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Fusion Protein:WAC-C10orf76

Fusion Gene and Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: WAC-C10orf76
	FusionPDB ID: 98668
	FusionGDB2.0 ID: 98668
		Hgene	Tgene
	Gene symbol	WAC	C10orf76
	Gene ID	51322	79591
	Gene name	WW domain containing adaptor with coiled-coil	armadillo like helical domain containing 3
	Synonyms	BM-016\|DESSH\|PRO1741\|Wwp4	C10orf76
	Cytomap	10p12.1\|10p12.1-p11.2	10q24.32
	Type of gene	protein-coding	protein-coding
	Description	WW domain-containing adapter protein with coiled-coil	armadillo-like helical domain-containing protein 3UPF0668 protein C10orf76
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000532233, ENST00000347934, ENST00000354911, ENST00000375646, ENST00000375664, ENST00000428935,	ENST00000370033, ENST00000311122, ENST00000495001,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	17 X 16 X 8=2176	8 X 6 X 5=240
	# samples	22	9
	** MAII score	log2(22/2176*10)=-3.30610312772568 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/240*10)=-1.41503749927884 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Fusion gene context	PubMed: WAC [Title/Abstract] AND C10orf76 [Title/Abstract] AND fusion [Title/Abstract]
Fusion neoantigen context	PubMed: WAC [Title/Abstract] AND C10orf76 [Title/Abstract] AND neoantigen [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	WAC(28824686)-C10orf76(103699695), # samples:1
Anticipated loss of major functional domain due to fusion event.	WAC-C10orf76 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. WAC-C10orf76 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:28824686/chr10:103699695)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Retention analysis results of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features, are available here.

Fusion gene breakpoints across WAC (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across C10orf76 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Amino Acid Sequences

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000375664	WAC	chr10	28824686	+	ENST00000370033	C10orf76	chr10	103699695	-	3042	748	609	1112	167
ENST00000375646	WAC	chr10	28824686	+	ENST00000370033	C10orf76	chr10	103699695	-	2751	457	318	821	167
ENST00000347934	WAC	chr10	28824686	+	ENST00000370033	C10orf76	chr10	103699695	-	2835	541	171	905	244
ENST00000354911	WAC	chr10	28824686	+	ENST00000370033	C10orf76	chr10	103699695	-	2729	435	65	799	244
ENST00000428935	WAC	chr10	28824686	+	ENST00000370033	C10orf76	chr10	103699695	-	2653	359	16	723	235

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000375664	ENST00000370033	WAC	chr10	28824686	+	C10orf76	chr10	103699695	-	0.008837459	0.99116254
ENST00000375646	ENST00000370033	WAC	chr10	28824686	+	C10orf76	chr10	103699695	-	0.006848722	0.9931513
ENST00000347934	ENST00000370033	WAC	chr10	28824686	+	C10orf76	chr10	103699695	-	0.007145078	0.9928549
ENST00000354911	ENST00000370033	WAC	chr10	28824686	+	C10orf76	chr10	103699695	-	0.009692678	0.99030733
ENST00000428935	ENST00000370033	WAC	chr10	28824686	+	C10orf76	chr10	103699695	-	0.009184924	0.99081504

Predicted full-length fusion amino acid sequences. For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among all the predicted ones.

Get the fusion protein sequences from here.

Fusion protein sequence information is available in the fasta format.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

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Fusion Protein Breakpoint Sequences for WAC-C10orf76

+/-13 AA sequence from the breakpoints of the fusion protein sequences.

Hgene	Hchr	Hbp	Tgene	Tchr	Tbp	Length(fusion protein)	BP in fusion protein	Peptide
WAC	chr10	28824686	C10orf76	chr10	103699695	359	114	KNVHTHRVRERDGVLRLSTNAGQWKE
WAC	chr10	28824686	C10orf76	chr10	103699695	435	123	KNVHTHRVRERDGVLRLSTNAGQWKE
WAC	chr10	28824686	C10orf76	chr10	103699695	457	46	KNVHTHRVRERDGVLRLSTNAGQWKE
WAC	chr10	28824686	C10orf76	chr10	103699695	541	123	KNVHTHRVRERDGVLRLSTNAGQWKE
WAC	chr10	28824686	C10orf76	chr10	103699695	748	46	KNVHTHRVRERDGVLRLSTNAGQWKE

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Potential FusionNeoAntigen Information of WAC-C10orf76 in HLA I

Multiple sequence alignments of the potential FusionNeoAntigens per fusion breakpoints. If the MSA is empty, then it means that there were predicted fusion neoantigens in this fusion breakpoint, but those predicted fusion neoantigens were not across the breakpoint, which is not fusion-specific.

WAC-C10orf76_28824686_103699695.msa

Potential FusionNeoAntigen Information
* We used NetMHCpan v4.1 (%rank<0.5) and deepHLApan v1.1 (immunogenic score>0.5)

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA I	FusionNeoAntigen peptide	Binding score	Immunogenic score	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:24	ERDGVLRL	0.9998	0.5214	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:01	ERDGVLRL	0.9995	0.7737	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B38:02	ERDGVLRL	0.9992	0.9378	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B48:01	RERDGVLRL	0.9986	0.5717	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B13:02	RERDGVLRL	0.9926	0.619	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B13:01	RERDGVLRL	0.9713	0.8963	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B47:01	RERDGVLRL	0.9443	0.5037	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B44:03	RERDGVLRL	0.9079	0.8914	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B50:02	RERDGVLRL	0.8637	0.5485	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B45:01	RERDGVLRL	0.7285	0.6759	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:13	RERDGVLRL	0.3893	0.7609	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B41:01	RERDGVLRL	0.366	0.8379	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:03	RERDGVLRL	0.1102	0.8439	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B50:01	RERDGVLRL	0.0353	0.6566	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B52:01	RERDGVLRL	0.0022	0.7704	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B27:04	VRERDGVLRL	0.9981	0.61	7	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B27:07	VRERDGVLRL	0.9973	0.5164	7	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A30:08	RVRERDGVLR	0.9961	0.6753	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A74:11	RVRERDGVLR	0.9913	0.5364	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A74:03	RVRERDGVLR	0.9913	0.5364	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A74:09	RVRERDGVLR	0.9913	0.5364	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A31:02	RVRERDGVLR	0.9827	0.5049	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A30:08	RVRERDGVLRL	0.9976	0.8784	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B48:01	RVRERDGVLRL	0.8753	0.7329	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:01	RVRERDGVLRL	0.4505	0.5584	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:09	ERDGVLRL	0.9997	0.6334	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:12	ERDGVLRL	0.9994	0.7896	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:05	ERDGVLRL	0.9989	0.754	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B14:03	ERDGVLRL	0.949	0.8206	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:06	RERDGVLRL	0.9989	0.6165	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:03	RERDGVLRL	0.9777	0.5516	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B07:12	RVRERDGVL	0.9771	0.6204	6	15
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:04	RVRERDGVL	0.782	0.8162	6	15
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B73:01	ERDGVLRLS	0.7581	0.6332	9	18
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:08	RERDGVLRL	0.5183	0.8375	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:04	RERDGVLRL	0.0593	0.9226	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B48:03	RVRERDGVLRL	0.338	0.6012	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:31	ERDGVLRL	0.9995	0.7956	9	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:04	RERDGVLRL	0.9983	0.6402	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:21	RERDGVLRL	0.9817	0.5453	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B44:07	RERDGVLRL	0.9079	0.8914	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B44:13	RERDGVLRL	0.9079	0.8914	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B44:26	RERDGVLRL	0.9079	0.8914	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:73	RVRERDGVL	0.776	0.774	6	15
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:30	RVRERDGVL	0.5769	0.7132	6	15
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B41:03	RERDGVLRL	0.5187	0.6566	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B39:02	RERDGVLRL	0.4793	0.7624	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B18:11	RERDGVLRL	0.4636	0.8805	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:54	RERDGVLRL	0.1853	0.8746	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:68	RERDGVLRL	0.1417	0.6087	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B48:02	RERDGVLRL	0.1354	0.9233	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:53	RERDGVLRL	0.0516	0.8871	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B15:73	RERDGVLRL	0.0502	0.8304	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B50:05	RERDGVLRL	0.0353	0.6566	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B50:04	RERDGVLRL	0.0353	0.6566	8	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B27:10	VRERDGVLRL	0.9979	0.6857	7	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B27:09	VRERDGVLRL	0.9965	0.538	7	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A30:01	RVRERDGVLR	0.9962	0.8109	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B27:06	VRERDGVLRL	0.9962	0.6236	7	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A74:01	RVRERDGVLR	0.9913	0.5364	6	16
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B48:05	RVRERDGVLRL	0.998	0.6318	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-A30:01	RVRERDGVLRL	0.9978	0.9445	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B41:03	RVRERDGVLRL	0.567	0.6788	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:49	RVRERDGVLRL	0.4338	0.5832	6	17
WAC-C10orf76	chr10	28824686	chr10	103699695	541	HLA-B40:12	RVRERDGVLRL	0.338	0.6012	6	17

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Potential FusionNeoAntigen Information of WAC-C10orf76 in HLA II

WAC-C10orf76_28824686_103699695.msa

Potential FusionNeoAntigen Information
* We used NetMHCIIpan v4.1 (%rank<0.5).

Fusion gene	Hchr	Hbp	Tgene	Tchr	Tbp	HLA II	FusionNeoAntigen peptide	Neoantigen start (at BP 13)	Neoantigen end (at BP 13)
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0401	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0403	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0403	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0403	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0403	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0407	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0407	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0413	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0413	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0413	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0413	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0415	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0415	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0415	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0415	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0419	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0419	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0422	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0427	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0427	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0427	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0427	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0431	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0433	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0435	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0435	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0436	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0436	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0436	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0436	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0437	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0437	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0437	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0438	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0438	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0439	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0439	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0439	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0439	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0440	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0440	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0440	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0440	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0441	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0441	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0441	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0441	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0442	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0442	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0442	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0442	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0443	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0444	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0444	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0444	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0444	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0446	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0446	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0446	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0446	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0447	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0449	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0449	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0449	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0449	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0450	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0450	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0450	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0450	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0451	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0451	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0451	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0451	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0452	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0452	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0452	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0452	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0453	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0453	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0453	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0453	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0454	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0455	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0455	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0455	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0455	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0456	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0456	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0456	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0456	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0458	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0458	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0458	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0458	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0459	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0459	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0459	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0459	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0460	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0460	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0460	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0460	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0461	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0461	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0463	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0465	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0465	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0465	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0468	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0468	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0468	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0468	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0470	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0470	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0470	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0470	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0471	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0471	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0471	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0471	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0473	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0473	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0473	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0473	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0474	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0474	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0475	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0475	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0476	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0478	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0478	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0478	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0479	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0479	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0479	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0479	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0485	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0485	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0485	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0485	RERDGVLRLSTNAGQ	8	23
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0488	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0488	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-0488	ERDGVLRLSTNAGQW	9	24
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-1002	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-1410	DGVLRLSTNAGQWKE	11	26
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-1410	RDGVLRLSTNAGQWK	10	25
WAC-C10orf76	chr10	28824686	chr10	103699695	541	DRB1-1457	RDGVLRLSTNAGQWK	10	25

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Fusion breakpoint peptide structures of WAC-C10orf76

3D structures of the fusion breakpoint peptide of 14AA sequence that have potential fusion neoantigens
* The minimum length of the amino acid sequence in RoseTTAFold is 14AA. Here, we predicted the 14AA fusion protein breakpoint sequence not the fusion neoantigen peptide, which is shorter than 14 AA.

File name	BPseq	Hgene	Tgene	Hchr	Hbp	Tchr	Tbp	AAlen
8328	RVRERDGVLRLSTN	WAC	C10orf76	chr10	28824686	chr10	103699695	541

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Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D of WAC-C10orf76

Virtual screening between 25 HLAs (from PDB) and FusionNeoAntigens
* We used Glide to predict the interaction between HLAs and neoantigens.

HLA allele	PDB ID	File name	BPseq	Docking score	Glide score
HLA-B14:02	3BVN	8328	RVRERDGVLRLSTN	-8.62545	-8.73885
HLA-B14:02	3BVN	8328	RVRERDGVLRLSTN	-3.26321	-4.29851
HLA-B52:01	3W39	8328	RVRERDGVLRLSTN	-6.23413	-6.34753
HLA-B52:01	3W39	8328	RVRERDGVLRLSTN	-4.55402	-5.58932
HLA-A24:02	5HGA	8328	RVRERDGVLRLSTN	-8.62578	-8.73918
HLA-A24:02	5HGA	8328	RVRERDGVLRLSTN	-6.438	-7.4733
HLA-B44:05	3DX8	8328	RVRERDGVLRLSTN	-5.68484	-5.79824
HLA-B44:05	3DX8	8328	RVRERDGVLRLSTN	-3.64855	-4.68385
HLA-A02:01	6TDR	8328	RVRERDGVLRLSTN	-5.14764	-6.18294

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Vaccine Design for the FusionNeoAntigens of WAC-C10orf76

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-Is.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide sequence	FusionNeoAntigen RNA sequence
WAC-C10orf76	chr10	28824686	chr10	103699695	6	15	RVRERDGVL	GAGTTAGAGAGAGGGATGGTGTCCTGA
WAC-C10orf76	chr10	28824686	chr10	103699695	6	16	RVRERDGVLR	GAGTTAGAGAGAGGGATGGTGTCCTGAGGC
WAC-C10orf76	chr10	28824686	chr10	103699695	6	17	RVRERDGVLRL	GAGTTAGAGAGAGGGATGGTGTCCTGAGGCTTT
WAC-C10orf76	chr10	28824686	chr10	103699695	7	17	VRERDGVLRL	TTAGAGAGAGGGATGGTGTCCTGAGGCTTT
WAC-C10orf76	chr10	28824686	chr10	103699695	8	17	RERDGVLRL	GAGAGAGGGATGGTGTCCTGAGGCTTT
WAC-C10orf76	chr10	28824686	chr10	103699695	9	17	ERDGVLRL	AGAGGGATGGTGTCCTGAGGCTTT
WAC-C10orf76	chr10	28824686	chr10	103699695	9	18	ERDGVLRLS	AGAGGGATGGTGTCCTGAGGCTTTCTA

mRNA and peptide sequences of FusionNeoAntigens that have potential interaction with HLA-IIs.

Fusion gene	Hchr	Hbp	Tchr	Tbp	Start in +/-13AA	End in +/-13AA	FusionNeoAntigen peptide	FusionNEoAntigen RNA sequence
WAC-C10orf76	chr10	28824686	chr10	103699695	10	25	RDGVLRLSTNAGQWK	GGGATGGTGTCCTGAGGCTTTCTACCAATGCAGGCCAGTGGAAGG
WAC-C10orf76	chr10	28824686	chr10	103699695	11	26	DGVLRLSTNAGQWKE	ATGGTGTCCTGAGGCTTTCTACCAATGCAGGCCAGTGGAAGGAAG
WAC-C10orf76	chr10	28824686	chr10	103699695	8	23	RERDGVLRLSTNAGQ	GAGAGAGGGATGGTGTCCTGAGGCTTTCTACCAATGCAGGCCAGT
WAC-C10orf76	chr10	28824686	chr10	103699695	9	24	ERDGVLRLSTNAGQW	AGAGGGATGGTGTCCTGAGGCTTTCTACCAATGCAGGCCAGTGGA

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Information of the samples that have these potential fusion neoantigens of WAC-C10orf76

These samples were reported as having these fusion breakpoints. For individual breakpoints, we checked the open reading frames considering multiple gene isoforms and chose the in-frame fusion genes only. Then, we made fusion protein sequences and predicted the fusion neoantigens. These fusion-positive samples may have these potential fusion neoantigens.

Cancer type	Fusion gene	Hchr	Hbp	Henst	Tchr	Tbp	Tenst	Sample
READ	WAC-C10orf76	chr10	28824686	ENST00000347934	chr10	103699695	ENST00000370033	TCGA-AG-3887

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Potential target of CAR-T therapy development for WAC-C10orf76

Predicted 3D structure. We used RoseTTAFold.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, to provide the retention of the transmembrane domain, we only show the protein feature retention information of those transmembrane features

* Minus value of BPloci means that the break point is located before the CDS.

- In-frame and retained 'Transmembrane'.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Subcellular localization prediction of the transmembrane domain retained fusion proteins
* We used DeepLoc 1.0. The order of the X-axis of the barplot is as follows: Entry_ID, Localization, Type, Nucleus, Cytoplasm, Extracellular, Mitochondrion, Cell_membrane, Endoplasmic_reticulum, Plastid, Golgi.apparatus, Lysosome.Vacuole, Peroxisome. Y-axis is the output score of DeepLoc. Clicking the image will open a new tab with a large image.

Hgene

Hchr

Hbp

Henst

Tgene

Tchr

Tbp

Tenst

DeepLoc result

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Related Drugs to WAC-C10orf76

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to WAC-C10orf76

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source

	Fusion Gene and Fusion Protein Summary
	Fusion Amino Acid Sequences (multiple BPs and multiple gene isoforms)
	Fusion Protein Breakpoint Sequences - (for the Screening of the FusionNeoAntigens)
	Potential FusionNeoAntigens in HLA I - (netMHCpan v4.1 + deepHLApan v1.1)
	Potential FusionNeoAntigens in HLA II - (netMHCIIpan v4.1)
	Fusion Breakpoint 14 AA Peptide Structure - (RoseTTAFold)
	Filtering FusionNeoAntigens Through Checking the Interaction with HLAs in 3D - (Glide)
	Vaccine Design for the FusionNeoAntigens (RNA/protein sequences)
	Potential target of CAR-T therapy development
	Information on the samples that have these potential fusion neoantigens
	Fusion Protein Targeting Drugs - (Manual Curation)
	Fusion Protein Related diseases - (Manual Curation)