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Fusion Protein:AK5-USP33

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: AK5-USP33
	FusionPDB ID: 3250
	FusionGDB2.0 ID: 3250
		Hgene	Tgene
	Gene symbol	AK5	USP33
	Gene ID	26289	23032
	Gene name	adenylate kinase 5	ubiquitin specific peptidase 33
	Synonyms	AK6	VDU1
	Cytomap	1p31.1	1p31.1
	Type of gene	protein-coding	protein-coding
	Description	adenylate kinase isoenzyme 5AK 5ATP-AMP transphosphorylase 5adenylate kinase 6	ubiquitin carboxyl-terminal hydrolase 33VHL-interacting deubiquitinating enzyme 1deubiquitinating enzyme 33pVHL-interacting deubiquitinating enzyme 1ubiquitin thioesterase 33ubiquitin thiolesterase 33ubiquitin-specific-processing protease 33
	Modification date	20200313	20200313
	UniProtAcc	Q9Y6K8 Main function of 5'-partner protein: FUNCTION: Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity. {ECO:0000269\|PubMed:19647735, ECO:0000269\|PubMed:23416111}.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000344720, ENST00000354567, ENST00000478255, ENST00000317704,	ENST00000370792, ENST00000528150, ENST00000357428, ENST00000370793, ENST00000370794,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	5 X 5 X 3=75	13 X 11 X 8=1144
	# samples	5	16
	** MAII score	log2(5/75*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(16/1144*10)=-2.83794324189103 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: AK5 [Title/Abstract] AND USP33 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: AK5 [Title/Abstract] AND USP33 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	AK5(78001723)-USP33(78167156), # samples:1
Anticipated loss of major functional domain due to fusion event.	AK5-USP33 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. AK5-USP33 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. AK5-USP33 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. AK5-USP33 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	AK5	GO:0006165	nucleoside diphosphate phosphorylation	23416111
Hgene	AK5	GO:0009142	nucleoside triphosphate biosynthetic process	23416111
Tgene	USP33	GO:0009267	cellular response to starvation	24056301
Tgene	USP33	GO:0016579	protein deubiquitination	11739384\|19363159\|19424180\|23486064
Tgene	USP33	GO:0070536	protein K63-linked deubiquitination	19363159
Tgene	USP33	GO:0071108	protein K48-linked deubiquitination	19363159

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:78001723/chr1:78167156)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across AK5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across USP33 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000354567	AK5	chr1	78001723	-	ENST00000370794	USP33	chr1	78167156	-	3543	1883	161	2212	683
ENST00000354567	AK5	chr1	78001723	-	ENST00000357428	USP33	chr1	78167156	-	3539	1883	161	2212	683
ENST00000354567	AK5	chr1	78001723	-	ENST00000370793	USP33	chr1	78167156	-	3539	1883	161	2212	683
ENST00000344720	AK5	chr1	78001723	-	ENST00000370794	USP33	chr1	78167156	-	4228	2568	1014	2897	627
ENST00000344720	AK5	chr1	78001723	-	ENST00000357428	USP33	chr1	78167156	-	4224	2568	1014	2897	627
ENST00000344720	AK5	chr1	78001723	-	ENST00000370793	USP33	chr1	78167156	-	4224	2568	1014	2897	627
ENST00000478255	AK5	chr1	78001723	-	ENST00000370794	USP33	chr1	78167156	-	2086	426	249	755	168
ENST00000478255	AK5	chr1	78001723	-	ENST00000357428	USP33	chr1	78167156	-	2082	426	249	755	168
ENST00000478255	AK5	chr1	78001723	-	ENST00000370793	USP33	chr1	78167156	-	2082	426	249	755	168

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000354567	ENST00000370794	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.000941334	0.99905866
ENST00000354567	ENST00000357428	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.000961071	0.99903893
ENST00000354567	ENST00000370793	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.000961071	0.99903893
ENST00000344720	ENST00000370794	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.000322145	0.9996779
ENST00000344720	ENST00000357428	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.000328653	0.99967134
ENST00000344720	ENST00000370793	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.000328653	0.99967134
ENST00000478255	ENST00000370794	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.00220548	0.9977945
ENST00000478255	ENST00000357428	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.002307484	0.9976926
ENST00000478255	ENST00000370793	AK5	chr1	78001723	-	USP33	chr1	78167156	-	0.002307484	0.9976926

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for AK5-USP33

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
AK5	chr1	78001723	USP33	chr1	78167156	1883	574	VIAYYETKTQLHKLNRAFQKEDSPAT
AK5	chr1	78001723	USP33	chr1	78167156	2568	518	VIAYYETKTQLHKLNRAFQKEDSPAT
AK5	chr1	78001723	USP33	chr1	78167156	426	59	VIAYYETKTQLHKLNRAFQKEDSPAT

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Potential FusionNeoAntigen Information of AK5-USP33 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

AK5-USP33_78001723_78167156.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:18	LHKLNRAF	0.9715	0.7734	10	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:01	QLHKLNRAF	0.9937	0.8435	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:25	QLHKLNRAF	0.8817	0.9351	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:02	QLHKLNRAF	0.8802	0.9526	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:03	QLHKLNRAF	0.5138	0.7991	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-A32:13	QLHKLNRAF	0.2606	0.9046	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B13:01	QLHKLNRAF	0.0053	0.9246	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:01	TQLHKLNRAF	0.9982	0.7747	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:03	TQLHKLNRAF	0.9125	0.681	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:07	QLHKLNRAF	0.9851	0.6487	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:04	QLHKLNRAF	0.9526	0.8304	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:21	QLHKLNRAF	0.8804	0.9359	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:31	QLHKLNRAF	0.4453	0.9574	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:05	QLHKLNRAF	0.374	0.9511	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:07	TQLHKLNRAF	0.9904	0.5707	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-C04:10	YYETKTQLHKL	0.9992	0.5767	3	14
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:135	QLHKLNRAF	0.9939	0.8303	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:33	QLHKLNRAF	0.9937	0.8435	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:34	QLHKLNRAF	0.9937	0.8435	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:125	QLHKLNRAF	0.9937	0.8435	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:27	QLHKLNRAF	0.9936	0.8266	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:24	QLHKLNRAF	0.9887	0.8129	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:50	QLHKLNRAF	0.9873	0.9164	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:35	QLHKLNRAF	0.9845	0.7528	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-A30:01	KTQLHKLNR	0.9845	0.701	7	16
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-A25:01	ETKTQLHKL	0.9709	0.8228	5	14
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:12	QLHKLNRAF	0.9609	0.7551	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:53	QLHKLNRAF	0.938	0.8057	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:39	QLHKLNRAF	0.8806	0.8467	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:68	QLHKLNRAF	0.848	0.575	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:54	QLHKLNRAF	0.8432	0.7903	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:13	QLHKLNRAF	0.7295	0.6725	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-A69:01	ETKTQLHKL	0.7159	0.5793	5	14
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B08:12	ETKTQLHKL	0.6557	0.5463	5	14
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:73	QLHKLNRAF	0.6428	0.7933	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-A32:01	QLHKLNRAF	0.6123	0.9521	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:30	QLHKLNRAF	0.4316	0.8081	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:20	QLHKLNRAF	0.3741	0.9674	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B35:28	QLHKLNRAF	0.3172	0.9677	9	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:135	TQLHKLNRAF	0.9982	0.781	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:24	TQLHKLNRAF	0.9982	0.7428	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:33	TQLHKLNRAF	0.9982	0.7747	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:34	TQLHKLNRAF	0.9982	0.7747	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:125	TQLHKLNRAF	0.9982	0.7747	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:27	TQLHKLNRAF	0.9981	0.7641	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:53	TQLHKLNRAF	0.9961	0.7247	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:68	TQLHKLNRAF	0.9957	0.5077	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:50	TQLHKLNRAF	0.9905	0.8594	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:54	TQLHKLNRAF	0.9864	0.7058	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:35	TQLHKLNRAF	0.9857	0.6773	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B40:04	YETKTQLHKL	0.9813	0.5874	4	14
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-B15:12	TQLHKLNRAF	0.977	0.6977	8	18
AK5-USP33	chr1	78001723	chr1	78167156	2568	HLA-C18:01	YYETKTQLHKL	0.9978	0.6348	3	14

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Potential FusionNeoAntigen Information of AK5-USP33 in HLA II

AK5-USP33_78001723_78167156.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0802	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0802	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0804	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0804	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0804	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0804	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0805	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0806	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0806	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0809	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0809	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0810	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0812	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0813	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0815	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0820	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0820	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0820	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0820	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0821	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0821	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0822	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0822	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0824	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0824	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0828	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0828	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0828	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0828	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0830	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0831	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0831	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0831	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-0831	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1101	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1101	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1101	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1102	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1103	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1103	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1104	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1104	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1104	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1104	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1105	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1105	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1106	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1106	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1106	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1106	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1108	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1108	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1109	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1109	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1109	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1110	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1110	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1110	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1111	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1111	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1112	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1112	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1112	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1113	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1113	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1115	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1115	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1115	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1116	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1118	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1118	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1118	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1118	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1119	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1119	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1121	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1124	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1124	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1124	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1125	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1125	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1125	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1125	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1127	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1127	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1128	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1128	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1128	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1129	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1129	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1129	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1131	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1131	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1132	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1132	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1133	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1133	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1133	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1134	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1134	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1135	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1135	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1135	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1135	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1136	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1137	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1137	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1138	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1138	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1138	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1138	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1139	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1139	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1139	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1141	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1141	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1142	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1142	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1142	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1142	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1143	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1143	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1143	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1143	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1144	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1144	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1144	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1144	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1145	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1146	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1146	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1146	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1146	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1147	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1147	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1147	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1147	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1148	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1149	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1149	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1149	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1150	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1150	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1150	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1150	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1151	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1151	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1151	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1154	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1154	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1154	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1154	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1155	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1156	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1156	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1156	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1156	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1157	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1157	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1157	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1157	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1158	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1158	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1158	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1158	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1159	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1159	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1160	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1160	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1160	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1160	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1161	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1161	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1161	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1162	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1162	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1162	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1163	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1163	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1164	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1165	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1166	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1166	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1166	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1167	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1167	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1167	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1167	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1169	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1169	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1172	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1172	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1172	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1173	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1174	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1174	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1174	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1175	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1175	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1175	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1176	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1176	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1177	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1177	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1177	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1177	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1178	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1178	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1178	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1178	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1180	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1180	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1181	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1181	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1181	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1183	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1183	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1183	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1183	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1184	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1184	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1184	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1184	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1185	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1185	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1187	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1187	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1188	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1188	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1188	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1188	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1189	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1189	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1190	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1190	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1190	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1191	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1191	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1192	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1192	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1192	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1192	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1193	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1193	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1194	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1194	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1194	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1195	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1195	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1195	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1196	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1196	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1196	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1204	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1204	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1209	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1209	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1301	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1304	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1305	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1305	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1305	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1306	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1306	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1306	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1306	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1307	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1307	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1308	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1309	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1309	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-13100	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1311	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1311	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1311	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1311	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1314	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1314	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1314	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1317	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1318	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1318	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1318	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1318	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1319	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1320	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1321	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1322	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1324	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1324	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1326	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1326	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1332	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1335	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1342	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1342	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1342	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1342	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1344	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1344	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1346	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1347	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1347	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1348	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1349	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1350	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1350	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1350	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1351	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1352	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1353	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1354	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1355	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1356	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1356	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1358	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1358	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1359	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1360	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1360	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1362	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1362	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1362	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1363	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1363	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1364	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1368	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1369	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1370	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1372	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1375	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1375	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1378	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1379	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1380	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1381	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1382	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1382	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1383	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1384	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1385	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1386	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1387	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1389	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1391	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1392	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1393	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1398	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1403	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1406	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1406	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1412	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1412	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1415	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1415	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1415	TQLHKLNRAFQKEDS	8	23
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1415	ETKTQLHKLNRAFQK	5	20
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1417	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1417	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1420	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1420	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1421	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1422	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1425	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1427	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1427	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1429	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1429	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1430	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1433	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1434	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1437	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1437	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1440	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1452	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1453	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1453	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1465	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1467	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1473	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1473	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1474	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1474	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1477	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1478	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1478	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1480	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1480	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1481	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1483	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1483	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1484	KTQLHKLNRAFQKED	7	22
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1484	TKTQLHKLNRAFQKE	6	21
AK5-USP33	chr1	78001723	chr1	78167156	2568	DRB1-1485	KTQLHKLNRAFQKED	7	22

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Fusion breakpoint peptide structures of AK5-USP33

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
9448	TKTQLHKLNRAFQK	AK5	USP33	chr1	78001723	chr1	78167156	2568

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of AK5-USP33

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	9448	TKTQLHKLNRAFQK	-7.15543	-7.26883
HLA-B14:02	3BVN	9448	TKTQLHKLNRAFQK	-4.77435	-5.80965
HLA-B52:01	3W39	9448	TKTQLHKLNRAFQK	-6.80875	-6.92215
HLA-B52:01	3W39	9448	TKTQLHKLNRAFQK	-4.20386	-5.23916
HLA-A11:01	4UQ2	9448	TKTQLHKLNRAFQK	-7.5194	-8.5547
HLA-A11:01	4UQ2	9448	TKTQLHKLNRAFQK	-6.9601	-7.0735
HLA-A24:02	5HGA	9448	TKTQLHKLNRAFQK	-7.52403	-7.63743
HLA-A24:02	5HGA	9448	TKTQLHKLNRAFQK	-5.82433	-6.85963
HLA-B27:05	6PYJ	9448	TKTQLHKLNRAFQK	-3.28285	-4.31815
HLA-B44:05	3DX8	9448	TKTQLHKLNRAFQK	-5.91172	-6.94702
HLA-B44:05	3DX8	9448	TKTQLHKLNRAFQK	-4.24346	-4.35686

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Vaccine Design for the FusionNeoAntigens of AK5-USP33

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
AK5-USP33	chr1	78001723	chr1	78167156	10	18	LHKLNRAF	CTACACAAGCTTAACAGAGCGTTC
AK5-USP33	chr1	78001723	chr1	78167156	3	14	YYETKTQLHKL	TACTACGAGACAAAAACACAGCTACACAAGCTT
AK5-USP33	chr1	78001723	chr1	78167156	4	14	YETKTQLHKL	TACGAGACAAAAACACAGCTACACAAGCTT
AK5-USP33	chr1	78001723	chr1	78167156	5	14	ETKTQLHKL	GAGACAAAAACACAGCTACACAAGCTT
AK5-USP33	chr1	78001723	chr1	78167156	7	16	KTQLHKLNR	AAAACACAGCTACACAAGCTTAACAGA
AK5-USP33	chr1	78001723	chr1	78167156	8	18	TQLHKLNRAF	ACACAGCTACACAAGCTTAACAGAGCGTTC
AK5-USP33	chr1	78001723	chr1	78167156	9	18	QLHKLNRAF	CAGCTACACAAGCTTAACAGAGCGTTC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
AK5-USP33	chr1	78001723	chr1	78167156	5	20	ETKTQLHKLNRAFQK	GAGACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAA
AK5-USP33	chr1	78001723	chr1	78167156	6	21	TKTQLHKLNRAFQKE	ACAAAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAG
AK5-USP33	chr1	78001723	chr1	78167156	7	22	KTQLHKLNRAFQKED	AAAACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAGGAC
AK5-USP33	chr1	78001723	chr1	78167156	8	23	TQLHKLNRAFQKEDS	ACACAGCTACACAAGCTTAACAGAGCGTTCCAAAAAGAGGACTCT

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Information of the samples that have these potential fusion neoantigens of AK5-USP33

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
CESC	AK5-USP33	chr1	78001723	ENST00000344720	chr1	78167156	ENST00000357428	TCGA-WL-A834-01A

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Potential target of CAR-T therapy development for AK5-USP33

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to AK5-USP33

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to AK5-USP33

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)