458	NQLKDLKTLQQGAAT	4	19
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0459	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0459	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0459	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0460	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0460	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0461	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0465	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0465	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0467	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0468	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0468	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0468	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0470	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0470	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0470	QLRNQLKDLKTLQQG	1	16
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0471	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0471	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0473	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0473	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0475	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0478	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0479	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0479	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0485	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0485	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0488	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0488	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0804	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0828	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0831	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-0831	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1130	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1167	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1183	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1204	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1204	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1209	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1209	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1220	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1415	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1452	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1457	RNQLKDLKTLQQGAA	3	18
VAC14-WWOX	chr16	70732540	chr16	79245505	2097	DRB1-1457	LRNQLKDLKTLQQGA	2	17
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0466	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0802	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0805	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0808	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0809	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0811	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0821	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0824	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0829	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-0840	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1101	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1108	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1109	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1110	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1111	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1112	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1115	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1119	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1124	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1127	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1128	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1129	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1131	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1133	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1137	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1137	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1139	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1149	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1151	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1153	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1153	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1153	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1161	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1162	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1164	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1166	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1169	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1172	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1173	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1173	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1173	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1174	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1175	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1179	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1179	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1180	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1180	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1181	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1182	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1187	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1187	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1190	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1191	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1193	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1193	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1193	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1194	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1195	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1196	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1303	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1305	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1307	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1307	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-13100	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-13101	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-13101	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-13101	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1310	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1310	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1310	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1312	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1313	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1314	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1321	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1326	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1330	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1333	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1333	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1333	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1336	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1337	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1337	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1337	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1338	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1341	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1346	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1347	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1349	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1350	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1355	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1356	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1360	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1360	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1362	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1363	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1365	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1366	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1366	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1366	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1382	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1385	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1385	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1385	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1386	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1388	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1390	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1395	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1402	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1403	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1407	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1407	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1407	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1409	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1409	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1413	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1414	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1414	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1414	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1419	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1422	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1424	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1425	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1427	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1430	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1436	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1436	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1436	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1440	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1441	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1442	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1442	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1442	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1444	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1444	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1444	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1447	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1449	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1451	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1451	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1451	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1453	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1463	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1468	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1468	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1468	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1469	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1469	EVRGPFIISNRELPP	7	22
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1469	RGPFIISNRELPPPC	9	24
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1477	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1485	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1489	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1493	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1494	VRGPFIISNRELPPP	8	23
VAC14-WWOX	chr16	70765397	chr16	79245504	1922	DRB1-1498	VRGPFIISNRELPPP	8	23

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Fusion breakpoint peptide structures of VAC14-WWOX

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
4946	LEVRGPFIISNREL	VAC14	WWOX	chr16	70765397	chr16	79245504	1922

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
5145	LKDLKTLQQGAATT	VAC14	WWOX	chr16	70732540	chr16	79245505	2097

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
6478	PAEGPEDPATGSCH	VAC14	WWOX	chr16	70732540	chr16	79245505	1162

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of VAC14-WWOX

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	4946	LEVRGPFIISNREL	-7.9962	-8.1096
HLA-B14:02	3BVN	4946	LEVRGPFIISNREL	-5.70842	-6.74372
HLA-B52:01	3W39	4946	LEVRGPFIISNREL	-6.83737	-6.95077
HLA-B52:01	3W39	4946	LEVRGPFIISNREL	-4.4836	-5.5189
HLA-A11:01	4UQ2	4946	LEVRGPFIISNREL	-10.0067	-10.1201
HLA-A11:01	4UQ2	4946	LEVRGPFIISNREL	-9.03915	-10.0745
HLA-A24:02	5HGA	4946	LEVRGPFIISNREL	-6.56204	-6.67544
HLA-A24:02	5HGA	4946	LEVRGPFIISNREL	-5.42271	-6.45801
HLA-B44:05	3DX8	4946	LEVRGPFIISNREL	-7.85648	-8.89178
HLA-B44:05	3DX8	4946	LEVRGPFIISNREL	-5.3978	-5.5112
HLA-A02:01	6TDR	4946	LEVRGPFIISNREL	-3.37154	-4.40684
HLA-B14:02	3BVN	5145	LKDLKTLQQGAATT	-7.9962	-8.1096
HLA-B14:02	3BVN	5145	LKDLKTLQQGAATT	-5.70842	-6.74372
HLA-B52:01	3W39	5145	LKDLKTLQQGAATT	-6.83737	-6.95077
HLA-B52:01	3W39	5145	LKDLKTLQQGAATT	-4.4836	-5.5189
HLA-A11:01	4UQ2	5145	LKDLKTLQQGAATT	-10.0067	-10.1201
HLA-A11:01	4UQ2	5145	LKDLKTLQQGAATT	-9.03915	-10.0745
HLA-A24:02	5HGA	5145	LKDLKTLQQGAATT	-6.56204	-6.67544
HLA-A24:02	5HGA	5145	LKDLKTLQQGAATT	-5.42271	-6.45801
HLA-B44:05	3DX8	5145	LKDLKTLQQGAATT	-7.85648	-8.89178
HLA-B44:05	3DX8	5145	LKDLKTLQQGAATT	-5.3978	-5.5112
HLA-A02:01	6TDR	5145	LKDLKTLQQGAATT	-3.37154	-4.40684
HLA-B14:02	3BVN	6478	PAEGPEDPATGSCH	-7.9962	-8.1096
HLA-B14:02	3BVN	6478	PAEGPEDPATGSCH	-5.70842	-6.74372
HLA-B52:01	3W39	6478	PAEGPEDPATGSCH	-6.83737	-6.95077
HLA-B52:01	3W39	6478	PAEGPEDPATGSCH	-4.4836	-5.5189
HLA-A11:01	4UQ2	6478	PAEGPEDPATGSCH	-10.0067	-10.1201
HLA-A11:01	4UQ2	6478	PAEGPEDPATGSCH	-9.03915	-10.0745
HLA-A24:02	5HGA	6478	PAEGPEDPATGSCH	-6.56204	-6.67544
HLA-A24:02	5HGA	6478	PAEGPEDPATGSCH	-5.42271	-6.45801
HLA-B44:05	3DX8	6478	PAEGPEDPATGSCH	-7.85648	-8.89178
HLA-B44:05	3DX8	6478	PAEGPEDPATGSCH	-5.3978	-5.5112
HLA-A02:01	6TDR	6478	PAEGPEDPATGSCH	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of VAC14-WWOX

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
VAC14-WWOX	chr16	70732540	chr16	79245505	10	21	KTLQQGAATTV	AAGACCCTGCAACAGGGAGCTGCCACCACCGTG
VAC14-WWOX	chr16	70732540	chr16	79245505	11	22	TLQQGAATTVY	ACCCTGCAACAGGGAGCTGCCACCACCGTGTAC
VAC14-WWOX	chr16	70732540	chr16	79245505	12	21	LQQGAATTV	CTGCAACAGGGAGCTGCCACCACCGTG
VAC14-WWOX	chr16	70732540	chr16	79245505	12	22	LQQGAATTVY	CTGCAACAGGGAGCTGCCACCACCGTGTAC
VAC14-WWOX	chr16	70732540	chr16	79245505	7	16	AEGPEDPAT	AAGGACCTGAAGACCCTGCAACAGGGA
VAC14-WWOX	chr16	70732540	chr16	79245505	8	17	DLKTLQQGA	GACCTGAAGACCCTGCAACAGGGAGCT
VAC14-WWOX	chr16	70765397	chr16	79245504	12	20	FIISNREL	CAGCAACAGGGAGCTGCCACCACC
VAC14-WWOX	chr16	70765397	chr16	79245504	8	18	VRGPFIISNR	CCCTTTCATCATCAGCAACAGGGAGCTGCC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
VAC14-WWOX	chr16	70732540	chr16	79245505	1	16	QLRNQLKDLKTLQQG	CAGCTAAGGAACCAGCTGAAGGACCTGAAGACCCTGCAACAGGGA
VAC14-WWOX	chr16	70732540	chr16	79245505	2	17	LRNQLKDLKTLQQGA	CTAAGGAACCAGCTGAAGGACCTGAAGACCCTGCAACAGGGAGCT
VAC14-WWOX	chr16	70732540	chr16	79245505	3	18	RNQLKDLKTLQQGAA	AGGAACCAGCTGAAGGACCTGAAGACCCTGCAACAGGGAGCTGCC
VAC14-WWOX	chr16	70732540	chr16	79245505	4	19	NQLKDLKTLQQGAAT	AACCAGCTGAAGGACCTGAAGACCCTGCAACAGGGAGCTGCCACC
VAC14-WWOX	chr16	70765397	chr16	79245504	7	22	EVRGPFIISNRELPP	AGGCCCTTTCATCATCAGCAACAGGGAGCTGCCACCACCGTGTAC
VAC14-WWOX	chr16	70765397	chr16	79245504	8	23	VRGPFIISNRELPPP	CCCTTTCATCATCAGCAACAGGGAGCTGCCACCACCGTGTACTGT
VAC14-WWOX	chr16	70765397	chr16	79245504	9	24	RGPFIISNRELPPPC	TTTCATCATCAGCAACAGGGAGCTGCCACCACCGTGTACTGTGCT

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Information of the samples that have these potential fusion neoantigens of VAC14-WWOX

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BLCA	VAC14-WWOX	chr16	70765397	ENST00000261776	chr16	79245504	ENST00000406884	TCGA-BT-A20V
BLCA	VAC14-WWOX	chr16	70732540	ENST00000261776	chr16	79245505	ENST00000539474	TCGA-BT-A20V-01A
BLCA	VAC14-WWOX	chr16	70732540	ENST00000536184	chr16	79245505	ENST00000539474	TCGA-BT-A20V-01A

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Potential target of CAR-T therapy development for VAC14-WWOX

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to VAC14-WWOX

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to VAC14-WWOX

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)