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Center for Computational Systems Medicine
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Kinase Fusion Gene Summary

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Kinase Fusion Gene Sample Information

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Kinase Fusion ORF Analysis

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Kinase Fusion Amino Acid Sequences

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Multiple Sequence Alignment of All Fusion Protein Isoforms

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Kinase Fusion Protein Functional Features

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Kinase Fusion Protein Structures

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Comparison of Fusion Protein Isoforms

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Comparison of Fusion Protein Sequences/Structures with Known Sequences/Structures from PDB

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pLDDT Scores and Difference Analysis of pLDDT Scores Between the Active Sites (Best) and Non-Active Sites.

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Ramachandran Plot of Kinase Fusion Protein Structure

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Potential Active Site Information

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Virtual Screening Results

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Kinase-Substrate Information

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Related Drugs with This Kinase Fusion Protein

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Related Disease with This Kinase Fusion Protein

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Clinical Trials of the Found Drugs/Small Molecules

Kinase Fusion Gene:PAK2_ANKUB1

Kinase Fusion Protein Summary

check button Kinase Fusion gene summary
Kinase Fusion partner gene informationKinase Fusion gene name: PAK2_ANKUB1
KinaseFusionDB ID: KFG4433
FusionGDB2.0 ID: KFG4433
HgeneTgene
Gene symbol

PAK2

ANKUB1

Gene ID

5586

389161

Gene nameprotein kinase N2ankyrin repeat and ubiquitin domain containing 1
SynonymsPAK2|PRK2|PRKCL2|PRO2042|Pak-2|STK7C3orf16
Cytomap

1p22.2

3q25.1

Type of geneprotein-codingprotein-coding
Descriptionserine/threonine-protein kinase N2PKN gammacardiolipin-activated protein kinase Pak2protein kinase C-like 2protein-kinase C-related kinase 2protein ANKUB1
Modification date2024032320240305
UniProtAcc

Q13177

A6NFN9

Ensembl transtripts involved in fusion geneENST idsENST00000327134, ENST00000473672, 
ENST00000383050, ENST00000446160, 
ENST00000462519, 
Context (manual curation of fusion genes in KinaseFusionDB)

PubMed: PAK2 [Title/Abstract] AND ANKUB1 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)PAK2(196547438)-ANKUB1(149569113), # samples:1
check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgenePAK2

GO:0006468

protein phosphorylation

17332740

HgenePAK2

GO:0010631

epithelial cell migration

21754995


check buttonKinase Fusion gene breakpoints across PAK2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

check buttonKinase Fusion gene breakpoints across ANKUB1 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.


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Kinase Fusion Gene Sample Information

check buttonKinase Fusion gene information.
check button Kinase Fusion gene information from four resources (ChiTars 5.0, ChimerDB 4.0, COSMIC, and CCLE)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceSampleHgeneHchrHbpTgeneTchrTbp
ChimerDB4TCGA-VS-A8EK-01APAK2chr3

196547438

ANKUB1chr3

149569113



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Kinase Fusion ORF Analysis


check buttonKinase Fusion information from ORFfinder translation from full-length transcript sequence from KinaseFusionDB.
HenstTenstHgeneHchrHbpTgeneTchrTbpSeq length
(transcript)		Seq length
(amino acids)

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Kinase Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from KinaseFusionDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>Henst_Tenst_Hgene_Hchr_Hbp_Tgene_Tchr_Tbp_length(fusion AA)_AAseq

Multiple Sequence Alignment of All Fusion Protein Isoforms



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Kinase Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:196547438/:149569113)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
PAK2

Q13177

ANKUB1

A6NFN9

FUNCTION: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation (PubMed:7744004, PubMed:19273597, PubMed:19923322, PubMed:9171063, PubMed:12853446, PubMed:16617111, PubMed:33693784). Acts as a downstream effector of the small GTPases CDC42 and RAC1 (PubMed:7744004). Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues (PubMed:7744004). Full-length PAK2 stimulates cell survival and cell growth (PubMed:7744004). Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration (PubMed:21317288). Phosphorylates JUN and plays an important role in EGF-induced cell proliferation (PubMed:21177766). Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP (PubMed:21724829). Phosphorylates CASP7, thereby preventing its activity (PubMed:21555521, PubMed:27889207). Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis (PubMed:19273597, PubMed:19923322). On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway (PubMed:9171063, PubMed:12853446, PubMed:16617111). Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (PubMed:15234964). {ECO:0000269|PubMed:12853446, ECO:0000269|PubMed:15234964, ECO:0000269|PubMed:16617111, ECO:0000269|PubMed:19273597, ECO:0000269|PubMed:19923322, ECO:0000269|PubMed:21177766, ECO:0000269|PubMed:21317288, ECO:0000269|PubMed:21555521, ECO:0000269|PubMed:21724829, ECO:0000269|PubMed:27889207, ECO:0000269|PubMed:33693784, ECO:0000269|PubMed:7744004, ECO:0000269|PubMed:9171063}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.

check button - Retained domain in the 5'-partner of fusion protein (protein functional feature from UniProt).
PartnerHgeneeneHbpTgeneeneTbpENSTBPexonTotalExonProtein feature lociBPlociTotalLenFeatureNote


check button - Retained domain in the 3'-partner of fusion protein (protein functional feature from UniProt).
PartnerHgeneeneHbpTgeneeneTbpENSTBPexonTotalExonProtein feature lociBPlociTotalLenFeatureNote


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Kinase-Substrate Information of PAK2_ANKUB1


check button Phosphorylation target of the kinase
(phosphosite, 03-17-2024)
KinaseKinase UniProt AccKinase speciesSubstrateSubstrate UniProt AccSubstrate phosphorylated residuesSubstrate phosphorylated sites (+/-7AA)Domain
PAK2Q13177humanMYCP01106S388RRNELkRsFFALRDQHLH
PAK2Q13177humanARHGAP15Q53QZ3S292kVCERENsTVPWFVk
PAK2Q13177humanH4C1P62805S47RGGVKrIsGLIyEEtCENP-T_C
PAK2Q13177humanJUNP05412T2______MtAKMETtF
PAK2Q13177humanCALD1Q05682S744GLKVGVSsRINEWLTCaldesmon
PAK2Q13177humanJUNP05412T286RLEEKVKtLKAQNSEbZIP_1
PAK2Q13177humanMYCP01106T373EENVkRRtHNVLERQHLH
PAK2Q13177humanMICAL1Q8TDZ2S817sPERQRLssLNLtPD
PAK2Q13177humanSORT1Q99523S793RFLVHRYsVLQQHAE
PAK2Q13177humanCASP7P55210S239WRsPGRGsWFVQALCPeptidase_C14
PAK2Q13177humanABL1P00519S619PtPPKRsssFREMDG
PAK2Q13177humanPAK2Q13177S192PRPDHTksIytRsVI
PAK2Q13177humanPAK2Q13177S141tVkQKyLsFtPPEkd
PAK2Q13177humanSMAD2Q15796S467sVRCssMs_______
PAK2Q13177humanNF2P35240S518DTDMKRLsMEIEKEKERM_C
PAK2Q13177humanSMAD2Q15796S417RMCTIRMsFVkGWGAMH2
PAK2Q13177humanEIF4G1Q04637S895RDIARRRsLGNIkFIMIF4G
PAK2Q13177humanARHGAP15Q53QZ3S4____MQKsTNsDTSV
PAK2Q13177humanARHGAP15Q53QZ3S43DRLsQsksMILTDVG
PAK2Q13177humanPAK2Q13177T402PEQSkRstMVGtPYWPkinase
PAK2Q13177humanJUNP05412T8MtAKMETtFYDDALNJun
PAK2Q13177humanSMAD2Q15796S465sPsVRCssMs_____
PAK2Q13177humanMYCP01106T415VVILKkAtAYILsVQHLH
PAK2Q13177humanPXNP49023S272ELDELMAsLsDFkIQ
PAK2Q13177humanCASP7P55210S30DAKPDRssFVPsLFs
PAK2Q13177humanCALD1Q05682S714EGVRNIksMWEkGNVCaldesmon
PAK2Q13177humanARHGAP15Q53QZ3S41HHDRLsQsksMILTD
PAK2Q13177humanMAPK6Q16659S189ySHKGHLsEGLVTkWPkinase
PAK2Q13177humanPXNP49023S274DELMAsLsDFkIQGL
PAK2Q13177humanPAK2Q13177S197TksIytRsVIDPVPA
PAK2Q13177humanCASP7P55210T173FRGDRCktLLEkPKLPeptidase_C14
PAK2Q13177humanCTNNB1P35222S552QDtQRRtsMGGtQQQ
PAK2Q13177humanSMAD2Q15796S464GsPsVRCssMs____
PAK2Q13177humanJUNP05412T89QSSNGHItTtPtPtQJun
PAK2Q13177humanPREX2Q70Z35S1107DTISNRDsYSDCNSN
PAK2Q13177humanRAF1P04049S338RPRGQRDssyyWEIE
PAK2Q13177humanMICAL1Q8TDZ2S960ELALRRQssSPEQQKbMERB_dom
PAK2Q13177humanHACE1Q8IYU2S385LMKNKRDsTEITsIL
PAK2Q13177humanSLC25A5P05141T107LGGVDkRtQFWLyFA
PAK2Q13177humanJUNP05412T93GHItTtPtPtQFLCPJun
PAK2Q13177humanABL1P00519S618APtPPKRsssFREMD


check button Biological Network Integration of This Kinase and Substrates
(GeneMANIA website)

check button Enriched GO biological processes of the phosphorylation target genes of the kinase
KinaseGOIDGO termP.adjust
PAK2IDDescription0.00e+00
PAK2GO:0051017actin filament bundle assembly9.20e-06
PAK2GO:0061572actin filament bundle organization9.20e-06
PAK2GO:0051492regulation of stress fiber assembly7.93e-04
PAK2GO:0110020regulation of actomyosin structure organization7.93e-04
PAK2GO:0031400negative regulation of protein modification process7.93e-04
PAK2GO:0032231regulation of actin filament bundle assembly7.93e-04
PAK2GO:0030038contractile actin filament bundle assembly7.93e-04
PAK2GO:0043149stress fiber assembly7.93e-04
PAK2GO:0007015actin filament organization8.86e-04
PAK2GO:0071560cellular response to transforming growth factor beta stimulus1.30e-03
PAK2GO:0071559response to transforming growth factor beta1.31e-03
PAK2GO:0001933negative regulation of protein phosphorylation1.43e-03
PAK2GO:0048538thymus development1.75e-03
PAK2GO:0042326negative regulation of phosphorylation1.75e-03
PAK2GO:0051496positive regulation of stress fiber assembly1.76e-03
PAK2GO:0032233positive regulation of actin filament bundle assembly2.69e-03
PAK2GO:0045936negative regulation of phosphate metabolic process2.69e-03
PAK2GO:0010563negative regulation of phosphorus metabolic process2.69e-03
PAK2GO:0007440foregut morphogenesis3.13e-03
PAK2GO:2001233regulation of apoptotic signaling pathway3.13e-03
PAK2GO:0031032actomyosin structure organization3.71e-03
PAK2GO:0009950dorsal/ventral axis specification4.07e-03
PAK2GO:0051346negative regulation of hydrolase activity4.07e-03
PAK2GO:0034333adherens junction assembly4.44e-03
PAK2GO:0016358dendrite development4.84e-03
PAK2GO:0048145regulation of fibroblast proliferation5.40e-03
PAK2GO:0007264small GTPase mediated signal transduction5.67e-03
PAK2GO:0045638negative regulation of myeloid cell differentiation5.67e-03
PAK2GO:0060192negative regulation of lipase activity5.67e-03
PAK2GO:0001657ureteric bud development5.67e-03
PAK2GO:0048534hematopoietic or lymphoid organ development5.67e-03
PAK2GO:0051098regulation of binding5.67e-03
PAK2GO:0072163mesonephric epithelium development5.67e-03
PAK2GO:0072164mesonephric tubule development5.67e-03
PAK2GO:0001823mesonephros development6.19e-03
PAK2GO:0110053regulation of actin filament organization6.19e-03
PAK2GO:0048144fibroblast proliferation6.56e-03
PAK2GO:0051056regulation of small GTPase mediated signal transduction7.89e-03
PAK2GO:0007398ectoderm development8.04e-03
PAK2GO:0002065columnar/cuboidal epithelial cell differentiation9.15e-03
PAK2GO:0002053positive regulation of mesenchymal cell proliferation9.35e-03
PAK2GO:0035994response to muscle stretch9.35e-03
PAK2GO:0048011neurotrophin TRK receptor signaling pathway9.35e-03
PAK2GO:0001704formation of primary germ layer9.35e-03
PAK2GO:0044346fibroblast apoptotic process9.78e-03
PAK2GO:0032956regulation of actin cytoskeleton organization1.08e-02
PAK2GO:0031589cell-substrate adhesion1.30e-02
PAK2GO:0033688regulation of osteoblast proliferation1.31e-02
PAK2GO:0072073kidney epithelium development1.34e-02

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Related Drugs to PAK2_ANKUB1


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

check button Distribution of the number of studies mentioning PAK2-ANKUB1 and kinase inhibitors the PubMed Abstract (04-01-2024)

Fusion gene - drug pair 1Fusion gene - drug pair 2PMIDPublication dateDOIStudy title

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Related Diseases to PAK2_ANKUB1


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Related diseases from the literature mentioned this fusion gene and drug.
(PubMed, 04-01-2024)
MeSH IDMeSH term

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource


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Clinical Trials of the Found Drugs/Small Molecules


check button Statistics of the Clinical Trials of the Found Kinase Inibitors from clinicaltrials.gov (06-17-2024)

check button Clinical Trials from clinicaltrials.gov (06-17-2024)

Fusion GeneKinase InhibitorNCT IDStudy StatusPhasesDisease# EnrolmentDate