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	Kinase Fusion Gene Summary
	Kinase Fusion Gene Sample Information
	Kinase Fusion ORF Analysis
	Kinase Fusion Amino Acid Sequences
	Multiple Sequence Alignment of All Fusion Protein Isoforms
	Kinase Fusion Protein Functional Features
	Kinase Fusion Protein Structures
	Comparison of Fusion Protein Isoforms
	Comparison of Fusion Protein Sequences/Structures with Known Sequences/Structures from PDB
	pLDDT Scores and Difference Analysis of pLDDT Scores Between the Active Sites (Best) and Non-Active Sites.
	Ramachandran Plot of Kinase Fusion Protein Structure
	Potential Active Site Information
	Virtual Screening Results
	Kinase-Substrate Information
	Related Drugs with This Kinase Fusion Protein
	Related Disease with This Kinase Fusion Protein
	Clinical Trials of the Found Drugs/Small Molecules

Kinase Fusion Gene:AAK1_WFDC13

Kinase Fusion Protein Summary

Kinase Fusion gene summary

Kinase Fusion partner gene information	Kinase Fusion gene name: AAK1_WFDC13
	KinaseFusionDB ID: KFG23
	FusionGDB2.0 ID: KFG23
		Hgene	Tgene
	Gene symbol	AAK1	WFDC13
	Gene ID	22848	164237
	Gene name	AP2 associated kinase 1	WAP four-disulfide core domain 13
	Synonyms	-	C20orf138\|WAP13\|dJ601O1.3
	Cytomap	2p13.3	20q13.12
	Type of gene	protein-coding	protein-coding
	Description	AP2-associated protein kinase 1adaptor-associated kinase 1alternative protein AAK1	WAP four-disulfide core domain protein 13protease inhibitor WAP13protein WFDC13
	Modification date	20240403	20240305
	UniProtAcc	Q2M2I8	Q8IUB5
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000406297, ENST00000409085, ENST00000409068, ENST00000470281,	ENST00000305479,
Context (manual curation of fusion genes in KinaseFusionDB)	PubMed: AAK1 [Title/Abstract] AND WFDC13 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	AAK1(69732701)-WFDC13(44333083), # samples:3

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	AAK1	GO:0006468	protein phosphorylation	17494869\|18657069
Hgene	AAK1	GO:0032880	regulation of protein localization	18657069
Hgene	AAK1	GO:0045747	positive regulation of Notch signaling pathway	21464124
Hgene	AAK1	GO:0050821	protein stabilization	21464124
Hgene	AAK1	GO:2000369	regulation of clathrin-dependent endocytosis	18657069

Kinase Fusion gene breakpoints across AAK1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Kinase Fusion gene breakpoints across WFDC13 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Kinase Fusion Gene Sample Information

Kinase Fusion gene information.

Kinase Fusion gene information from four resources (ChiTars 5.0, ChimerDB 4.0, COSMIC, and CCLE)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Sample	Hgene	Hchr	Hbp	Tgene	Tchr	Tbp
ChimerDB4	TCGA-22-A5C4-01A	AAK1	chr2	69732701	WFDC13	chr20	44333083
ChimerDB4	TCGA-22-A5C4	AAK1	chr2	69723117	WFDC13	chr20	44333083

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Kinase Fusion ORF Analysis

Kinase Fusion information from ORFfinder translation from full-length transcript sequence from KinaseFusionDB.

Henst	Tenst	Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Seq length (transcript)	Seq length (amino acids)
ENST00000409085	ENST00000305479	AAK1	chr2	69732701	WFDC13	chr20	44333083	3822	853
ENST00000409085	ENST00000305479	AAK1	chr2	69723117	WFDC13	chr20	44333083	3918	885

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Kinase Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from KinaseFusionDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>Henst_Tenst_Hgene_Hchr_Hbp_Tgene_Tchr_Tbp_length(fusion AA)_AAseq

>ENST00000409085_ENST00000305479_AAK1_chr2_69732701_WFDC13_chr20_44333083_length(amino acids)=853
MRRNPWISFLKKKKKKKKSRNHRYFALLLPIRKMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGF
AIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFT
ENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGK
IITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNS
PIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQ
PKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPAT
QKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPV
RQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVY
NPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTEYILEPPPCISAPENCTHLCT

--------------------------------------------------------------

>ENST00000409085_ENST00000305479_AAK1_chr2_69723117_WFDC13_chr20_44333083_length(amino acids)=885
MRRNPWISFLKKKKKKKKSRNHRYFALLLPIRKMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGF
AIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFT
ENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGK
IITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNS
PIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQ
PKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPAT
QKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPV
RQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVY
NPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSD

--------------------------------------------------------------

Multiple Sequence Alignment of All Fusion Protein Isoforms

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Kinase Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:69732701/chr20:44333083)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
AAK1 Q2M2I8	WFDC13 Q8IUB5
FUNCTION: Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:17494869, PubMed:11877457, PubMed:11877461, PubMed:12952931, PubMed:14617351, PubMed:25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed:17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed:11877457, PubMed:18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed:12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed:18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed:21464124). {ECO:0000269\|PubMed:11877457, ECO:0000269\|PubMed:11877461, ECO:0000269\|PubMed:12952931, ECO:0000269\|PubMed:14617351, ECO:0000269\|PubMed:17494869, ECO:0000269\|PubMed:18657069, ECO:0000269\|PubMed:21464124, ECO:0000269\|PubMed:25653444}.; FUNCTION: (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue. {ECO:0000269\|PubMed:25653444, ECO:0000305\|PubMed:31136173}.	FUNCTION: Putative acid-stable proteinase inhibitor. {ECO:0000250}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained domain in the 5'-partner of fusion protein (protein functional feature from UniProt).

788788756756

Partner

Hgeneene

Hbp

Tgeneene

Tbp

ENST

BPexon

TotalExon

Protein feature loci

BPloci

TotalLen

Feature

Note

Hgene

AAK1

69723117

WFDC13

44333083

ENST00000409085

46_315

864

Domain

Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159

Hgene

AAK1

69723117

WFDC13

44333083

ENST00000409085

46_315

962

Domain

Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159

Hgene

AAK1

69732701

WFDC13

44333083

ENST00000409085

46_315

864

Domain

Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159

Hgene

AAK1

69732701

WFDC13

44333083

ENST00000409085

46_315

962

Domain

Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159

- Retained domain in the 3'-partner of fusion protein (protein functional feature from UniProt).

Partner

Hgeneene

Hbp

Tgeneene

Tbp

ENST

BPexon

TotalExon

Protein feature loci

BPloci

TotalLen

Feature

Note

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Kinase Fusion Protein Structures

CIF files of the predicted kinase fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Kinase Fusion protein CIF link (fusion AA seq ID in KinaseFusionDB)	Henst	Tenst	Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	AA seq	Len(AA seq)
PDB file >>>3_AAK1_WFDC13	ENST00000409085	ENST00000305479	AAK1	chr2	69723117	WFDC13	chr20	44333083	MRRNPWISFLKKKKKKKKSRNHRYFALLLPIRKMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGF AIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFT ENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGK IITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNS PIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQ PKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPAT QKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPV RQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVY NPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSD	885
3D view using mol* of 3_AAK1_WFDC13

PDB file >>>HKFP_3_AAK1_WFDC13	ENST00000409085	ENST00000305479	AAK1	chr2	69732701	WFDC13	chr20	44333083	MRRNPWISFLKKKKKKKKSRNHRYFALLLPIRKMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGF AIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFT ENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGK IITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNS PIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQ PKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPAT QKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPV RQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVY NPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTEYILEPPPCISAPENCTHLCT	853_AAK1_WFDC13
PDB file >>>HKFP_4_AAK1_WFDC13	ENST00000409085	ENST00000305479	AAK1	chr2	69723117	WFDC13	chr20	44333083	MRRNPWISFLKKKKKKKKSRNHRYFALLLPIRKMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGF AIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFT ENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGK IITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNS PIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQ PKPQAPPSQPLPQTQAKQPQAPPTPQQTPSTQAQGLPAQAQATPQHQQQLFLKQQQQQQQPPPAQQQPAGTFYQQQQAQTQQFQAVHPAT QKPAIAQFPVVSQGGSQQQLMQNFYQQQQQQQQQQQQQQLATALHQQQLMTQQAALQQKPTMAAGQQPQPQPAAAPQPAPAQEPAIQAPV RQQPKVQTTPPPAVQGQKVGSLTPPSSPKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVY NPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSD	885_AAK1_WFDC13
3D view using mol* of HKFP_4_AAK1_WFDC13

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Comparison of Fusion Protein Isoforms

Superimpose the 3D Structures Among All Fusion Protein Isoforms
* Download the pdb file and open it from the molstar online viewer.

3D view using mol* of viewer/superimpose_isoforms/HKFP_3_AAK1_WFDC13_vs_HKFP_4_AAK1_WFDC13_superimposed.pdb.html

Comparison of the Secondary Structures of Fusion Protein Isoforms

./secondary_str/HKFP_4_AAK1_WFDC13_vs_HKFP_3_AAK1_WFDC13.png

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Comparison of Fusion Protein Sequences/Structures with Known Sequences/Structures from PDB

AAK1_WFDC13 does not have any known PDB structures.

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pLDDT score distribution

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
* The blue color at the bottom marks the best active site residues.

3_AAK1_WFDC13.png

HKFP_4_AAK1_WFDC13.png

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Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Kinase Fusion AA seq ID in KinaseFusionDB	Site score	Size	Dscore	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
HKFP_4_AAK1_WFDC13	1.103	123	1.176	415.716	0.47	0.73	0.991	1.528	0.618	2.474	1.574	Chain A: 6,10,13,14,16,17,20,24,171,174,175,176,17 7,178,289,290,291,310,312,313,314,315

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Ramachandran Plot of Kinase Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

3_AAK1_WFDC13_ramachandran.png

HKFP_4_AAK1_WFDC13_ramachandran.png

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Virtual Screening Results

Distribution of the average docking score across all approved kinase inhibitors.
Distribution of the number of occurrence across all approved kinase inhibitors.

5'-kinase fusion protein case

3'-kinase fusion protein case

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Drug information from DrugBank of the top 20 interacting small molecules.
* The detailed information of individual kinase inhibitors are available in the download page.

Fusion gene name info	Drug	Docking score	Glide g score	Glide energy
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Dasatinib	-7.42678	-7.91268	-55.7435
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Dasatinib	-7.42678	-7.91268	-55.7435
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Dasatinib	-7.42678	-7.91268	-55.7435
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Dasatinib	-7.01934	-7.517639999999999	-53.6785
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Dasatinib	-7.01934	-7.517639999999999	-53.6785
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Dasatinib	-7.01934	-7.517639999999999	-53.6785
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Pexidartinib	-6.8666399999999985	-7.08344	-44.7417
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Pexidartinib	-6.8666399999999985	-7.08344	-44.7417
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Tofacitinib	-6.7443	-6.7558	-40.552
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Tofacitinib	-6.7443	-6.7558	-40.552
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Cobimetinib	-6.54502	-6.54782	-41.0212
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Gilteritinib	-6.43991	-6.46631	-44.065
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Gilteritinib	-6.43991	-6.46631	-44.065
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Gilteritinib	-6.43991	-6.46631	-44.065
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Gilteritinib	-6.43991	-6.46631	-44.065
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Asciminib	-6.30022	-6.66332	-49.9269
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Tofacitinib	-6.29202	-6.30352	-28.8859
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Tofacitinib	-6.29202	-6.30352	-28.8859
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Fostamatinib	-6.27007	-6.33367	-50.1195
HKFP_4_AAK1_WFDC13_vsw_1-DOCK_HTVS_1-001	Fostamatinib	-6.27007	-6.33367	-50.1195
3_AAK1_WFDC13-DOCK_HTVS_1-001	Selpercatinib	-9.41287	-9.44337	-58.7827
3_AAK1_WFDC13-DOCK_HTVS_1-001	Selpercatinib	-9.41287	-9.44337	-58.7827
3_AAK1_WFDC13-DOCK_HTVS_1-001	Tepotinib	-8.384830000000001	-8.38593	-58.6908
3_AAK1_WFDC13-DOCK_HTVS_1-001	Nilotinib	-8.32788	-8.46748	-56.0882
3_AAK1_WFDC13-DOCK_HTVS_1-001	Nilotinib	-8.32788	-8.46748	-56.0882
3_AAK1_WFDC13-DOCK_HTVS_1-001	Selpercatinib	-8.097539999999999	-8.128039999999999	-66.2529
3_AAK1_WFDC13-DOCK_HTVS_1-001	Acalabrutinib	-7.7868699999999995	-7.8009699999999995	-38.2538
3_AAK1_WFDC13-DOCK_HTVS_1-001	Acalabrutinib	-7.7868699999999995	-7.8009699999999995	-38.2538
3_AAK1_WFDC13-DOCK_HTVS_1-001	Selpercatinib	-7.634810000000001	-7.665310000000001	-35.9029
3_AAK1_WFDC13-DOCK_HTVS_1-001	Pexidartinib	-7.401439999999999	-7.618239999999999	-46.1783
3_AAK1_WFDC13-DOCK_HTVS_1-001	Pexidartinib	-7.401439999999999	-7.618239999999999	-46.1783
3_AAK1_WFDC13-DOCK_HTVS_1-001	Avapritinib	-7.3163100000000005	-8.59521	-56.589
3_AAK1_WFDC13-DOCK_HTVS_1-001	Avapritinib	-7.3163100000000005	-8.59521	-56.589
3_AAK1_WFDC13-DOCK_HTVS_1-001	Avapritinib	-7.3163100000000005	-8.59521	-56.589
3_AAK1_WFDC13-DOCK_HTVS_1-001	Avapritinib	-7.3163100000000005	-8.59521	-56.589
3_AAK1_WFDC13-DOCK_HTVS_1-001	Pazopanib	-7.221839999999999	-7.228739999999999	-47.2839
3_AAK1_WFDC13-DOCK_HTVS_1-001	Pazopanib	-7.221839999999999	-7.228739999999999	-47.2839
3_AAK1_WFDC13-DOCK_HTVS_1-001	Osimertinib	-7.1834	-7.1911	-53.0904
3_AAK1_WFDC13-DOCK_HTVS_1-001	Nilotinib	-6.973910000000001	-7.89871	-60.3933
3_AAK1_WFDC13-DOCK_HTVS_1-001	Nilotinib	-6.973910000000001	-7.89871	-60.3933

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Kinase-Substrate Information of AAK1_WFDC13

Phosphorylation target of the kinase
(phosphosite, 03-17-2024)

Kinase	Kinase UniProt Acc	Kinase species	Substrate	Substrate UniProt Acc	Substrate phosphorylated residues	Substrate phosphorylated sites (+/-7AA)	Domain
AAK1	Q2M2I8	human	AP2M1	Q96CW1	T156	SQItsQVtGQIGWRR
AAK1	Q2M2I8	human	NUMB	P49757	T102	LRVVDEKtKDLIVDQ	PID

Biological Network Integration of This Kinase and Substrates
(GeneMANIA website)

Enriched GO biological processes of the phosphorylation target genes of the kinase

Kinase	GOID	GO term	P.adjust
AAK1	ID	Description	0.00e+00
AAK1	GO:0098884	postsynaptic neurotransmitter receptor internalization	2.66e-05
AAK1	GO:0140239	postsynaptic endocytosis	2.66e-05
AAK1	GO:1903077	negative regulation of protein localization to plasma membrane	2.66e-05
AAK1	GO:1904376	negative regulation of protein localization to cell periphery	2.66e-05
AAK1	GO:0099590	neurotransmitter receptor internalization	2.66e-05
AAK1	GO:1905476	negative regulation of protein localization to membrane	3.18e-05
AAK1	GO:0002090	regulation of receptor internalization	7.99e-05
AAK1	GO:0099072	regulation of postsynaptic membrane neurotransmitter receptor levels	1.37e-04
AAK1	GO:1903076	regulation of protein localization to plasma membrane	1.84e-04
AAK1	GO:0048259	regulation of receptor-mediated endocytosis	1.88e-04
AAK1	GO:0031623	receptor internalization	2.12e-04
AAK1	GO:1904375	regulation of protein localization to cell periphery	2.22e-04
AAK1	GO:1905475	regulation of protein localization to membrane	3.59e-04
AAK1	GO:0031503	protein-containing complex localization	3.60e-04
AAK1	GO:1903828	negative regulation of protein localization	4.31e-04
AAK1	GO:0099003	vesicle-mediated transport in synapse	4.31e-04
AAK1	GO:0006898	receptor-mediated endocytosis	5.59e-04
AAK1	GO:0072659	protein localization to plasma membrane	6.36e-04
AAK1	GO:0030100	regulation of endocytosis	6.36e-04
AAK1	GO:1990778	protein localization to cell periphery	8.90e-04
AAK1	GO:0021670	lateral ventricle development	3.09e-03
AAK1	GO:0055057	neuroblast division	3.44e-03
AAK1	GO:0099149	regulation of postsynaptic neurotransmitter receptor internalization	3.76e-03
AAK1	GO:0097494	regulation of vesicle size	4.11e-03
AAK1	GO:1900242	regulation of synaptic vesicle endocytosis	4.11e-03
AAK1	GO:1903421	regulation of synaptic vesicle recycling	5.21e-03
AAK1	GO:0002092	positive regulation of receptor internalization	5.21e-03
AAK1	GO:0021846	cell proliferation in forebrain	5.21e-03
AAK1	GO:0021591	ventricular system development	6.15e-03
AAK1	GO:0072583	clathrin-dependent endocytosis	8.46e-03
AAK1	GO:0048260	positive regulation of receptor-mediated endocytosis	9.23e-03
AAK1	GO:0034332	adherens junction organization	9.61e-03
AAK1	GO:0048488	synaptic vesicle endocytosis	1.14e-02
AAK1	GO:0140238	presynaptic endocytosis	1.14e-02
AAK1	GO:0007405	neuroblast proliferation	1.22e-02
AAK1	GO:0036465	synaptic vesicle recycling	1.22e-02
AAK1	GO:0006900	vesicle budding from membrane	1.22e-02
AAK1	GO:0045807	positive regulation of endocytosis	2.20e-02
AAK1	GO:0061351	neural precursor cell proliferation	2.29e-02
AAK1	GO:0099504	synaptic vesicle cycle	2.66e-02
AAK1	GO:0045216	cell-cell junction organization	2.81e-02
AAK1	GO:0050769	positive regulation of neurogenesis	3.09e-02
AAK1	GO:0021537	telencephalon development	3.44e-02
AAK1	GO:0051962	positive regulation of nervous system development	3.54e-02
AAK1	GO:0016050	vesicle organization	4.31e-02
AAK1	GO:0032535	regulation of cellular component size	4.31e-02
AAK1	GO:0050767	regulation of neurogenesis	4.44e-02
AAK1	GO:0030900	forebrain development	4.53e-02
AAK1	GO:0010720	positive regulation of cell development	4.79e-02

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Related Drugs to AAK1_WFDC13

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Distribution of the number of studies mentioning AAK1-WFDC13 and kinase inhibitors the PubMed Abstract (04-01-2024)

Fusion gene - drug pair 1

Fusion gene - drug pair 2

PMID

Publication date

DOI

Study title

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Related Diseases to AAK1_WFDC13

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Related diseases from the literature mentioned this fusion gene and drug.
(PubMed, 04-01-2024)

MeSH ID

MeSH term

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

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Clinical Trials of the Found Drugs/Small Molecules

Statistics of the Clinical Trials of the Found Kinase Inibitors from clinicaltrials.gov (06-17-2024)

Clinical Trials from clinicaltrials.gov (06-17-2024)

Fusion Gene

Kinase Inhibitor

NCT ID

Study Status

Phases

Disease

# Enrolment

Date