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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:CNOT2-SLC45A4

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: CNOT2-SLC45A4
	FusionPDB ID: 17751
	FusionGDB2.0 ID: 17751
		Hgene	Tgene
	Gene symbol	CNOT2	SLC45A4
	Gene ID	4848	57210
	Gene name	CCR4-NOT transcription complex subunit 2	solute carrier family 45 member 4
	Synonyms	CDC36\|HSPC131\|IDNADFS\|NOT2\|NOT2H	-
	Cytomap	12q15	8q24.3
	Type of gene	protein-coding	protein-coding
	Description	CCR4-NOT transcription complex subunit 2CCR4-associated factor 2negative regulator of transcription 2	solute carrier family 45 member 4
	Modification date	20200313	20200313
	UniProtAcc	Q9NZN8	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000229195, ENST00000418359, ENST00000548230, ENST00000551483,	ENST00000521804, ENST00000024061, ENST00000433583, ENST00000517878, ENST00000519067,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	41 X 13 X 11=5863	4 X 5 X 6=120
	# samples	46	8
	** MAII score	log2(46/5863*10)=-3.6719332904521 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/120*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: CNOT2 [Title/Abstract] AND SLC45A4 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	CNOT2(70713144)-SLC45A4(142231864), # samples:4
Anticipated loss of major functional domain due to fusion event.	CNOT2-SLC45A4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CNOT2-SLC45A4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	CNOT2	GO:0000122	negative regulation of transcription by RNA polymerase II	14707134\|16712523

Fusion gene breakpoints across CNOT2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across SLC45A4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-A7-A4SF-01A	CNOT2	chr12	70713144	-	SLC45A4	chr8	142231864	-
ChimerDB4	BRCA	TCGA-A7-A4SF-01A	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-
ChimerDB4	BRCA	TCGA-A7-A4SF	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000229195	CNOT2	chr12	70713144	+	ENST00000519067	SLC45A4	chr8	142231864	-	7908	817	540	2882	780
ENST00000229195	CNOT2	chr12	70713144	+	ENST00000433583	SLC45A4	chr8	142231864	-	4046	817	540	3002	820
ENST00000229195	CNOT2	chr12	70713144	+	ENST00000517878	SLC45A4	chr8	142231864	-	4046	817	540	3002	820
ENST00000229195	CNOT2	chr12	70713144	+	ENST00000024061	SLC45A4	chr8	142231864	-	3967	817	540	3125	861
ENST00000418359	CNOT2	chr12	70713144	+	ENST00000519067	SLC45A4	chr8	142231864	-	7780	689	412	2754	780
ENST00000418359	CNOT2	chr12	70713144	+	ENST00000433583	SLC45A4	chr8	142231864	-	3918	689	412	2874	820
ENST00000418359	CNOT2	chr12	70713144	+	ENST00000517878	SLC45A4	chr8	142231864	-	3918	689	412	2874	820
ENST00000418359	CNOT2	chr12	70713144	+	ENST00000024061	SLC45A4	chr8	142231864	-	3839	689	412	2997	861

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000229195	ENST00000519067	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.002093402	0.99790657
ENST00000229195	ENST00000433583	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.003873617	0.99612635
ENST00000229195	ENST00000517878	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.003873617	0.99612635
ENST00000229195	ENST00000024061	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.003948785	0.99605125
ENST00000418359	ENST00000519067	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.002118105	0.9978818
ENST00000418359	ENST00000433583	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.004026649	0.9959733
ENST00000418359	ENST00000517878	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.004026649	0.9959733
ENST00000418359	ENST00000024061	CNOT2	chr12	70713144	+	SLC45A4	chr8	142231864	-	0.004108113	0.9958918

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>17751_17751_1_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000229195_SLC45A4_chr8_142231864_ENST00000024061_length(amino acids)=861AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL
TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETERLQVLTSVRSRHIGWCRSCWRVFFFMIILEKKF

--------------------------------------------------------------

>17751_17751_2_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000229195_SLC45A4_chr8_142231864_ENST00000433583_length(amino acids)=820AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL
TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETESVTPAGIDVCQISSHWLVPQLLESIFLYDYFRK

--------------------------------------------------------------

>17751_17751_3_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000229195_SLC45A4_chr8_142231864_ENST00000517878_length(amino acids)=820AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL
TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETESVTPAGIDVCQISSHWLVPQLLESIFLYDYFRK

--------------------------------------------------------------

>17751_17751_4_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000229195_SLC45A4_chr8_142231864_ENST00000519067_length(amino acids)=780AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL

--------------------------------------------------------------

>17751_17751_5_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000418359_SLC45A4_chr8_142231864_ENST00000024061_length(amino acids)=861AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL
TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETERLQVLTSVRSRHIGWCRSCWRVFFFMIILEKKF

--------------------------------------------------------------

>17751_17751_6_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000418359_SLC45A4_chr8_142231864_ENST00000433583_length(amino acids)=820AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL
TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETESVTPAGIDVCQISSHWLVPQLLESIFLYDYFRK

--------------------------------------------------------------

>17751_17751_7_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000418359_SLC45A4_chr8_142231864_ENST00000517878_length(amino acids)=820AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL
TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETESVTPAGIDVCQISSHWLVPQLLESIFLYDYFRK

--------------------------------------------------------------

>17751_17751_8_CNOT2-SLC45A4_CNOT2_chr12_70713144_ENST00000418359_SLC45A4_chr8_142231864_ENST00000519067_length(amino acids)=780AA_BP=93
MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG
QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL
DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY
SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS
QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY
RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF
YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM
VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:70713144/chr8:142231864)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
CNOT2 Q9NZN8	.
FUNCTION: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity. {ECO:0000269\|PubMed:14707134, ECO:0000269\|PubMed:16712523, ECO:0000269\|PubMed:21299754, ECO:0000269\|PubMed:22367759}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

123_143

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

155_175

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

196_216

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

233_253

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

518_538

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

560_580

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

592_612

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

614_634

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

63_83

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

666_686

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

695_715

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000024061

86_106

29.333333333333332

799.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

123_143

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

155_175

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

196_216

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

233_253

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

518_538

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

560_580

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

592_612

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

614_634

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

63_83

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

666_686

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

695_715

29.333333333333332

718.0

Transmembrane

Helical

Tgene

SLC45A4

chr12:70713144

chr8:142231864

ENST00000519067

86_106

29.333333333333332

718.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

CNOT2

chr12:70713144

chr8:142231864

ENST00000229195

437_540

79.33333333333333

541.0

Region

Note=Repressor domain

Hgene

CNOT2

chr12:70713144

chr8:142231864

ENST00000418359

437_540

79.33333333333333

541.0

Region

Note=Repressor domain

Hgene

CNOT2

chr12:70713144

chr8:142231864

ENST00000551483

437_540

192.0

Region

Note=Repressor domain

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1652_CNOT2_70713144_SLC45A4_142231864_ranked_0.pdb	CNOT2	70713144	70713144	ENST00000024061	SLC45A4	chr8	142231864	-	MIASGTVRKGHDSMVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYG QQSLPEQYYSLTWFLSPILGLIFTPLIGSASDRCTLSWGRRRPFILALCVGVLFGVALFLNGSAIGLALGDVPNRQPIGIVLTVLGVVVL DFSADATEGPIRAYLLDVVDSEEQDMALNIHAFSAGLGGAIGYVLGGLDWTQTFLGSWFRTQNQVLFFFAAIIFTVSVALHLFSIDEEQY SPQQERSAEEPGALDGGEPHGVPAFPDEVQSEHELALDYPDVDIMRSKSDSALHVPDTALDLEPELLFLHDIEPSIFHDASYPATPRSTS QELAKTKLPRLATFLKEAAKEDETLLDNHLNEAKVPNGSGSPTKDALGGYTRVDTKPSATSSSMRRRRHAFRRQASSTFSYYGKLGSHCY RYRRANAVVLIKPSRSMSDLYDMQKRQRQHRHRNQSGATTSSGDTESEEGEGETTVRLLWLSMLKMPRELMRLCLCHLLTWFSVIAEAVF YTDFMGQVIFEGDPKAPSNSTAWQAYNAGVKMGCWGLVIYAATGAICSALLQKYLDNYDLSVRVIYVLGTLGFSVGTAVMAMFPNVYVAM VTISTMGIVSMSISYCPYALLGQYHDIKQYIHHSPGNSKRGFGIDCAILSCQVYISQILVASALGGVVDAVGTVRVIPMVASVGSFLGFL TATFLVIYPNVSEEAKEEQKGLSSPLAGEGRAGGNSEKPTVLKLTRKEGLQGPVETERLQVLTSVRSRHIGWCRSCWRVFFFMIILEKKF	861

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

CNOT2_pLDDT.png

SLC45A4_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
CNOT2
SLC45A4

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to CNOT2-SLC45A4

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to CNOT2-SLC45A4

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source