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Fusion Protein:WDR76-IDH3A |
Fusion Gene and Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: WDR76-IDH3A | FusionPDB ID: 99094 | FusionGDB2.0 ID: 99094 | Hgene | Tgene | Gene symbol | WDR76 | IDH3A | Gene ID | 79968 | 3419 |
Gene name | WD repeat domain 76 | isocitrate dehydrogenase (NAD(+)) 3 catalytic subunit alpha | |
Synonyms | CDW14 | - | |
Cytomap | 15q15.3 | 15q25.1 | |
Type of gene | protein-coding | protein-coding | |
Description | WD repeat-containing protein 76 | isocitrate dehydrogenase [NAD] subunit alpha, mitochondrialH-IDH alphaNAD(+)-specific ICDH subunit alphaNAD(H)-specific isocitrate dehydrogenase alpha subunitNAD+-specific ICDHisocitrate dehydrogenase (NAD(+)) 3 alphaisocitrate dehydrogenase (NAD+) | |
Modification date | 20200313 | 20200320 | |
UniProtAcc | . | P50213 Main function of 5'-partner protein: FUNCTION: Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers. {ECO:0000269|PubMed:28139779}. | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000263795, ENST00000381246, ENST00000478130, | ENST00000558535, ENST00000561366, ENST00000299518, ENST00000441490, ENST00000559205, ENST00000558554, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 4 X 4 X 3=48 | 2 X 3 X 2=12 |
# samples | 6 | 3 | |
** MAII score | log2(6/48*10)=0.321928094887362 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | log2(3/12*10)=1.32192809488736 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
Fusion gene context | PubMed: WDR76 [Title/Abstract] AND IDH3A [Title/Abstract] AND fusion [Title/Abstract] | ||
Fusion neoantigen context | PubMed: WDR76 [Title/Abstract] AND IDH3A [Title/Abstract] AND neoantigen [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | WDR76(44120564)-IDH3A(78461264), # samples:2 | ||
Anticipated loss of major functional domain due to fusion event. | WDR76-IDH3A seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. WDR76-IDH3A seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr15:44120564/chr15:78461264) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Amino Acid Sequences |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000263795 | WDR76 | chr15 | 44120564 | + | ENST00000558554 | IDH3A | chr15 | 78461264 | + | 1171 | 532 | 34 | 615 | 193 |
ENST00000381246 | WDR76 | chr15 | 44120564 | + | ENST00000558554 | IDH3A | chr15 | 78461264 | + | 1180 | 541 | 181 | 624 | 147 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000263795 | ENST00000558554 | WDR76 | chr15 | 44120564 | + | IDH3A | chr15 | 78461264 | + | 0.003682466 | 0.9963175 |
ENST00000381246 | ENST00000558554 | WDR76 | chr15 | 44120564 | + | IDH3A | chr15 | 78461264 | + | 0.027578812 | 0.97242117 |
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Get the fusion protein sequences from here. |
Fusion protein sequence information is available in the fasta format. >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP |
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Fusion Protein Breakpoint Sequences for WDR76-IDH3A |
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Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Length(fusion protein) | BP in fusion protein | Peptide |
WDR76 | chr15 | 44120564 | IDH3A | chr15 | 78461264 | 532 | 164 | SSQDGDSDEDTTPSLSLTKDLGGNAK |
WDR76 | chr15 | 44120564 | IDH3A | chr15 | 78461264 | 541 | 118 | SSQDGDSDEDTTPSLSLTKDLGGNAK |
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Potential FusionNeoAntigen Information of WDR76-IDH3A in HLA I |
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WDR76-IDH3A_44120564_78461264.msa |
![]() * We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5) |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA I | FusionNeoAntigen peptide | Binding score | Immunogenic score | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:01 | DEDTTPSL | 0.9919 | 0.9656 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:13 | SDEDTTPSL | 0.2772 | 0.8921 | 6 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:08 | DEDTTPSL | 0.9684 | 0.8127 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:08 | SDEDTTPSL | 0.6527 | 0.7349 | 6 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:08 | DEDTTPSLSL | 0.9047 | 0.7672 | 7 | 17 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B42:01 | TPSLSLTKDL | 0.6065 | 0.5246 | 11 | 21 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:04 | DEDTTPSL | 0.993 | 0.9702 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:07 | DEDTTPSL | 0.992 | 0.9402 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:05 | DEDTTPSL | 0.9919 | 0.9656 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:06 | DEDTTPSL | 0.9893 | 0.9685 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:03 | DEDTTPSL | 0.9889 | 0.963 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:08 | DEDTTPSL | 0.9856 | 0.9535 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:31 | DEDTTPSL | 0.9671 | 0.959 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:11 | DEDTTPSL | 0.9592 | 0.7697 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:11 | DEDTTPSL | 0.8742 | 0.9589 | 7 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:11 | SDEDTTPSL | 0.6486 | 0.6884 | 6 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:02 | SDEDTTPSL | 0.3588 | 0.9029 | 6 | 15 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B39:11 | DEDTTPSLSL | 0.9029 | 0.687 | 7 | 17 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B18:03 | DEDTTPSLSL | 0.562 | 0.8973 | 7 | 17 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | HLA-B67:01 | TPSLSLTKDL | 0.3482 | 0.818 | 11 | 21 |
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Potential FusionNeoAntigen Information of WDR76-IDH3A in HLA II |
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WDR76-IDH3A_44120564_78461264.msa |
![]() * We used NetMHCIIpan v4.1 (%rank<0.5). |
Fusion gene | Hchr | Hbp | Tgene | Tchr | Tbp | HLA II | FusionNeoAntigen peptide | Neoantigen start (at BP 13) | Neoantigen end (at BP 13) |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | DRB1-0450 | TPSLSLTKDLGGNAK | 11 | 26 |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 | DRB1-0453 | TPSLSLTKDLGGNAK | 11 | 26 |
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Fusion breakpoint peptide structures of WDR76-IDH3A |
![]() * The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA. |
File name | BPseq | Hgene | Tgene | Hchr | Hbp | Tchr | Tbp | AAlen |
8460 | SDEDTTPSLSLTKD | WDR76 | IDH3A | chr15 | 44120564 | chr15 | 78461264 | 532 |
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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of WDR76-IDH3A |
![]() * We used Glide to predict the interaction between HLAs and neoantigens. |
HLA allele | PDB ID | File name | BPseq | Docking score | Glide score |
HLA-B14:02 | 3BVN | 8460 | SDEDTTPSLSLTKD | -7.15543 | -7.26883 |
HLA-B14:02 | 3BVN | 8460 | SDEDTTPSLSLTKD | -4.77435 | -5.80965 |
HLA-B52:01 | 3W39 | 8460 | SDEDTTPSLSLTKD | -6.80875 | -6.92215 |
HLA-B52:01 | 3W39 | 8460 | SDEDTTPSLSLTKD | -4.20386 | -5.23916 |
HLA-A11:01 | 4UQ2 | 8460 | SDEDTTPSLSLTKD | -7.5194 | -8.5547 |
HLA-A11:01 | 4UQ2 | 8460 | SDEDTTPSLSLTKD | -6.9601 | -7.0735 |
HLA-A24:02 | 5HGA | 8460 | SDEDTTPSLSLTKD | -7.52403 | -7.63743 |
HLA-A24:02 | 5HGA | 8460 | SDEDTTPSLSLTKD | -5.82433 | -6.85963 |
HLA-B27:05 | 6PYJ | 8460 | SDEDTTPSLSLTKD | -3.28285 | -4.31815 |
HLA-B44:05 | 3DX8 | 8460 | SDEDTTPSLSLTKD | -5.91172 | -6.94702 |
HLA-B44:05 | 3DX8 | 8460 | SDEDTTPSLSLTKD | -4.24346 | -4.35686 |
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Vaccine Design for the FusionNeoAntigens of WDR76-IDH3A |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide sequence | FusionNeoAntigen RNA sequence |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 11 | 21 | TPSLSLTKDL | TCCCTGAGCTTGACAAAAGATTTGGGAGGC |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 6 | 15 | SDEDTTPSL | GAAGATACCACACCATCCCTGAGCTTG |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 7 | 15 | DEDTTPSL | GATACCACACCATCCCTGAGCTTG |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 7 | 17 | DEDTTPSLSL | GATACCACACCATCCCTGAGCTTGACAAAA |
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Fusion gene | Hchr | Hbp | Tchr | Tbp | Start in +/-13AA | End in +/-13AA | FusionNeoAntigen peptide | FusionNEoAntigen RNA sequence |
WDR76-IDH3A | chr15 | 44120564 | chr15 | 78461264 | 11 | 26 | TPSLSLTKDLGGNAK | TCCCTGAGCTTGACAAAAGATTTGGGAGGCAATGCAAAATGCTCA |
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Information of the samples that have these potential fusion neoantigens of WDR76-IDH3A |
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Cancer type | Fusion gene | Hchr | Hbp | Henst | Tchr | Tbp | Tenst | Sample |
SKCM | WDR76-IDH3A | chr15 | 44120564 | ENST00000263795 | chr15 | 78461264 | ENST00000558554 | TCGA-WE-A8K1-06A |
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Potential target of CAR-T therapy development for WDR76-IDH3A |
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![]() * Minus value of BPloci means that the break point is located before the CDS. |
- In-frame and retained 'Transmembrane'. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
![]() * We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image. |
Hgene | Hchr | Hbp | Henst | Tgene | Tchr | Tbp | Tenst | DeepLoc result |
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Related Drugs to WDR76-IDH3A |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to WDR76-IDH3A |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |