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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:WNK1-ERC1

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: WNK1-ERC1
FusionPDB ID: 99327
FusionGDB2.0 ID: 99327
HgeneTgene
Gene symbol

WNK1

ERC1

Gene ID

65125

23085

Gene nameWNK lysine deficient protein kinase 1ELKS/RAB6-interacting/CAST family member 1
SynonymsHSAN2|HSN2|KDP|PPP1R167|PRKWNK1|PSK|p65Cast2|ELKS|ERC-1|RAB6IP2
Cytomap

12p13.33

12p13.33

Type of geneprotein-codingprotein-coding
Descriptionserine/threonine-protein kinase WNK1WNK lysine deficient protein kinase 1 isoformerythrocyte 65 kDa proteinprostate-derived sterile 20-like kinaseprotein kinase with no lysine 1protein phosphatase 1, regulatory subunit 167serine/threonine-protein kiELKS/Rab6-interacting/CAST family member 1RAB6 interacting protein 2
Modification date2020032720200313
UniProtAcc.

Q8IUD2

Main function of 5'-partner protein: FUNCTION: Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. {ECO:0000269|PubMed:15218148}.
Ensembl transtripts involved in fusion geneENST idsENST00000315939, ENST00000447667, 
ENST00000530271, ENST00000535572, 
ENST00000537687, ENST00000340908, 
ENST00000540360, ENST00000574564, 
ENST00000536573, ENST00000360905, 
ENST00000397203, ENST00000543086, 
ENST00000546231, ENST00000589028, 
ENST00000355446, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score23 X 19 X 15=655523 X 29 X 12=8004
# samples 3733
** MAII scorelog2(37/6555*10)=-4.14699860459281
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(33/8004*10)=-4.60018323765993
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: WNK1 [Title/Abstract] AND ERC1 [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: WNK1 [Title/Abstract] AND ERC1 [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ERC1(1213990)-WNK1(987377), # samples:2
ERC1(1213990)-WNK1(977032), # samples:2
ERC1(1137738)-WNK1(987378), # samples:2
WNK1(922980)-ERC1(1289705), # samples:2
Anticipated loss of major functional domain due to fusion event.ERC1-WNK1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ERC1-WNK1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
WNK1-ERC1 seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF.
WNK1-ERC1 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
WNK1-ERC1 seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneWNK1

GO:0006468

protein phosphorylation

10660600

HgeneWNK1

GO:0010923

negative regulation of phosphatase activity

19389623

HgeneWNK1

GO:0023016

signal transduction by trans-phosphorylation

16669787

HgeneWNK1

GO:0035556

intracellular signal transduction

10660600

TgeneERC1

GO:0007252

I-kappaB phosphorylation

15218148



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:1213990/chr12:987377)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across WNK1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across ERC1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000535572WNK1chr12863490+ENST00000543086ERC1chr121192329+10033173282643291167
ENST00000535572WNK1chr12863490+ENST00000546231ERC1chr121192329+4925173282644251199
ENST00000535572WNK1chr12863490+ENST00000397203ERC1chr121192329+10108173282644131195
ENST00000535572WNK1chr12863490+ENST00000360905ERC1chr121192329+6671173282644131195
ENST00000535572WNK1chr12863490+ENST00000589028ERC1chr121192329+10108173282644131195
ENST00000535572WNK1chr12863490+ENST00000355446ERC1chr121192329+6727173282643231165
ENST00000537687WNK1chr12863490+ENST00000543086ERC1chr121192329+9703140249639991167
ENST00000537687WNK1chr12863490+ENST00000546231ERC1chr121192329+4595140249640951199
ENST00000537687WNK1chr12863490+ENST00000397203ERC1chr121192329+9778140249640831195
ENST00000537687WNK1chr12863490+ENST00000360905ERC1chr121192329+6341140249640831195
ENST00000537687WNK1chr12863490+ENST00000589028ERC1chr121192329+9778140249640831195
ENST00000537687WNK1chr12863490+ENST00000355446ERC1chr121192329+6397140249639931165
ENST00000315939WNK1chr12863490+ENST00000543086ERC1chr121192329+9703140249639991167
ENST00000315939WNK1chr12863490+ENST00000546231ERC1chr121192329+4595140249640951199
ENST00000315939WNK1chr12863490+ENST00000397203ERC1chr121192329+9778140249640831195
ENST00000315939WNK1chr12863490+ENST00000360905ERC1chr121192329+6341140249640831195
ENST00000315939WNK1chr12863490+ENST00000589028ERC1chr121192329+9778140249640831195
ENST00000315939WNK1chr12863490+ENST00000355446ERC1chr121192329+6397140249639931165
ENST00000447667WNK1chr12863490+ENST00000543086ERC1chr121192329+9570126936338661167
ENST00000447667WNK1chr12863490+ENST00000546231ERC1chr121192329+4462126936339621199
ENST00000447667WNK1chr12863490+ENST00000397203ERC1chr121192329+9645126936339501195
ENST00000447667WNK1chr12863490+ENST00000360905ERC1chr121192329+6208126936339501195
ENST00000447667WNK1chr12863490+ENST00000589028ERC1chr121192329+9645126936339501195
ENST00000447667WNK1chr12863490+ENST00000355446ERC1chr121192329+6264126936338601165
ENST00000530271WNK1chr12863490+ENST00000543086ERC1chr121192329+9060759033561118
ENST00000530271WNK1chr12863490+ENST00000546231ERC1chr121192329+3952759034521150
ENST00000530271WNK1chr12863490+ENST00000397203ERC1chr121192329+9135759034401146
ENST00000530271WNK1chr12863490+ENST00000360905ERC1chr121192329+5698759034401146
ENST00000530271WNK1chr12863490+ENST00000589028ERC1chr121192329+9135759034401146
ENST00000530271WNK1chr12863490+ENST00000355446ERC1chr121192329+5754759033501116
ENST00000535572WNK1chr12863490+ENST00000543086ERC1chr121289706+904917328263345839
ENST00000535572WNK1chr12863490+ENST00000546231ERC1chr121289706+385717328263357843
ENST00000535572WNK1chr12863490+ENST00000397203ERC1chr121289706+904017328263345839
ENST00000535572WNK1chr12863490+ENST00000360905ERC1chr121289706+560317328263345839
ENST00000535572WNK1chr12863490+ENST00000589028ERC1chr121289706+904017328263345839
ENST00000535572WNK1chr12863490+ENST00000355446ERC1chr121289706+565917328263255809
ENST00000537687WNK1chr12863490+ENST00000543086ERC1chr121289706+871914024963015839
ENST00000537687WNK1chr12863490+ENST00000546231ERC1chr121289706+352714024963027843
ENST00000537687WNK1chr12863490+ENST00000397203ERC1chr121289706+871014024963015839
ENST00000537687WNK1chr12863490+ENST00000360905ERC1chr121289706+527314024963015839
ENST00000537687WNK1chr12863490+ENST00000589028ERC1chr121289706+871014024963015839
ENST00000537687WNK1chr12863490+ENST00000355446ERC1chr121289706+532914024962925809
ENST00000315939WNK1chr12863490+ENST00000543086ERC1chr121289706+871914024963015839
ENST00000315939WNK1chr12863490+ENST00000546231ERC1chr121289706+352714024963027843
ENST00000315939WNK1chr12863490+ENST00000397203ERC1chr121289706+871014024963015839
ENST00000315939WNK1chr12863490+ENST00000360905ERC1chr121289706+527314024963015839
ENST00000315939WNK1chr12863490+ENST00000589028ERC1chr121289706+871014024963015839
ENST00000315939WNK1chr12863490+ENST00000355446ERC1chr121289706+532914024962925809
ENST00000447667WNK1chr12863490+ENST00000543086ERC1chr121289706+858612693632882839
ENST00000447667WNK1chr12863490+ENST00000546231ERC1chr121289706+339412693632894843
ENST00000447667WNK1chr12863490+ENST00000397203ERC1chr121289706+857712693632882839
ENST00000447667WNK1chr12863490+ENST00000360905ERC1chr121289706+514012693632882839
ENST00000447667WNK1chr12863490+ENST00000589028ERC1chr121289706+857712693632882839
ENST00000447667WNK1chr12863490+ENST00000355446ERC1chr121289706+519612693632792809
ENST00000530271WNK1chr12863490+ENST00000543086ERC1chr121289706+807675902372790
ENST00000530271WNK1chr12863490+ENST00000546231ERC1chr121289706+288475902384794
ENST00000530271WNK1chr12863490+ENST00000397203ERC1chr121289706+806775902372790
ENST00000530271WNK1chr12863490+ENST00000360905ERC1chr121289706+463075902372790
ENST00000530271WNK1chr12863490+ENST00000589028ERC1chr121289706+806775902372790
ENST00000530271WNK1chr12863490+ENST00000355446ERC1chr121289706+468675902282760
ENST00000535572WNK1chr12863490+ENST00000543086ERC1chr121299166+848717321658132508
ENST00000535572WNK1chr12863490+ENST00000546231ERC1chr121299166+329517321658132508
ENST00000535572WNK1chr12863490+ENST00000397203ERC1chr121299166+847817321658132508
ENST00000535572WNK1chr12863490+ENST00000360905ERC1chr121299166+504117321658132508
ENST00000535572WNK1chr12863490+ENST00000589028ERC1chr121299166+847817321658132508
ENST00000535572WNK1chr12863490+ENST00000355446ERC1chr121299166+509717321658132508
ENST00000537687WNK1chr12863490+ENST00000543086ERC1chr121299166+8157140213280443
ENST00000537687WNK1chr12863490+ENST00000546231ERC1chr121299166+2965140213280443
ENST00000537687WNK1chr12863490+ENST00000397203ERC1chr121299166+8148140213280443
ENST00000537687WNK1chr12863490+ENST00000360905ERC1chr121299166+4711140213280443
ENST00000537687WNK1chr12863490+ENST00000589028ERC1chr121299166+8148140213280443
ENST00000537687WNK1chr12863490+ENST00000355446ERC1chr121299166+4767140213280443
ENST00000315939WNK1chr12863490+ENST00000543086ERC1chr121299166+8157140213280443
ENST00000315939WNK1chr12863490+ENST00000546231ERC1chr121299166+2965140213280443
ENST00000315939WNK1chr12863490+ENST00000397203ERC1chr121299166+8148140213280443
ENST00000315939WNK1chr12863490+ENST00000360905ERC1chr121299166+4711140213280443
ENST00000315939WNK1chr12863490+ENST00000589028ERC1chr121299166+8148140213280443
ENST00000315939WNK1chr12863490+ENST00000355446ERC1chr121299166+4767140213280443
ENST00000447667WNK1chr12863490+ENST00000543086ERC1chr121299166+8024126911952398
ENST00000447667WNK1chr12863490+ENST00000546231ERC1chr121299166+2832126911952398
ENST00000447667WNK1chr12863490+ENST00000397203ERC1chr121299166+8015126911952398
ENST00000447667WNK1chr12863490+ENST00000360905ERC1chr121299166+4578126911952398
ENST00000447667WNK1chr12863490+ENST00000589028ERC1chr121299166+8015126911952398
ENST00000447667WNK1chr12863490+ENST00000355446ERC1chr121299166+4634126911952398
ENST00000530271WNK1chr12863490+ENST00000543086ERC1chr121299166+75147598031810335
ENST00000530271WNK1chr12863490+ENST00000546231ERC1chr121299166+23227598031822339
ENST00000530271WNK1chr12863490+ENST00000397203ERC1chr121299166+75057598031810335
ENST00000530271WNK1chr12863490+ENST00000360905ERC1chr121299166+40687598031810335
ENST00000530271WNK1chr12863490+ENST00000589028ERC1chr121299166+75057598031810335
ENST00000530271WNK1chr12863490+ENST00000355446ERC1chr121299166+41247598031720305
ENST00000535572WNK1chr12863490+ENST00000543086ERC1chr121192330+10033173282643291167
ENST00000535572WNK1chr12863490+ENST00000546231ERC1chr121192330+4925173282644251199
ENST00000535572WNK1chr12863490+ENST00000397203ERC1chr121192330+10108173282644131195
ENST00000535572WNK1chr12863490+ENST00000360905ERC1chr121192330+6671173282644131195
ENST00000535572WNK1chr12863490+ENST00000589028ERC1chr121192330+10108173282644131195
ENST00000535572WNK1chr12863490+ENST00000355446ERC1chr121192330+6727173282643231165
ENST00000537687WNK1chr12863490+ENST00000543086ERC1chr121192330+9703140249639991167
ENST00000537687WNK1chr12863490+ENST00000546231ERC1chr121192330+4595140249640951199
ENST00000537687WNK1chr12863490+ENST00000397203ERC1chr121192330+9778140249640831195
ENST00000537687WNK1chr12863490+ENST00000360905ERC1chr121192330+6341140249640831195
ENST00000537687WNK1chr12863490+ENST00000589028ERC1chr121192330+9778140249640831195
ENST00000537687WNK1chr12863490+ENST00000355446ERC1chr121192330+6397140249639931165
ENST00000315939WNK1chr12863490+ENST00000543086ERC1chr121192330+9703140249639991167
ENST00000315939WNK1chr12863490+ENST00000546231ERC1chr121192330+4595140249640951199
ENST00000315939WNK1chr12863490+ENST00000397203ERC1chr121192330+9778140249640831195
ENST00000315939WNK1chr12863490+ENST00000360905ERC1chr121192330+6341140249640831195
ENST00000315939WNK1chr12863490+ENST00000589028ERC1chr121192330+9778140249640831195
ENST00000315939WNK1chr12863490+ENST00000355446ERC1chr121192330+6397140249639931165
ENST00000447667WNK1chr12863490+ENST00000543086ERC1chr121192330+9570126936338661167
ENST00000447667WNK1chr12863490+ENST00000546231ERC1chr121192330+4462126936339621199
ENST00000447667WNK1chr12863490+ENST00000397203ERC1chr121192330+9645126936339501195
ENST00000447667WNK1chr12863490+ENST00000360905ERC1chr121192330+6208126936339501195
ENST00000447667WNK1chr12863490+ENST00000589028ERC1chr121192330+9645126936339501195
ENST00000447667WNK1chr12863490+ENST00000355446ERC1chr121192330+6264126936338601165
ENST00000530271WNK1chr12863490+ENST00000543086ERC1chr121192330+9060759033561118
ENST00000530271WNK1chr12863490+ENST00000546231ERC1chr121192330+3952759034521150
ENST00000530271WNK1chr12863490+ENST00000397203ERC1chr121192330+9135759034401146
ENST00000530271WNK1chr12863490+ENST00000360905ERC1chr121192330+5698759034401146
ENST00000530271WNK1chr12863490+ENST00000589028ERC1chr121192330+9135759034401146
ENST00000530271WNK1chr12863490+ENST00000355446ERC1chr121192330+5754759033501116
ENST00000535572WNK1chr12863490+ENST00000543086ERC1chr121517315+786117321658132508
ENST00000535572WNK1chr12863490+ENST00000546231ERC1chr121517315+265717328262157443
ENST00000535572WNK1chr12863490+ENST00000397203ERC1chr121517315+785217321658132508
ENST00000535572WNK1chr12863490+ENST00000360905ERC1chr121517315+441517328262157443
ENST00000535572WNK1chr12863490+ENST00000589028ERC1chr121517315+785217321658132508
ENST00000535572WNK1chr12863490+ENST00000355446ERC1chr121517315+447117321658132508
ENST00000537687WNK1chr12863490+ENST00000543086ERC1chr121517315+753114024961827443
ENST00000537687WNK1chr12863490+ENST00000546231ERC1chr121517315+232714024961827443
ENST00000537687WNK1chr12863490+ENST00000397203ERC1chr121517315+752214024961827443
ENST00000537687WNK1chr12863490+ENST00000360905ERC1chr121517315+408514024961827443
ENST00000537687WNK1chr12863490+ENST00000589028ERC1chr121517315+752214024961827443
ENST00000537687WNK1chr12863490+ENST00000355446ERC1chr121517315+4141140213280443
ENST00000315939WNK1chr12863490+ENST00000543086ERC1chr121517315+753114024961827443
ENST00000315939WNK1chr12863490+ENST00000546231ERC1chr121517315+232714024961827443
ENST00000315939WNK1chr12863490+ENST00000397203ERC1chr121517315+752214024961827443
ENST00000315939WNK1chr12863490+ENST00000360905ERC1chr121517315+408514024961827443
ENST00000315939WNK1chr12863490+ENST00000589028ERC1chr121517315+752214024961827443
ENST00000315939WNK1chr12863490+ENST00000355446ERC1chr121517315+4141140213280443
ENST00000447667WNK1chr12863490+ENST00000543086ERC1chr121517315+739812693631694443
ENST00000447667WNK1chr12863490+ENST00000546231ERC1chr121517315+219412693631694443
ENST00000447667WNK1chr12863490+ENST00000397203ERC1chr121517315+738912693631694443
ENST00000447667WNK1chr12863490+ENST00000360905ERC1chr121517315+395212693631694443
ENST00000447667WNK1chr12863490+ENST00000589028ERC1chr121517315+738912693631694443
ENST00000447667WNK1chr12863490+ENST00000355446ERC1chr121517315+400812693631604413
ENST00000530271WNK1chr12863490+ENST00000543086ERC1chr121517315+688875901184394
ENST00000530271WNK1chr12863490+ENST00000546231ERC1chr121517315+168475901184394
ENST00000530271WNK1chr12863490+ENST00000397203ERC1chr121517315+687975901184394
ENST00000530271WNK1chr12863490+ENST00000360905ERC1chr121517315+344275901184394
ENST00000530271WNK1chr12863490+ENST00000589028ERC1chr121517315+687975901184394
ENST00000530271WNK1chr12863490+ENST00000355446ERC1chr121517315+349875901094364

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000535572ENST00000543086WNK1chr12863490+ERC1chr121192329+0.0064070290.9935929
ENST00000535572ENST00000546231WNK1chr12863490+ERC1chr121192329+0.017246770.9827533
ENST00000535572ENST00000397203WNK1chr12863490+ERC1chr121192329+0.0049733660.99502665
ENST00000535572ENST00000360905WNK1chr12863490+ERC1chr121192329+0.0103086750.9896913
ENST00000535572ENST00000589028WNK1chr12863490+ERC1chr121192329+0.0049733660.99502665
ENST00000535572ENST00000355446WNK1chr12863490+ERC1chr121192329+0.0058277120.9941723
ENST00000537687ENST00000543086WNK1chr12863490+ERC1chr121192329+0.0048759750.99512404
ENST00000537687ENST00000546231WNK1chr12863490+ERC1chr121192329+0.0159282610.9840717
ENST00000537687ENST00000397203WNK1chr12863490+ERC1chr121192329+0.0037288660.99627113
ENST00000537687ENST00000360905WNK1chr12863490+ERC1chr121192329+0.0083223240.99167764
ENST00000537687ENST00000589028WNK1chr12863490+ERC1chr121192329+0.0037288660.99627113
ENST00000537687ENST00000355446WNK1chr12863490+ERC1chr121192329+0.0044340180.99556595
ENST00000315939ENST00000543086WNK1chr12863490+ERC1chr121192329+0.0048759750.99512404
ENST00000315939ENST00000546231WNK1chr12863490+ERC1chr121192329+0.0159282610.9840717
ENST00000315939ENST00000397203WNK1chr12863490+ERC1chr121192329+0.0037288660.99627113
ENST00000315939ENST00000360905WNK1chr12863490+ERC1chr121192329+0.0083223240.99167764
ENST00000315939ENST00000589028WNK1chr12863490+ERC1chr121192329+0.0037288660.99627113
ENST00000315939ENST00000355446WNK1chr12863490+ERC1chr121192329+0.0044340180.99556595
ENST00000447667ENST00000543086WNK1chr12863490+ERC1chr121192329+0.0045568360.9954432
ENST00000447667ENST00000546231WNK1chr12863490+ERC1chr121192329+0.0153477930.9846523
ENST00000447667ENST00000397203WNK1chr12863490+ERC1chr121192329+0.0035093730.99649066
ENST00000447667ENST00000360905WNK1chr12863490+ERC1chr121192329+0.0077390750.992261
ENST00000447667ENST00000589028WNK1chr12863490+ERC1chr121192329+0.0035093730.99649066
ENST00000447667ENST00000355446WNK1chr12863490+ERC1chr121192329+0.0042318970.9957681
ENST00000530271ENST00000543086WNK1chr12863490+ERC1chr121192329+0.0026041720.9973959
ENST00000530271ENST00000546231WNK1chr12863490+ERC1chr121192329+0.0119304810.9880695
ENST00000530271ENST00000397203WNK1chr12863490+ERC1chr121192329+0.0019852670.9980147
ENST00000530271ENST00000360905WNK1chr12863490+ERC1chr121192329+0.0047340010.99526596
ENST00000530271ENST00000589028WNK1chr12863490+ERC1chr121192329+0.0019852670.9980147
ENST00000530271ENST00000355446WNK1chr12863490+ERC1chr121192329+0.002441020.99755895
ENST00000535572ENST00000543086WNK1chr12863490+ERC1chr121289706+0.0016500910.9983499
ENST00000535572ENST00000546231WNK1chr12863490+ERC1chr121289706+0.0094662890.9905337
ENST00000535572ENST00000397203WNK1chr12863490+ERC1chr121289706+0.0016830460.998317
ENST00000535572ENST00000360905WNK1chr12863490+ERC1chr121289706+0.0043063950.99569356
ENST00000535572ENST00000589028WNK1chr12863490+ERC1chr121289706+0.0016830460.998317
ENST00000535572ENST00000355446WNK1chr12863490+ERC1chr121289706+0.0047199170.99528015
ENST00000537687ENST00000543086WNK1chr12863490+ERC1chr121289706+0.0015276680.9984723
ENST00000537687ENST00000546231WNK1chr12863490+ERC1chr121289706+0.0096354040.9903646
ENST00000537687ENST00000397203WNK1chr12863490+ERC1chr121289706+0.0015594810.99844056
ENST00000537687ENST00000360905WNK1chr12863490+ERC1chr121289706+0.00410460.9958954
ENST00000537687ENST00000589028WNK1chr12863490+ERC1chr121289706+0.0015594810.99844056
ENST00000537687ENST00000355446WNK1chr12863490+ERC1chr121289706+0.0045590990.99544084
ENST00000315939ENST00000543086WNK1chr12863490+ERC1chr121289706+0.0015276680.9984723
ENST00000315939ENST00000546231WNK1chr12863490+ERC1chr121289706+0.0096354040.9903646
ENST00000315939ENST00000397203WNK1chr12863490+ERC1chr121289706+0.0015594810.99844056
ENST00000315939ENST00000360905WNK1chr12863490+ERC1chr121289706+0.00410460.9958954
ENST00000315939ENST00000589028WNK1chr12863490+ERC1chr121289706+0.0015594810.99844056
ENST00000315939ENST00000355446WNK1chr12863490+ERC1chr121289706+0.0045590990.99544084
ENST00000447667ENST00000543086WNK1chr12863490+ERC1chr121289706+0.0015651580.99843484
ENST00000447667ENST00000546231WNK1chr12863490+ERC1chr121289706+0.0103050510.98969495
ENST00000447667ENST00000397203WNK1chr12863490+ERC1chr121289706+0.0015994940.9984005
ENST00000447667ENST00000360905WNK1chr12863490+ERC1chr121289706+0.0043147040.99568534
ENST00000447667ENST00000589028WNK1chr12863490+ERC1chr121289706+0.0015994940.9984005
ENST00000447667ENST00000355446WNK1chr12863490+ERC1chr121289706+0.0046852180.99531484
ENST00000530271ENST00000543086WNK1chr12863490+ERC1chr121289706+0.0012934730.9987066
ENST00000530271ENST00000546231WNK1chr12863490+ERC1chr121289706+0.0082504040.99174964
ENST00000530271ENST00000397203WNK1chr12863490+ERC1chr121289706+0.0013234560.9986765
ENST00000530271ENST00000360905WNK1chr12863490+ERC1chr121289706+0.0030763360.9969236
ENST00000530271ENST00000589028WNK1chr12863490+ERC1chr121289706+0.0013234560.9986765
ENST00000530271ENST00000355446WNK1chr12863490+ERC1chr121289706+0.0034194210.99658054
ENST00000535572ENST00000543086WNK1chr12863490+ERC1chr121299166+0.0008714090.99912864
ENST00000535572ENST00000546231WNK1chr12863490+ERC1chr121299166+0.0026585790.99734145
ENST00000535572ENST00000397203WNK1chr12863490+ERC1chr121299166+0.0008949670.99910504
ENST00000535572ENST00000360905WNK1chr12863490+ERC1chr121299166+0.0015821830.99841785
ENST00000535572ENST00000589028WNK1chr12863490+ERC1chr121299166+0.0008949670.99910504
ENST00000535572ENST00000355446WNK1chr12863490+ERC1chr121299166+0.0019119910.99808794
ENST00000537687ENST00000543086WNK1chr12863490+ERC1chr121299166+0.0008283350.99917173
ENST00000537687ENST00000546231WNK1chr12863490+ERC1chr121299166+0.0026470430.99735296
ENST00000537687ENST00000397203WNK1chr12863490+ERC1chr121299166+0.0008503440.9991497
ENST00000537687ENST00000360905WNK1chr12863490+ERC1chr121299166+0.0015940810.998406
ENST00000537687ENST00000589028WNK1chr12863490+ERC1chr121299166+0.0008503440.9991497
ENST00000537687ENST00000355446WNK1chr12863490+ERC1chr121299166+0.00189980.9981002
ENST00000315939ENST00000543086WNK1chr12863490+ERC1chr121299166+0.0008283350.99917173
ENST00000315939ENST00000546231WNK1chr12863490+ERC1chr121299166+0.0026470430.99735296
ENST00000315939ENST00000397203WNK1chr12863490+ERC1chr121299166+0.0008503440.9991497
ENST00000315939ENST00000360905WNK1chr12863490+ERC1chr121299166+0.0015940810.998406
ENST00000315939ENST00000589028WNK1chr12863490+ERC1chr121299166+0.0008503440.9991497
ENST00000315939ENST00000355446WNK1chr12863490+ERC1chr121299166+0.00189980.9981002
ENST00000447667ENST00000543086WNK1chr12863490+ERC1chr121299166+0.000806410.9991936
ENST00000447667ENST00000546231WNK1chr12863490+ERC1chr121299166+0.002527830.9974721
ENST00000447667ENST00000397203WNK1chr12863490+ERC1chr121299166+0.0008287130.99917126
ENST00000447667ENST00000360905WNK1chr12863490+ERC1chr121299166+0.0015022780.9984977
ENST00000447667ENST00000589028WNK1chr12863490+ERC1chr121299166+0.0008287130.99917126
ENST00000447667ENST00000355446WNK1chr12863490+ERC1chr121299166+0.0017478230.9982522
ENST00000530271ENST00000543086WNK1chr12863490+ERC1chr121299166+0.0007289530.9992711
ENST00000530271ENST00000546231WNK1chr12863490+ERC1chr121299166+0.0020158970.99798405
ENST00000530271ENST00000397203WNK1chr12863490+ERC1chr121299166+0.0007504170.9992495
ENST00000530271ENST00000360905WNK1chr12863490+ERC1chr121299166+0.0011072060.99889284
ENST00000530271ENST00000589028WNK1chr12863490+ERC1chr121299166+0.0007504170.9992495
ENST00000530271ENST00000355446WNK1chr12863490+ERC1chr121299166+0.0013662420.9986337
ENST00000535572ENST00000543086WNK1chr12863490+ERC1chr121192330+0.0064070290.9935929
ENST00000535572ENST00000546231WNK1chr12863490+ERC1chr121192330+0.017246770.9827533
ENST00000535572ENST00000397203WNK1chr12863490+ERC1chr121192330+0.0049733660.99502665
ENST00000535572ENST00000360905WNK1chr12863490+ERC1chr121192330+0.0103086750.9896913
ENST00000535572ENST00000589028WNK1chr12863490+ERC1chr121192330+0.0049733660.99502665
ENST00000535572ENST00000355446WNK1chr12863490+ERC1chr121192330+0.0058277120.9941723
ENST00000537687ENST00000543086WNK1chr12863490+ERC1chr121192330+0.0048759750.99512404
ENST00000537687ENST00000546231WNK1chr12863490+ERC1chr121192330+0.0159282610.9840717
ENST00000537687ENST00000397203WNK1chr12863490+ERC1chr121192330+0.0037288660.99627113
ENST00000537687ENST00000360905WNK1chr12863490+ERC1chr121192330+0.0083223240.99167764
ENST00000537687ENST00000589028WNK1chr12863490+ERC1chr121192330+0.0037288660.99627113
ENST00000537687ENST00000355446WNK1chr12863490+ERC1chr121192330+0.0044340180.99556595
ENST00000315939ENST00000543086WNK1chr12863490+ERC1chr121192330+0.0048759750.99512404
ENST00000315939ENST00000546231WNK1chr12863490+ERC1chr121192330+0.0159282610.9840717
ENST00000315939ENST00000397203WNK1chr12863490+ERC1chr121192330+0.0037288660.99627113
ENST00000315939ENST00000360905WNK1chr12863490+ERC1chr121192330+0.0083223240.99167764
ENST00000315939ENST00000589028WNK1chr12863490+ERC1chr121192330+0.0037288660.99627113
ENST00000315939ENST00000355446WNK1chr12863490+ERC1chr121192330+0.0044340180.99556595
ENST00000447667ENST00000543086WNK1chr12863490+ERC1chr121192330+0.0045568360.9954432
ENST00000447667ENST00000546231WNK1chr12863490+ERC1chr121192330+0.0153477930.9846523
ENST00000447667ENST00000397203WNK1chr12863490+ERC1chr121192330+0.0035093730.99649066
ENST00000447667ENST00000360905WNK1chr12863490+ERC1chr121192330+0.0077390750.992261
ENST00000447667ENST00000589028WNK1chr12863490+ERC1chr121192330+0.0035093730.99649066
ENST00000447667ENST00000355446WNK1chr12863490+ERC1chr121192330+0.0042318970.9957681
ENST00000530271ENST00000543086WNK1chr12863490+ERC1chr121192330+0.0026041720.9973959
ENST00000530271ENST00000546231WNK1chr12863490+ERC1chr121192330+0.0119304810.9880695
ENST00000530271ENST00000397203WNK1chr12863490+ERC1chr121192330+0.0019852670.9980147
ENST00000530271ENST00000360905WNK1chr12863490+ERC1chr121192330+0.0047340010.99526596
ENST00000530271ENST00000589028WNK1chr12863490+ERC1chr121192330+0.0019852670.9980147
ENST00000530271ENST00000355446WNK1chr12863490+ERC1chr121192330+0.002441020.99755895
ENST00000535572ENST00000543086WNK1chr12863490+ERC1chr121517315+0.0018606710.99813926
ENST00000535572ENST00000546231WNK1chr12863490+ERC1chr121517315+0.0087951610.9912048
ENST00000535572ENST00000397203WNK1chr12863490+ERC1chr121517315+0.0018903570.9981097
ENST00000535572ENST00000360905WNK1chr12863490+ERC1chr121517315+0.0042709680.995729
ENST00000535572ENST00000589028WNK1chr12863490+ERC1chr121517315+0.0018903570.9981097
ENST00000535572ENST00000355446WNK1chr12863490+ERC1chr121517315+0.0049607390.9950393
ENST00000537687ENST00000543086WNK1chr12863490+ERC1chr121517315+0.0017919860.99820805
ENST00000537687ENST00000546231WNK1chr12863490+ERC1chr121517315+0.0101858380.9898142
ENST00000537687ENST00000397203WNK1chr12863490+ERC1chr121517315+0.0018203110.9981797
ENST00000537687ENST00000360905WNK1chr12863490+ERC1chr121517315+0.0041770940.9958229
ENST00000537687ENST00000589028WNK1chr12863490+ERC1chr121517315+0.0018203110.9981797
ENST00000537687ENST00000355446WNK1chr12863490+ERC1chr121517315+0.0046384060.9953616
ENST00000315939ENST00000543086WNK1chr12863490+ERC1chr121517315+0.0017919860.99820805
ENST00000315939ENST00000546231WNK1chr12863490+ERC1chr121517315+0.0101858380.9898142
ENST00000315939ENST00000397203WNK1chr12863490+ERC1chr121517315+0.0018203110.9981797
ENST00000315939ENST00000360905WNK1chr12863490+ERC1chr121517315+0.0041770940.9958229
ENST00000315939ENST00000589028WNK1chr12863490+ERC1chr121517315+0.0018203110.9981797
ENST00000315939ENST00000355446WNK1chr12863490+ERC1chr121517315+0.0046384060.9953616
ENST00000447667ENST00000543086WNK1chr12863490+ERC1chr121517315+0.0017226380.9982774
ENST00000447667ENST00000546231WNK1chr12863490+ERC1chr121517315+0.0092547690.9907452
ENST00000447667ENST00000397203WNK1chr12863490+ERC1chr121517315+0.0017504730.9982495
ENST00000447667ENST00000360905WNK1chr12863490+ERC1chr121517315+0.0038899180.99611014
ENST00000447667ENST00000589028WNK1chr12863490+ERC1chr121517315+0.0017504730.9982495
ENST00000447667ENST00000355446WNK1chr12863490+ERC1chr121517315+0.0042377080.9957623
ENST00000530271ENST00000543086WNK1chr12863490+ERC1chr121517315+0.0012735290.9987264
ENST00000530271ENST00000546231WNK1chr12863490+ERC1chr121517315+0.0074839630.9925161
ENST00000530271ENST00000397203WNK1chr12863490+ERC1chr121517315+0.0012997630.99870026
ENST00000530271ENST00000360905WNK1chr12863490+ERC1chr121517315+0.0026613310.9973387
ENST00000530271ENST00000589028WNK1chr12863490+ERC1chr121517315+0.0012997630.99870026
ENST00000530271ENST00000355446WNK1chr12863490+ERC1chr121517315+0.0028192290.9971808

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for WNK1-ERC1

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide

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Potential FusionNeoAntigen Information of WNK1-ERC1 in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Potential FusionNeoAntigen Information of WNK1-ERC1 in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of WNK1-ERC1

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of WNK1-ERC1

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score

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Vaccine Design for the FusionNeoAntigens of WNK1-ERC1

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of WNK1-ERC1

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample

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Potential target of CAR-T therapy development for WNK1-ERC1

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to WNK1-ERC1

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to WNK1-ERC1

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
TgeneERC1C0238463Papillary thyroid carcinoma1ORPHANET
TgeneERC1C4749581Distal monosomy 12p1ORPHANET