UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine
leaf

Kinase Fusion Gene Summary

leaf

Kinase Fusion Gene Sample Information

leaf

Kinase Fusion ORF Analysis

leaf

Kinase Fusion Amino Acid Sequences

leaf

Multiple Sequence Alignment of All Fusion Protein Isoforms

leaf

Kinase Fusion Protein Functional Features

leaf

Kinase Fusion Protein Structures

leaf

Comparison of Fusion Protein Isoforms

leaf

Comparison of Fusion Protein Sequences/Structures with Known Sequences/Structures from PDB

leaf

pLDDT Scores and Difference Analysis of pLDDT Scores Between the Active Sites (Best) and Non-Active Sites.

leaf

Ramachandran Plot of Kinase Fusion Protein Structure

leaf

Potential Active Site Information

leaf

Virtual Screening Results

leaf

Kinase-Substrate Information

leaf

Related Drugs with This Kinase Fusion Protein

leaf

Related Disease with This Kinase Fusion Protein

leaf

Clinical Trials of the Found Drugs/Small Molecules

Kinase Fusion Gene:ACTG2_PRKCI

Kinase Fusion Protein Summary

check button Kinase Fusion gene summary
Kinase Fusion partner gene informationKinase Fusion gene name: ACTG2_PRKCI
KinaseFusionDB ID: KFG128
FusionGDB2.0 ID: KFG128
HgeneTgene
Gene symbol

ACTG2

PRKCI

Gene ID

72

5584

Gene nameactin gamma 2, smooth muscleprotein kinase C iota
SynonymsACT|ACTA3|ACTE|ACTL3|ACTSG|MMIHS5|VSCM|VSCM1DXS1179E|PKCI|nPKC-iota
Cytomap

2p13.1

3q26.2

Type of geneprotein-codingprotein-coding
Descriptionactin, gamma-enteric smooth muscleactin, gamma 2, smooth muscle, entericactin-like proteinalpha-actin-3protein kinase C iota typePRKC-lambda/iotaaPKC-lambda/iotaatypical protein kinase C-lambda/iota
Modification date2024041120240403
UniProtAcc

P63267

P41743

Ensembl transtripts involved in fusion geneENST idsENST00000345517, ENST00000409624, 
ENST00000409731, ENST00000409918, 
ENST00000295797, 
Context (manual curation of fusion genes in KinaseFusionDB)

PubMed: ACTG2 [Title/Abstract] AND PRKCI [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ACTG2(74146679)-PRKCI(170016880), # samples:1
check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
TgenePRKCI

GO:0006468

protein phosphorylation

8226978

TgenePRKCI

GO:0010976

positive regulation of neuron projection development

36250347

TgenePRKCI

GO:0043524

negative regulation of neuron apoptotic process

10467349

TgenePRKCI

GO:0045197

establishment or maintenance of epithelial cell apical/basal polarity

11257119

TgenePRKCI

GO:0051092

positive regulation of NF-kappaB transcription factor activity

10467349


check buttonKinase Fusion gene breakpoints across ACTG2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonKinase Fusion gene breakpoints across PRKCI (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.


Top

Kinase Fusion Gene Sample Information

check buttonKinase Fusion gene information.
check button Kinase Fusion gene information from four resources (ChiTars 5.0, ChimerDB 4.0, COSMIC, and CCLE)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceSampleHgeneHchrHbpTgeneTchrTbp
ChiTaRS5.0AI970867ACTG2chr2

74146679

PRKCIchr3

170016880



Top

Kinase Fusion ORF Analysis


check buttonKinase Fusion information from ORFfinder translation from full-length transcript sequence from KinaseFusionDB.
HenstTenstHgeneHchrHbpTgeneTchrTbpSeq length
(transcript)		Seq length
(amino acids)

Top

Kinase Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from KinaseFusionDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>Henst_Tenst_Hgene_Hchr_Hbp_Tgene_Tchr_Tbp_length(fusion AA)_AAseq

Multiple Sequence Alignment of All Fusion Protein Isoforms



Top

Kinase Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:74146679/:170016880)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ACTG2

P63267

PRKCI

P41743

FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.FUNCTION: Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation (By similarity). {ECO:0000250|UniProtKB:F1M7Y5, ECO:0000269|PubMed:10356400, ECO:0000269|PubMed:10467349, ECO:0000269|PubMed:10906326, ECO:0000269|PubMed:11042363, ECO:0000269|PubMed:11724794, ECO:0000269|PubMed:12871960, ECO:0000269|PubMed:14684752, ECO:0000269|PubMed:15994303, ECO:0000269|PubMed:18270268, ECO:0000269|PubMed:19327373, ECO:0000269|PubMed:21189248, ECO:0000269|PubMed:21419810, ECO:0000269|PubMed:8226978, ECO:0000269|PubMed:9346882}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.

check button - Retained domain in the 5'-partner of fusion protein (protein functional feature from UniProt).
PartnerHgeneeneHbpTgeneeneTbpENSTBPexonTotalExonProtein feature lociBPlociTotalLenFeatureNote


check button - Retained domain in the 3'-partner of fusion protein (protein functional feature from UniProt).
PartnerHgeneeneHbpTgeneeneTbpENSTBPexonTotalExonProtein feature lociBPlociTotalLenFeatureNote


Top

Kinase-Substrate Information of ACTG2_PRKCI


check button Phosphorylation target of the kinase
(phosphosite, 03-17-2024)
KinaseKinase UniProt AccKinase speciesSubstrateSubstrate UniProt AccSubstrate phosphorylated residuesSubstrate phosphorylated sites (+/-7AA)Domain
PRKCIP41743humanMARK2Q7KZI7T596rGVssRstFHAGQLR
PRKCIP41743humanAMOTQ4VCS5T750DMEGRIKtLHAQIIEAngiomotin_C
PRKCIP41743humanMAPTP10636-8S352DFKDrVQskIGsLDNTubulin-binding
PRKCIP41743humanROCK1Q13464T1334AsPRtLstRstANQs
PRKCIP41743humanEZRP15311T567QGRDkyKtLRQIRQGERM_C
PRKCIP41743humanSP1P08047S59GGQEsQPsPLALLAA
PRKCIP41743humanROCK1Q13464S1333RAsPRtLstRstANQ
PRKCIP41743humanNUMBP49757S295sPFKRQLsLRINELPNumbF
PRKCIP41743humanMARK3P27448T564RGTASRStFHGQPRE
PRKCIP41743humanCDK7P50613T170GsPNRAytHQVVtRWPkinase
PRKCIP41743humanGLI1P08151S243DCRWDGCsQEFDSQE
PRKCIP41743humanROCK1Q13464T1337RtLstRstANQsFRK
PRKCIP41743humanNUMBP49757S7_MNKLRQsFRRKkDV
PRKCIP41743humanNUMBP49757S276EQLARQGsFrGFPALNumbF
PRKCIP41743humanMAPTP10636-8S324kVTskCGsLGNIHHkTubulin-binding
PRKCIP41743humanPPP1R14CQ8TAE6T73RHQQGKVtVkYDRKEPP1_inhibitor
PRKCIP41743humanFBXW7Q969H0S10QELLSVGsKRRRTGG
PRKCIP41743humanDOC2BQ14184S34IRPIKQIsDYFPRFP
PRKCIP41743humanFBXW7Q969H0S18KRRRTGGsLRGNPSs
PRKCIP41743humanROCK1Q13464T1024IDRKKANtQDLRKKE
PRKCIP41743humanMAPTP10636-8S293NVQskCGsKDNIkHVTubulin-binding
PRKCIP41743humanBADQ92934S99PFrGrsRsAPPNLWABcl-2_BAD
PRKCIP41743humanUBTFP17480S412EkPKRPVsAMFIFSEHMG_box
PRKCIP41743humanELF3P78545S68GEQPQFWsKTQVLDWSAM_PNT
PRKCIP41743humanPARD6AQ9NPB6S345RGDGSGFsL______
PRKCIP41743humanECT2Q9H8V3T359YLYEKANtPELKksV
PRKCIP41743humanAMOTQ4VCS5T536GSEDTRKtIsQLFAK
PRKCIP41743humanGLI1P08151T304GEKPHKCtFEGCRKSzf-C2H2
PRKCIP41743humanBADQ92934S75EIRsRHssyPAGtEDBcl-2_BAD
PRKCIP41743humanVIMP08670S34srsyVttstrtysLGFilament_head
PRKCIP41743humanKLF10Q13118S384GkTYFKssHLkAHTR
PRKCIP41743humanMAPTP10636-8S258PDLkNVKskIGstENTubulin-binding
PRKCIP41743humanEZH2Q15910T487APAEDVDtPPRKKKR
PRKCIP41743humanEZH2Q15910S690KIRFANHsVNPNCyASET
PRKCIP41743humanCTTNQ14247S261EKLQLHEsQkDyktGHS1_rep
PRKCIP41743humanRPS6KB2Q9UBS0S473PPSGTKKsKRGRGRP
PRKCIP41743humanBADQ92934S118GRELRRMsDEFVDsFBcl-2_BAD
PRKCIP41743humanYKT6O15498S174LDDLVsksEVLGtQSSynaptobrevin
PRKCIP41743humanVIMP08670S39ttstrtysLGsALrPFilament_head
PRKCIP41743humanROCK1Q13464S1341tRstANQsFRKVVKN
PRKCIP41743humanAMOTQ4VCS5S538EDTRKtIsQLFAKNk
PRKCIP41743humanEP300Q09472S89SELLRSGsSPNLNMG
PRKCIP41743humanPARD3Q8TEW0S827REGFGRQsMSEKRtK
PRKCIP41743humanKLF10Q13118T445FMRSDHLtkHARRHLzf-C2H2
PRKCIP41743humanEZH2Q15910T345LtAERIKtPPkRPGG
PRKCIP41743humanNFE2L2Q16236S40SREVFDFsQRRkEYE
PRKCIP41743humanVIMP08670S56srsLyAssPGGVyAtFilament_head


check button Biological Network Integration of This Kinase and Substrates
(GeneMANIA website)

check button Enriched GO biological processes of the phosphorylation target genes of the kinase
KinaseGOIDGO termP.adjust
PRKCIIDDescription0.00e+00
PRKCIGO:1903829positive regulation of protein localization3.05e-04
PRKCIGO:0010821regulation of mitochondrion organization1.51e-03
PRKCIGO:0051222positive regulation of protein transport1.81e-03
PRKCIGO:1904951positive regulation of establishment of protein localization1.81e-03
PRKCIGO:0033674positive regulation of kinase activity1.81e-03
PRKCIGO:0010506regulation of autophagy2.04e-03
PRKCIGO:0045216cell-cell junction organization2.28e-03
PRKCIGO:0007163establishment or maintenance of cell polarity2.71e-03
PRKCIGO:0051347positive regulation of transferase activity3.24e-03
PRKCIGO:0030866cortical actin cytoskeleton organization3.92e-03
PRKCIGO:0010975regulation of neuron projection development4.40e-03
PRKCIGO:0030865cortical cytoskeleton organization5.18e-03
PRKCIGO:0048511rhythmic process5.18e-03
PRKCIGO:0045197establishment or maintenance of epithelial cell apical/basal polarity5.18e-03
PRKCIGO:0034614cellular response to reactive oxygen species5.18e-03
PRKCIGO:0030010establishment of cell polarity5.18e-03
PRKCIGO:0010976positive regulation of neuron projection development5.18e-03
PRKCIGO:0035088establishment or maintenance of apical/basal cell polarity5.18e-03
PRKCIGO:0061245establishment or maintenance of bipolar cell polarity5.18e-03
PRKCIGO:0062197cellular response to chemical stress5.24e-03
PRKCIGO:0051660establishment of centrosome localization5.69e-03
PRKCIGO:0034250positive regulation of amide metabolic process5.72e-03
PRKCIGO:0071496cellular response to external stimulus6.56e-03
PRKCIGO:0070301cellular response to hydrogen peroxide6.56e-03
PRKCIGO:0043534blood vessel endothelial cell migration6.56e-03
PRKCIGO:0070830bicellular tight junction assembly6.61e-03
PRKCIGO:0031346positive regulation of cell projection organization6.61e-03
PRKCIGO:0010631epithelial cell migration7.44e-03
PRKCIGO:0120192tight junction assembly7.44e-03
PRKCIGO:0090132epithelium migration7.44e-03
PRKCIGO:0043297apical junction assembly7.44e-03
PRKCIGO:0090130tissue migration7.44e-03
PRKCIGO:0032388positive regulation of intracellular transport7.44e-03
PRKCIGO:0007623circadian rhythm7.56e-03
PRKCIGO:0000302response to reactive oxygen species7.56e-03
PRKCIGO:0120193tight junction organization7.70e-03
PRKCIGO:1902806regulation of cell cycle G1/S phase transition7.70e-03
PRKCIGO:0006979response to oxidative stress7.73e-03
PRKCIGO:0002064epithelial cell development7.73e-03
PRKCIGO:1900034regulation of cellular response to heat7.73e-03
PRKCIGO:2000345regulation of hepatocyte proliferation7.73e-03
PRKCIGO:0043923positive regulation by host of viral transcription8.54e-03
PRKCIGO:0022406membrane docking9.50e-03
PRKCIGO:0000422autophagy of mitochondrion1.09e-02
PRKCIGO:0061726mitochondrion disassembly1.09e-02
PRKCIGO:0018107peptidyl-threonine phosphorylation1.12e-02
PRKCIGO:0007409axonogenesis1.12e-02
PRKCIGO:0042542response to hydrogen peroxide1.12e-02
PRKCIGO:0090043regulation of tubulin deacetylation1.13e-02

Top

Related Drugs to ACTG2_PRKCI


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

check button Distribution of the number of studies mentioning ACTG2-PRKCI and kinase inhibitors the PubMed Abstract (04-01-2024)

Fusion gene - drug pair 1Fusion gene - drug pair 2PMIDPublication dateDOIStudy title

Top

Related Diseases to ACTG2_PRKCI


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Related diseases from the literature mentioned this fusion gene and drug.
(PubMed, 04-01-2024)
MeSH IDMeSH term

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource


Top

Clinical Trials of the Found Drugs/Small Molecules


check button Statistics of the Clinical Trials of the Found Kinase Inibitors from clinicaltrials.gov (06-17-2024)

check button Clinical Trials from clinicaltrials.gov (06-17-2024)

Fusion GeneKinase InhibitorNCT IDStudy StatusPhasesDisease# EnrolmentDate