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Center for Computational Systems Medicine
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Fusion Gene and Fusion Protein Summary

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Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)

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Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)

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Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)

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Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)

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Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)

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Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)

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Potential target of CAR-T therapy development

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Information on the samples that have these potential fusion neoantigens

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Fusion Protein Targeting Drugs - (Manual Curation)

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Fusion Protein Related diseases - (Manual Curation)

Fusion Protein:SLC30A4-AMACR

Fusion Gene and Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: SLC30A4-AMACR
FusionPDB ID: 82873
FusionGDB2.0 ID: 82873
HgeneTgene
Gene symbol

SLC30A4

AMACR

Gene ID

7782

23600

Gene namesolute carrier family 30 member 4alpha-methylacyl-CoA racemase
SynonymsZNT4|znT-4AMACRD|CBAS4|P504S|RACE|RM
Cytomap

15q21.1|15q21.1

5p13.2

Type of geneprotein-codingprotein-coding
Descriptionzinc transporter 4solute carrier family 30 (zinc transporter), member 4alpha-methylacyl-CoA racemase2-methylacyl-CoA racemase
Modification date2020031320200313
UniProtAcc.

Q9UHK6

Main function of 5'-partner protein: FUNCTION: Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359). {ECO:0000269|PubMed:10655068, ECO:0000269|PubMed:11060359, ECO:0000269|PubMed:7649182}.
Ensembl transtripts involved in fusion geneENST idsENST00000261867, ENST00000559667, 
ENST00000382068, ENST00000382085, 
ENST00000426255, ENST00000441713, 
ENST00000502637, ENST00000512079, 
ENST00000514195, ENST00000335606, 
ENST00000382072, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score8 X 3 X 1=2428 X 3 X 4=336
# samples 923
** MAII scorelog2(9/24*10)=1.90689059560852
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
log2(23/336*10)=-0.546827371834385
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Fusion gene context

PubMed: SLC30A4 [Title/Abstract] AND AMACR [Title/Abstract] AND fusion [Title/Abstract]

Fusion neoantigen context

PubMed: SLC30A4 [Title/Abstract] AND AMACR [Title/Abstract] AND neoantigen [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)SLC30A4(45814161)-AMACR(34006004), # samples:2
Anticipated loss of major functional domain due to fusion event.SLC30A4-AMACR seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC30A4-AMACR seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC30A4-AMACR seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
SLC30A4-AMACR seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneSLC30A4

GO:0009636

response to toxic substance

17575980

HgeneSLC30A4

GO:0061088

regulation of sequestering of zinc ion

17349999

TgeneAMACR

GO:0008206

bile acid metabolic process

10655068



check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr15:45814161/chr5:34006004)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonRetention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

check buttonFusion gene breakpoints across SLC30A4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across AMACR (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Amino Acid Sequences


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)
BP loci
(transcript)
Predicted start
(transcript)
Predicted stop
(transcript)
Seq length
(amino acids)
ENST00000261867SLC30A4chr1545814161-ENST00000335606AMACRchr534006004-45237063151607430
ENST00000261867SLC30A4chr1545814161-ENST00000382072AMACRchr534006004-33667063151055246

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000261867ENST00000335606SLC30A4chr1545814161-AMACRchr534006004-0.0002602430.9997397
ENST00000261867ENST00000382072SLC30A4chr1545814161-AMACRchr534006004-0.0020770240.99792296

check button Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for SLC30A4-AMACR

check button +/-13 AA sequence from the breakpoints of the fusion protein sequences.
HgeneHchrHbpTgeneTchrTbpLength(fusion protein)BP in fusion proteinPeptide
SLC30A4chr1545814161AMACRchr534006004706130AVLYLLFMIGELVGVMEKLQLGPEIL

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Potential FusionNeoAntigen Information of SLC30A4-AMACR in HLA I

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
SLC30A4-AMACR_45814161_34006004.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)
Fusion geneHchrHbpTgeneTchrTbpHLA IFusionNeoAntigen peptideBinding scoreImmunogenic scoreNeoantigen start (at BP 13)Neoantigen end (at BP 13)
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:22FMIGELVGV0.99940.836615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:11FMIGELVGV0.99930.7819615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:67FMIGELVGV0.99930.7313615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:24FMIGELVGV0.99930.7313615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:60FMIGELVGV0.99930.7521615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:30FMIGELVGV0.99930.7313615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:21FMIGELVGV0.99910.8231615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:16FMIGELVGV0.9990.6968615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:27FMIGELVGV0.99890.8285615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:13FMIGELVGV0.99850.8114615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:38FMIGELVGV0.99760.7403615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:19FMIGELVGV0.99730.6932615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:29FMIGELVGV0.99710.7377615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:35FMIGELVGV0.99660.7586615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:20FMIGELVGV0.99550.743615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:04FMIGELVGV0.99520.9195615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:17FMIGELVGV0.98980.8201615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:22LLFMIGELVGV0.9990.5706415
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:04LLFMIGELVGV0.9890.7894415
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:02FMIGELVGV0.99940.8394615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:07FMIGELVGV0.99930.7794615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:05FMIGELVGV0.99930.9095615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:01FMIGELVGV0.99930.7313615
SLC30A4-AMACRchr1545814161chr534006004706HLA-B44:10GELVGVMEKL0.91590.6187919
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:03FMIGELVGV0.99950.8858615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:06FMIGELVGV0.99910.8231615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A02:14FMIGELVGV0.99910.8082615
SLC30A4-AMACRchr1545814161chr534006004706HLA-A25:01ELVGVMEKL0.99450.91881019
SLC30A4-AMACRchr1545814161chr534006004706HLA-A68:02ELVGVMEKL0.98670.56931019
SLC30A4-AMACRchr1545814161chr534006004706HLA-A69:01ELVGVMEKL0.95890.6681019
SLC30A4-AMACRchr1545814161chr534006004706HLA-B40:04GELVGVMEKL0.98570.7915919

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Potential FusionNeoAntigen Information of SLC30A4-AMACR in HLA II

check button Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.
SLC30A4-AMACR_45814161_34006004.msa

check button Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).
Fusion geneHchrHbpTgeneTchrTbpHLA IIFusionNeoAntigen peptideNeoantigen start (at BP 13)Neoantigen end (at BP 13)
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1113GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1377GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1377IGELVGVMEKLQLGP823
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1404GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1411GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1428GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1431GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1431IGELVGVMEKLQLGP823
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1432GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1432IGELVGVMEKLQLGP823
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1434GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1435GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1455GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1461GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1465GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1465IGELVGVMEKLQLGP823
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1471GELVGVMEKLQLGPE924
SLC30A4-AMACRchr1545814161chr534006004706DRB1-1481GELVGVMEKLQLGPE924

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Fusion breakpoint peptide structures of SLC30A4-AMACR

check button3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.
File nameBPseqHgeneTgeneHchrHbpTchrTbpAAlen
2470FMIGELVGVMEKLQSLC30A4AMACRchr1545814161chr534006004706

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of SLC30A4-AMACR

check buttonVirtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.
HLA allelePDB IDFile nameBPseqDocking scoreGlide score
HLA-B14:023BVN2470FMIGELVGVMEKLQ-7.9962-8.1096
HLA-B14:023BVN2470FMIGELVGVMEKLQ-5.70842-6.74372
HLA-B52:013W392470FMIGELVGVMEKLQ-6.83737-6.95077
HLA-B52:013W392470FMIGELVGVMEKLQ-4.4836-5.5189
HLA-A11:014UQ22470FMIGELVGVMEKLQ-10.0067-10.1201
HLA-A11:014UQ22470FMIGELVGVMEKLQ-9.03915-10.0745
HLA-A24:025HGA2470FMIGELVGVMEKLQ-6.56204-6.67544
HLA-A24:025HGA2470FMIGELVGVMEKLQ-5.42271-6.45801
HLA-B44:053DX82470FMIGELVGVMEKLQ-7.85648-8.89178
HLA-B44:053DX82470FMIGELVGVMEKLQ-5.3978-5.5112
HLA-A02:016TDR2470FMIGELVGVMEKLQ-3.37154-4.40684

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Vaccine Design for the FusionNeoAntigens of SLC30A4-AMACR

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptide sequenceFusionNeoAntigen RNA sequence
SLC30A4-AMACRchr1545814161chr5340060041019ELVGVMEKLAACTTGTAGGTGTCATGGAGAAACTCC
SLC30A4-AMACRchr1545814161chr534006004415LLFMIGELVGVTGCTTTTCATGATTGGAGAACTTGTAGGTGTCA
SLC30A4-AMACRchr1545814161chr534006004615FMIGELVGVTCATGATTGGAGAACTTGTAGGTGTCA
SLC30A4-AMACRchr1545814161chr534006004919GELVGVMEKLGAGAACTTGTAGGTGTCATGGAGAAACTCC

check button mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.
Fusion geneHchrHbpTchrTbpStart in +/-13AAEnd in +/-13AAFusionNeoAntigen peptideFusionNEoAntigen RNA sequence
SLC30A4-AMACRchr1545814161chr534006004823IGELVGVMEKLQLGPTTGGAGAACTTGTAGGTGTCATGGAGAAACTCCAGCTGGGCCCAG
SLC30A4-AMACRchr1545814161chr534006004924GELVGVMEKLQLGPEGAGAACTTGTAGGTGTCATGGAGAAACTCCAGCTGGGCCCAGAGA

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Information of the samples that have these potential fusion neoantigens of SLC30A4-AMACR

check button These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.
Cancer typeFusion geneHchrHbpHenstTchrTbpTenstSample
PRADSLC30A4-AMACRchr1545814161ENST00000261867chr534006004ENST00000335606TCGA-YL-A8SC

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Potential target of CAR-T therapy development for SLC30A4-AMACR

check button Predicted 3D structure. We used RoseTTAFold.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features


* Minus value of BPloci means that the break point is located before the CDS.
- In-frame and retained 'Transmembrane'.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note

check button Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.
HgeneHchrHbpHenstTgeneTchrTbpTenstDeepLoc result

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Related Drugs to SLC30A4-AMACR

check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to SLC30A4-AMACR

check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource