| Kinase Fusion partner gene information | Kinase Fusion gene name: NEK3_CNOT1 |
KinaseFusionDB ID: KFG4129 | FusionGDB2.0 ID: KFG4129 | | Hgene | Tgene | Gene symbol | NEK3 | CNOT1 | Gene ID | 4752 | 23019 | | Gene name | NIMA related kinase 3 | CCR4-NOT transcription complex subunit 1 |
| Synonyms | HSPK36 | AD-005|CDC39|HPE12|NOT1|NOT1H|VIBOS |
| Cytomap | 13q14.3 | 16q21 |
| Type of gene | protein-coding | protein-coding |
| Description | serine/threonine-protein kinase Nek3HSPK 36NIMA (never in mitosis gene a)-related kinase 3glycogen synthase A kinasehydroxyalkyl-protein kinasenever in mitosis A-related kinase 3nimA-related protein kinase 3phosphorylase B kinase kinase | CCR4-NOT transcription complex subunit 1CCR4-associated factor 1NOT1 (negative regulator of transcription 1, yeast) homologadrenal gland protein AD-005negative regulator of transcription subunit 1 homolog |
| Modification date | 20240403 | 20240407 |
| UniProtAcc | P51956 | Q9H9A5 |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000339406, ENST00000378101,
ENST00000400357, ENST00000452082,
| ENST00000245138, ENST00000569732,
ENST00000317147, ENST00000441024,
ENST00000569240, |
| Context (manual curation of fusion genes in KinaseFusionDB) | PubMed: NEK3 [Title/Abstract] AND CNOT1 [Title/Abstract] AND fusion [Title/Abstract] |
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | NEK3(52730210)-CNOT1(58594266), # samples:1
|
 Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Kinase Fusion gene breakpoints across NEK3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Kinase Fusion gene breakpoints across CNOT1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Kinase Fusion gene information. |
Kinase Fusion gene information from four resources (ChiTars 5.0, ChimerDB 4.0, COSMIC, and CCLE)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Sample | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp |
| ChimerDB4 | TCGA-BR-7851-11A | NEK3 | chr13 | 52730210 | CNOT1 | chr16 | 58594266 |
| Kinase Fusion information from ORFfinder translation from full-length transcript sequence from KinaseFusionDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Seq length
(transcript) | Seq length
(amino acids) |
| For individual full-length fusion transcript sequence from KinaseFusionDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
>Henst_Tenst_Hgene_Hchr_Hbp_Tgene_Tchr_Tbp_length(fusion AA)_AAseq
|
Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:52730210/:58594266)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene. |
| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
NEK3
P51956 | CNOT1
Q9H9A5 |
| FUNCTION: Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. {ECO:0000269|PubMed:15618286, ECO:0000269|PubMed:17297458}. | FUNCTION: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex. {ECO:0000269|PubMed:23221646}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at download page * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained domain in the 5'-partner of fusion protein (protein functional feature from UniProt). |
| Partner | Hgeneene | Hbp | Tgeneene | Tbp | ENST | BPexon | TotalExon | Protein feature loci | BPloci | TotalLen | Feature | Note |
| - Retained domain in the 3'-partner of fusion protein (protein functional feature from UniProt). |
| Partner | Hgeneene | Hbp | Tgeneene | Tbp | ENST | BPexon | TotalExon | Protein feature loci | BPloci | TotalLen | Feature | Note |
Phosphorylation target of the kinase
(phosphosite, 03-17-2024) |
| Kinase | Kinase UniProt Acc | Kinase species | Substrate | Substrate UniProt Acc | Substrate phosphorylated residues | Substrate phosphorylated sites (+/-7AA) | Domain |
| NEK3 | P51956 | human | NEK3 | P51956 | T165 | FACTYVGtPYYVPPE | Pkinase |
| NEK3 | P51956 | human | SNAP29 | O95721 | S105 | MDQDLkIsQkHINsI | V-SNARE_C |
Biological Network Integration of This Kinase and Substrates
(GeneMANIA website) |
| Enriched GO biological processes of the phosphorylation target genes of the kinase |
| Kinase | GOID | GO term | P.adjust |
| NEK3 | ID | Description | 0.00e+00 |
| NEK3 | GO:0016082 | synaptic vesicle priming | 1.98e-02 |
| NEK3 | GO:0090043 | regulation of tubulin deacetylation | 1.98e-02 |
| NEK3 | GO:0031629 | synaptic vesicle fusion to presynaptic active zone membrane | 1.98e-02 |
| NEK3 | GO:0090042 | tubulin deacetylation | 1.98e-02 |
| NEK3 | GO:0099500 | vesicle fusion to plasma membrane | 1.98e-02 |
| NEK3 | GO:0048499 | synaptic vesicle membrane organization | 1.98e-02 |
| NEK3 | GO:0090311 | regulation of protein deacetylation | 2.12e-02 |
| NEK3 | GO:0006476 | protein deacetylation | 2.47e-02 |
| NEK3 | GO:0097352 | autophagosome maturation | 2.47e-02 |
| NEK3 | GO:0006903 | vesicle targeting | 2.47e-02 |
| NEK3 | GO:0035601 | protein deacylation | 2.47e-02 |
| NEK3 | GO:0098732 | macromolecule deacylation | 2.47e-02 |
| NEK3 | GO:0140029 | exocytic process | 2.48e-02 |
| NEK3 | GO:0140056 | organelle localization by membrane tethering | 2.48e-02 |
| NEK3 | GO:0022406 | membrane docking | 2.54e-02 |
| NEK3 | GO:0016079 | synaptic vesicle exocytosis | 2.54e-02 |
| NEK3 | GO:0006906 | vesicle fusion | 2.84e-02 |
| NEK3 | GO:0090174 | organelle membrane fusion | 2.84e-02 |
| NEK3 | GO:0007269 | neurotransmitter secretion | 3.03e-02 |
| NEK3 | GO:0099643 | signal release from synapse | 3.03e-02 |
| NEK3 | GO:0030010 | establishment of cell polarity | 3.03e-02 |
| NEK3 | GO:0048284 | organelle fusion | 3.03e-02 |
| NEK3 | GO:0061025 | membrane fusion | 3.10e-02 |
| NEK3 | GO:0001505 | regulation of neurotransmitter levels | 3.10e-02 |
| NEK3 | GO:0051650 | establishment of vesicle localization | 3.10e-02 |
| NEK3 | GO:0099504 | synaptic vesicle cycle | 3.10e-02 |
| NEK3 | GO:0006836 | neurotransmitter transport | 3.10e-02 |
| NEK3 | GO:0051648 | vesicle localization | 3.10e-02 |
| NEK3 | GO:0099003 | vesicle-mediated transport in synapse | 3.10e-02 |
| NEK3 | GO:0045055 | regulated exocytosis | 3.10e-02 |
| NEK3 | GO:0007163 | establishment or maintenance of cell polarity | 3.10e-02 |
| NEK3 | GO:0032984 | protein-containing complex disassembly | 3.22e-02 |
| NEK3 | GO:0016236 | macroautophagy | 4.22e-02 |
| NEK3 | GO:0006887 | exocytosis | 4.31e-02 |
| NEK3 | GO:0016050 | vesicle organization | 4.39e-02 |
| NEK3 | GO:0060271 | cilium assembly | 4.41e-02 |
| NEK3 | GO:0044782 | cilium organization | 4.61e-02 |
| NEK3 | GO:0051656 | establishment of organelle localization | 5.17e-02 |
| NEK3 | GO:0023061 | signal release | 5.19e-02 |
| NEK3 | GO:0022411 | cellular component disassembly | 5.20e-02 |
Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Distribution of the number of studies mentioning NEK3-CNOT1 and kinase inhibitors the PubMed Abstract (04-01-2024) |
| Fusion gene - drug pair 1 | Fusion gene - drug pair 2 | PMID | Publication date | DOI | Study title |
Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
Related diseases from the literature mentioned this fusion gene and drug.
(PubMed, 04-01-2024) |
Diseases associated with fusion partners.
(DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Statistics of the Clinical Trials of the Found Kinase Inibitors from clinicaltrials.gov (06-17-2024) |
| Clinical Trials from clinicaltrials.gov (06-17-2024) |
| Fusion Gene | Kinase Inhibitor | NCT ID | Study Status | Phases | Disease | # Enrolment | Date |
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