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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EIF2AK4-BUB1B

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EIF2AK4-BUB1B
	FusionPDB ID: 25675
	FusionGDB2.0 ID: 25675
		Hgene	Tgene
	Gene symbol	EIF2AK4	BUB1B
	Gene ID	440275	701
	Gene name	eukaryotic translation initiation factor 2 alpha kinase 4	BUB1 mitotic checkpoint serine/threonine kinase B
	Synonyms	GCN2\|PVOD2	BUB1beta\|BUBR1\|Bub1A\|MAD3L\|MVA1\|SSK1\|hBUBR1
	Cytomap	15q15.1	15q15.1
	Type of gene	protein-coding	protein-coding
	Description	eIF-2-alpha kinase GCN2GCN2 eIF2alpha kinaseGCN2-like proteingeneral control nonderepressible 2	mitotic checkpoint serine/threonine-protein kinase BUB1 betaBUB1B, mitotic checkpoint serine/threonine kinaseMAD3/BUB1-related protein kinasebudding uninhibited by benzimidazoles 1 homolog betamitotic checkpoint kinase MAD3L
	Modification date	20200313	20200313
	UniProtAcc	Q9P2K8	O60566
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000263791, ENST00000382727, ENST00000559311, ENST00000559624, ENST00000560648,	ENST00000560120, ENST00000287598, ENST00000412359,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	15 X 17 X 10=2550	8 X 10 X 5=400
	# samples	20	9
	** MAII score	log2(20/2550*10)=-3.6724253419715 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/400*10)=-2.15200309344505 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EIF2AK4 [Title/Abstract] AND BUB1B [Title/Abstract] AND fusion [Title/Abstract] Global analysis of somatic structural genomic alterations and their impact on gene expression in diverse human cancers (pmid: 27856756)
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	BUB1B(40453456)-EIF2AK4(40314720), # samples:2 EIF2AK4(40309443)-BUB1B(40504700), # samples:2 EIF2AK4(40309443)-BUB1B(40504699), # samples:2
Anticipated loss of major functional domain due to fusion event.	EIF2AK4-BUB1B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EIF2AK4-BUB1B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EIF2AK4-BUB1B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EIF2AK4-BUB1B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across EIF2AK4 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across BUB1B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-CH-5771-01A	EIF2AK4	chr15	40309442	+	BUB1B	chr15	40504699	+
ChimerDB4	PRAD	TCGA-CH-5771-01A	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504700	+
ChimerDB4	PRAD	TCGA-CH-5771	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504699	+
ChimerDB4	PRAD	TCGA-CH-5771	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504700	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000382727	EIF2AK4	chr15	40309443	+	ENST00000287598	BUB1B	chr15	40504700	+	5176	4031	50	4798	1582
ENST00000382727	EIF2AK4	chr15	40309443	+	ENST00000412359	BUB1B	chr15	40504700	+	5078	4031	50	4798	1582
ENST00000263791	EIF2AK4	chr15	40309443	+	ENST00000287598	BUB1B	chr15	40504700	+	5253	4108	43	4875	1610
ENST00000263791	EIF2AK4	chr15	40309443	+	ENST00000412359	BUB1B	chr15	40504700	+	5155	4108	43	4875	1610
ENST00000382727	EIF2AK4	chr15	40309443	+	ENST00000287598	BUB1B	chr15	40504699	+	5176	4031	50	4798	1582
ENST00000382727	EIF2AK4	chr15	40309443	+	ENST00000412359	BUB1B	chr15	40504699	+	5078	4031	50	4798	1582
ENST00000263791	EIF2AK4	chr15	40309443	+	ENST00000287598	BUB1B	chr15	40504699	+	5253	4108	43	4875	1610
ENST00000263791	EIF2AK4	chr15	40309443	+	ENST00000412359	BUB1B	chr15	40504699	+	5155	4108	43	4875	1610
ENST00000382727	EIF2AK4	chr15	40309442	+	ENST00000287598	BUB1B	chr15	40504699	+	5176	4031	50	4798	1582
ENST00000382727	EIF2AK4	chr15	40309442	+	ENST00000412359	BUB1B	chr15	40504699	+	5078	4031	50	4798	1582
ENST00000263791	EIF2AK4	chr15	40309442	+	ENST00000287598	BUB1B	chr15	40504699	+	5253	4108	43	4875	1610
ENST00000263791	EIF2AK4	chr15	40309442	+	ENST00000412359	BUB1B	chr15	40504699	+	5155	4108	43	4875	1610

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000382727	ENST00000287598	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504700	+	0.000269688	0.9997303
ENST00000382727	ENST00000412359	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504700	+	0.000351867	0.9996481
ENST00000263791	ENST00000287598	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504700	+	0.000642012	0.999358
ENST00000263791	ENST00000412359	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504700	+	0.000820554	0.9991794
ENST00000382727	ENST00000287598	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504699	+	0.000269688	0.9997303
ENST00000382727	ENST00000412359	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504699	+	0.000351867	0.9996481
ENST00000263791	ENST00000287598	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504699	+	0.000642012	0.999358
ENST00000263791	ENST00000412359	EIF2AK4	chr15	40309443	+	BUB1B	chr15	40504699	+	0.000820554	0.9991794
ENST00000382727	ENST00000287598	EIF2AK4	chr15	40309442	+	BUB1B	chr15	40504699	+	0.000269688	0.9997303
ENST00000382727	ENST00000412359	EIF2AK4	chr15	40309442	+	BUB1B	chr15	40504699	+	0.000351867	0.9996481
ENST00000263791	ENST00000287598	EIF2AK4	chr15	40309442	+	BUB1B	chr15	40504699	+	0.000642012	0.999358
ENST00000263791	ENST00000412359	EIF2AK4	chr15	40309442	+	BUB1B	chr15	40504699	+	0.000820554	0.9991794

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>25675_25675_1_EIF2AK4-BUB1B_EIF2AK4_chr15_40309442_ENST00000263791_BUB1B_chr15_40504699_ENST00000287598_length(amino acids)=1610AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL
RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG
MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP
TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA
VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS
FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF
IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG
RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH
GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ

--------------------------------------------------------------

>25675_25675_2_EIF2AK4-BUB1B_EIF2AK4_chr15_40309442_ENST00000263791_BUB1B_chr15_40504699_ENST00000412359_length(amino acids)=1610AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL
RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG
MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP
TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA
VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS
FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF
IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG
RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH
GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ

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>25675_25675_3_EIF2AK4-BUB1B_EIF2AK4_chr15_40309442_ENST00000382727_BUB1B_chr15_40504699_ENST00000287598_length(amino acids)=1582AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAY
IHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFS
LGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHT
LTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHS
GMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSI
YLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLV
KYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLVSSQPVPWDFYINLKLKERLNED
FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALK
IVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFV

--------------------------------------------------------------

>25675_25675_4_EIF2AK4-BUB1B_EIF2AK4_chr15_40309442_ENST00000382727_BUB1B_chr15_40504699_ENST00000412359_length(amino acids)=1582AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAY
IHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFS
LGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHT
LTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHS
GMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSI
YLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLV
KYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLVSSQPVPWDFYINLKLKERLNED
FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALK
IVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFV

--------------------------------------------------------------

>25675_25675_5_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000263791_BUB1B_chr15_40504699_ENST00000287598_length(amino acids)=1610AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL
RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG
MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP
TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA
VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS
FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF
IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG
RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH
GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ

--------------------------------------------------------------

>25675_25675_6_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000263791_BUB1B_chr15_40504699_ENST00000412359_length(amino acids)=1610AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL
RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG
MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP
TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA
VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS
FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF
IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG
RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH
GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ

--------------------------------------------------------------

>25675_25675_7_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000263791_BUB1B_chr15_40504700_ENST00000287598_length(amino acids)=1610AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL
RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG
MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP
TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA
VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS
FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF
IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG
RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH
GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ

--------------------------------------------------------------

>25675_25675_8_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000263791_BUB1B_chr15_40504700_ENST00000412359_length(amino acids)=1610AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL
RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG
MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP
TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA
VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS
FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF
IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG
RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH
GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ

--------------------------------------------------------------

>25675_25675_9_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000382727_BUB1B_chr15_40504699_ENST00000287598_length(amino acids)=1582AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAY
IHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFS
LGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHT
LTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHS
GMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSI
YLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLV
KYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLVSSQPVPWDFYINLKLKERLNED
FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALK
IVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFV

--------------------------------------------------------------

>25675_25675_10_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000382727_BUB1B_chr15_40504699_ENST00000412359_length(amino acids)=1582AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAY
IHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFS
LGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHT
LTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHS
GMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSI
YLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLV
KYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLVSSQPVPWDFYINLKLKERLNED
FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALK
IVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFV

--------------------------------------------------------------

>25675_25675_11_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000382727_BUB1B_chr15_40504700_ENST00000287598_length(amino acids)=1582AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAY
IHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFS
LGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHT
LTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHS
GMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSI
YLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLV
KYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLVSSQPVPWDFYINLKLKERLNED
FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALK
IVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFV

--------------------------------------------------------------

>25675_25675_12_EIF2AK4-BUB1B_EIF2AK4_chr15_40309443_ENST00000382727_BUB1B_chr15_40504700_ENST00000412359_length(amino acids)=1582AA_BP=0
MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI
ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ
RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF
NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR
YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL
ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN
PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF
RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS
ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAY
IHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFS
LGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHT
LTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHS
GMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSI
YLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLV
KYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLVSSQPVPWDFYINLKLKERLNED
FDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALK
IVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFV

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr15:40453456/chr15:40314720)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
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5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EIF2AK4 Q9P2K8	BUB1B O60566
FUNCTION: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability (PubMed:25329545). Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation (By similarity). EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion (By similarity). Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory (By similarity). Plays a role in neurite outgrowth inhibition (By similarity). Plays a proapoptotic role in response to glucose deprivation (By similarity). Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity). {ECO:0000250\|UniProtKB:P15442, ECO:0000250\|UniProtKB:Q9QZ05, ECO:0000269\|PubMed:25329545, ECO:0000269\|PubMed:26102367}.; FUNCTION: (Microbial infection) Plays a role in modulating the adaptive immune response to yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation (PubMed:24310610). {ECO:0000269\|PubMed:24310610}.	FUNCTION: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. {ECO:0000269\|PubMed:10477750, ECO:0000269\|PubMed:11702782, ECO:0000269\|PubMed:14706340, ECO:0000269\|PubMed:15020684, ECO:0000269\|PubMed:19411850, ECO:0000269\|PubMed:19503101}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

146_205

1355.0

1650.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

146_205

1327.0

1622.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

146_205

1355.0

1650.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

146_205

1327.0

1622.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

146_205

1355.0

1650.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

146_205

1327.0

1622.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

484_491

1355.0

1650.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

484_491

1327.0

1622.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

484_491

1355.0

1650.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

484_491

1327.0

1622.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

484_491

1355.0

1650.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

484_491

1327.0

1622.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

25_137

1355.0

1650.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

296_539

1355.0

1650.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

590_1001

1355.0

1650.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

25_137

1327.0

1622.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

296_539

1327.0

1622.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

590_1001

1327.0

1622.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

25_137

1355.0

1650.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

296_539

1355.0

1650.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

590_1001

1355.0

1650.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

25_137

1327.0

1622.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

296_539

1327.0

1622.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

590_1001

1327.0

1622.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

25_137

1355.0

1650.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

296_539

1355.0

1650.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

590_1001

1355.0

1650.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

25_137

1327.0

1622.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

296_539

1327.0

1622.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

590_1001

1327.0

1622.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

596_604

1355.0

1650.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

596_604

1327.0

1622.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

596_604

1355.0

1650.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

596_604

1327.0

1622.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

596_604

1355.0

1650.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

596_604

1327.0

1622.0

Nucleotide binding

ATP

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

146_205

617.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

146_205

617.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

146_205

617.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

484_491

617.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

484_491

617.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

484_491

617.0

Compositional bias

Note=Poly-Leu

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

25_137

617.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

296_539

617.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

590_1001

617.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

25_137

617.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

296_539

617.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

590_1001

617.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

25_137

617.0

Domain

RWD

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

296_539

617.0

Domain

Protein kinase 1

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

590_1001

617.0

Domain

Protein kinase 2

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

596_604

617.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

596_604

617.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

596_604

617.0

Nucleotide binding

ATP

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000263791

1022_1493

1355.0

1650.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000382727

1022_1493

1327.0

1622.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309442

chr15:40504699

ENST00000559624

1022_1493

617.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000263791

1022_1493

1355.0

1650.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000382727

1022_1493

1327.0

1622.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309443

chr15:40504699

ENST00000559624

1022_1493

617.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000263791

1022_1493

1355.0

1650.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000382727

1022_1493

1327.0

1622.0

Region

Note=Histidyl-tRNA synthetase-like

Hgene

EIF2AK4

chr15:40309443

chr15:40504700

ENST00000559624

1022_1493

617.0

Region

Note=Histidyl-tRNA synthetase-like

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000287598

209_215

795.0

1051.0

Compositional bias

Note=Poly-Glu

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000412359

209_215

809.0

1065.0

Compositional bias

Note=Poly-Glu

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000287598

209_215

795.0

1051.0

Compositional bias

Note=Poly-Glu

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000412359

209_215

809.0

1065.0

Compositional bias

Note=Poly-Glu

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000287598

209_215

795.0

1051.0

Compositional bias

Note=Poly-Glu

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000412359

209_215

809.0

1065.0

Compositional bias

Note=Poly-Glu

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000287598

62_226

795.0

1051.0

Domain

BUB1 N-terminal

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000287598

766_1050

795.0

1051.0

Domain

Note=Protein kinase

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000412359

62_226

809.0

1065.0

Domain

BUB1 N-terminal

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000412359

766_1050

809.0

1065.0

Domain

Note=Protein kinase

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000287598

62_226

795.0

1051.0

Domain

BUB1 N-terminal

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000287598

766_1050

795.0

1051.0

Domain

Note=Protein kinase

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000412359

62_226

809.0

1065.0

Domain

BUB1 N-terminal

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000412359

766_1050

809.0

1065.0

Domain

Note=Protein kinase

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000287598

62_226

795.0

1051.0

Domain

BUB1 N-terminal

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000287598

766_1050

795.0

1051.0

Domain

Note=Protein kinase

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000412359

62_226

809.0

1065.0

Domain

BUB1 N-terminal

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000412359

766_1050

809.0

1065.0

Domain

Note=Protein kinase

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000287598

111_118

795.0

1051.0

Motif

Nuclear localization signal

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000287598

224_232

795.0

1051.0

Motif

Note=D-box

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000412359

111_118

809.0

1065.0

Motif

Nuclear localization signal

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000412359

224_232

809.0

1065.0

Motif

Note=D-box

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000287598

111_118

795.0

1051.0

Motif

Nuclear localization signal

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000287598

224_232

795.0

1051.0

Motif

Note=D-box

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000412359

111_118

809.0

1065.0

Motif

Nuclear localization signal

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000412359

224_232

809.0

1065.0

Motif

Note=D-box

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000287598

111_118

795.0

1051.0

Motif

Nuclear localization signal

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000287598

224_232

795.0

1051.0

Motif

Note=D-box

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000412359

111_118

809.0

1065.0

Motif

Nuclear localization signal

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000412359

224_232

809.0

1065.0

Motif

Note=D-box

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000287598

772_780

795.0

1051.0

Nucleotide binding

ATP

Tgene

BUB1B

chr15:40309442

chr15:40504699

ENST00000412359

772_780

809.0

1065.0

Nucleotide binding

ATP

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000287598

772_780

795.0

1051.0

Nucleotide binding

ATP

Tgene

BUB1B

chr15:40309443

chr15:40504699

ENST00000412359

772_780

809.0

1065.0

Nucleotide binding

ATP

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000287598

772_780

795.0

1051.0

Nucleotide binding

ATP

Tgene

BUB1B

chr15:40309443

chr15:40504700

ENST00000412359

772_780

809.0

1065.0

Nucleotide binding

ATP

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>2099_EIF2AK4_40309443_BUB1B_40504700_ranked_0.pdb	EIF2AK4	40309442	40309443	ENST00000412359	BUB1B	chr15	40504700	+	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEI ELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQ RRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYF NMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVR YLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRL ADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFIN PQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQF RRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTL RDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTG MVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRP TATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA VQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNS FLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKF IEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGG RYDLLVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVH GDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQ	1610

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EIF2AK4_pLDDT.png

BUB1B_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EIF2AK4
BUB1B

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to EIF2AK4-BUB1B

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to EIF2AK4-BUB1B

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source