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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Potential Active Site Information
	Potentially Interacting Small Molecules through Virtual Screening
	Biochemical Features of Small Molecules with ADME
	Drug Toxicity Information
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:YWHAE-NUTM2A

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: YWHAE-NUTM2A
	FusionPDB ID: 100147
	FusionGDB2.0 ID: 100147
		Hgene	Tgene
	Gene symbol	YWHAE	NUTM2A
	Gene ID	7531	728118
	Gene name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon	NUT family member 2A
	Synonyms	14-3-3E\|HEL2\|KCIP-1\|MDCR\|MDS	FAM22A
	Cytomap	17p13.3	10q23.2
	Type of gene	protein-coding	protein-coding
	Description	14-3-3 protein epsilon14-3-3 epsilonepididymis luminal protein 2mitochondrial import stimulation factor L subunitprotein kinase C inhibitor protein-1tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptidetyrosine	NUT family member 2Afamily with sequence similarity 22, member Aprotein FAM22A
	Modification date	20200327	20200313
	UniProtAcc	P62258	Q8IVF1
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000571732, ENST00000264335, ENST00000498643, ENST00000573026, ENST00000575977,	ENST00000381689, ENST00000381707,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	28 X 9 X 15=3780	1 X 1 X 2=2
	# samples	42	2
	** MAII score	log2(42/3780*10)=-3.16992500144231 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(2/2*10)=3.32192809488736
Context (manual curation of fusion genes in FusionPDB)	PubMed: YWHAE [Title/Abstract] AND NUTM2A [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	YWHAE(1257505)-NUTM2A(88988019), # samples:1
Anticipated loss of major functional domain due to fusion event.	YWHAE-NUTM2A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. YWHAE-NUTM2A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. YWHAE-NUTM2A seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. YWHAE-NUTM2A seems lost the major protein functional domain in Hgene partner, which is a epigenetic factor due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	YWHAE	GO:0000165	MAPK cascade	12917326
Hgene	YWHAE	GO:0034605	cellular response to heat	12917326
Hgene	YWHAE	GO:0046827	positive regulation of protein export from nucleus	12917326
Hgene	YWHAE	GO:0051480	regulation of cytosolic calcium ion concentration	18029012
Hgene	YWHAE	GO:0060306	regulation of membrane repolarization	11953308
Hgene	YWHAE	GO:1901016	regulation of potassium ion transmembrane transporter activity	11953308
Hgene	YWHAE	GO:1901020	negative regulation of calcium ion transmembrane transporter activity	18029012
Hgene	YWHAE	GO:1902309	negative regulation of peptidyl-serine dephosphorylation	11953308
Hgene	YWHAE	GO:1905913	negative regulation of calcium ion export across plasma membrane	18029012

Fusion gene breakpoints across YWHAE (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across NUTM2A (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerKB3	.	.	YWHAE	chr17	1257504	-	NUTM2A	chr10	88988019	+
ChiTaRS5.0	N/A	JN999698	YWHAE	chr17	1257505	-	NUTM2A	chr10	88988019	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000264335	YWHAE	chr17	1257504	-	ENST00000381707	NUTM2A	chr10	88988019	+	3508	983	202	3237	1011
ENST00000264335	YWHAE	chr17	1257504	-	ENST00000381689	NUTM2A	chr10	88988019	+	4244	983	202	2490	762
ENST00000264335	YWHAE	chr17	1257505	-	ENST00000381707	NUTM2A	chr10	88988019	+	3508	983	202	3237	1011
ENST00000264335	YWHAE	chr17	1257505	-	ENST00000381689	NUTM2A	chr10	88988019	+	4244	983	202	2490	762

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000264335	ENST00000381707	YWHAE	chr17	1257505	-	NUTM2A	chr10	88988019	+	0.002160282	0.9978397
ENST00000264335	ENST00000381689	YWHAE	chr17	1257505	-	NUTM2A	chr10	88988019	+	0.004238803	0.9957612

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>100147_100147_1_YWHAE-NUTM2A_YWHAE_chr17_1257504_ENST00000264335_NUTM2A_chr10_88988019_ENST00000381689_length(amino acids)=762AA_BP=260
MRESETLSASIRRADPAGAAAAMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQ
KEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDI
AMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDAYPALGPGVT
ANPGTSLSVFTALPFTTPAPGPAHGPLLVTAGAPPGGPLVLSTLPSTPLVTEQDGCGPSGAGASNVFVQMRTEVGPVKAAQAQTLVLTQA
PLVWQAPGALCGGVVCPPPLLLAAAPVVPVMAAQVVGGTQACEGGWSQGLPLPPPPPPAAQLPPIVSQGNAGPWPQGAHGEGSLASSQAK
APPDDSCNPRSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSLARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLE
FEAEEEMQIQKSQWMKGPQCLPPPATPRLEPRGPPAPEVVKQPVYLPSKAGPKAPTACLPPPRPQRPVTKARRPPPRPHRRAETKARLPP
PRPQRPAETKVPEEIPPEVVQEYVDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYTDKLCSQKDFVTKVEAVIHPQF

--------------------------------------------------------------

>100147_100147_2_YWHAE-NUTM2A_YWHAE_chr17_1257504_ENST00000264335_NUTM2A_chr10_88988019_ENST00000381707_length(amino acids)=1011AA_BP=260
MRESETLSASIRRADPAGAAAAMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQ
KEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDI
AMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDAYPALGPGVT
ANPGTSLSVFTALPFTTPAPGPAHGPLLVTAGAPPGGPLVLSTLPSTPLVTEQDGCGPSGAGASNVFVQMRTEVGPVKAAQAQTLVLTQA
PLVWQAPGALCGGVVCPPPLLLAAAPVVPVMAAQVVGGTQACEGGWSQGLPLPPPPPPAAQLPPIVSQGNAGPWPQGAHGEGSLASSQAK
APPDDSCNPRSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSLARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLE
FEAEEEMQIQKSQWMKGPQCLPPPATPRLEPRGPPAPEVVKQPVYLPSKAGPKAPTACLPPPRPQRPVTKARRPPPRPHRRAETKARLPP
PRPQRPAETKVPEEIPPEVVQEYVDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYTDKLCSQKDFVTKVEAVIHPQF
LEELLSPDPQMDFLALSQELEQEEGLTLAQLVEKRLLPLKEKQHARAAPSRGTARLDSSSSKFAAGQGAERDVPVPQQGVGMETCPPQTT
ARDSQGRGRAHTGMARSKDSVVLLGCQDSPGLRAARPTSPPQDHRPTCPGVGTKDALDLPGGSPVRESHGLAQGSSEEEELPSLAFLLGS
QHKLLPWWLPQSPVPASGLLSPEKWGPQGTHQFPSAERRGLNLAPSPANKAKKRPLFGSLSPAEKTPHPGPGLRVSGEQSLTWGLGGPSQ

--------------------------------------------------------------

>100147_100147_3_YWHAE-NUTM2A_YWHAE_chr17_1257505_ENST00000264335_NUTM2A_chr10_88988019_ENST00000381689_length(amino acids)=762AA_BP=260
MRESETLSASIRRADPAGAAAAMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQ
KEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDI
AMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDAYPALGPGVT
ANPGTSLSVFTALPFTTPAPGPAHGPLLVTAGAPPGGPLVLSTLPSTPLVTEQDGCGPSGAGASNVFVQMRTEVGPVKAAQAQTLVLTQA
PLVWQAPGALCGGVVCPPPLLLAAAPVVPVMAAQVVGGTQACEGGWSQGLPLPPPPPPAAQLPPIVSQGNAGPWPQGAHGEGSLASSQAK
APPDDSCNPRSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSLARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLE
FEAEEEMQIQKSQWMKGPQCLPPPATPRLEPRGPPAPEVVKQPVYLPSKAGPKAPTACLPPPRPQRPVTKARRPPPRPHRRAETKARLPP
PRPQRPAETKVPEEIPPEVVQEYVDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYTDKLCSQKDFVTKVEAVIHPQF

--------------------------------------------------------------

>100147_100147_4_YWHAE-NUTM2A_YWHAE_chr17_1257505_ENST00000264335_NUTM2A_chr10_88988019_ENST00000381707_length(amino acids)=1011AA_BP=260
MRESETLSASIRRADPAGAAAAMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQ
KEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDI
AMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDAYPALGPGVT
ANPGTSLSVFTALPFTTPAPGPAHGPLLVTAGAPPGGPLVLSTLPSTPLVTEQDGCGPSGAGASNVFVQMRTEVGPVKAAQAQTLVLTQA
PLVWQAPGALCGGVVCPPPLLLAAAPVVPVMAAQVVGGTQACEGGWSQGLPLPPPPPPAAQLPPIVSQGNAGPWPQGAHGEGSLASSQAK
APPDDSCNPRSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSLARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLE
FEAEEEMQIQKSQWMKGPQCLPPPATPRLEPRGPPAPEVVKQPVYLPSKAGPKAPTACLPPPRPQRPVTKARRPPPRPHRRAETKARLPP
PRPQRPAETKVPEEIPPEVVQEYVDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYTDKLCSQKDFVTKVEAVIHPQF
LEELLSPDPQMDFLALSQELEQEEGLTLAQLVEKRLLPLKEKQHARAAPSRGTARLDSSSSKFAAGQGAERDVPVPQQGVGMETCPPQTT
ARDSQGRGRAHTGMARSKDSVVLLGCQDSPGLRAARPTSPPQDHRPTCPGVGTKDALDLPGGSPVRESHGLAQGSSEEEELPSLAFLLGS
QHKLLPWWLPQSPVPASGLLSPEKWGPQGTHQFPSAERRGLNLAPSPANKAKKRPLFGSLSPAEKTPHPGPGLRVSGEQSLTWGLGGPSQ

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:1257505/chr10:88988019)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
YWHAE P62258	NUTM2A Q8IVF1
FUNCTION: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed:12917326). {ECO:0000250\|UniProtKB:P62261, ECO:0000269\|PubMed:12917326}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

NUTM2A

chr17:1257505

chr10:88988019

ENST00000381689

278_285

127.33333333333333

630.0

Compositional bias

Note=Poly-Pro

Tgene

NUTM2A

chr17:1257505

chr10:88988019

ENST00000381689

425_514

127.33333333333333

630.0

Compositional bias

Note=Pro-rich

Tgene

NUTM2A

chr17:1257505

chr10:88988019

ENST00000381707

278_285

127.33333333333333

879.0

Compositional bias

Note=Poly-Pro

Tgene

NUTM2A

chr17:1257505

chr10:88988019

ENST00000381707

425_514

127.33333333333333

879.0

Compositional bias

Note=Pro-rich

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)

PDB file (622) >>>622.pdbFusion protein BP residue: 260 CIF file (622) >>>622.cif	YWHAE	chr17	1257504	-	NUTM2A	chr10	88988019	+	MRESETLSASIRRADPAGAAAAMDDREDLVYQAKLAEQAERYDEMVESMK KVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDK LKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDY HRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS VFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNL TLWTSDMQGDAYPALGPGVTANPGTSLSVFTALPFTTPAPGPAHGPLLVT AGAPPGGPLVLSTLPSTPLVTEQDGCGPSGAGASNVFVQMRTEVGPVKAA QAQTLVLTQAPLVWQAPGALCGGVVCPPPLLLAAAPVVPVMAAQVVGGTQ ACEGGWSQGLPLPPPPPPAAQLPPIVSQGNAGPWPQGAHGEGSLASSQAK APPDDSCNPRSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSL ARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLEFEAEEEMQIQ KSQWMKGPQCLPPPATPRLEPRGPPAPEVVKQPVYLPSKAGPKAPTACLP PPRPQRPVTKARRPPPRPHRRAETKARLPPPRPQRPAETKVPEEIPPEVV QEYVDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYTD KLCSQKDFVTKVEAVIHPQFLEELLSPDPQMDFLALSQELEQEEGLTLAQ	762
3D view using mol* of 622 (AA BP:260)


PDB file (756) >>>756.pdbFusion protein BP residue: 260 CIF file (756) >>>756.cif	YWHAE	chr17	1257504	-	NUTM2A	chr10	88988019	+	MRESETLSASIRRADPAGAAAAMDDREDLVYQAKLAEQAERYDEMVESMK KVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDK LKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDY HRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFS VFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNL TLWTSDMQGDAYPALGPGVTANPGTSLSVFTALPFTTPAPGPAHGPLLVT AGAPPGGPLVLSTLPSTPLVTEQDGCGPSGAGASNVFVQMRTEVGPVKAA QAQTLVLTQAPLVWQAPGALCGGVVCPPPLLLAAAPVVPVMAAQVVGGTQ ACEGGWSQGLPLPPPPPPAAQLPPIVSQGNAGPWPQGAHGEGSLASSQAK APPDDSCNPRSVYENFRLWQHYKPLARRHLPQSPDTEALSCFLIPVLRSL ARRKPTMTLEEGLWRAMREWQHTSNFDRMIFYEMAEKFLEFEAEEEMQIQ KSQWMKGPQCLPPPATPRLEPRGPPAPEVVKQPVYLPSKAGPKAPTACLP PPRPQRPVTKARRPPPRPHRRAETKARLPPPRPQRPAETKVPEEIPPEVV QEYVDIMEELLGPSLGATGEPEKQREEGEVKQPQEEDWTPPDPGLLSYTD KLCSQKDFVTKVEAVIHPQFLEELLSPDPQMDFLALSQELEQEEGLTLAQ LVEKRLLPLKEKQHARAAPSRGTARLDSSSSKFAAGQGAERDVPVPQQGV GMETCPPQTTARDSQGRGRAHTGMARSKDSVVLLGCQDSPGLRAARPTSP PQDHRPTCPGVGTKDALDLPGGSPVRESHGLAQGSSEEEELPSLAFLLGS QHKLLPWWLPQSPVPASGLLSPEKWGPQGTHQFPSAERRGLNLAPSPANK AKKRPLFGSLSPAEKTPHPGPGLRVSGEQSLTWGLGGPSQSQKRKGDPLV	1011
3D view using mol* of 756 (AA BP:260)

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

YWHAE_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

YWHAE_NUTM2A_622_pLDDT.png (AA BP:260)

YWHAE_NUTM2A_622_pLDDT_and_active_sites.png (AA BP:260)

YWHAE_NUTM2A_622_violinplot.png (AA BP:260)

YWHAE_NUTM2A_756_pLDDT.png (AA BP:260)

YWHAE_NUTM2A_756_pLDDT_and_active_sites.png (AA BP:260)

YWHAE_NUTM2A_756_violinplot.png (AA BP:260)

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

YWHAE_NUTM2A_622.png

YWHAE_NUTM2A_756.png

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Potential Active Site Information

The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.

Fusion AA seq ID in FusionPDB	Site score	Size	D score	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
622	1.024	230	1.056	974.12	0.664	0.705	0.901	0.68	0.942	0.722	1.081	Chain A: 68,69,71,72,75,76,79,142,145,146,149,150, 153,191,194,197,198,241,242,245,284,285,286,287,28 8,355,356,357,358,359,360,361,362,363,364,462,465, 473,477,487,529,532,533,534,535,536,537,538,539,54 0,542,543,544,546,547
756	1.0446	234	1.1025	680.512	0.6034	0.6839	0.8388	0.8353	0.7603	1.0985	0.9203	Chain A: 37,38,453,454,455,456,457,458,459,461,462 ,464,465,494,495,498,502,526,527,529,530,531,533,5 34,537,538,541,897,898,900,901,902,903,904,905,906 ,907,908,909,910

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Potentially Interacting Small Molecules through Virtual Screening

The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.

Fusion AA seq ID in FusionPDB

ZINC ID

DrugBank ID

Drug name

Docking score

Glide gscore

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Drug information from DrugBank of the top 20 interacting small molecules.

ZINC ID

DrugBank ID

Drug name

Drug type

SMILES

Drug group

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Biochemical Features of Small Molecules

ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)

ZINC ID

mol_MW

dipole

SASA

FOSA

FISA

PISA

WPSA

volume

donorHB

accptHB

Human Oral Absorption

Percent Human Oral Absorption

Rule Of Five

Rule Of Three

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Drug Toxicity Information

Toxicity information of individual drugs using eToxPred

ZINC ID

Smile

Surface Accessibility

Toxicity

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

YWHAE

IGF1R, GPRIN2, SORBS2, GRAP2, MAPK7, NDEL1, HDAC4, HDAC5, REM1, BAD, MAP3K10, CDC25C, MAP3K3, MAP3K1, MAP3K2, IRS1, NGFRAP1, SYN2, KCNH2, TNFAIP3, RAF1, CDC25B, TOP2A, TGFB1, CDC25A, Usp8, USP43, POLR3H, HDAC7, MDM4, FOXO3, HIVEP2, LRMP, COX2, MYH10, PNLIP, SSFA2, YWHAB, YWHAZ, YWHAG, ARAF, YWHAH, HDAC9, KIAA0232, TLK1, CHAF1A, CAP2, YWHAQ, RAP1GAP2, SH3BP4, WWTR1, FAM13B, MSL2, ZNF839, RASAL3, WNK1, ENKD1, TBC1D3F, CGNL1, CEP95, ANKHD1-EIF4EBP3, ING1, SRRM2, FAN1, EMD, SAMSN1, EXO1, H2AFX, MYC, PRKAA1, PARD6G, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, DISC1, UBE3A, GAPDH, KAT8, HIST1H3A, HIST1H4A, TCEB3, BRD4, CALM1, FBXO4, CDKN1B, TRAT1, RARRES3, ARRB1, ARRB2, CBL, ACD, POT1, CUL3, CDK2, CUL1, DCUN1D1, NEDD8, PTPN14, MARK3, MARK2, MEX3B, LRRK2, AKT1, ACTA2, CBX3, HNF1A, HSP90AB1, NPM1, ATP5B, ATP5A1, STOML2, CLNS1A, IPO8, KPNB1, RPLP0, RPL6, RPL4, RPS3, RPS8, DNAJA1, EIF4B, EIF3E, EEF1G, EEF1A2, HNRNPA2B1, ILF2, RUVBL2, RCN2, TUBB, TUBB4B, TUBB2B, PRPSAP1, TUBA3E, DDX21, ADH1B, ADH4, AGXT, HIST2H4B, HNRNPA3, HNRNPC, RBMY1A1, SF3B1, PCBP1, RBM10, MAP3K7, TBK1, PPM1B, SPIN1, CDC37, PHB, PRPSAP2, PRPS1, QPCTL, TMPO, C11orf84, HDX, CFAP43, VCP, ATXN1, FN1, VCAM1, BRAF, MAP2K1, PARD3, KIF5B, KLC2, LMO7, TBC1D4, HSPA1A, KLC3, KLC4, KLC1, CLASP2, LIMA1, TSC2, CLASP1, HSPA8, PAK4, RAB11FIP2, MAST3, TBC1D1, KIF1C, OSBPL3, TIAM1, ABLIM1, LARP1, BAIAP2, IRS2, SHROOM2, LSR, KIF1B, RAB11FIP1, MLLT4, MAST2, PFKFB2, CDK18, PKP2, DENND4A, TP53BP2, CGN, ZFP36L2, TUBA1A, KSR1, RABEP1, EIF4E2, CSNK1A1, PDZD11, PRKCI, DCAF7, NADK, USP8, C1QBP, TSC1, REEP1, KIF23, SLC25A6, SRGAP2, FAM53C, TRIP11, BCAR1, CRTC1, RASSF8, CEP250, VAMP8, GRB2, TRIM32, NOS2, IL7R, UBL4A, ITGA4, gag-pol, CBX4, ABL1, MST1R, PAN2, RAD52, BAG3, UL46, GSTA1, FTH1, Wwtr1, Yap1, CDK11B, SRSF1, SRSF6, SRSF4, NOLC1, TRA2B, PLEKHO2, HMHA1, SRSF7, NCBP1, DOK3, VASP, KIAA0930, GAB2, KIAA0226, PIK3R4, SGK223, RCHY1, IGHG1, UVRAG, SRSF2, FBXO6, RASSF2, SAV1, YAP1, PARK2, PAFAH1B2, PPP1R2, PROSC, RAP1GDS1, TMOD3, TNFAIP8, TUBB2A, UBXN1, VCL, XPO1, ALDH7A1, ASNS, ATIC, CAPN2, CAPNS1, EIF5, FERMT2, G6PD, GSS, ISOC1, MCTS1, STK26, OGFOD1, PAK2, PDHB, PDIA4, PTMA, SCPEP1, TBCB, TWF2, UBE2R2, IRS4, SIK3, SIK2, CEP57, TP53, GRB10, DTL, HUWE1, FBXW11, Mdm4, CUL7, OBSL1, CCDC8, MAST1, CDK16, TNK1, BTRC, NKD2, DMTN, TEX33, Numb, EPB41L3, WWC1, HNRNPA1, HSPB1, UNK, ACAT2, CALR, CENPE, MACF1, MAPK14, MCFD2, POLR2D, PSMB2, SHMT2, SNRPD1, ACTR6, HNRNPL, NPM3, PGK1, PSMC1, ZPR1, NTRK1, LCA5, CENPJ, PRICKLE3, MYH11, TSNAX, CCDC88A, CRY1, CRY2, MCM2, Ksr1, CDC5L, ERRFI1, U2AF2, PRDX6, NFATC2, SNF8, ANKZF1, SMAGP, TCEANC, LCP2, METAP2, MAGEB4, CHST11, ZC3HC1, MCM10, DDX54, C8orf59, NAF1, FGF12, GSTM3, STAC, FAM64A, ATP6V0B, FLJ25758, CDC73, WWP2, CDH1, CEP131, PCM1, PPM1H, PTPN3, SSH1, TENC1, SMTNL2, PLEKHG5, SAMD4A, SPATA13, RIN1, TFEB, TESPA1, SAMD4B, FAM163A, CRTC2, FAM189A2, PAK6, RBM3, RAB3IP, CBY1, CEP170, INPP5E, SLC9A1, CYLD, COX15, DLD, DLST, DNM1L, SDHA, SOAT1, VDAC1, TRIM25, BRCA1, BRD1, BRMS1, YLR177W, ACM1, IFNAR1, CFTR, TARDBP, ZNF598, CTNNB1, MEX3C, EGLN3, RIPK4, PTPN4, API5, KRAS, PPP6C, COPE, GRHPR, JUP, PPIE, YAF2, PARD3B, RPA2, NIN, AKAP9, CASK, PRC1, RBM14, UBE2M, RAD18, EFTUD2, AAR2, PIH1D1, TNIP2, RNF31, HAVCR1, BPLF1, ESR2, HEXIM1, MEPCE, PPT1, AGR2, EZH2, RECQL4, KANK1, STUB1, BAP1, PIK3R1, GPC1, Prkaa1, Nav2, KIAA1429, RC3H1, RC3H2, ATG16L1, ACTC1, FAF1, CLIC4, FHL1, FHL2, FHL3, LMO1, LMO2, LMO3, TET2, KCTD15, GBF1, AGRN, BMH1, BMH2, ATXN3, HIBADH, DIABLO, NDUFAB1, ALDH1B1, ALDH2, COQ9, FH, HEXA, HSP90AA1, LDHB, MMP20, RAD23A, TIMM44, UBA1, VBP1, AARS2, ECH1, GRPEL1, IARS2, PAFAH2, PFDN4, SSBP1, DYRK1A, MAPT, CD74, SLC15A3, GEM, DUSP16, MTMR4, PTPDC1, ITFG1, BIRC3, NFX1, nsp7ab, ORF3a, CCDC125, PLEKHA4, PINK1, FANCD2, NGB, ZC3H18, CSK, PDPK1, PRKCE, PRKD1, SHC1, SHOC2, SFN, PHACTR4, MAP3K5, CHAF1B, CAMSAP3, LRCH1, SH2B3, CDK12, TANC2, EPN3, PANK2, MAP3K15, CEP89, FRMD6, THAP11, TTC17, CCNY, ARHGEF19, FAM122B, DAB2IP, FAM110C, LPIN1, PRR5, DNMT1, CDK11A, SRSF10, TBC1D7, NEDD4L, FAM117B, KANK2, DENND4C, RGS12, YWHAE, EMC4, ERC2, APPL2, ANKRD55, ESR1, SNAPIN, EDC3, NEK4, CHMP4C, CCR9, CYSLTR2, RXFP3, KIF14, RMDN3, HNRNPH1, INS, Rnf183, NUPR1, CIC, CCAR2, Apc2, RBM39, LGALS9, ACTN4, BTF3, CAD, CALD1, CDKN2A, COPA, DDB1, DDX6, DHX9, EIF2B1, GOLGA3, HDAC1, JAK1, LTBP1, PPP1R12A, NONO, PA2G4, PAWR, PRPS2, RBBP4, ROCK1, SMARCC2, TRIM21, TAF4, EIF3A, PABPC4, LRRFIP2, MTA2, VPRBP, EIF4A3, MATR3, HDAC6, SAE1, G3BP1, SMC2, IVNS1ABP, SF3B2, FASTKD2, NT5C2, STK38L, MGA, UBR2, CAND1, EFHD2, SHCBP1, SFPQ, TAB3, TAB1, ACAP2, INSIG1, NEDD4, SOD1, IL1B, UFL1, DDRGK1, KRT8, VAPA, FZR1, PAGE4, NUDCD2, LNP1, SNX33, FAM53B, SYNGAP1, CDR2L, GUCA1B, KIAA1211, ARHGEF4, FAM124A, EPB41L1, AFAP1L1, MIIP, PLEKHA3, GAREM, CD28, GPSM3, SIPA1L3, PDE7B, C19orf26, C6orf222, PSD4, MACC1, ZNF395, FAM86C1, SH2D3A, NCKIPSD, KSR2, SPRTN, TRIM26, FBXW7, FGD5, CCNF, HECTD1, NBR1, SQSTM1, MAP1LC3B, SLFN11, RIPK2, PER2,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
YWHAE
NUTM2A

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to YWHAE-NUTM2A

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to YWHAE-NUTM2A

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	YWHAE	C0036341	Schizophrenia	4	PSYGENET
Hgene	YWHAE	C0005586	Bipolar Disorder	2	PSYGENET
Hgene	YWHAE	C0206630	Endometrial Stromal Sarcoma	2	ORPHANET
Hgene	YWHAE	C0027627	Neoplasm Metastasis	1	CTD_human
Hgene	YWHAE	C0265219	Miller Dieker syndrome	1	ORPHANET
Hgene	YWHAE	C0334488	Clear cell sarcoma of kidney	1	ORPHANET
Hgene	YWHAE	C2750748	Chromosome 17p13.3 Duplication Syndrome	1	ORPHANET
Hgene	YWHAE	C4707092	Distal 17p13.3 microdeletion syndrome	1	ORPHANET
Tgene	NUTM2A	C0206630	Endometrial Stromal Sarcoma	2	ORPHANET