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Fusion Protein:ZNF586-AURKC

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ZNF586-AURKC
	FusionPDB ID: 102456
	FusionGDB2.0 ID: 102456
		Hgene	Tgene
	Gene symbol	ZNF586	AURKC
	Gene ID	54807	6795
	Gene name	zinc finger protein 586	aurora kinase C
	Synonyms	-	AIE2\|AIK3\|ARK3\|AurC\|HEL-S-90\|SPGF5\|STK13\|aurora-C
	Cytomap	19q13.43	19q13.43
	Type of gene	protein-coding	protein-coding
	Description	zinc finger protein 586	aurora kinase CARK-3aurora 3aurora-related kinase 3aurora/IPL1-related kinase 3aurora/IPL1/EG2 protein 2epididymis secretory protein Li 90serine/threonine kinase 13 (aurora/IPL1-like)serine/threonine-protein kinase 13serine/threonine-protein kina
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000598183, ENST00000598885, ENST00000391702, ENST00000396150, ENST00000396154, ENST00000599802,	ENST00000302804, ENST00000448930, ENST00000598785, ENST00000599062, ENST00000415300,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 5 X 5=150	5 X 4 X 3=60
	# samples	7	5
	** MAII score	log2(7/150*10)=-1.09953567355091 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(5/60*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: ZNF586 [Title/Abstract] AND AURKC [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: ZNF586 [Title/Abstract] AND AURKC [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ZNF586(58301768)-AURKC(57743110), # samples:1
Anticipated loss of major functional domain due to fusion event.	ZNF586-AURKC seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ZNF586-AURKC seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ZNF586-AURKC seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ZNF586-AURKC seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	AURKC	GO:0006468	protein phosphorylation	15670791

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:58301768/chr19:57743110)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across ZNF586 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across AURKC (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000598885

ZNF586

chr19

58301768

ENST00000415300

AURKC

chr19

57743110

1217

345

1216

400

ENST00000598183

ZNF586

chr19

58301768

ENST00000415300

AURKC

chr19

57743110

1211

339

1210

400

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000598885	ENST00000415300	ZNF586	chr19	58301768	+	AURKC	chr19	57743110	+	0.003699466	0.99630046
ENST00000598183	ENST00000415300	ZNF586	chr19	58301768	+	AURKC	chr19	57743110	+	0.003528949	0.99647105

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for ZNF586-AURKC

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
ZNF586	chr19	58301768	AURKC	chr19	57743110	339	66	RVMAAAAALRAPAQSSVTFEDVAVNF
ZNF586	chr19	58301768	AURKC	chr19	57743110	345	66	RVMAAAAALRAPAQSSVTFEDVAVNF

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Potential FusionNeoAntigen Information of ZNF586-AURKC in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

ZNF586-AURKC_58301768_57743110.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B14:01	LRAPAQSSV	0.9979	0.8804	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:24	LRAPAQSSV	0.9979	0.6364	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B14:02	LRAPAQSSV	0.9979	0.8804	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:06	LRAPAQSSV	0.9978	0.884	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:10	APAQSSVTF	0.9976	0.5127	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:01	APAQSSVTF	0.9969	0.8452	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:01	LRAPAQSSV	0.9967	0.8254	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:08	APAQSSVTF	0.9938	0.7326	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:03	APAQSSVTF	0.9797	0.8095	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:02	APAQSSVTF	0.9776	0.9465	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:05	APAQSSVTF	0.975	0.5762	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:18	APAQSSVTF	0.8487	0.8149	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:02	APAQSSVTF	0.7605	0.9543	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:04	APAQSSVTF	0.7605	0.9543	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:10	APAQSSVTF	0.699	0.558	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:10	LRAPAQSSV	0.3602	0.54	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B82:01	APAQSSVTF	0.1197	0.5728	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B81:01	APAQSSVTF	0.0878	0.5499	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B58:02	RAPAQSSVTF	0.9967	0.9239	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:17	RAPAQSSVTF	0.9941	0.8501	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:16	RAPAQSSVTF	0.994	0.7224	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B58:01	RAPAQSSVTF	0.9864	0.9021	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:02	RAPAQSSVTF	0.9859	0.5808	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-A02:13	ALRAPAQSSV	0.9762	0.68	7	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B57:03	RAPAQSSVTF	0.9696	0.9609	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:08	RAPAQSSVTF	0.9435	0.7424	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-A02:38	ALRAPAQSSV	0.9372	0.705	7	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-A02:27	ALRAPAQSSV	0.9332	0.5346	7	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:01	RAPAQSSVTF	0.9024	0.7687	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:03	RAPAQSSVTF	0.8285	0.7391	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B13:02	AQSSVTFEDV	0.7491	0.9212	12	22
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B27:04	LRAPAQSSVTF	1	0.505	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:18	LRAPAQSSVTF	0.9987	0.5822	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:08	LRAPAQSSVTF	0.9942	0.6887	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:03	LRAPAQSSVTF	0.9922	0.6506	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:01	LRAPAQSSVTF	0.9915	0.6918	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:02	LRAPAQSSVTF	0.979	0.8374	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:04	LRAPAQSSVTF	0.979	0.8374	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:17	RAPAQSSV	0.9861	0.9619	9	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:30	RAPAQSSV	0.8846	0.9605	9	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:09	LRAPAQSSV	0.9969	0.7591	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:31	APAQSSVTF	0.9965	0.8413	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:12	LRAPAQSSV	0.9962	0.825	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B73:01	LRAPAQSSV	0.9938	0.7951	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:05	LRAPAQSSV	0.991	0.8188	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:05	LRAPAQSSV	0.9888	0.919	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:21	APAQSSVTF	0.9797	0.9222	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:27	LRAPAQSSV	0.9683	0.9149	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:95	LRAPAQSSV	0.9662	0.5415	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:13	LRAPAQSSV	0.9469	0.9342	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:29	LRAPAQSSV	0.9078	0.8731	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:12	APAQSSVTF	0.8898	0.617	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:46	LRAPAQSSV	0.8603	0.8732	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:10	LRAPAQSSV	0.8164	0.9378	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:80	LRAPAQSSV	0.8159	0.9466	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:67	LRAPAQSSV	0.8159	0.9466	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:19	LRAPAQSSV	0.8014	0.7689	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:12	APAQSSVTF	0.7605	0.9543	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B14:03	LRAPAQSSV	0.7219	0.8425	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B14:03	APAQSSVTF	0.6972	0.8613	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:10	APAQSSVTF	0.4926	0.9361	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B51:07	APAQSSVTF	0.4574	0.7953	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B42:02	APAQSSVTF	0.3983	0.5718	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:08	APAQSSVTF	0.3137	0.8861	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B42:01	APAQSSVTF	0.2585	0.5589	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C08:03	APAQSSVTF	0.2112	0.9891	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C04:14	APAQSSVTF	0.1021	0.9508	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C12:16	LRAPAQSSV	0.0557	0.9557	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C04:07	APAQSSVTF	0.0083	0.9237	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C04:10	APAQSSVTF	0.0078	0.9256	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:12	RAPAQSSVTF	0.9892	0.75	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:17	RAPAQSSVTF	0.9804	0.9633	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:30	RAPAQSSVTF	0.9477	0.9596	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:04	RAPAQSSVTF	0.937	0.5575	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:12	APAQSSVTFE	0.9183	0.6399	10	20
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:10	RAPAQSSVTF	0.693	0.8791	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:95	LRAPAQSSVTF	0.9993	0.6346	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:80	LRAPAQSSVTF	0.9988	0.9531	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:19	LRAPAQSSVTF	0.9988	0.7907	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:67	LRAPAQSSVTF	0.9988	0.9531	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:46	LRAPAQSSVTF	0.9977	0.8975	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:10	LRAPAQSSVTF	0.9974	0.9538	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:10	LRAPAQSSVTF	0.9952	0.893	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:12	LRAPAQSSVTF	0.979	0.8374	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:12	LRAPAQSSVTF	0.9781	0.6763	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:03	RAPAQSSV	0.997	0.9643	9	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:02	RAPAQSSV	0.9948	0.9612	9	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:13	APAQSSVTF	0.998	0.8778	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:77	APAQSSVTF	0.9969	0.8452	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:23	APAQSSVTF	0.9969	0.8437	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:31	LRAPAQSSV	0.9968	0.8239	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:20	APAQSSVTF	0.9962	0.8887	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C06:08	LRAPAQSSV	0.9954	0.9841	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:24	APAQSSVTF	0.9804	0.8588	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:17	APAQSSVTF	0.9764	0.7634	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:30	APAQSSVTF	0.9764	0.7634	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:11	APAQSSVTF	0.9757	0.9136	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:13	APAQSSVTF	0.974	0.8161	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:01	LRAPAQSSV	0.9694	0.526	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:09	LRAPAQSSV	0.9356	0.6583	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:13	APAQSSVTF	0.8677	0.8761	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:04	LRAPAQSSV	0.8513	0.9196	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:02	LRAPAQSSV	0.8159	0.9466	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C06:06	LRAPAQSSV	0.7911	0.9928	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:09	APAQSSVTF	0.7605	0.9543	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:22	LRAPAQSSV	0.7571	0.6511	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B67:01	APAQSSVTF	0.5072	0.7984	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B18:04	APAQSSVTF	0.5067	0.8676	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:02	APAQSSVTF	0.4713	0.9486	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B78:02	APAQSSVTF	0.4473	0.6244	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B39:11	APAQSSVTF	0.3634	0.8601	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:43	APAQSSVTF	0.3176	0.8472	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:08	APAQSSVTF	0.3176	0.8402	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C06:02	LRAPAQSSV	0.3128	0.9924	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C06:17	LRAPAQSSV	0.3128	0.9924	8	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:11	APAQSSVTF	0.2962	0.8512	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B18:07	APAQSSVTF	0.2604	0.8089	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C08:01	APAQSSVTF	0.2112	0.9891	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B18:08	APAQSSVTF	0.1671	0.8845	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B82:02	APAQSSVTF	0.1197	0.5728	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C04:04	APAQSSVTF	0.0936	0.9647	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C04:01	APAQSSVTF	0.0083	0.9237	10	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:11	RAPAQSSVTF	0.9987	0.7361	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:43	RAPAQSSVTF	0.9985	0.7214	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:08	RAPAQSSVTF	0.9985	0.7172	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B58:06	RAPAQSSVTF	0.998	0.8829	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-A02:03	ALRAPAQSSV	0.9968	0.5728	7	17
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B57:04	RAPAQSSVTF	0.9955	0.7662	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:11	RAPAQSSVTF	0.994	0.7935	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:09	RAPAQSSVTF	0.9875	0.6112	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:22	RAPAQSSVTF	0.9859	0.5808	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:03	RAPAQSSVTF	0.9854	0.9634	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C01:02	RAPAQSSVTF	0.9812	0.962	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B57:02	RAPAQSSVTF	0.9796	0.8758	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:30	RAPAQSSVTF	0.9621	0.6869	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:17	RAPAQSSVTF	0.9621	0.6869	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:77	RAPAQSSVTF	0.9024	0.7687	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:23	RAPAQSSVTF	0.8964	0.7484	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:13	RAPAQSSVTF	0.8388	0.7471	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B67:01	RAPAQSSVTF	0.7252	0.7624	9	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B27:06	LRAPAQSSVTF	1	0.5304	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:01	LRAPAQSSVTF	0.9993	0.6314	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:22	LRAPAQSSVTF	0.9991	0.7057	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:11	LRAPAQSSVTF	0.999	0.697	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:43	LRAPAQSSVTF	0.9989	0.6954	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:08	LRAPAQSSVTF	0.9988	0.6864	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-C07:02	LRAPAQSSVTF	0.9988	0.9531	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:11	LRAPAQSSVTF	0.9981	0.7376	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B15:68	LRAPAQSSVTF	0.9979	0.5165	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:17	LRAPAQSSVTF	0.9961	0.5953	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:30	LRAPAQSSVTF	0.9961	0.5953	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B67:01	LRAPAQSSVTF	0.9924	0.797	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:23	LRAPAQSSVTF	0.9923	0.7029	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:77	LRAPAQSSVTF	0.9915	0.6918	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B07:09	LRAPAQSSVTF	0.9902	0.5403	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:09	LRAPAQSSVTF	0.979	0.8374	8	19
ZNF586-AURKC	chr19	58301768	chr19	57743110	339	HLA-B35:24	LRAPAQSSVTF	0.9724	0.735	8	19

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Potential FusionNeoAntigen Information of ZNF586-AURKC in HLA II

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene

Hchr

Hbp

Tgene

Tchr

Tbp

HLA II

FusionNeoAntigen peptide

Neoantigen start (at BP 13)

Neoantigen end (at BP 13)

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Fusion breakpoint peptide structures of ZNF586-AURKC

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
51	AALRAPAQSSVTFE	ZNF586	AURKC	chr19	58301768	chr19	57743110	339

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of ZNF586-AURKC

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	51	AALRAPAQSSVTFE	-4.93023	-5.69623
HLA-B14:02	3BVN	51	AALRAPAQSSVTFE	-4.03623	-4.22633
HLA-B52:01	3W39	51	AALRAPAQSSVTFE	-6.76045	-6.95055
HLA-B52:01	3W39	51	AALRAPAQSSVTFE	-4.82304	-5.58904
HLA-A11:01	4UQ2	51	AALRAPAQSSVTFE	-6.12244	-6.88844
HLA-A24:02	5HGA	51	AALRAPAQSSVTFE	-6.83882	-7.60482
HLA-A24:02	5HGA	51	AALRAPAQSSVTFE	-5.32749	-5.51759
HLA-B44:05	3DX8	51	AALRAPAQSSVTFE	-7.07991	-7.84591
HLA-B44:05	3DX8	51	AALRAPAQSSVTFE	-6.27909	-6.46919
HLA-A02:01	6TDR	51	AALRAPAQSSVTFE	-3.83325	-4.59925

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Vaccine Design for the FusionNeoAntigens of ZNF586-AURKC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
ZNF586-AURKC	chr19	58301768	chr19	57743110	10	19	APAQSSVTF	CCTCCCTGGTGGCTACAGCAAACCAAA
ZNF586-AURKC	chr19	58301768	chr19	57743110	10	20	APAQSSVTFE	CCTCCCTGGTGGCTACAGCAAACCAAACAG
ZNF586-AURKC	chr19	58301768	chr19	57743110	12	22	AQSSVTFEDV	TGGTGGCTACAGCAAACCAAACAGCCCAGC
ZNF586-AURKC	chr19	58301768	chr19	57743110	7	17	ALRAPAQSSV	CACTTATATCCTCCCTGGTGGCTACAGCAA
ZNF586-AURKC	chr19	58301768	chr19	57743110	8	17	LRAPAQSSV	TTATATCCTCCCTGGTGGCTACAGCAA
ZNF586-AURKC	chr19	58301768	chr19	57743110	8	19	LRAPAQSSVTF	TTATATCCTCCCTGGTGGCTACAGCAAACCAAA
ZNF586-AURKC	chr19	58301768	chr19	57743110	9	17	RAPAQSSV	TATCCTCCCTGGTGGCTACAGCAA
ZNF586-AURKC	chr19	58301768	chr19	57743110	9	19	RAPAQSSVTF	TATCCTCCCTGGTGGCTACAGCAAACCAAA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene

Hchr

Hbp

Tchr

Tbp

Start in +/-13AA

End in +/-13AA

FusionNeoAntigen peptide

FusionNEoAntigen RNA sequence

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Information of the samples that have these potential fusion neoantigens of ZNF586-AURKC

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
UCEC	ZNF586-AURKC	chr19	58301768	ENST00000598183	chr19	57743110	ENST00000415300	TCGA-PG-A914

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Potential target of CAR-T therapy development for ZNF586-AURKC

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to ZNF586-AURKC

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to ZNF586-AURKC

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)