Home

Download

Statistics

Examples

Help

Contact

Fusion Protein:RASAL2-KIF14

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RASAL2-KIF14
	FusionPDB ID: 72423
	FusionGDB2.0 ID: 72423
		Hgene	Tgene
	Gene symbol	RASAL2	KIF14
	Gene ID	9462	9928
	Gene name	RAS protein activator like 2	kinesin family member 14
	Synonyms	NGAP	MCPH20\|MKS12
	Cytomap	1q25.2	1q32.1
	Type of gene	protein-coding	protein-coding
	Description	ras GTPase-activating protein nGAPRASAL2/ACVR1 fusionRas protein activator like 1	kinesin-like protein KIF14epididymis secretory sperm binding protein
	Modification date	20200313	20200315
	UniProtAcc	.	Q15058 Main function of 5'-partner protein: FUNCTION: Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity (By similarity). Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis (PubMed:24784001, PubMed:16648480). During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively (PubMed:16431929). Regulates cell growth through regulation of cell cycle progression and cytokinesis (PubMed:24854087). During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1 (PubMed:24854087). During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division (By similarity). Also is required for chromosome congression and alignment during mitotic cell cycle process (PubMed:15843429). Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules (PubMed:23209302). {ECO:0000250\|UniProtKB:L0N7N1, ECO:0000269\|PubMed:15843429, ECO:0000269\|PubMed:16431929, ECO:0000269\|PubMed:16648480, ECO:0000269\|PubMed:23209302, ECO:0000269\|PubMed:24784001, ECO:0000269\|PubMed:24854087}.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000465723, ENST00000367649, ENST00000448150, ENST00000462775,	ENST00000367350,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	19 X 13 X 10=2470	3 X 3 X 3=27
	# samples	20	4
	** MAII score	log2(20/2470*10)=-3.62643913669732 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/27*10)=0.567040592723894 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Fusion gene context	PubMed: RASAL2 [Title/Abstract] AND KIF14 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: RASAL2 [Title/Abstract] AND KIF14 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	RASAL2(178252826)-KIF14(200544819), # samples:3
Anticipated loss of major functional domain due to fusion event.	RASAL2-KIF14 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RASAL2-KIF14 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	KIF14	GO:0008284	positive regulation of cell proliferation	24784001
Tgene	KIF14	GO:0034446	substrate adhesion-dependent cell spreading	23209302
Tgene	KIF14	GO:0045184	establishment of protein localization	23209302

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:178252826/chr1:200544819)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across RASAL2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across KIF14 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000448150

RASAL2

chr1

178252826

ENST00000367350

KIF14

chr1

200544819

4464

1094

410

2575

721

ENST00000367649

RASAL2

chr1

178252826

ENST00000367350

KIF14

chr1

200544819

4052

682

166

2163

665

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000448150	ENST00000367350	RASAL2	chr1	178252826	+	KIF14	chr1	200544819	-	0.000447484	0.99955255
ENST00000367649	ENST00000367350	RASAL2	chr1	178252826	+	KIF14	chr1	200544819	-	0.000291167	0.99970883

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

Top

Fusion Protein Breakpoint Sequences for RASAL2-KIF14

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
RASAL2	chr1	178252826	KIF14	chr1	200544819	1094	228	TDSQLVLLNKEKESNGSNRGEDAFCD
RASAL2	chr1	178252826	KIF14	chr1	200544819	682	172	TDSQLVLLNKEKESNGSNRGEDAFCD

Top

Potential FusionNeoAntigen Information of RASAL2-KIF14 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene

Hchr

Hbp

Tgene

Tchr

Tbp

HLA I

FusionNeoAntigen peptide

Binding score

Immunogenic score

Neoantigen start (at BP 13)

Neoantigen end (at BP 13)

Top

Potential FusionNeoAntigen Information of RASAL2-KIF14 in HLA II

RASAL2-KIF14_178252826_200544819.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0801	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0801	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0801	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0803	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0803	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0804	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0804	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0805	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0805	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0805	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0806	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0806	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0806	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0808	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0808	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0810	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0810	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0810	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0811	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0811	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0812	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0812	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0812	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0813	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0814	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0814	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0816	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0816	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0816	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0818	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0818	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0818	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0820	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0820	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0822	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0822	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0822	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0823	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0823	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0824	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0824	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0826	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0826	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0826	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0827	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0827	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0828	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0828	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0829	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0829	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0831	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0831	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0831	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0832	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0832	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0833	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0833	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0835	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0835	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0836	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0836	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0837	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0837	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0838	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0838	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0839	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0839	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0839	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0840	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0840	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-0840	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1101	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1101	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1103	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1104	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1104	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1104	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1105	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1105	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1106	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1106	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1106	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1108	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1108	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1109	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1109	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1110	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1110	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1112	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1112	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1113	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1113	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1115	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1115	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1118	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1118	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1118	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1119	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1119	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1124	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1124	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1125	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1125	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1127	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1128	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1128	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1129	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1129	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1131	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1131	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1132	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1133	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1133	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1134	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1134	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1134	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1135	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1135	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1135	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1137	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1137	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1138	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1138	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1138	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1139	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1139	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1141	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1142	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1142	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1142	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1143	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1143	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1143	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1144	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1144	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1144	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1145	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1146	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1146	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1146	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1147	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1147	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1147	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1149	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1149	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1150	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1150	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1150	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1151	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1151	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1154	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1154	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1154	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1156	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1156	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1156	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1157	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1157	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1158	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1158	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1158	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1159	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1160	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1160	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1160	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1161	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1161	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1162	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1162	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1163	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1164	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1166	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1166	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1167	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1167	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1169	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1172	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1172	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1173	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1174	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1174	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1175	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1175	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1176	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1177	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1177	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1177	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1178	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1178	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1178	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1180	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1180	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1181	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1183	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1183	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1183	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1184	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1184	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1185	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1187	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1187	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1188	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1188	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1188	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1189	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1190	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1190	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1191	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1191	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1192	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1192	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1192	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1193	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1194	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1194	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1195	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1195	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1196	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1196	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1204	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1204	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1209	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1209	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1220	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1220	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1303	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1303	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1303	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1304	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1304	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1304	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1305	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1305	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1306	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1306	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1306	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1307	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1307	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1309	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1309	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-13100	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-13100	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-13101	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-13101	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-13101	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1310	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1310	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1310	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1311	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1311	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1311	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1312	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1312	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1312	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1313	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1313	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1313	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1314	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1314	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1318	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1318	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1321	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1321	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1321	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1324	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1326	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1330	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1330	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1330	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1332	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1332	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1332	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1333	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1333	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1337	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1338	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1338	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1342	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1342	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1342	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1343	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1343	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1344	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1344	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1344	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1346	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1346	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1348	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1348	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1348	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1349	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1349	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1349	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1350	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1350	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1354	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1355	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1355	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1355	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1356	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1356	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1358	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1358	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1358	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1360	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1360	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1362	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1362	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1365	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1365	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1366	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1366	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1366	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1375	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1375	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1375	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1377	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1377	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1381	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1381	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1381	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1382	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1382	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1386	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1388	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1388	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1388	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1389	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1389	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1389	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1390	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1390	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1390	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1393	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1393	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1393	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1394	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1394	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1394	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1395	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1395	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1395	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1402	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1403	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1406	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1406	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1409	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1409	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1411	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1412	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1412	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1413	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1413	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1413	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1415	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1415	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1416	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1417	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1417	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1417	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1420	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1420	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1421	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1421	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1422	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1422	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1422	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1425	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1425	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1425	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1429	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1429	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1430	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1430	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1431	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1431	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1432	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1432	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1433	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1433	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1433	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1434	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1434	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1437	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1437	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1440	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1441	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1449	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1449	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1452	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1452	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1453	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1453	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1453	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1455	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1462	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1463	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1463	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1463	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1465	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1465	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1465	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1469	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1469	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1469	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1470	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1473	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1473	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1473	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1474	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1474	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1474	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1477	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1478	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1478	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1478	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1480	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1480	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1481	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1483	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1483	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1484	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1484	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1485	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1485	TDSQLVLLNKEKESN	0	15
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1485	SQLVLLNKEKESNGS	2	17
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1489	DSQLVLLNKEKESNG	1	16
RASAL2-KIF14	chr1	178252826	chr1	200544819	682	DRB1-1494	DSQLVLLNKEKESNG	1	16

Top

Fusion breakpoint peptide structures of RASAL2-KIF14

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

Top

Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of RASAL2-KIF14

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele

PDB ID

File name

BPseq

Docking score

Glide score

Top

Vaccine Design for the FusionNeoAntigens of RASAL2-KIF14

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene

Hchr

Hbp

Tchr

Tbp

Start in +/-13AA

End in +/-13AA

FusionNeoAntigen peptide sequence

FusionNeoAntigen RNA sequence

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
RASAL2-KIF14	chr1	178252826	chr1	200544819	0	15	TDSQLVLLNKEKESN	ACAGACAGCCAGTTGGTATTGCTCAACAAGGAGAAGGAGAGTAAT
RASAL2-KIF14	chr1	178252826	chr1	200544819	1	16	DSQLVLLNKEKESNG	GACAGCCAGTTGGTATTGCTCAACAAGGAGAAGGAGAGTAATGGT
RASAL2-KIF14	chr1	178252826	chr1	200544819	2	17	SQLVLLNKEKESNGS	AGCCAGTTGGTATTGCTCAACAAGGAGAAGGAGAGTAATGGTAGT

Top

Information of the samples that have these potential fusion neoantigens of RASAL2-KIF14

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type

Fusion gene

Hchr

Hbp

Henst

Tchr

Tbp

Tenst

Sample

Top

Potential target of CAR-T therapy development for RASAL2-KIF14

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

Top

Related Drugs to RASAL2-KIF14

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to RASAL2-KIF14

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)