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Fusion Protein:AAK1-RPIA

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: AAK1-RPIA
	FusionPDB ID: 68
	FusionGDB2.0 ID: 68
		Hgene	Tgene
	Gene symbol	AAK1	RPIA
	Gene ID	112268437	22934
	Gene name	uncharacterized protein FLJ45252	ribose 5-phosphate isomerase A
	Synonyms	AAK1	RPI\|RPIAD
	Cytomap	-	2p11.2
	Type of gene	protein-coding	protein-coding
	Description	uncharacterized protein FLJ45252alternative protein AAK1	ribose-5-phosphate isomerasephosphoriboisomeraseribose 5-phosphate epimerase
	Modification date	20200303	20200327
	UniProtAcc	Q2M2I8 Main function of 5'-partner protein: FUNCTION: Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:17494869, PubMed:11877457, PubMed:11877461, PubMed:12952931, PubMed:14617351, PubMed:25653444). Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1 (PubMed:17494869). Preferentially, may phosphorylate substrates on threonine residues (PubMed:11877457, PubMed:18657069). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (PubMed:12952931). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (PubMed:18657069). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (PubMed:21464124). {ECO:0000269\|PubMed:11877457, ECO:0000269\|PubMed:11877461, ECO:0000269\|PubMed:12952931, ECO:0000269\|PubMed:14617351, ECO:0000269\|PubMed:17494869, ECO:0000269\|PubMed:18657069, ECO:0000269\|PubMed:21464124, ECO:0000269\|PubMed:25653444}.; FUNCTION: (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue. {ECO:0000269\|PubMed:25653444, ECO:0000305\|PubMed:31136173}.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000406297, ENST00000409068, ENST00000409085, ENST00000470281,	ENST00000283646,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	19 X 13 X 11=2717	4 X 3 X 3=36
	# samples	22	4
	** MAII score	log2(22/2717*10)=-3.62643913669732 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/36*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Fusion gene context	PubMed: AAK1 [Title/Abstract] AND RPIA [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: AAK1 [Title/Abstract] AND RPIA [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	AAK1(69783992)-RPIA(89028796), # samples:2
Anticipated loss of major functional domain due to fusion event.	AAK1-RPIA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. AAK1-RPIA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. AAK1-RPIA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. AAK1-RPIA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:69783992/chr2:89028796)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across AAK1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across RPIA (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000409068	AAK1	chr2	69783992	-	ENST00000283646	RPIA	chr2	89028796	+	1987	596	215	1129	304
ENST00000409085	AAK1	chr2	69783992	-	ENST00000283646	RPIA	chr2	89028796	+	2050	659	278	1192	304
ENST00000406297	AAK1	chr2	69783992	-	ENST00000283646	RPIA	chr2	89028796	+	2050	659	278	1192	304

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000409068	ENST00000283646	AAK1	chr2	69783992	-	RPIA	chr2	89028796	+	0.000859454	0.9991405
ENST00000409085	ENST00000283646	AAK1	chr2	69783992	-	RPIA	chr2	89028796	+	0.0010715	0.9989285
ENST00000406297	ENST00000283646	AAK1	chr2	69783992	-	RPIA	chr2	89028796	+	0.0010715	0.9989285

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for AAK1-RPIA

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
AAK1	chr2	69783992	RPIA	chr2	89028796	596	127	HDLQVCKREIQIMARQLILQYGLTLS
AAK1	chr2	69783992	RPIA	chr2	89028796	659	127	HDLQVCKREIQIMARQLILQYGLTLS

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Potential FusionNeoAntigen Information of AAK1-RPIA in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

AAK1-RPIA_69783992_89028796.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B52:01	IQIMARQL	0.7947	0.6813	9	17
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:01	MARQLILQY	0.9996	0.8541	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:25	MARQLILQY	0.9995	0.9138	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:02	MARQLILQY	0.9988	0.9201	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:17	MARQLILQY	0.9979	0.8401	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B08:01	QIMARQLIL	0.9967	0.9329	10	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:08	MARQLILQY	0.9966	0.9399	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B57:01	MARQLILQY	0.9961	0.9458	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B08:09	QIMARQLIL	0.9941	0.8024	10	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B27:05	KREIQIMAR	0.994	0.7649	6	15
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:01	MARQLILQY	0.9904	0.9259	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B58:02	MARQLILQY	0.9888	0.9071	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:16	MARQLILQY	0.9869	0.7074	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:05	MARQLILQY	0.9769	0.5869	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:03	MARQLILQY	0.9702	0.7577	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B58:01	MARQLILQY	0.9662	0.8142	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B13:01	IQIMARQLI	0.5874	0.9402	9	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-A32:13	MARQLILQY	0.445	0.8371	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B52:01	IQIMARQLI	0.3986	0.8054	9	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:01	IMARQLILQY	0.9999	0.8013	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:25	IMARQLILQY	0.997	0.8642	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B44:03	REIQIMARQL	0.9729	0.9432	7	17
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:21	MARQLILQY	0.9989	0.9289	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:07	MARQLILQY	0.9985	0.5802	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:05	MARQLILQY	0.9962	0.8826	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B27:14	KREIQIMAR	0.9936	0.6497	6	15
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:31	MARQLILQY	0.9901	0.8904	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C07:19	MARQLILQY	0.9705	0.5963	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C12:16	MARQLILQY	0.9394	0.9588	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C07:95	MARQLILQY	0.9391	0.6167	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:04	MARQLILQY	0.9073	0.8611	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C15:04	MARQLILQY	0.8549	0.7695	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C07:05	MARQLILQY	0.8326	0.9534	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C03:14	MARQLILQY	0.7203	0.9187	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B27:03	KREIQIMAR	0.7057	0.7887	6	15
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C12:12	MARQLILQY	0.6476	0.9479	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C12:04	MARQLILQY	0.5935	0.9874	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C06:03	MARQLILQY	0.5856	0.9901	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B51:07	IQIMARQLI	0.3629	0.7029	9	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:07	IMARQLILQY	0.9997	0.5737	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:04	IMARQLILQY	0.994	0.8391	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:33	MARQLILQY	0.9996	0.8541	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:125	MARQLILQY	0.9996	0.8541	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:34	MARQLILQY	0.9996	0.8541	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:135	MARQLILQY	0.9995	0.854	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:27	MARQLILQY	0.9995	0.8532	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:39	MARQLILQY	0.9994	0.8289	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:08	MARQLILQY	0.999	0.79	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:11	MARQLILQY	0.999	0.7834	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:11	MARQLILQY	0.9986	0.9281	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:50	MARQLILQY	0.9985	0.8981	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:43	MARQLILQY	0.9985	0.801	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:35	MARQLILQY	0.9983	0.7464	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:24	MARQLILQY	0.9979	0.7598	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:12	MARQLILQY	0.997	0.7838	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B57:04	MARQLILQY	0.9968	0.7133	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B08:18	QIMARQLIL	0.9967	0.9329	10	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:53	MARQLILQY	0.9962	0.8288	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B57:10	MARQLILQY	0.9961	0.9458	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:20	MARQLILQY	0.9961	0.9298	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:28	MARQLILQY	0.9951	0.9308	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B58:06	MARQLILQY	0.9934	0.7735	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:30	MARQLILQY	0.9918	0.8245	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:17	MARQLILQY	0.9918	0.8245	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:23	MARQLILQY	0.9912	0.9265	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B27:10	KREIQIMAR	0.9909	0.8524	6	15
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:20	MARQLILQY	0.9909	0.9428	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:77	MARQLILQY	0.9904	0.9259	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:13	MARQLILQY	0.9872	0.5408	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B35:24	MARQLILQY	0.9803	0.9134	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B08:12	QIMARQLIL	0.9673	0.9554	10	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:54	MARQLILQY	0.9607	0.8092	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C07:17	MARQLILQY	0.9576	0.963	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C03:02	MARQLILQY	0.9541	0.967	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C07:01	MARQLILQY	0.9445	0.6071	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B48:02	MARQLILQY	0.9393	0.92	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-A30:01	MARQLILQY	0.9174	0.7456	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C15:09	MARQLILQY	0.8549	0.7695	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:68	MARQLILQY	0.8402	0.5196	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C12:02	MARQLILQY	0.8245	0.963	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B18:04	MARQLILQY	0.7642	0.9014	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C12:03	MARQLILQY	0.7402	0.977	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C16:04	MARQLILQY	0.6706	0.98	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-A25:01	MARQLILQY	0.6499	0.8206	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C16:01	MARQLILQY	0.5282	0.9712	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C02:02	MARQLILQY	0.5109	0.9561	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C02:10	MARQLILQY	0.5109	0.9561	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B18:07	MARQLILQY	0.4693	0.8446	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B18:08	MARQLILQY	0.4627	0.8181	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-C16:02	MARQLILQY	0.4289	0.9858	12	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:27	IMARQLILQY	0.9999	0.8183	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:33	IMARQLILQY	0.9999	0.8013	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:135	IMARQLILQY	0.9999	0.8257	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:34	IMARQLILQY	0.9999	0.8013	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:125	IMARQLILQY	0.9999	0.8013	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:35	IMARQLILQY	0.9996	0.7473	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B15:39	IMARQLILQY	0.9966	0.7543	11	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B40:04	REIQIMARQL	0.9943	0.6833	7	17
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B44:26	REIQIMARQL	0.9729	0.9432	7	17
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B44:07	REIQIMARQL	0.9729	0.9432	7	17
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B44:13	REIQIMARQL	0.9729	0.9432	7	17
AAK1-RPIA	chr2	69783992	chr2	89028796	659	HLA-B41:03	REIQIMARQL	0.7066	0.7468	7	17

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Potential FusionNeoAntigen Information of AAK1-RPIA in HLA II

AAK1-RPIA_69783992_89028796.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1113	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1113	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1113	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1113	REIQIMARQLILQYG	7	22
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1117	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1117	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1117	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1118	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1118	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1134	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1134	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1142	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1142	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1152	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1152	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1152	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1157	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1157	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1184	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1184	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1189	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1189	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1189	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1192	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1192	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1203	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1203	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1204	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1204	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1209	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1209	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1306	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1306	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1309	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1309	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-13100	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1331	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1331	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1344	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1344	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1346	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1361	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1361	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1377	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1377	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1377	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1386	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1401	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1401	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1401	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1401	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1404	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1404	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1404	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1405	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1405	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1405	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1406	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1406	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1407	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1407	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1408	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1408	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1408	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1411	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1411	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1411	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1414	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1414	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1416	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1416	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1417	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1417	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1418	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1418	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1418	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1420	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1420	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1421	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1421	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1423	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1423	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1423	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1426	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1426	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1426	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1426	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1428	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1428	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1428	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1428	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1429	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1429	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1430	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1430	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1431	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1431	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1431	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1431	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1432	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1432	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1432	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1432	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1433	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1433	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1434	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1434	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1434	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1435	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1435	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1435	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1435	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1436	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1436	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1437	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1437	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1438	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1438	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1438	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1439	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1439	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1442	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1442	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1443	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1443	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1443	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1444	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1444	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1445	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1445	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1445	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1449	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1450	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1450	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1450	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1452	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1452	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1454	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1454	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1454	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1454	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1455	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1455	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1455	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1456	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1456	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1456	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1458	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1458	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1458	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1458	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1459	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1459	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1459	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1460	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1460	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1460	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1460	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1461	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1461	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1461	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1462	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1462	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1464	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1464	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1464	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1465	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1465	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1465	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1468	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1468	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1470	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1470	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1471	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1471	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1471	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1472	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1472	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1472	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1474	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1474	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1475	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1475	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1475	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1480	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1480	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1481	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1481	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1481	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1482	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1482	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1482	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1483	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1483	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1486	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1486	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1486	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1486	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1487	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1487	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1487	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1487	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1488	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1488	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1488	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1488	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1490	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1490	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1490	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1490	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1491	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1491	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1491	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1493	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1493	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1495	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1495	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1495	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1496	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1496	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1496	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1497	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1497	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1497	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1497	QVCKREIQIMARQLI	3	18
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1499	CKREIQIMARQLILQ	5	20
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1499	KREIQIMARQLILQY	6	21
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1499	VCKREIQIMARQLIL	4	19
AAK1-RPIA	chr2	69783992	chr2	89028796	659	DRB1-1499	QVCKREIQIMARQLI	3	18

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Fusion breakpoint peptide structures of AAK1-RPIA

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
4552	KREIQIMARQLILQ	AAK1	RPIA	chr2	69783992	chr2	89028796	659

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of AAK1-RPIA

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	4552	KREIQIMARQLILQ	-7.9962	-8.1096
HLA-B14:02	3BVN	4552	KREIQIMARQLILQ	-5.70842	-6.74372
HLA-B52:01	3W39	4552	KREIQIMARQLILQ	-6.83737	-6.95077
HLA-B52:01	3W39	4552	KREIQIMARQLILQ	-4.4836	-5.5189
HLA-A11:01	4UQ2	4552	KREIQIMARQLILQ	-10.0067	-10.1201
HLA-A11:01	4UQ2	4552	KREIQIMARQLILQ	-9.03915	-10.0745
HLA-A24:02	5HGA	4552	KREIQIMARQLILQ	-6.56204	-6.67544
HLA-A24:02	5HGA	4552	KREIQIMARQLILQ	-5.42271	-6.45801
HLA-B44:05	3DX8	4552	KREIQIMARQLILQ	-7.85648	-8.89178
HLA-B44:05	3DX8	4552	KREIQIMARQLILQ	-5.3978	-5.5112
HLA-A02:01	6TDR	4552	KREIQIMARQLILQ	-3.37154	-4.40684

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Vaccine Design for the FusionNeoAntigens of AAK1-RPIA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
AAK1-RPIA	chr2	69783992	chr2	89028796	10	19	QIMARQLIL	CAGATAATGGCCCGCCAGCTCATCCTG
AAK1-RPIA	chr2	69783992	chr2	89028796	11	21	IMARQLILQY	ATAATGGCCCGCCAGCTCATCCTGCAGTAT
AAK1-RPIA	chr2	69783992	chr2	89028796	12	21	MARQLILQY	ATGGCCCGCCAGCTCATCCTGCAGTAT
AAK1-RPIA	chr2	69783992	chr2	89028796	6	15	KREIQIMAR	AAGAGAGAAATCCAGATAATGGCCCGC
AAK1-RPIA	chr2	69783992	chr2	89028796	7	17	REIQIMARQL	AGAGAAATCCAGATAATGGCCCGCCAGCTC
AAK1-RPIA	chr2	69783992	chr2	89028796	9	17	IQIMARQL	ATCCAGATAATGGCCCGCCAGCTC
AAK1-RPIA	chr2	69783992	chr2	89028796	9	18	IQIMARQLI	ATCCAGATAATGGCCCGCCAGCTCATC

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
AAK1-RPIA	chr2	69783992	chr2	89028796	3	18	QVCKREIQIMARQLI	CAGGTGTGCAAGAGAGAAATCCAGATAATGGCCCGCCAGCTCATC
AAK1-RPIA	chr2	69783992	chr2	89028796	4	19	VCKREIQIMARQLIL	GTGTGCAAGAGAGAAATCCAGATAATGGCCCGCCAGCTCATCCTG
AAK1-RPIA	chr2	69783992	chr2	89028796	5	20	CKREIQIMARQLILQ	TGCAAGAGAGAAATCCAGATAATGGCCCGCCAGCTCATCCTGCAG
AAK1-RPIA	chr2	69783992	chr2	89028796	6	21	KREIQIMARQLILQY	AAGAGAGAAATCCAGATAATGGCCCGCCAGCTCATCCTGCAGTAT
AAK1-RPIA	chr2	69783992	chr2	89028796	7	22	REIQIMARQLILQYG	AGAGAAATCCAGATAATGGCCCGCCAGCTCATCCTGCAGTATGGC

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Information of the samples that have these potential fusion neoantigens of AAK1-RPIA

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
LIHC	AAK1-RPIA	chr2	69783992	ENST00000406297	chr2	89028796	ENST00000283646	TCGA-DD-A3A5-01A

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Potential target of CAR-T therapy development for AAK1-RPIA

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to AAK1-RPIA

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to AAK1-RPIA

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)