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	Kinase Fusion Gene Summary
	Kinase Fusion Gene Sample Information
	Kinase Fusion ORF Analysis
	Kinase Fusion Amino Acid Sequences
	Multiple Sequence Alignment of All Fusion Protein Isoforms
	Kinase Fusion Protein Functional Features
	Kinase Fusion Protein Structures
	Comparison of Fusion Protein Isoforms
	Comparison of Fusion Protein Sequences/Structures with Known Sequences/Structures from PDB
	pLDDT Scores and Difference Analysis of pLDDT Scores Between the Active Sites (Best) and Non-Active Sites.
	Ramachandran Plot of Kinase Fusion Protein Structure
	Potential Active Site Information
	Virtual Screening Results
	Kinase-Substrate Information
	Related Drugs with This Kinase Fusion Protein
	Related Disease with This Kinase Fusion Protein
	Clinical Trials of the Found Drugs/Small Molecules

Kinase Fusion Gene:KLC1_MARK3

Kinase Fusion Protein Summary

Kinase Fusion gene summary

Kinase Fusion partner gene information	Kinase Fusion gene name: KLC1_MARK3
	KinaseFusionDB ID: KFG3051
	FusionGDB2.0 ID: KFG3051
		Hgene	Tgene
	Gene symbol	KLC1	MARK3
	Gene ID	3831	4140
	Gene name	kinesin light chain 1	microtubule affinity regulating kinase 3
	Synonyms	KLC\|KNS2\|KNS2A	CTAK1\|KP78\|PAR1A\|Par-1a\|VIPB
	Cytomap	14q32.33	14q32.32-q32.33
	Type of gene	protein-coding	protein-coding
	Description	kinesin light chain 1kinesin 2 60/70kDamedulloblastoma antigen MU-MB-2.50	MAP/microtubule affinity-regulating kinase 3C-TAK1ELKL motif kinase 2EMK-2cdc25C-associated protein kinase 1protein kinase STK10ser/Thr protein kinase PAR-1serine/threonine-protein kinase p78
	Modification date	20240305	20240407
	UniProtAcc	Q07866	P27448
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000246489, ENST00000334553, ENST00000347839, ENST00000348520, ENST00000380038, ENST00000389744, ENST00000445352, ENST00000452929, ENST00000553286, ENST00000554280, ENST00000555836, ENST00000557450, ENST00000557575,	ENST00000561071, ENST00000216288, ENST00000303622, ENST00000335102, ENST00000416682, ENST00000429436, ENST00000440884, ENST00000553942,
Context (manual curation of fusion genes in KinaseFusionDB)	PubMed: KLC1 [Title/Abstract] AND MARK3 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	KLC1(104095831)-MARK3(103969219), # samples:3

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	MARK3	GO:0018105	peptidyl-serine phosphorylation	9543386
Tgene	MARK3	GO:0032092	positive regulation of protein binding	9543386
Tgene	MARK3	GO:0035331	negative regulation of hippo signaling	28087714
Tgene	MARK3	GO:0036289	peptidyl-serine autophosphorylation	9543386
Tgene	MARK3	GO:1900181	negative regulation of protein localization to nucleus	16822840

Kinase Fusion gene breakpoints across KLC1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Kinase Fusion gene breakpoints across MARK3 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Kinase Fusion Gene Sample Information

Kinase Fusion gene information.

Kinase Fusion gene information from four resources (ChiTars 5.0, ChimerDB 4.0, COSMIC, and CCLE)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Sample	Hgene	Hchr	Hbp	Tgene	Tchr	Tbp
ChimerDB4	TCGA-B5-A3S1-01A	KLC1	chr14	104124113	MARK3	chr14	103969219
ChimerDB4	TCGA-D8-A1JK-01A	KLC1	chr14	104136620	MARK3	chr14	103915256
ChimerDB4	TCGA-D8-A1JK	KLC1	chr14	104136620	MARK3	chr14	103915255
ChimerDB4	TCGA-B5-A0JN-01A	KLC1	chr14	104095831	MARK3	chr14	103969219
ChimerDB4	TCGA-B5-A0JN	KLC1	chr14	104095831	MARK3	chr14	103969218
CCLE	TO14	KLC1	chr14	104145882	MARK3	chr14	103969219
CCLE	TO14	KLC1	chr14	104145855	MARK3	chr14	103969219

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Kinase Fusion ORF Analysis

Kinase Fusion information from ORFfinder translation from full-length transcript sequence from KinaseFusionDB.

Henst	Tenst	Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Seq length (transcript)	Seq length (amino acids)
ENST00000348520	ENST00000553942	KLC1	chr14	104145882	MARK3	chr14	103969219	2333	555
ENST00000348520	ENST00000553942	KLC1	chr14	104145855	MARK3	chr14	103969219	2198	510
ENST00000348520	ENST00000440884	KLC1	chr14	104136620	MARK3	chr14	103915256	3014	889
ENST00000348520	ENST00000553942	KLC1	chr14	104124113	MARK3	chr14	103969219	1175	169
ENST00000348520	ENST00000440884	KLC1	chr14	104136620	MARK3	chr14	103915255	3014	889

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Kinase Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from KinaseFusionDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>Henst_Tenst_Hgene_Hchr_Hbp_Tgene_Tchr_Tbp_length(fusion AA)_AAseq

>ENST00000348520_ENST00000553942_KLC1_chr14_104145882_MARK3_chr14_103969219_length(amino acids)=555
MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM
ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF
PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK
YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEY
YYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYG
GWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGE

--------------------------------------------------------------

>ENST00000348520_ENST00000553942_KLC1_chr14_104145855_MARK3_chr14_103969219_length(amino acids)=510
MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM
ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF
PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK
YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEY
YYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYG

--------------------------------------------------------------

>ENST00000348520_ENST00000440884_KLC1_chr14_104136620_MARK3_chr14_103915256_length(amino acids)=889
MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM
ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF
PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK
YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKLFREVRIMKILNHPNIVKLFEVIETEKTLYL
IMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLE
QIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRP
SSDLNNSTGQSPHHKVQRSVFSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPN
KADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQP
RERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGD

--------------------------------------------------------------

>ENST00000348520_ENST00000553942_KLC1_chr14_104124113_MARK3_chr14_103969219_length(amino acids)=169
MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM

--------------------------------------------------------------

>ENST00000348520_ENST00000440884_KLC1_chr14_104136620_MARK3_chr14_103915255_length(amino acids)=889
MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM
ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF
PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK
YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKLFREVRIMKILNHPNIVKLFEVIETEKTLYL
IMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLE
QIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRP
SSDLNNSTGQSPHHKVQRSVFSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPN
KADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQP
RERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGD

--------------------------------------------------------------

Multiple Sequence Alignment of All Fusion Protein Isoforms

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Kinase Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:104095831/chr14:103969219)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
KLC1 Q07866	MARK3 P27448
FUNCTION: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport (PubMed:21385839). The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity (By similarity). {ECO:0000250\|UniProtKB:P37285, ECO:0000269\|PubMed:21385839}.	FUNCTION: Serine/threonine-protein kinase (PubMed:16822840, PubMed:16980613, PubMed:23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Phosphorylates CDC25C on 'Ser-216' (PubMed:12941695). Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol (PubMed:16822840). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714). Phosphorylates PKP2 and KSR1 (PubMed:12941695). {ECO:0000269\|PubMed:12941695, ECO:0000269\|PubMed:16822840, ECO:0000269\|PubMed:16980613, ECO:0000269\|PubMed:23666762, ECO:0000269\|PubMed:28087714}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained domain in the 5'-partner of fusion protein (protein functional feature from UniProt).

Partner

Hgeneene

Hbp

Tgeneene

Tbp

ENST

BPexon

TotalExon

Protein feature loci

BPloci

TotalLen

Feature

Note

- Retained domain in the 3'-partner of fusion protein (protein functional feature from UniProt).

Partner	Hgeneene	Hbp	Tgeneene	Tbp	ENST	BPexon	TotalExon	Protein feature loci	BPloci	TotalLen	Feature	Note
Tgene	KLC1	104124113	MARK3	103969219	ENST00000348520	14	16	704_753	544	660	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104124113	MARK3	103969219	ENST00000348520	14	16	704_753	598	714	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104124113	MARK3	103969219	ENST00000348520	14	16	704_753	614	730	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104124113	MARK3	103969219	ENST00000348520	15	17	704_753	629	745	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104124113	MARK3	103969219	ENST00000348520	15	17	704_753	637	753	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104124113	MARK3	103969219	ENST00000348520	16	18	704_753	638	754	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	16	704_753	99	660	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	16	704_753	99	714	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	16	704_753	99	730	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	17	704_753	99	745	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	17	704_753	99	753	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	18	704_753	99	754	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	16	704_753	99	660	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	16	704_753	99	714	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	16	704_753	99	730	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	17	704_753	99	745	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	17	704_753	99	753	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	18	704_753	99	754	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145855	MARK3	103969219	ENST00000348520	14	16	704_753	544	660	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145855	MARK3	103969219	ENST00000348520	14	16	704_753	598	714	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145855	MARK3	103969219	ENST00000348520	14	16	704_753	614	730	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145855	MARK3	103969219	ENST00000348520	15	17	704_753	629	745	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145855	MARK3	103969219	ENST00000348520	15	17	704_753	637	753	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145855	MARK3	103969219	ENST00000348520	16	18	704_753	638	754	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145882	MARK3	103969219	ENST00000348520	14	16	704_753	544	660	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145882	MARK3	103969219	ENST00000348520	14	16	704_753	598	714	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145882	MARK3	103969219	ENST00000348520	14	16	704_753	614	730	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145882	MARK3	103969219	ENST00000348520	15	17	704_753	629	745	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145882	MARK3	103969219	ENST00000348520	15	17	704_753	637	753	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104145882	MARK3	103969219	ENST00000348520	16	18	704_753	638	754	Domain	Note=KA1;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00565
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	16	326_365	99	660	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	16	326_365	99	714	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	16	326_365	99	730	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	17	326_365	99	745	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	17	326_365	99	753	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915255	ENST00000348520	2	18	326_365	99	754	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	16	326_365	99	660	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	16	326_365	99	714	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	16	326_365	99	730	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	17	326_365	99	745	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	17	326_365	99	753	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212
Tgene	KLC1	104136620	MARK3	103915256	ENST00000348520	2	18	326_365	99	754	Domain	Note=UBA;Ontology_term=ECO:0000255;evidence=ECO:0000255\|PROSITE-ProRule:PRU00212

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Kinase Fusion Protein Structures

CIF files of the predicted kinase fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Kinase Fusion protein CIF link (fusion AA seq ID in KinaseFusionDB)	Henst	Tenst	Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	AA seq	Len(AA seq)
PDB file >>>271_KLC1_MARK3	ENST00000348520	ENST00000440884	KLC1	chr14	104136620	MARK3	chr14	103915255	MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKLFREVRIMKILNHPNIVKLFEVIETEKTLYL IMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLE QIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRP SSDLNNSTGQSPHHKVQRSVFSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPN KADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQP RERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGD	889
3D view using mol* of 271_KLC1_MARK3

PDB file >>>TKFP_478_KLC1_MARK3	ENST00000348520	ENST00000553942	KLC1	chr14	104145882	MARK3	chr14	103969219	MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEY YYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYG GWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPENMEKRRSRESLNVDVVKYESGPDGGE	555_KLC1_MARK3
PDB file >>>TKFP_479_KLC1_MARK3	ENST00000348520	ENST00000553942	KLC1	chr14	104145855	MARK3	chr14	103969219	MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEY YYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEREFGSVDDENKPIWMHAEEREECKGKQKDGTSFGEYG	510_KLC1_MARK3
3D view using mol* of TKFP_479_KLC1_MARK3

PDB file >>>TKFP_480_KLC1_MARK3	ENST00000348520	ENST00000440884	KLC1	chr14	104136620	MARK3	chr14	103915256	MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKLFREVRIMKILNHPNIVKLFEVIETEKTLYL IMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLE QIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRP SSDLNNSTGQSPHHKVQRSVFSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPN KADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQP RERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGD	889_KLC1_MARK3
3D view using mol* of TKFP_480_KLC1_MARK3

PDB file >>>TKFP_481_KLC1_MARK3	ENST00000348520	ENST00000553942	KLC1	chr14	104124113	MARK3	chr14	103969219	MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM	169_KLC1_MARK3
3D view using mol* of TKFP_481_KLC1_MARK3

PDB file >>>TKFP_482_KLC1_MARK3	ENST00000348520	ENST00000440884	KLC1	chr14	104136620	MARK3	chr14	103915255	MYDNMSTMVYIKEDKLEKLTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSNMIRKSLEMLELGLSEAQVMM ALSNHLNAVESEKQKLRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDTDSTKEPLDDLF PNDEDDPGQGIQQQHSSAAAAAQQGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNK YKDAANLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKLFREVRIMKILNHPNIVKLFEVIETEKTLYL IMEYASGGEVFDYLVAHGRMKEKEARSKFRQIVSAVQYCHQKRIVHRDLKELRERVLRGKYRIPFYMSTDCENLLKRFLVLNPIKRGTLE QIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLSKMKYDEITATYLLLGRKSSELDASDSSSSSNLSLAKVRP SSDLNNSTGQSPHHKVQRSVFSSQKQRRYSDHAGPAIPSVVAYPKRSQTSTADSDLKEDGISSRKSSGSAVGGKGIAPASPMLGNASNPN KADIPERKKSSTVPSSNTASGGMTRRNTYVCSERTTADRHSVIQNGKENSTIPDQRTPVASTHSISSAATPDRIRFPRGTASRSTFHGQP RERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRNMSFRFIKSRNVSAEQKDENKEAKPRSLRFTWSMKTTSSMDPGD	889_KLC1_MARK3

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Comparison of Fusion Protein Isoforms

Superimpose the 3D Structures Among All Fusion Protein Isoforms
* Download the pdb file and open it from the molstar online viewer.

3D view using mol* of viewer/superimpose_isoforms/TKFP_478_KLC1_MARK3_vs_TKFP_479_KLC1_MARK3_superimposed.pdb.html
3D view using mol* of viewer/superimpose_isoforms/TKFP_478_KLC1_MARK3_vs_TKFP_480_KLC1_MARK3_superimposed.pdb.html
3D view using mol* of viewer/superimpose_isoforms/TKFP_478_KLC1_MARK3_vs_TKFP_481_KLC1_MARK3_superimposed.pdb.html
3D view using mol* of viewer/superimpose_isoforms/TKFP_479_KLC1_MARK3_vs_TKFP_480_KLC1_MARK3_superimposed.pdb.html
3D view using mol* of viewer/superimpose_isoforms/TKFP_479_KLC1_MARK3_vs_TKFP_481_KLC1_MARK3_superimposed.pdb.html
3D view using mol* of viewer/superimpose_isoforms/TKFP_480_KLC1_MARK3_vs_TKFP_481_KLC1_MARK3_superimposed.pdb.html

Comparison of the Secondary Structures of Fusion Protein Isoforms

./secondary_str/TKFP_478_KLC1_MARK3_vs_TKFP_479_KLC1_MARK3.png

./secondary_str/TKFP_478_KLC1_MARK3_vs_TKFP_481_KLC1_MARK3.png

./secondary_str/TKFP_480_KLC1_MARK3_vs_TKFP_478_KLC1_MARK3.png

./secondary_str/TKFP_480_KLC1_MARK3_vs_TKFP_479_KLC1_MARK3.png

./secondary_str/TKFP_480_KLC1_MARK3_vs_TKFP_481_KLC1_MARK3.png

./secondary_str/TKFP_481_KLC1_MARK3_vs_TKFP_479_KLC1_MARK3.png

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Comparison of Fusion Protein Sequences/Structures with Known Sequences/Structures from PDB

KLC1_MARK3 does not have any known PDB structures.

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pLDDT score distribution

all_data/KinaseFusionDB_T_Results/KinaseFusionDB_T_ViolinPlots/271_KLC1_MARK3.pngall_data/KinaseFusionDB_T_Results/KinaseFusionDB_T_ViolinPlots/TKFP_479_KLC1_MARK3_updated_violin_plot.pngall_data/KinaseFusionDB_T_Results/KinaseFusionDB_T_ViolinPlots/TKFP_480_KLC1_MARK3_updated_violin_plot.pngall_data/KinaseFusionDB_T_Results/KinaseFusionDB_T_ViolinPlots/TKFP_481_KLC1_MARK3_updated_violin_plot.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
* The blue color at the bottom marks the best active site residues.

271_KLC1_MARK3.png

TKFP_479_KLC1_MARK3.png

TKFP_480_KLC1_MARK3.png

TKFP_481_KLC1_MARK3.png

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Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Kinase Fusion AA seq ID in KinaseFusionDB	Site score	Size	Dscore	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
TKFP_479_KLC1_MARK3	0.892	83	0.902	315.56	0.633	0.625	0.831	0.276	1.01	0.273	1.734	Chain A: 176,177,178,179,181,183,456,457,458,459,4 60,461,464,465,468,493,494,496,497,499,500,501,503 ,504,507
TKFP_480_KLC1_MARK3	1.034	325	1.087	1610.385	0.712	0.678	0.774	0.731	0.795	0.919	0.894	Chain A: 292,294,329,330,332,333,336,337,340,341,3 42,343,344,345,346,347,349,363,367,369,370,373,374 ,379,380,381,382,385,387,388,389,390,391,392,393,3 94,395,396,397,399,400,403,404,405,406,409,410,411 ,412,413,416,417,426,427,431,434,435,438,439,446,4 47,448,449,450,452,457,462,465,466,467,468,469,470 ,471,472,473,474,475,476,482,513,516,517,518,533,5 34,535,536,796,797
TKFP_481_KLC1_MARK3	0.642	14	0.672	40.817	0.745	0.569	0.697	2.226	0.063	35.337	0.991	Chain A: 21,24,25,89,92,93,96

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Ramachandran Plot of Kinase Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

271_KLC1_MARK3_ramachandran.png

TKFP_479_KLC1_MARK3_ramachandran.png

TKFP_480_KLC1_MARK3_ramachandran.png

TKFP_481_KLC1_MARK3_ramachandran.png

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Virtual Screening Results

Distribution of the average docking score across all approved kinase inhibitors.
Distribution of the number of occurrence across all approved kinase inhibitors.

5'-kinase fusion protein case

3'-kinase fusion protein case

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Drug information from DrugBank of the top 20 interacting small molecules.
* The detailed information of individual kinase inhibitors are available in the download page.

Fusion gene name info

Drug

Docking score

Glide g score

Glide energy

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Kinase-Substrate Information of KLC1_MARK3

Phosphorylation target of the kinase
(phosphosite, 03-17-2024)

Kinase	Kinase UniProt Acc	Kinase species	Substrate	Substrate UniProt Acc	Substrate phosphorylated residues	Substrate phosphorylated sites (+/-7AA)	Domain
MARK3	P27448-3	human	MARK3	P27448-3	S601	SQTRsRGstNLFsKL
MARK3	P27448-3	human	MARK3	P27448-3	S598	TPLSQTRsRGstNLF
MARK3	P27448-3	human	MARK3	P27448-3	S606	RGstNLFsKLTSKLT
MARK3	P27448-3	human	MARK3	P27448-3	T602	QTRsRGstNLFsKLT
MARK3	P27448	human	MEF2C	Q06413	S222	GYGNPRNsPGLLVsP
MARK3	P27448	human	PPP1R2	P41236	S72	LMKIDEPstPyHSMM	IPP-2
MARK3	P27448	human	CDC25C	P30307	S216	sGLyRsPsMPENLNR	M-inducer_phosp
MARK3	P27448	human	TNK1	Q13470	S502	RMKGIsRsLEsVLsL
MARK3	P27448	human	FEZ1	Q99689	S58	SEIISFKsMEDLVNE	FEZ
MARK3	P27448	human	ARHGEF2	Q92974	S151	LsLAksVsttNIAGH

Biological Network Integration of This Kinase and Substrates
(GeneMANIA website)

Enriched GO biological processes of the phosphorylation target genes of the kinase

Kinase

GOID

GO term

P.adjust

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Related Drugs to KLC1_MARK3

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Distribution of the number of studies mentioning KLC1-MARK3 and kinase inhibitors the PubMed Abstract (04-01-2024)

Fusion gene - drug pair 1

Fusion gene - drug pair 2

PMID

Publication date

DOI

Study title

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Related Diseases to KLC1_MARK3

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Related diseases from the literature mentioned this fusion gene and drug.
(PubMed, 04-01-2024)

MeSH ID

MeSH term

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

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Clinical Trials of the Found Drugs/Small Molecules

Statistics of the Clinical Trials of the Found Kinase Inibitors from clinicaltrials.gov (06-17-2024)

Clinical Trials from clinicaltrials.gov (06-17-2024)

Fusion Gene

Kinase Inhibitor

NCT ID

Study Status

Phases

Disease

# Enrolment

Date