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Fusion Protein:MARCH9-KCNC2

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MARCH9-KCNC2
	FusionPDB ID: 51716
	FusionGDB2.0 ID: 51716
		Hgene	Tgene
	Gene symbol	MARCH9	KCNC2
	Gene ID	92979	3747
	Gene name	membrane associated ring-CH-type finger 9	potassium voltage-gated channel subfamily C member 2
	Synonyms	MARCH-IX\|MARCH9\|RNF179	KV3.2
	Cytomap	12q14.1	12q21.1
	Type of gene	protein-coding	protein-coding
	Description	E3 ubiquitin-protein ligase MARCH9RING finger protein 179RING-type E3 ubiquitin transferase MARCH9membrane associated ring finger 9membrane-associated RING finger protein 9membrane-associated RING-CH protein IXmembrane-associated ring finger (C3HC4)	potassium voltage-gated channel subfamily C member 2potassium channel, voltage gated Shaw related subfamily C, member 2potassium voltage-gated channel, Shaw-related subfamily, member 2shaw-like potassium channelvoltage-gated potassium channel Kv3.2
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000266643, ENST00000548358,	ENST00000548243, ENST00000298972, ENST00000341669, ENST00000350228, ENST00000393288, ENST00000540018, ENST00000548513, ENST00000549446, ENST00000550433,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	9 X 6 X 6=324	15 X 5 X 6=450
	# samples	10	16
	** MAII score	log2(10/324*10)=-1.6959938131099 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(16/450*10)=-1.49185309632967 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: MARCH9 [Title/Abstract] AND KCNC2 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: MARCH9 [Title/Abstract] AND KCNC2 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MARCH9(58150867)-KCNC2(75445097), # samples:2
Anticipated loss of major functional domain due to fusion event.	MARCH9-KCNC2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MARCH9-KCNC2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MARCH9-KCNC2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MARCH9-KCNC2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:58150867/chr12:75445097)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across MARCH9 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across KCNC2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000550433	KCNC2	chr12	75445097	-	3167	944	14	2146	710
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000548513	KCNC2	chr12	75445097	-	3262	944	14	2098	694
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000549446	KCNC2	chr12	75445097	-	5201	944	14	2173	719
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000298972	KCNC2	chr12	75445097	-	3223	944	14	2098	694
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000341669	KCNC2	chr12	75445097	-	3128	944	14	2146	710
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000350228	KCNC2	chr12	75445097	-	3057	944	14	1933	639
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000540018	KCNC2	chr12	75445097	-	2153	944	14	2008	664
ENST00000266643	MARCH9	chr12	58150867	+	ENST00000393288	KCNC2	chr12	75445097	-	2494	944	14	2113	699

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000266643	ENST00000550433	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.004722146	0.9952779
ENST00000266643	ENST00000548513	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.004820602	0.9951794
ENST00000266643	ENST00000549446	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.003484172	0.99651575
ENST00000266643	ENST00000298972	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.005253531	0.9947464
ENST00000266643	ENST00000341669	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.005200595	0.9947994
ENST00000266643	ENST00000350228	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.002586724	0.99741334
ENST00000266643	ENST00000540018	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.026128424	0.9738715
ENST00000266643	ENST00000393288	MARCH9	chr12	58150867	+	KCNC2	chr12	75445097	-	0.01265955	0.9873404

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for MARCH9-KCNC2

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
MARCH9	chr12	58150867	KCNC2	chr12	75445097	944	307	YFKYQVLAISTKNPLQFIAFASLFFI

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Potential FusionNeoAntigen Information of MARCH9-KCNC2 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

MARCH9-KCNC2_58150867_75445097.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:01	NPLQFIAF	0.9736	0.8115	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:01	ISTKNPLQF	0.9989	0.9775	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B58:02	ISTKNPLQF	0.9976	0.9327	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:03	ISTKNPLQF	0.9965	0.9748	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:17	ISTKNPLQF	0.9953	0.9439	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B58:01	ISTKNPLQF	0.9943	0.9101	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:16	ISTKNPLQF	0.9941	0.7078	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B39:06	TKNPLQFIA	0.9921	0.7595	10	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:16	STKNPLQFI	0.9875	0.666	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-A30:08	STKNPLQFI	0.9855	0.7574	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-A32:13	ISTKNPLQF	0.693	0.9372	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B58:02	AISTKNPLQF	0.987	0.949	7	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-A30:08	STKNPLQFIA	0.9866	0.7702	9	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:03	AISTKNPLQF	0.984	0.984	7	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:03	TKNPLQFIAF	0.9591	0.8753	10	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:17	LAISTKNPLQF	0.9998	0.9276	6	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:03	LAISTKNPLQF	0.9998	0.9812	6	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:16	LAISTKNPLQF	0.9995	0.813	6	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B58:01	LAISTKNPLQF	0.9994	0.9107	6	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:04	STKNPLQF	0.9989	0.8814	9	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B42:02	NPLQFIAF	0.9335	0.6411	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:04	STKNPLQFI	0.9986	0.8961	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:06	STKNPLQFI	0.9979	0.9362	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:08	ISTKNPLQF	0.9946	0.9362	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:19	ISTKNPLQF	0.9892	0.9914	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:04	ISTKNPLQF	0.9858	0.8952	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:07	ISTKNPLQF	0.9696	0.9773	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:06	ISTKNPLQF	0.9595	0.937	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B73:01	TKNPLQFIA	0.9501	0.5595	10	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C04:06	ISTKNPLQF	0.8818	0.9257	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C06:03	STKNPLQFI	0.8624	0.9892	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C12:04	STKNPLQFI	0.8489	0.9932	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:14	ISTKNPLQF	0.7635	0.9813	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C12:12	STKNPLQFI	0.7608	0.8816	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C12:12	ISTKNPLQF	0.7154	0.8928	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C08:13	ISTKNPLQF	0.5885	0.9446	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C08:04	ISTKNPLQF	0.5885	0.9446	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C02:06	STKNPLQFI	0.4962	0.9693	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C12:04	ISTKNPLQF	0.4688	0.9922	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C06:03	ISTKNPLQF	0.464	0.9883	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C08:03	ISTKNPLQF	0.1351	0.9796	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C02:06	ISTKNPLQF	0.0604	0.9461	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:09	STKNPLQF	0.9989	0.8814	9	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C16:01	STKNPLQF	0.9949	0.9821	9	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C16:02	STKNPLQF	0.9904	0.9935	9	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:77	NPLQFIAF	0.9736	0.8115	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:23	NPLQFIAF	0.9729	0.7942	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:11	NPLQFIAF	0.972	0.8291	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:17	NPLQFIAF	0.9687	0.6573	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:30	NPLQFIAF	0.9687	0.6573	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B35:24	NPLQFIAF	0.9521	0.7635	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B18:07	NPLQFIAF	0.8168	0.76	12	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:10	ISTKNPLQF	0.9989	0.9775	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:02	STKNPLQFI	0.9987	0.8924	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:09	STKNPLQFI	0.9986	0.8961	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:05	STKNPLQFI	0.9983	0.9043	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B58:06	ISTKNPLQF	0.9975	0.7567	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:02	ISTKNPLQF	0.9975	0.9789	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:04	ISTKNPLQF	0.9974	0.6125	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:04	ISTKNPLQF	0.9956	0.992	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:03	ISTKNPLQF	0.9956	0.992	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:02	ISTKNPLQF	0.989	0.7839	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-A30:01	STKNPLQFI	0.9872	0.8413	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:09	ISTKNPLQF	0.9858	0.8952	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:17	ISTKNPLQF	0.9766	0.9797	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:05	ISTKNPLQF	0.9738	0.9622	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C06:06	ISTKNPLQF	0.9556	0.9868	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C16:04	ISTKNPLQF	0.9415	0.9698	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C16:02	STKNPLQFI	0.9202	0.993	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:24	ISTKNPLQF	0.9168	0.9546	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:05	ISTKNPLQF	0.8996	0.9393	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C15:02	ISTKNPLQF	0.874	0.9209	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-A69:01	STKNPLQFI	0.8233	0.8748	9	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C12:02	ISTKNPLQF	0.7671	0.9656	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C12:03	ISTKNPLQF	0.756	0.9704	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C16:01	ISTKNPLQF	0.6942	0.9828	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C03:06	ISTKNPLQF	0.6264	0.9912	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C16:02	ISTKNPLQF	0.5662	0.9906	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C08:01	ISTKNPLQF	0.1351	0.9796	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C02:02	ISTKNPLQF	0.1305	0.9588	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-C02:10	ISTKNPLQF	0.1305	0.9588	8	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:02	AISTKNPLQF	0.9907	0.9468	7	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-A30:01	STKNPLQFIA	0.9858	0.8675	9	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:68	TKNPLQFIAF	0.9662	0.6917	10	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B15:54	TKNPLQFIAF	0.9566	0.9289	10	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B48:02	TKNPLQFIAF	0.9139	0.9503	10	20
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	HLA-B57:02	LAISTKNPLQF	0.9997	0.9421	6	17

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Potential FusionNeoAntigen Information of MARCH9-KCNC2 in HLA II

MARCH9-KCNC2_58150867_75445097.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1117	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1117	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1117	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1117	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1152	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1152	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1152	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1152	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1189	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1189	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1189	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1331	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1331	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1377	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1377	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1401	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1401	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1401	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1401	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1404	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1404	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1404	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1404	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1405	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1405	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1405	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1405	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1407	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1407	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1407	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1407	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1408	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1408	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1408	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1408	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1411	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1411	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1411	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1411	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1414	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1414	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1414	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1418	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1418	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1418	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1418	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1423	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1423	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1423	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1423	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1426	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1426	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1426	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1426	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1428	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1428	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1428	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1428	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1431	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1431	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1431	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1432	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1432	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1432	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1432	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1433	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1434	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1434	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1434	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1435	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1435	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1435	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1435	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1436	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1436	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1436	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1438	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1438	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1438	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1438	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1439	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1439	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1439	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1439	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1442	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1442	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1442	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1443	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1443	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1443	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1443	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1444	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1444	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1444	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1445	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1445	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1445	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1445	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1449	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1449	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1450	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1450	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1450	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1450	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1454	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1454	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1454	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1454	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1455	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1455	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1455	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1455	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1456	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1456	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1456	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1456	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1458	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1458	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1458	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1458	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1459	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1459	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1459	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1459	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1460	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1460	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1460	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1460	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1461	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1461	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1461	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1461	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1462	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1462	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1462	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1462	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1462	YFKYQVLAISTKNPL	0	15
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1464	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1464	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1464	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1464	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1465	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1465	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1465	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1468	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1468	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1468	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1468	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1470	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1470	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1470	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1470	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1471	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1471	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1471	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1471	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1472	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1472	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1472	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1472	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1473	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1474	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1475	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1475	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1475	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1475	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1481	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1481	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1482	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1482	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1482	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1482	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1482	YFKYQVLAISTKNPL	0	15
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1486	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1486	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1486	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1486	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1487	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1487	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1487	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1487	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1488	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1488	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1488	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1488	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1490	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1490	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1490	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1490	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1491	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1491	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1491	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1491	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1493	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1493	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1493	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1493	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1495	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1495	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1495	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1495	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1496	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1496	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1496	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1496	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1497	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1497	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1497	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1497	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1499	KYQVLAISTKNPLQF	2	17
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1499	YQVLAISTKNPLQFI	3	18
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1499	FKYQVLAISTKNPLQ	1	16
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1499	QVLAISTKNPLQFIA	4	19
MARCH9-KCNC2	chr12	58150867	chr12	75445097	944	DRB1-1499	YFKYQVLAISTKNPL	0	15

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Fusion breakpoint peptide structures of MARCH9-KCNC2

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
4749	LAISTKNPLQFIAF	MARCH9	KCNC2	chr12	58150867	chr12	75445097	944

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of MARCH9-KCNC2

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B53:01	1A1O	4749	LAISTKNPLQFIAF	-4.17128	-4.28468
HLA-B53:01	1A1O	4749	LAISTKNPLQFIAF	-3.4824	-4.5177
HLA-B51:01	1E28	4749	LAISTKNPLQFIAF	-3.8039	-3.9173
HLA-B51:01	1E28	4749	LAISTKNPLQFIAF	-3.0982	-4.1335
HLA-B57:03	2BVO	4749	LAISTKNPLQFIAF	-2.25252	-3.28782
HLA-B57:03	2BVO	4749	LAISTKNPLQFIAF	-1.1301	-1.2435
HLA-A03:01	2XPG	4749	LAISTKNPLQFIAF	-4.44786	-4.56126
HLA-A03:01	2XPG	4749	LAISTKNPLQFIAF	-4.14835	-5.18365
HLA-B14:02	3BVN	4749	LAISTKNPLQFIAF	-5.80238	-5.91578
HLA-B14:02	3BVN	4749	LAISTKNPLQFIAF	-3.55223	-4.58753
HLA-B52:01	3W39	4749	LAISTKNPLQFIAF	-5.11856	-5.23196
HLA-B52:01	3W39	4749	LAISTKNPLQFIAF	-3.25922	-4.29452
HLA-B18:01	4JQV	4749	LAISTKNPLQFIAF	-4.41315	-4.52655
HLA-B18:01	4JQV	4749	LAISTKNPLQFIAF	-3.36095	-4.39625
HLA-A11:01	4UQ2	4749	LAISTKNPLQFIAF	-8.56989	-8.68329
HLA-A11:01	4UQ2	4749	LAISTKNPLQFIAF	-6.72556	-7.76086
HLA-A24:02	5HGA	4749	LAISTKNPLQFIAF	-6.2734	-6.3868
HLA-A24:02	5HGA	4749	LAISTKNPLQFIAF	-4.24193	-5.27723
HLA-B57:01	5VUD	4749	LAISTKNPLQFIAF	-3.95679	-4.99209
HLA-B57:01	5VUD	4749	LAISTKNPLQFIAF	-2.97944	-3.09284
HLA-C08:02	6JTP	4749	LAISTKNPLQFIAF	-4.74128	-4.85468
HLA-C08:02	6JTP	4749	LAISTKNPLQFIAF	-2.44773	-3.48303
HLA-B37:01	6MT4	4749	LAISTKNPLQFIAF	-3.77829	-4.81359
HLA-B27:05	6PYJ	4749	LAISTKNPLQFIAF	-3.40019	-3.51359
HLA-B27:05	6PYJ	4749	LAISTKNPLQFIAF	-1.99024	-3.02554
HLA-B27:03	6PZ5	4749	LAISTKNPLQFIAF	-3.1249	-3.2383
HLA-B27:03	6PZ5	4749	LAISTKNPLQFIAF	-0.148491	-1.18379
HLA-B44:05	3DX8	4749	LAISTKNPLQFIAF	-5.76333	-5.87673
HLA-B44:05	3DX8	4749	LAISTKNPLQFIAF	-3.98513	-5.02043
HLA-B44:02	1M6O	4749	LAISTKNPLQFIAF	-1.76802	-2.80332
HLA-B07:02	5EO0	4749	LAISTKNPLQFIAF	-3.47003	-3.58343
HLA-B07:02	5EO0	4749	LAISTKNPLQFIAF	0.983323	-0.0519774
HLA-A02:01	6TDR	4749	LAISTKNPLQFIAF	-2.71064	-3.74594
HLA-A02:01	6TDR	4749	LAISTKNPLQFIAF	-2.80613	-2.91953

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Vaccine Design for the FusionNeoAntigens of MARCH9-KCNC2

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
MARCH9-KCNC2	chr12	58150867	chr12	75445097	10	19	TKNPLQFIA	CCACTGCAGTTTATTGCTTTTGCTTCT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	10	20	TKNPLQFIAF	CCACTGCAGTTTATTGCTTTTGCTTCTTTA
MARCH9-KCNC2	chr12	58150867	chr12	75445097	12	20	NPLQFIAF	CAGTTTATTGCTTTTGCTTCTTTA
MARCH9-KCNC2	chr12	58150867	chr12	75445097	6	17	LAISTKNPLQF	AGCACCAAGAACCCACTGCAGTTTATTGCTTTT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	7	17	AISTKNPLQF	ACCAAGAACCCACTGCAGTTTATTGCTTTT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	8	17	ISTKNPLQF	AAGAACCCACTGCAGTTTATTGCTTTT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	9	17	STKNPLQF	AACCCACTGCAGTTTATTGCTTTT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	9	18	STKNPLQFI	AACCCACTGCAGTTTATTGCTTTTGCT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	9	19	STKNPLQFIA	AACCCACTGCAGTTTATTGCTTTTGCTTCT

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
MARCH9-KCNC2	chr12	58150867	chr12	75445097	0	15	YFKYQVLAISTKNPL	TACCAGGTCCTGGCGATCAGCACCAAGAACCCACTGCAGTTTATT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	1	16	FKYQVLAISTKNPLQ	CAGGTCCTGGCGATCAGCACCAAGAACCCACTGCAGTTTATTGCT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	2	17	KYQVLAISTKNPLQF	GTCCTGGCGATCAGCACCAAGAACCCACTGCAGTTTATTGCTTTT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	3	18	YQVLAISTKNPLQFI	CTGGCGATCAGCACCAAGAACCCACTGCAGTTTATTGCTTTTGCT
MARCH9-KCNC2	chr12	58150867	chr12	75445097	4	19	QVLAISTKNPLQFIA	GCGATCAGCACCAAGAACCCACTGCAGTTTATTGCTTTTGCTTCT

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Information of the samples that have these potential fusion neoantigens of MARCH9-KCNC2

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
BLCA	MARCH9-KCNC2	chr12	58150867	ENST00000266643	chr12	75445097	ENST00000298972	TCGA-BT-A20X-01A

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Potential target of CAR-T therapy development for MARCH9-KCNC2

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000298972

230_250

599.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000298972

284_303

599.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000298972

314_334

599.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000298972

346_368

599.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000298972

382_402

599.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000298972

453_473

599.0

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000341669

230_250

630.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000341669

284_303

630.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000341669

314_334

630.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000341669

346_368

630.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000341669

382_402

630.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000341669

453_473

630.0

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000350228

230_250

543.6666666666666

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000350228

284_303

543.6666666666666

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000350228

314_334

543.6666666666666

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000350228

346_368

543.6666666666666

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000350228

382_402

543.6666666666666

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000350228

453_473

543.6666666666666

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000393288

230_250

619.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000393288

284_303

619.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000393288

314_334

619.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000393288

346_368

619.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000393288

382_402

619.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000393288

453_473

619.0

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000540018

230_250

584.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000540018

284_303

584.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000540018

314_334

584.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000540018

346_368

584.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000540018

382_402

584.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000540018

453_473

584.0

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000548513

230_250

477.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000548513

284_303

477.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000548513

314_334

477.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000548513

346_368

477.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000548513

382_402

477.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000548513

453_473

477.0

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000549446

230_250

639.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000549446

284_303

639.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000549446

314_334

639.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000549446

346_368

639.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000549446

382_402

639.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000549446

453_473

639.0

Transmembrane

Helical%3B Name%3DSegment S6

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000550433

230_250

630.0

Transmembrane

Helical%3B Name%3DSegment S1

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000550433

284_303

630.0

Transmembrane

Helical%3B Name%3DSegment S2

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000550433

314_334

630.0

Transmembrane

Helical%3B Name%3DSegment S3

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000550433

346_368

630.0

Transmembrane

Helical%3B Voltage-sensor%3B Name%3DSegment S4

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000550433

382_402

630.0

Transmembrane

Helical%3B Name%3DSegment S5

Tgene

KCNC2

chr12:58150867

chr12:75445097

ENST00000550433

453_473

630.0

Transmembrane

Helical%3B Name%3DSegment S6

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to MARCH9-KCNC2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to MARCH9-KCNC2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)