UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EPHB3-EPHB2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EPHB3-EPHB2
	FusionPDB ID: 27000
	FusionGDB2.0 ID: 27000
		Hgene	Tgene
	Gene symbol	EPHB3	EPHB2
	Gene ID	2049	2048
	Gene name	EPH receptor B3	EPH receptor B2
	Synonyms	EK2\|ETK2\|HEK2\|TYRO6	BDPLT22\|CAPB\|DRT\|EK5\|EPHT3\|ERK\|Hek5\|PCBC\|Tyro5
	Cytomap	3q27.1	1p36.12
	Type of gene	protein-coding	protein-coding
	Description	ephrin type-B receptor 3EPH-like tyrosine kinase 2embryonic kinase 2human embryo kinase 2tyrosine-protein kinase TYRO6	ephrin type-B receptor 2EPH-like kinase 5developmentally-regulated Eph-related tyrosine kinaseelk-related tyrosine kinaseeph tyrosine kinase 3protein-tyrosine kinase HEK5renal carcinoma antigen NY-REN-47tyrosine-protein kinase TYRO5tyrosine-protei
	Modification date	20200313	20200313
	UniProtAcc	P54753	P29323
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000330394,	ENST00000465676, ENST00000374627, ENST00000374630, ENST00000374632, ENST00000400191, ENST00000544305,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	4 X 4 X 4=64	10 X 10 X 5=500
	# samples	4	11
	** MAII score	log2(4/64*10)=-0.678071905112638 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(11/500*10)=-2.18442457113743 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EPHB3 [Title/Abstract] AND EPHB2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	EPHB3(184289170)-EPHB2(23110884), # samples:2
Anticipated loss of major functional domain due to fusion event.	EPHB3-EPHB2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EPHB3-EPHB2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EPHB3-EPHB2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EPHB3-EPHB2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	EPHB3	GO:0016477	cell migration	15536074
Hgene	EPHB3	GO:0022407	regulation of cell-cell adhesion	8397371
Hgene	EPHB3	GO:0034446	substrate adhesion-dependent cell spreading	15536074
Hgene	EPHB3	GO:0043087	regulation of GTPase activity	15536074
Hgene	EPHB3	GO:0046777	protein autophosphorylation	8397371\|15536074
Hgene	EPHB3	GO:0048013	ephrin receptor signaling pathway	8397371\|15536074

Fusion gene breakpoints across EPHB3 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across EPHB2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	Non-Cancer	231N	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+
ChimerDB4	Non-Cancer	43N	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000330394

EPHB3

chr3

184289170

ENST00000544305

EPHB2

chr1

23110884

2074

635

239

1957

572

ENST00000330394

EPHB3

chr3

184289170

ENST00000374630

EPHB2

chr1

23110884

3643

635

239

3469

1076

ENST00000330394

EPHB3

chr3

184289170

ENST00000400191

EPHB2

chr1

23110884

5132

635

239

3676

1145

ENST00000330394

EPHB3

chr3

184289170

ENST00000374632

EPHB2

chr1

23110884

4173

635

239

3472

1077

ENST00000330394

EPHB3

chr3

184289170

ENST00000374627

EPHB2

chr1

23110884

4517

635

239

3367

1042

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000330394	ENST00000544305	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+	0.022129154	0.9778708
ENST00000330394	ENST00000374630	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+	0.006397759	0.9936022
ENST00000330394	ENST00000400191	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+	0.002012493	0.9979875
ENST00000330394	ENST00000374632	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+	0.004858902	0.99514115
ENST00000330394	ENST00000374627	EPHB3	chr3	184289170	+	EPHB2	chr1	23110884	+	0.004222989	0.995777

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>27000_27000_1_EPHB3-EPHB2_EPHB3_chr3_184289170_ENST00000330394_EPHB2_chr1_23110884_ENST00000374627_length(amino acids)=1042AA_BP=49
MSASPGPPGSVHRLGPGAARLDCIPSSPGSPGTRREPAPEPAERTLSRVPAAPPARPGPARPGSAPRAATAMARARPPPPPSPPPGLLPL
LPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEM
KFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD
YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR
GCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI
ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD
SITLSWSQPDQPNGVILDYELQYYEKQELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQE
KLPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGA
GEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFT
VIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDV
WSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSG

--------------------------------------------------------------

>27000_27000_2_EPHB3-EPHB2_EPHB3_chr3_184289170_ENST00000330394_EPHB2_chr1_23110884_ENST00000374630_length(amino acids)=1076AA_BP=49
MSASPGPPGSVHRLGPGAARLDCIPSSPGSPGTRREPAPEPAERTLSRVPAAPPARPGPARPGSAPRAATAMARARPPPPPSPPPGLLPL
LPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEM
KFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD
YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR
GCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI
ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD
SITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEK
LPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAG
EFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTV
IQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVW
SYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGI

--------------------------------------------------------------

>27000_27000_3_EPHB3-EPHB2_EPHB3_chr3_184289170_ENST00000330394_EPHB2_chr1_23110884_ENST00000374632_length(amino acids)=1077AA_BP=49
MSASPGPPGSVHRLGPGAARLDCIPSSPGSPGTRREPAPEPAERTLSRVPAAPPARPGPARPGSAPRAATAMARARPPPPPSPPPGLLPL
LPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEM
KFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD
YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR
GCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI
ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD
SITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEK
LPLIIGSSAAGLVFLIAVVVIAIVCNRRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGA
GEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFT
VIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDV
WSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSG

--------------------------------------------------------------

>27000_27000_4_EPHB3-EPHB2_EPHB3_chr3_184289170_ENST00000330394_EPHB2_chr1_23110884_ENST00000400191_length(amino acids)=1145AA_BP=49
MSASPGPPGSVHRLGPGAARLDCIPSSPGSPGTRREPAPEPAERTLSRVPAAPPARPGPARPGSAPRAATAMARARPPPPPSPPPGLLPL
LPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEM
KFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD
YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR
GCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI
ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD
SITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEK
LPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAG
EFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTV
IQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVW
SYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGI
NLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEGQPLA

--------------------------------------------------------------

>27000_27000_5_EPHB3-EPHB2_EPHB3_chr3_184289170_ENST00000330394_EPHB2_chr1_23110884_ENST00000544305_length(amino acids)=572AA_BP=49
MSASPGPPGSVHRLGPGAARLDCIPSSPGSPGTRREPAPEPAERTLSRVPAAPPARPGPARPGSAPRAATAMARARPPPPPSPPPGLLPL
LPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEM
KFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD
YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR
GCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI
ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr3:184289170/chr1:23110884)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EPHB3 P54753	EPHB2 P29323
FUNCTION: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. {ECO:0000269\|PubMed:15536074}.	FUNCTION: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation (PubMed:30213874). {ECO:0000269\|PubMed:15300251, ECO:0000269\|PubMed:30213874}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

184_324

42.0

987.0

Compositional bias

Note=Cys-rich

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

184_324

42.0

988.0

Compositional bias

Note=Cys-rich

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

184_324

42.0

1056.0

Compositional bias

Note=Cys-rich

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

324_434

42.0

987.0

Domain

Fibronectin type-III 1

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

435_530

42.0

987.0

Domain

Fibronectin type-III 2

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

621_884

42.0

987.0

Domain

Protein kinase

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

913_977

42.0

987.0

Domain

SAM

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

324_434

42.0

988.0

Domain

Fibronectin type-III 1

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

435_530

42.0

988.0

Domain

Fibronectin type-III 2

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

621_884

42.0

988.0

Domain

Protein kinase

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

913_977

42.0

988.0

Domain

SAM

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

324_434

42.0

1056.0

Domain

Fibronectin type-III 1

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

435_530

42.0

1056.0

Domain

Fibronectin type-III 2

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

621_884

42.0

1056.0

Domain

Protein kinase

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

913_977

42.0

1056.0

Domain

SAM

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

984_986

42.0

987.0

Motif

PDZ-binding (in isoform 2)

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

984_986

42.0

988.0

Motif

PDZ-binding (in isoform 2)

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

984_986

42.0

1056.0

Motif

PDZ-binding (in isoform 2)

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

627_635

42.0

987.0

Nucleotide binding

ATP

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

627_635

42.0

988.0

Nucleotide binding

ATP

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

627_635

42.0

1056.0

Nucleotide binding

ATP

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

565_1055

42.0

987.0

Topological domain

Cytoplasmic

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

565_1055

42.0

988.0

Topological domain

Cytoplasmic

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

565_1055

42.0

1056.0

Topological domain

Cytoplasmic

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

544_564

42.0

987.0

Transmembrane

Helical

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

544_564

42.0

988.0

Transmembrane

Helical

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

544_564

42.0

1056.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

199_336

61.0

999.0

Compositional bias

Note=Cys-rich

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

339_451

61.0

999.0

Domain

Fibronectin type-III 1

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

39_217

61.0

999.0

Domain

Eph LBD

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

452_545

61.0

999.0

Domain

Fibronectin type-III 2

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

633_896

61.0

999.0

Domain

Protein kinase

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

925_989

61.0

999.0

Domain

SAM

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

996_998

61.0

999.0

Motif

PDZ-binding

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

639_647

61.0

999.0

Nucleotide binding

ATP

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

34_559

61.0

999.0

Topological domain

Extracellular

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

581_998

61.0

999.0

Topological domain

Cytoplasmic

Hgene

EPHB3

chr3:184289170

chr1:23110884

ENST00000330394

560_580

61.0

999.0

Transmembrane

Helical

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

20_202

42.0

987.0

Domain

Eph LBD

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

20_202

42.0

988.0

Domain

Eph LBD

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

20_202

42.0

1056.0

Domain

Eph LBD

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374630

19_543

42.0

987.0

Topological domain

Extracellular

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000374632

19_543

42.0

988.0

Topological domain

Extracellular

Tgene

EPHB2

chr3:184289170

chr1:23110884

ENST00000400191

19_543

42.0

1056.0

Topological domain

Extracellular

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1887_EPHB3_184289170_EPHB2_23110884_ranked_0.pdb	EPHB3	184289170	184289170	ENST00000374627	EPHB2	chr1	23110884	+	MSASPGPPGSVHRLGPGAARLDCIPSSPGSPGTRREPAPEPAERTLSRVPAAPPARPGPARPGSAPRAATAMARARPPPPPSPPPGLLPL LPPLLLLPLLLLPAGCRALEETLMDTKWVTSELAWTSHPESGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEM KFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQD YGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCR GCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNI ICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVD SITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEK LPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAG EFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTV IQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVW SYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGI NLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEGQPLA	1145

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EPHB3_pLDDT.png

EPHB2_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EPHB3
EPHB2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to EPHB3-EPHB2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to EPHB3-EPHB2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source