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Kinase Fusion Gene:SCAF8_LATS1 |
Kinase Fusion Protein Summary |
Kinase Fusion gene summary |
Kinase Fusion partner gene information | Kinase Fusion gene name: SCAF8_LATS1 | KinaseFusionDB ID: KFG5681 | FusionGDB2.0 ID: KFG5681 | Hgene | Tgene | Gene symbol | SCAF8 | LATS1 | Gene ID | 22828 | 9113 | |
Gene name | SR-related CTD associated factor 8 | large tumor suppressor kinase 1 | ||||||||||
Synonyms | RBM16 | WARTS|wts | ||||||||||
Cytomap | 6q25.2 | 6q25.1 | ||||||||||
Type of gene | protein-coding | protein-coding | ||||||||||
Description | SR-related and CTD-associated factor 8CDC5L complex-associated protein 7RNA-binding motif protein 16SR-like CTD-associated factor 8protein SCAF8 | serine/threonine-protein kinase LATS1LATS (large tumor suppressor, Drosophila) homolog 1LATS, large tumor suppressor, homolog 1WARTS protein kinaseh-wartslarge tumor suppressor homolog 1 | ||||||||||
Modification date | 20240411 | 20240407 | ||||||||||
UniProtAcc | Q9UPN6 | O95835 | ||||||||||
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000367178, ENST00000367186, ENST00000417268, ENST00000461219, | ENST00000253339, ENST00000392273, ENST00000542747, ENST00000543571, | |||||||||
Context (manual curation of fusion genes in KinaseFusionDB) | PubMed: SCAF8 [Title/Abstract] AND LATS1 [Title/Abstract] AND fusion [Title/Abstract] | |||||||||||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | SCAF8(155085491)-LATS1(150002102), # samples:1 |
Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
Partner | Gene | GO ID | GO term | PubMed ID |
Hgene | SCAF8 | GO:0032786 | positive regulation of DNA-templated transcription, elongation | 31104839 |
Hgene | SCAF8 | GO:2000805 | negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled | 31104839 |
Tgene | LATS1 | GO:0000086 | G2/M transition of mitotic cell cycle | 15122335 |
Tgene | LATS1 | GO:0000819 | sister chromatid segregation | 15122335 |
Tgene | LATS1 | GO:0006468 | protein phosphorylation | 10518011 |
Tgene | LATS1 | GO:0030833 | regulation of actin filament polymerization | 15220930 |
Tgene | LATS1 | GO:0033138 | positive regulation of peptidyl-serine phosphorylation | 20412773 |
Tgene | LATS1 | GO:0035329 | hippo signaling | 20412773 |
Tgene | LATS1 | GO:0045736 | negative regulation of cyclin-dependent protein serine/threonine kinase activity | 9988268 |
Kinase Fusion gene breakpoints across SCAF8 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
Kinase Fusion gene breakpoints across LATS1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Kinase Fusion Gene Sample Information |
Kinase Fusion gene information. |
Kinase Fusion gene information from four resources (ChiTars 5.0, ChimerDB 4.0, COSMIC, and CCLE) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Sample | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp |
ChiTaRS5.0 | BE006984 | SCAF8 | chr6 | 155085491 | LATS1 | chr6 | 150002102 |
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Kinase Fusion ORF Analysis |
Kinase Fusion information from ORFfinder translation from full-length transcript sequence from KinaseFusionDB. |
Henst | Tenst | Hgene | Hchr | Hbp | Tgene | Tchr | Tbp | Seq length (transcript) | Seq length (amino acids) |
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Kinase Fusion Amino Acid Sequences |
For individual full-length fusion transcript sequence from KinaseFusionDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
>Henst_Tenst_Hgene_Hchr_Hbp_Tgene_Tchr_Tbp_length(fusion AA)_AAseq |
Multiple Sequence Alignment of All Fusion Protein Isoforms |
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Kinase Fusion Protein Functional Features |
Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/:155085491/:150002102) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
Main function of each fusion partner protein. (from UniProt) |
Hgene | Tgene |
SCAF8 | LATS1 |
FUNCTION: Anti-terminator protein required to prevent early mRNA termination during transcription (PubMed:31104839). Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins (PubMed:31104839). Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation (PubMed:31104839). Independently of SCAF4, also acts as a positive regulator of transcript elongation (PubMed:31104839). {ECO:0000269|PubMed:31104839}. | FUNCTION: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668). {ECO:0000269|PubMed:10518011, ECO:0000269|PubMed:10831611, ECO:0000269|PubMed:15122335, ECO:0000269|PubMed:15220930, ECO:0000269|PubMed:18158288, ECO:0000269|PubMed:19927127, ECO:0000269|PubMed:28068668}. |
Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
- Retained domain in the 5'-partner of fusion protein (protein functional feature from UniProt). |
Partner | Hgeneene | Hbp | Tgeneene | Tbp | ENST | BPexon | TotalExon | Protein feature loci | BPloci | TotalLen | Feature | Note |
- Retained domain in the 3'-partner of fusion protein (protein functional feature from UniProt). |
Partner | Hgeneene | Hbp | Tgeneene | Tbp | ENST | BPexon | TotalExon | Protein feature loci | BPloci | TotalLen | Feature | Note |
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Kinase-Substrate Information of SCAF8_LATS1 |
Phosphorylation target of the kinase (phosphosite, 03-17-2024) |
Kinase | Kinase UniProt Acc | Kinase species | Substrate | Substrate UniProt Acc | Substrate phosphorylated residues | Substrate phosphorylated sites (+/-7AA) | Domain |
LATS1 | O95835 | human | INCENP | Q9NQS7 | S894 | RYHKRtssAVWNsPP | |
LATS1 | O95835 | human | YAP1 | P46937 | S397 | tyHSRDEstDsGLsM | |
LATS1 | O95835 | human | YAP1 | P46937 | S61 | IVHVRGDsEtDLEAL | |
LATS1 | O95835 | human | MAP3K11 | Q16584 | T477 | HVRRRRGtFKRSKLR | |
LATS1 | O95835 | human | SASH1 | O94885 | S407 | LSHGRtCsFGGFDLT | SLY |
LATS1 | O95835 | human | CDC26 | Q8NHZ8 | T7 | _MLRRKPtRLELKLD | ANAPC_CDC26 |
LATS1 | O95835 | human | RPTOR | Q8N122 | S606 | SAHEkLYsLLSDPIP | HEAT |
LATS1 | O95835 | human | SNAI2 | O43623 | T208 | QGHIRtHtGEkPFSC | |
LATS1 | O95835 | human | LATS1 | O95835 | S674 | EMMRVGLsQDAQDQM | |
LATS1 | O95835 | human | LATS1 | O95835 | S909 | HQRCLAHsLVGTPNY | Pkinase |
LATS1 | O95835 | human | YAP1 | P46937 | S381 | LRTMTTNssDPFLNs | |
LATS1 | O95835 | human | YAP1 | P46937-2 | S61 | IVHVRGDsEtDLEAL | |
LATS1 | O95835 | human | MTF1 | Q14872 | S152 | EGCPRTysTAGNLRT | zf-C2H2 |
LATS1 | O95835 | human | PRPS1 | P60891 | S285 | EDkMKHCsKIQVIDI | Pribosyl_synth |
LATS1 | O95835 | human | RAF1 | P04049 | S259 | sQRQRststPNVHMV | |
LATS1 | O95835 | human | KIF23 | Q02241 | S814 | LRHRRsRsAGDRWVD | MKLP1_Arf_bdg |
LATS1 | O95835 | human | AMOT | Q4VCS5 | S175 | QGHVRSLsERLMQMS | |
LATS1 | O95835 | human | YAP1 | P46937-2 | S109 | KsHsRQAstDAGtAG | |
LATS1 | O95835 | human | KIF23 | Q02241 | S716 | QLHRRsNsCsSISVA | |
LATS1 | O95835 | human | YAP1 | P46937 | S127 | PQHVRAHssPAsLQL | |
LATS1 | O95835 | human | LATS1 | O95835 | S1049 | VDPDkLWsDDNEEEN | |
LATS1 | O95835 | human | PRPS2 | P11908 | T285 | EDkMkHCtKIQVIDI | Pribosyl_synth |
LATS1 | O95835 | human | YAP1 | P46937-2 | S127 | PQHVRAHssPASLQL | |
LATS1 | O95835 | human | YAP1 | P46937 | S109 | ksHsRQAstDAGtAG | |
LATS1 | O95835 | human | YAP1 | P46937-2 | S164 | AQHLRQssFEIPDDV | |
LATS1 | O95835 | human | YAP1 | P46937-2 | S381 | TYHSRDEsTDsGLsM | |
LATS1 | O95835 | human | WWTR1 | Q9GZV5 | S89 | AQHVRsHssPAsLQL |
Biological Network Integration of This Kinase and Substrates (GeneMANIA website) |
Enriched GO biological processes of the phosphorylation target genes of the kinase |
Kinase | GOID | GO term | P.adjust |
LATS1 | ID | Description | 0.00e+00 |
LATS1 | GO:0035329 | hippo signaling | 2.51e-05 |
LATS1 | GO:0051256 | mitotic spindle midzone assembly | 4.29e-03 |
LATS1 | GO:0000022 | mitotic spindle elongation | 4.29e-03 |
LATS1 | GO:0072182 | regulation of nephron tubule epithelial cell differentiation | 4.29e-03 |
LATS1 | GO:0060828 | regulation of canonical Wnt signaling pathway | 4.29e-03 |
LATS1 | GO:0051231 | spindle elongation | 4.29e-03 |
LATS1 | GO:0051255 | spindle midzone assembly | 4.29e-03 |
LATS1 | GO:0072160 | nephron tubule epithelial cell differentiation | 4.29e-03 |
LATS1 | GO:2001237 | negative regulation of extrinsic apoptotic signaling pathway | 4.29e-03 |
LATS1 | GO:0071900 | regulation of protein serine/threonine kinase activity | 4.29e-03 |
LATS1 | GO:2000696 | regulation of epithelial cell differentiation involved in kidney development | 4.29e-03 |
LATS1 | GO:0033138 | positive regulation of peptidyl-serine phosphorylation | 4.29e-03 |
LATS1 | GO:0060070 | canonical Wnt signaling pathway | 5.11e-03 |
LATS1 | GO:0006098 | pentose-phosphate shunt | 5.52e-03 |
LATS1 | GO:0030111 | regulation of Wnt signaling pathway | 5.52e-03 |
LATS1 | GO:0072234 | metanephric nephron tubule development | 5.52e-03 |
LATS1 | GO:0006740 | NADPH regeneration | 5.52e-03 |
LATS1 | GO:0072202 | cell differentiation involved in metanephros development | 5.52e-03 |
LATS1 | GO:0033135 | regulation of peptidyl-serine phosphorylation | 5.52e-03 |
LATS1 | GO:0071902 | positive regulation of protein serine/threonine kinase activity | 5.52e-03 |
LATS1 | GO:0090090 | negative regulation of canonical Wnt signaling pathway | 5.52e-03 |
LATS1 | GO:0072170 | metanephric tubule development | 5.52e-03 |
LATS1 | GO:0072243 | metanephric nephron epithelium development | 5.52e-03 |
LATS1 | GO:1900180 | regulation of protein localization to nucleus | 5.52e-03 |
LATS1 | GO:0045598 | regulation of fat cell differentiation | 5.63e-03 |
LATS1 | GO:0045667 | regulation of osteoblast differentiation | 5.86e-03 |
LATS1 | GO:0051156 | glucose 6-phosphate metabolic process | 6.13e-03 |
LATS1 | GO:0072207 | metanephric epithelium development | 6.13e-03 |
LATS1 | GO:2001236 | regulation of extrinsic apoptotic signaling pathway | 6.13e-03 |
LATS1 | GO:0043507 | positive regulation of JUN kinase activity | 6.59e-03 |
LATS1 | GO:0030856 | regulation of epithelial cell differentiation | 7.23e-03 |
LATS1 | GO:0030178 | negative regulation of Wnt signaling pathway | 7.25e-03 |
LATS1 | GO:0009156 | ribonucleoside monophosphate biosynthetic process | 7.42e-03 |
LATS1 | GO:0033146 | regulation of intracellular estrogen receptor signaling pathway | 7.42e-03 |
LATS1 | GO:0016055 | Wnt signaling pathway | 7.86e-03 |
LATS1 | GO:0198738 | cell-cell signaling by wnt | 7.86e-03 |
LATS1 | GO:0050673 | epithelial cell proliferation | 8.91e-03 |
LATS1 | GO:0009124 | nucleoside monophosphate biosynthetic process | 9.99e-03 |
LATS1 | GO:0006739 | NADP metabolic process | 9.99e-03 |
LATS1 | GO:0043506 | regulation of JUN kinase activity | 9.99e-03 |
LATS1 | GO:0001825 | blastocyst formation | 1.02e-02 |
LATS1 | GO:0010837 | regulation of keratinocyte proliferation | 1.04e-02 |
LATS1 | GO:0032467 | positive regulation of cytokinesis | 1.04e-02 |
LATS1 | GO:0035850 | epithelial cell differentiation involved in kidney development | 1.04e-02 |
LATS1 | GO:0000819 | sister chromatid segregation | 1.11e-02 |
LATS1 | GO:0097191 | extrinsic apoptotic signaling pathway | 1.14e-02 |
LATS1 | GO:0030520 | intracellular estrogen receptor signaling pathway | 1.28e-02 |
LATS1 | GO:2001234 | negative regulation of apoptotic signaling pathway | 1.30e-02 |
LATS1 | GO:0045444 | fat cell differentiation | 1.34e-02 |
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Related Drugs to SCAF8_LATS1 |
Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
Distribution of the number of studies mentioning SCAF8-LATS1 and kinase inhibitors the PubMed Abstract (04-01-2024) |
Fusion gene - drug pair 1 | Fusion gene - drug pair 2 | PMID | Publication date | DOI | Study title |
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Related Diseases to SCAF8_LATS1 |
Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
Related diseases from the literature mentioned this fusion gene and drug. (PubMed, 04-01-2024) |
MeSH ID | MeSH term |
Diseases associated with fusion partners. (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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Clinical Trials of the Found Drugs/Small Molecules |
Statistics of the Clinical Trials of the Found Kinase Inibitors from clinicaltrials.gov (06-17-2024) |
Clinical Trials from clinicaltrials.gov (06-17-2024) |
Fusion Gene | Kinase Inhibitor | NCT ID | Study Status | Phases | Disease | # Enrolment | Date |