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level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MACF1-BRAF

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MACF1-BRAF
	FusionPDB ID: 50588
	FusionGDB2.0 ID: 50588
		Hgene	Tgene
	Gene symbol	MACF1	BRAF
	Gene ID	23499	673
	Gene name	microtubule actin crosslinking factor 1	B-Raf proto-oncogene, serine/threonine kinase
	Synonyms	ABP620\|ACF7\|LIS9\|MACF\|OFC4	B-RAF1\|B-raf\|BRAF1\|NS7\|RAFB1
	Cytomap	1p34.3	7q34
	Type of gene	protein-coding	protein-coding
	Description	microtubule-actin cross-linking factor 1620 kDa actin binding proteinactin cross-linking family protein 7macrophin 1 isoformtrabeculin-alpha	serine/threonine-protein kinase B-raf94 kDa B-raf proteinB-Raf proto-oncogene serine/threonine-protein kinase (p94)B-Raf serine/threonine-proteinmurine sarcoma viral (v-raf) oncogene homolog B1proto-oncogene B-Rafv-raf murine sarcoma viral oncogene
	Modification date	20200313	20200329
	UniProtAcc	Q9UPN3	P15056
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000289893, ENST00000317713, ENST00000361689, ENST00000372915, ENST00000539005, ENST00000545844, ENST00000564288, ENST00000567887, ENST00000476350, ENST00000484793, ENST00000536367, ENST00000602421,	ENST00000288602,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	46 X 37 X 13=22126	48 X 58 X 16=44544
	# samples	44	69
	** MAII score	log2(44/22126*10)=-5.65209532630239 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(69/44544*10)=-6.0124909441832 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MACF1 [Title/Abstract] AND BRAF [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MACF1(39896580)-BRAF(140487384), # samples:4 BRAF(140494108)-MACF1(39898207), # samples:3
Anticipated loss of major functional domain due to fusion event.	BRAF-MACF1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BRAF-MACF1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MACF1-BRAF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MACF1-BRAF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MACF1-BRAF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MACF1-BRAF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MACF1	GO:0030334	regulation of cell migration	27693509
Hgene	MACF1	GO:0043001	Golgi to plasma membrane protein transport	15265687
Hgene	MACF1	GO:0045773	positive regulation of axon extension	22705394
Hgene	MACF1	GO:0051893	regulation of focal adhesion assembly	27693509
Tgene	BRAF	GO:0000186	activation of MAPKK activity	29433126
Tgene	BRAF	GO:0006468	protein phosphorylation	17563371
Tgene	BRAF	GO:0010828	positive regulation of glucose transmembrane transport	23010278
Tgene	BRAF	GO:0033138	positive regulation of peptidyl-serine phosphorylation	19667065
Tgene	BRAF	GO:0043066	negative regulation of apoptotic process	19667065
Tgene	BRAF	GO:0070374	positive regulation of ERK1 and ERK2 cascade	22065586
Tgene	BRAF	GO:0071277	cellular response to calcium ion	18567582
Tgene	BRAF	GO:0090150	establishment of protein localization to membrane	23010278

Fusion gene breakpoints across MACF1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across BRAF (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	THCA	TCGA-DE-A0Y2-01A	MACF1	chr1	39896580	-	BRAF	chr7	140487384	-
ChimerDB4	THCA	TCGA-DE-A0Y2-01A	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-
ChimerDB4	THCA	TCGA-DE-A0Y2	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000545844	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	12538	11259	108	12419	4103
ENST00000567887	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	18884	17605	34	18765	6243
ENST00000372915	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	18391	17112	6	18272	6088
ENST00000361689	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	12482	11203	52	12363	4103
ENST00000539005	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	12092	10813	52	11973	3973
ENST00000317713	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	12482	11203	52	12363	4103
ENST00000564288	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	19393	18114	771	19274	6167
ENST00000289893	MACF1	chr1	39896580	+	ENST00000288602	BRAF	chr7	140487384	-	14067	12788	131	13948	4605

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000545844	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.004751456	0.9952486
ENST00000567887	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.002740972	0.997259
ENST00000372915	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.003544756	0.9964553
ENST00000361689	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.004488903	0.9955112
ENST00000539005	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.006168463	0.9938315
ENST00000317713	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.004488903	0.9955112
ENST00000564288	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.004397279	0.9956027
ENST00000289893	ENST00000288602	MACF1	chr1	39896580	+	BRAF	chr7	140487384	-	0.002639174	0.99736077

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>50588_50588_1_MACF1-BRAF_MACF1_chr1_39896580_ENST00000289893_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=4605AA_BP=4219
MQKGLLDQDTGLVLLESQVIMSGLIAPETGENLSLEEGIARNLINPQMYQQLRELQDALALISRLTESRGPLSVVEAIEKRIISETVGLK
ILEAHLATGGFSLSPSENCINLEEAFHQGLISAWLHSVLESYLRTSKNLIDPNTAEKIGLLDLMQRCIVHQESGFKLLPVKQLAGGMVSL
KSGRKVSIFRAVQEGLIDRQVTVRLLEAQLFAGGIVDPRTGHRLTVEEAVRHNLIDQDMACAILIRQLQTGGIIDTVTGQRLTIDEAVSN
DLVAAKIALVILESLWSFMGLLWPESGEILPITDALEQGIVSTELAHKILSNRQHIKALFLPATTEILSWKKAIESGILDRDLANNLKSI
CIPDVMPHMQLADSAEQNINPGAAVLPCSKSHPKATASQSENLLFQLMTHSYINVQNGQRLLLLDKELMETLTSRDEYQTSPPKVVEIGH
QRQKTPEGLQESANVKISGTFSSGWTVRLPEFQFSSQNKEYPDREDCTTEKGKKTTVETEDSSVENPEQDLFVEQKERNPNIDALKVINK
VKLEVQRQLIGTQREDQTAVSVRENASRGHLLTIPPAEAEGVPLVVDKDVFSVETPKKEHQPLRNTSFTCQNEQAHTLETEYIHDETGGS
HIKPQSKKLQVQVKKTLGIKLELKSETDGNVHPLDKKEMLKKTFLAKDDHKESQEAQNIAGGSMMMSEKTDEEDSGREIFLSCSHPLELL
EEATLNVLSAQLLDGGIFHEQTGQKLLLNEAISRGIVPSHTAVKLMEKLNMFQGFFDSQTCESLTTEEVINEGLMDEKLLHNVLMADKAI
SGVLDPRTQTLCSVKDAVTVGLLDKETATRILERQVVTGGIIDLKRGKKVSVTLASTLGLVDVADQPELINLEKASKGRDAEKTVRERLI
SLQMETTGLIDPDSKAPLTVVQSIDRGLLEREEAVRLLTKQVVDGGIIHHISGMRLSVDNAFRHGLIGEDLAEKLKRVENLNIHQIFNPE
TKENISLPKAIKLDLITSDLKREIQEVQAFTGNFVDLISGQRLTLAEAKKEGLLTNEAVLSPGMMHGIVDPENCRIVPYSELVKKCKIDI
ESGQRYLEVIPFSDIKDGVSDKVLTLSQAIQLGKVDFASTLKVLEAQANTGGIIDTATGKRLTLASALEEKLVDENMVRIIASHQVLNGG
IVDIFSDQRVTLVEAIEKRLISPELANMIQIDSSEFSDHRAQIEKQEGIEVCALQNEFLGKDMLIACNQTAEMSCNKVEESERLFQVENQ
SAQEKVKVRVSDGEQAKKSREISLKEFGCKDQRKPRMSSDAKEFISIINPHNLKGKSLGQVSLTHPYSECDFKLKEVARNNMGNDTNEEQ
EKAVTKIEIISHMKQSTSCLDSEEIRENQGEVILEVQETYCETSGKLPSEQVLQQPMNARVKSKREKREVIVEESIRTCKPAFLSEEKLY
QETAIRDEHDSHIKSQPREMTSSEKGKEADTEMGFSITFKIEESSSQVVPQGISVKHLDALTLFSSKQANEGKVNNLSLCLTLKPEENLS
REIACGAQSEPFPCMTPRPEGLHYQESDGKAQVTGPSQISKTDKSFQGTTRQETNYQDSWVTSKTKETKHQISSSNECKEKSYQEVSFDP
ARGLKLEEITVSRPDSKEVRYLEFSDRKDLHHQGSKSDDKLCGTLKSEIATQELTGEKFLEMANPNVAGLEAGSIEDIVTQRGSRVLGSF
LPEKLFKGVSQKENTGQQNAIISPTVLETSEEKTVSLTVCSAVKTEKTPQEKLRESPGSEQTPFMTAPEGKGNGGVNPEPFRATQNVFTR
QLCLEHDEKLVSYLSLLRNIEMRTKQIQPLELNLAELQDLLCQAKVLERELKDLTTLVSQELECVNQIIISQPQEVPAQLLKALEKDAKN
LQKSLSSVSDTWNSRLLHFQNAVEIEKTKVLNQHTQLEGRLQDLRAWVGNKNLILNSKGSNSEIDVDSLNLCLQQYEDLKQPMAERKAQL
DALAFDIQFFISEHAQDLSPQQNRQMLRLLNELQRSFQDILEQTAAQVDALQGHLQQMEQEALVKTLQKQQNTCHQQLEDLCSWVGQAER
ALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEENQTKLSPRELTALREKLHQAKEQYEALQEETRVAQKELE
EAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGMEQLSGASLEKGALDTTDGYMGVNQAPEKLDKQCEMMKAR
HQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSLAQSEAELKQVQTLQDELQKFLQDHKEFESWLERSEKEL
ENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFKEGKEPSEIGNLVKDKLKDATERYTALHSKCTRLGSHLN
MLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQLQEELAEHQVPVEKLQKVARDIMEIEGEPAPDHRHVQET
TDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEGKQVSSLSSGVIQEALATNMKLKQDIARQKSSLEATREM
VTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFEHLSGKLQQFMENKSRMLASGNQPDQDITHFFQQIQELN
LEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKEREKDASSCQEQLDEFRKLVRTFQKWLKETEGSIPPTET
SMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQGKTGSILPSVGSSVGSVNGYHTCKDLTEIQCDMSDVNLK
YEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSPTKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLL
VTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLSIDPNMLNAQKQQVQFMLK
EFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLNSRSSQIDQAIVKSTQYQELLQDLSEKVRAVGQRLSVQS
AISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDELKKRLETVALPLQGLEDLAADRINRLQAALASTQQFQQ
MFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEVIVAEGESLLLSVPPGEEKRTLQNQLVELKNHWEELSKK
TADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSLLRSKAMLNEVEKRRSLLEILNSAADILINSSEADEDGI
RDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKLRAQQEVLQALEPQVDYLR
NFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIGQFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIE
DVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKLTERGKARQEQLELTLGRVEDFYRKLKGLNDATTAAEEA
EALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGKDCDVQGLEHDMEEINARWNTLNKKVAQRIAQLQEALLH
CGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAELADREKITGQLESLESRWT
ELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTAKIQQQIIRHKALEEDIENHATDVHQAVKIGQSLSSLTS
PAEQGVLSEKIDSLQARYSEIQDRCCRKAALLDQALSNARLFGEDEVEVLNWLAEVEDKLSSVFVKDFKQDVLHRQHADHLALNEEIVNR
KKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLEQARQLATKFQSTYEELTGWLREVEEELATSGGQSPTGE
QIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDANEQYKLVSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGD
GGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVA
VKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKS
IIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINN
RDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASP

--------------------------------------------------------------

>50588_50588_2_MACF1-BRAF_MACF1_chr1_39896580_ENST00000317713_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=4103AA_BP=3717
MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNK
HLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHF
QISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRL
LDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFK
ETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLA
KNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPP
STLTTTHLKAEPLTKATHSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVE
ELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRN
YFSELTMELEEKQDVFRSLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENTAYFQFFSDARELESFLRNLQDSIK
RKYSCDHNTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISVKAVCDYRQIEITICKNDECVLEDNSQR
TKWKVISPTGNEAMVPSVCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYLRKDLDLVQTWNLEKLRSSAPGECHQIM
KNLQAHYEDFLQDSRDSVLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISELKNIRLRLEEYEQRVVKRIQSLASSRTD
RDAWQDNALRIAEQEHTQEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMDHVYGLSTVYLNKLKTVDVIVRSIQDAE
LLVKGYEIKLSQEEVVLADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSRLTPERNLDLERYQEKGSQLQERWHRVI
AQLEIRQSELESIQEVLGDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALFAEIERNQTKLDQCQKFSQQYSTIVKDY
ELQLMTYKAFVESQQKSPGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKELLGWVST
LARNTQGKATSSETKESTDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKEKKQISEQLNALNKAYHDLCDGSANQLQ
QLQSQLAHQTEQKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEEN
QTKLSPRELTALREKLHQAKEQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGME
QLSGASLEKGALDTTDGYMGVNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSL
AQSEAELKQVQTLQDELQKFLQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFK
EGKEPSEIGNLVKDKLKDATERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQL
QEELAEHQVPVEKLQKVARDIMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEG
KQVSSLSSGVIQEALATNMKLKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFE
HLSGKLQQFMENKSRMLASGNQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKE
REKDASSCQEQLDEFRKLVRTFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQG
KTGSILPSVGSSVGSVNGYHTCKDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSP
TKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAES
QLRPWLMEKELMMGVLGPLSIDPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLN
SRSSQIDQAIVKSTQYQELLQDLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDE
LKKRLETVALPLQGLEDLAADRINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEV
IVAEGESLLLSVPPGEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSL
LRSKAMLNEVEKRRSLLEILNSAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQ
LEIFDALGSQACSNKNLEKLRAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIG
QFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKL
TERGKARQEQLELTLGRVEDFYRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGK
DCDVQGLEHDMEEINARWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDR
KATVDMLQAEGGRIAQSAELADREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTA
KIQQQIIRHKALEEDIENHATDVHQAVKIGQSLSSLTSPAEQGVLSEKIDSLQARYSEIQDRCCRKAALLDQALSNARLFGEDEVEVLNW
LAEVEDKLSSVFVKDFKQDVLHRQHADHLALNEEIVNRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLE
QARQLATKFQSTYEELTGWLREVEEELATSGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDA
NEQYKLVSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGR
RDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCE
GSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPE
VIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILAS

--------------------------------------------------------------

>50588_50588_3_MACF1-BRAF_MACF1_chr1_39896580_ENST00000361689_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=4103AA_BP=3717
MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNK
HLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHF
QISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRL
LDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFK
ETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLA
KNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPP
STLTTTHLKAEPLTKATHSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVE
ELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRN
YFSELTMELEEKQDVFRSLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENTAYFQFFSDARELESFLRNLQDSIK
RKYSCDHNTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISVKAVCDYRQIEITICKNDECVLEDNSQR
TKWKVISPTGNEAMVPSVCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYLRKDLDLVQTWNLEKLRSSAPGECHQIM
KNLQAHYEDFLQDSRDSVLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISELKNIRLRLEEYEQRVVKRIQSLASSRTD
RDAWQDNALRIAEQEHTQEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMDHVYGLSTVYLNKLKTVDVIVRSIQDAE
LLVKGYEIKLSQEEVVLADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSRLTPERNLDLERYQEKGSQLQERWHRVI
AQLEIRQSELESIQEVLGDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALFAEIERNQTKLDQCQKFSQQYSTIVKDY
ELQLMTYKAFVESQQKSPGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKELLGWVST
LARNTQGKATSSETKESTDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKEKKQISEQLNALNKAYHDLCDGSANQLQ
QLQSQLAHQTEQKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEEN
QTKLSPRELTALREKLHQAKEQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGME
QLSGASLEKGALDTTDGYMGVNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSL
AQSEAELKQVQTLQDELQKFLQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFK
EGKEPSEIGNLVKDKLKDATERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQL
QEELAEHQVPVEKLQKVARDIMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEG
KQVSSLSSGVIQEALATNMKLKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFE
HLSGKLQQFMENKSRMLASGNQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKE
REKDASSCQEQLDEFRKLVRTFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQG
KTGSILPSVGSSVGSVNGYHTCKDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSP
TKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAES
QLRPWLMEKELMMGVLGPLSIDPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLN
SRSSQIDQAIVKSTQYQELLQDLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDE
LKKRLETVALPLQGLEDLAADRINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEV
IVAEGESLLLSVPPGEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSL
LRSKAMLNEVEKRRSLLEILNSAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQ
LEIFDALGSQACSNKNLEKLRAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIG
QFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKL
TERGKARQEQLELTLGRVEDFYRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGK
DCDVQGLEHDMEEINARWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDR
KATVDMLQAEGGRIAQSAELADREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTA
KIQQQIIRHKALEEDIENHATDVHQAVKIGQSLSSLTSPAEQGVLSEKIDSLQARYSEIQDRCCRKAALLDQALSNARLFGEDEVEVLNW
LAEVEDKLSSVFVKDFKQDVLHRQHADHLALNEEIVNRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLE
QARQLATKFQSTYEELTGWLREVEEELATSGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDA
NEQYKLVSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGR
RDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCE
GSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPE
VIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILAS

--------------------------------------------------------------

>50588_50588_4_MACF1-BRAF_MACF1_chr1_39896580_ENST00000372915_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=6088AA_BP=5702
MLTFTPFSFRSLLPGLPGPPAAAPAWAMSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLD
PAERAVVRVADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKL
VNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDM
ERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQH
TILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAV
ERLELLLQIANKIQNGALNCEEKLTLAKNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQ
DELVTLRLECTNLYRKGHFTSLELVPPSTLTTTHLKAEPLTKATHSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKL
ERAEWGNDLPSVELQLETQQHIHTSVEELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAEL
IWLNEKEEEELAYDWSDNNSNISAKRNYFSELTMELEEKQDVFRSLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVK
ENTAYFQFFSDARELESFLRNLQDSIKRKYSCDHNTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISV
KAVCDYRQIEITICKNDECVLEDNSQRTKWKVISPTGNEAMVPSVCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYL
RKDLDLVQTWNLEKLRSSAPGECHQIMKNLQAHYEDFLQDSRDSVLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISEL
KNIRLRLEEYEQRVVKRIQSLASSRTDRDAWQDNALRIAEQEHTQEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMD
HVYGLSTVYLNKLKTVDVIVRSIQDAELLVKGYEIKLSQEEVVLADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSR
LTPERNLDLERYQEKGSQLQERWHRVIAQLEIRQSELESIQEVLGDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALF
AEIERNQTKLDQCQKFSQQYSTIVKDYELQLMTYKAFVESQQKSPGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRL
EEEEKVVEEEKQEHVEKVKELLGWVSTLARNTQGKATSSETKESTDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKE
KKQISEQLNALNKAYHDLCDGSANQLQQLQSQLAHQTEQKECRAVAGVIDLGTVEIFPIFKAMQKGLLDQDTGLVLLESQVIMSGLIAPE
TGENLSLEEGIARNLINPQMYQQLRELQDALALISRLTESRGPLSVVEAIEKRIISETVGLKILEAHLATGGFSLSPSENCINLEEAFHQ
GLISAWLHSVLESYLRTSKNLIDPNTAEKIGLLDLMQRCIVHQESGFKLLPVKQLAGGMVSLKSGRKVSIFRAVQEGLIDRQVTVRLLEA
QLFAGGIVDPRTGHRLTVEEAVRHNLIDQDMACAILIRQLQTGGIIDTVTGQRLTIDEAVSNDLVAAKIALVILESLWSFMGLLWPESGE
ILPITDALEQGIVSTELAHKILSNRQHIKALFLPATTEILSWKKAIESGILDRDLANNLKSICIPDVMPHMQLADSAEQNINPGAAVLPC
SKSHPKATASQSENLLFQLMTHSYINVQNGQRLLLLDKELMETLTSRDEYQTSPPKVVEIGHQRQKTPEGLQESANVKISGTFSSGWTVR
LPEFQFSSQNKEYPDREDCTTEKGKKTTVETEDSSVENPEQDLFVEQKERNPNIDALKVINKVKLEVQRQLIGTQREDQTAVSVRENASR
GHLLTIPPAEAEGVPLVVDKDVFSVETPKKEHQPLRNTSFTCQNEQAHTLETEYIHDETGGSHIKPQSKKLQVQVKKTLGIKLELKSETD
GNVHPLDKKEMLKKTFLAKDDHKESQEAQNIAGGSMMMSEKTDEEDSGREIFLSCSHPLELLEEATLNVLSAQLLDGGIFHEQTGQKLLL
NEAISRGIVPSHTAVKLMEKLNMFQGFFDSQTCESLTTEEVINEGLMDEKLLHNVLMADKAISGVLDPRTQTLCSVKDAVTVGLLDKETA
TRILERQVVTGGIIDLKRGKKVSVTLASTLGLVDVADQPELINLEKASKGRDAEKTVRERLISLQMETTGLIDPDSKAPLTVVQSIDRGL
LEREEAVRLLTKQVVDGGIIHHISGMRLSVDNAFRHGLIGEDLAEKLKRVENLNIHQIFNPETKENISLPKAIKLDLITSDLKREIQEVQ
AFTGNFVDLISGQRLTLAEAKKEGLLTNEAVLSPGMMHGIVDPENCRIVPYSELVKKCKIDIESGQRYLEVIPFSDIKDGVSDKVLTLSQ
AIQLGKVDFASTLKVLEAQANTGGIIDTATGKRLTLASALEEKLVDENMVRIIASHQVLNGGIVDIFSDQRVTLVEAIEKRLISPELANM
IQIDSSEFSDHRAQIEKQEGIEVCALQNEFLGKDMLIACNQTAEMSCNKVEESERLFQVENQSAQEKVKVRVSDGEQAKKSREISLKEFG
CKDQRKPRMSSDAKEFISIINPHNLKGKSLGQVSLTHPYSECDFKLKEVARNNMGNDTNEEQEKAVTKIEIISHMKQSTSCLDSEEIREN
QGEVILEVQETYCETSGKLPSEQVLQQPMNARVKSKREKREVIVEESIRTCKPAFLSEEKLYQETAIRDEHDSHIKSQPREMTSSEKGKE
ADTEMGFSITFKIEESSSQVVPQGISVKHLDALTLFSSKQANEGKVNNLSLCLTLKPEENLSREIACGAQSEPFPCMTPRPEGLHYQESD
GKAQVTGPSQISKTDKSFQGTTRQETNYQDSWVTSKTKETKHQISSSNECKEKSYQEVSFDPARGLKLEEITVSRPDSKEVRYLEFSDRK
DLHHQGSKSDDKLCGTLKSEIATQELTGEKFLEMANPNVAGLEAGSIEDIVTQRGSRVLGSFLPEKLFKGVSQKENTGQQNAIISPTVLE
TSEEKTVSLTVCSAVKTEKTPQEKLRESPGSEQTPFMTAPEGKGNGGVNPEPFRATQNVFTRQLCLEHDEKLVSYLSLLRNIEMRTKQIQ
PLELNLAELQDLLCQAKVLERELKDLTTLVSQELECVNQIIISQPQEVPAQLLKALEKDAKNLQKSLSSVSDTWNSRLLHFQNAVEIEKT
KVLNQHTQLEGRLQDLRAWVGNKNLILNSKGSNSEIDVDSLNLCLQQYEDLKQPMAERKAQLDALAFDIQFFISEHAQDLSPQQNRQMLR
LLNELQRSFQDILEQTAAQVDALQGHLQQMEQEALVKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQ
DDIQNRATSFATVVKDIEGFMEENQTKLSPRELTALREKLHQAKEQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKID
ALLDWVTSVGSSGGQLLTNLPGMEQLSGASLEKGALDTTDGYMGVNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHN
LTPEEQQMLQQKLGELKEQYSTSLAQSEAELKQVQTLQDELQKFLQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVI
SHKGDLRFVTISGQKVLDMENSFKEGKEPSEIGNLVKDKLKDATERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVE
KLLSDTVASDPGVLQEQLATTKQLQEELAEHQVPVEKLQKVARDIMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQ
SQSVQESLESLLQSIGEVEQNLEGKQVSSLSSGVIQEALATNMKLKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFE
QLCLQQQEKESSLKKLLPQAEMFEHLSGKLQQFMENKSRMLASGNQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALD
LCQHQDRVQNLRKDFTELQKTVKEREKDASSCQEQLDEFRKLVRTFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLV
EEINCKGTSLENLIMEITAPDSQGKTGSILPSVGSSVGSVNGYHTCKDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKE
ANSVLQWLESKEEVLKSMDAMSSPTKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATE
VTVARQRQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLSIDPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSL
STSQVQKELQSINQKWVELTDKLNSRSSQIDQAIVKSTQYQELLQDLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDH
EVKEAQTLCDELSVLIGEQYLKDELKKRLETVALPLQGLEDLAADRINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLER
LQSQLQENEEFQKSLNQHSGSYEVIVAEGESLLLSVPPGEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPW
IEDCKAKMSELRVTLDPVQLESSLLRSKAMLNEVEKRRSLLEILNSAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEM
TQRLREFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKLRAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQ
EFLEVKQRVNSGCVMMENKLEGIGQFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLE
EEGTLDLLGLKRELEALNKQCGKLTERGKARQEQLELTLGRVEDFYRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQ
VDPLQMKLQQVNGLGQGLIQSAGKDCDVQGLEHDMEEINARWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPP
SAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAELADREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAE
PISDFLSVTEKKLANSEPVGTQTAKIQQQIIRHKALNEEIVNRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKA
LRTLEQARQLATKFQSTYEELTGWLREVEEELATSGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDK
LVSDANEQYKLVSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRM
KTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIV
TQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSIL
WMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFP

--------------------------------------------------------------

>50588_50588_5_MACF1-BRAF_MACF1_chr1_39896580_ENST00000539005_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=3973AA_BP=3587
MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNK
HLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHF
QISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRL
LDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFK
ETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLA
KNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPP
STLTTTHLKAEPLTKATHSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVE
ELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRN
YFSELTMELEEKQDVFRSLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENTAYFQFFSDARELESFLRNLQDSIK
RKYSCDHNTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISVKAVCDYRQIEITICKNDECVLEDNSQR
TKWKVISPTGNEAMVPSVCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYLRKDLDLVQTWNLEKLRSSAPGECHQIM
KNLQAHYEDFLQDSRDSVLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISELKNIRLRLEEYEQRVVKRIQSLASSRTD
RDAWQDNALRIAEQEHTQEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMDHVYGLSTVYLNKLKTVDVIVRSIQDAE
LLVKGYEIKLSQEEVVLADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSRLTPERNLDLERYQEKGSQLQERWHRVI
AQLEIRQSELESIQEVLGDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALFAEIERNQTKLDQCQKFSQQYSTIVKDY
ELQLMTYKAFVESQQKSPGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKELLGWVST
LARNTQGKATSSETKESTDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKEKKQISEQLNALNKAYHDLCDGSANQLQ
QLQSQLAHQTEQKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEEN
QTKLSPRELTALREKLHQAKEQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGME
QLSGASLEKGALDTTDGYMGVNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSL
AQSEAELKQVQTLQDELQKFLQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFK
EGKEPSEIGNLVKDKLKDATERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQL
QEELAEHQVPVEKLQKVARDIMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEG
KQVSSLSSGVIQEALATNMKLKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFE
HLSGKLQQFMENKSRMLASGNQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKE
REKDASSCQEQLDEFRKLVRTFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQG
KTDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSPTKTETVKAQAESNKAFLAELE
QNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLSI
DPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLNSRSSQIDQAIVKSTQYQELLQ
DLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDELKKRLETVALPLQGLEDLAAD
RINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEVIVAEGESLLLSVPPGEEKRTL
QNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSLLRSKAMLNEVEKRRSLLEILN
SAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKLR
AQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIGQFHCRVREMFSQLADLDDELD
GMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKLTERGKARQEQLELTLGRVEDF
YRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGKDCDVQGLEHDMEEINARWNTL
NKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAELA
DREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTAKIQQQIIRHKALNEEIVNRKK
NVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLEQARQLATKFQSTYEELTGWLREVEEELATSGGQSPTGEQI
PQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDANEQYKLVSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGDGG
STTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVK
MLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSII
HRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD
QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKT

--------------------------------------------------------------

>50588_50588_6_MACF1-BRAF_MACF1_chr1_39896580_ENST00000545844_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=4103AA_BP=3717
MSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPLHEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNK
HLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHF
QISDIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRL
LDAEDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFK
ETEILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLA
KNTLQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPP
STLTTTHLKAEPLTKATHSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVE
ELGSSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRN
YFSELTMELEEKQDVFRSLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENTAYFQFFSDARELESFLRNLQDSIK
RKYSCDHNTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISVKAVCDYRQIEITICKNDECVLEDNSQR
TKWKVISPTGNEAMVPSVCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYLRKDLDLVQTWNLEKLRSSAPGECHQIM
KNLQAHYEDFLQDSRDSVLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISELKNIRLRLEEYEQRVVKRIQSLASSRTD
RDAWQDNALRIAEQEHTQEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMDHVYGLSTVYLNKLKTVDVIVRSIQDAE
LLVKGYEIKLSQEEVVLADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSRLTPERNLDLERYQEKGSQLQERWHRVI
AQLEIRQSELESIQEVLGDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALFAEIERNQTKLDQCQKFSQQYSTIVKDY
ELQLMTYKAFVESQQKSPGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKELLGWVST
LARNTQGKATSSETKESTDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKEKKQISEQLNALNKAYHDLCDGSANQLQ
QLQSQLAHQTEQKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEEN
QTKLSPRELTALREKLHQAKEQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGME
QLSGASLEKGALDTTDGYMGVNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSL
AQSEAELKQVQTLQDELQKFLQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFK
EGKEPSEIGNLVKDKLKDATERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQL
QEELAEHQVPVEKLQKVARDIMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEG
KQVSSLSSGVIQEALATNMKLKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFE
HLSGKLQQFMENKSRMLASGNQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKE
REKDASSCQEQLDEFRKLVRTFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQG
KTGSILPSVGSSVGSVNGYHTCKDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSP
TKTETVKAQAESNKAFLAELEQNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAES
QLRPWLMEKELMMGVLGPLSIDPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLN
SRSSQIDQAIVKSTQYQELLQDLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDE
LKKRLETVALPLQGLEDLAADRINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEV
IVAEGESLLLSVPPGEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSL
LRSKAMLNEVEKRRSLLEILNSAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQ
LEIFDALGSQACSNKNLEKLRAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIG
QFHCRVREMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKL
TERGKARQEQLELTLGRVEDFYRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGK
DCDVQGLEHDMEEINARWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDR
KATVDMLQAEGGRIAQSAELADREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTA
KIQQQIIRHKALEEDIENHATDVHQAVKIGQSLSSLTSPAEQGVLSEKIDSLQARYSEIQDRCCRKAALLDQALSNARLFGEDEVEVLNW
LAEVEDKLSSVFVKDFKQDVLHRQHADHLALNEEIVNRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLE
QARQLATKFQSTYEELTGWLREVEEELATSGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDA
NEQYKLVSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGR
RDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCE
GSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPE
VIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILAS

--------------------------------------------------------------

>50588_50588_7_MACF1-BRAF_MACF1_chr1_39896580_ENST00000564288_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=6167AA_BP=5781
MEMPLLDSSYLPPTIFILTHVLGVAGVLYWKRHARGRADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLE
PAGLKTLRLVSMPSWCHTNNEEQAEEDDDDVPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQIS
DIYISGESGDMSAKEKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDA
EDVDVPSPDEKSVITYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETE
ILAKEREKGRIEELYKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLAKNT
LQADAAHLESGQPVQCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPPSTL
TTTHLKAEPLTKATHSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVEELG
SSVKEARLYEGKMSQNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRNYFS
ELTMELEEKQDVFRSLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENTAYFQFFSDARELESFLRNLQDSIKRKY
SCDHNTSLSRLEDLLQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISVKAVCDYRQIEITICKNDECVLEDNSQRTKW
KVISPTGNEAMVPSVCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYLRKDLDLVQTWNLEKLRSSAPGECHQIMKNL
QAHYEDFLQDSRDSVLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISELKNIRLRLEEYEQRVVKRIQSLASSRTDRDA
WQDNALRIAEQEHTQEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMDHVYGLSTVYLNKLKTVDVIVRSIQDAELLV
KGYEIKLSQEEVVLADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSRLTPERNLDLERYQEKGSQLQERWHRVIAQL
EIRQSELESIQEVLGDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALFAEIERNQTKLDQCQKFSQQYSTIVKDYELQ
LMTYKAFVESQQKSPGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKELLGWVSTLAR
NTQGKATSSETKESTDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKEKKQISEQLNALNKAYHDLCDGSANQLQQLQ
SQLAHQTEQKECRAVAGVIDLGTVEIFPIFKAMQKGLLDQDTGLVLLESQVIMSGLIAPETGENLSLEEGIARNLINPQMYQQLRELQDA
LALISRLTESRGPLSVVEAIEKRIISETVGLKILEAHLATGGFSLSPSENCINLEEAFHQGLISAWLHSVLESYLRTSKNLIDPNTAEKI
GLLDLMQRCIVHQESGFKLLPVKQLAGGMVSLKSGRKVSIFRAVQEGLIDRQVTVRLLEAQLFAGGIVDPRTGHRLTVEEAVRHNLIDQD
MACAILIRQLQTGGIIDTVTGQRLTIDEAVSNDLVAAKIALVILESLWSFMGLLWPESGEILPITDALEQGIVSTELAHKILSNRQHIKA
LFLPATTEILSWKKAIESGILDRDLANNLKSICIPDVMPHMQLADSAEQNINPGAAVLPCSKSHPKATASQSENLLFQLMTHSYINVQNG
QRLLLLDKELMETLTSRDEYQTSPPKVVEIGHQRQKTPEGLQESANVKISGTFSSGWTVRLPEFQFSSQNKEYPDREDCTTEKGKKTTVE
TEDSSVENPEQDLFVEQKERNPNIDALKVINKVKLEVQRQLIGTQREDQTAVSVRENASRGHLLTIPPAEAEGVPLVVDKDVFSVETPKK
EHQPLRNTSFTCQNEQAHTLETEYIHDETGGSHIKPQSKKLQVQVKKTLGIKLELKSETDGNVHPLDKKEMLKKTFLAKDDHKESQEAQN
IAGGSMMMSEKTDEEDSGREIFLSCSHPLELLEEATLNVLSAQLLDGGIFHEQTGQKLLLNEAISRGIVPSHTAVKLMEKLNMFQGFFDS
QTCESLTTEEVINEGLMDEKLLHNVLMADKAISGVLDPRTQTLCSVKDAVTVGLLDKETATRILERQVVTGGIIDLKRGKKVSVTLASTL
GLVDVADQPELINLEKASKGRDAEKTVRERLISLQMETTGLIDPDSKAPLTVVQSIDRGLLEREEAVRLLTKQVVDGGIIHHISGMRLSV
DNAFRHGLIGEDLAEKLKRVENLNIHQIFNPETKENISLPKAIKLDLITSDLKREIQEVQAFTGNFVDLISGQRLTLAEAKKEGLLTNEA
VLSPGMMHGIVDPENCRIVPYSELVKKCKIDIESGQRYLEVIPFSDIKDGVSDKVLTLSQAIQLGKVDFASTLKVLEAQANTGGIIDTAT
GKRLTLASALEEKLVDENMVRIIASHQVLNGGIVDIFSDQRVTLVEAIEKRLISPELANMIQIDSSEFSDHRAQIEKQEGIEVCALQNEF
LGKDMLIACNQTAEMSCNKVEESERLFQVENQSAQEKVKVRVSDGEQAKKSREISLKEFGCKDQRKPRMSSDAKEFISIINPHNLKGKSL
GQVSLTHPYSECDFKLKEVARNNMGNDTNEEQEKAVTKIEIISHMKQSTSCLDSEEIRENQGEVILEVQETYCETSGKLPSEQVLQQPMN
ARVKSKREKREVIVEESIRTCKPAFLSEEKLYQETAIRDEHDSHIKSQPREMTSSEKGKEADTEMGFSITFKIEESSSQVVPQGISVKHL
DALTLFSSKQANEGKVNNLSLCLTLKPEENLSREIACGAQSEPFPCMTPRPEGLHYQESDGKAQVTGPSQISKTDKSFQGTTRQETNYQD
SWVTSKTKETKHQISSSNECKEKSYQEVSFDPARGLKLEEITVSRPDSKEVRYLEFSDRKDLHHQGSKSDDKLCGTLKSEIATQELTGEK
FLEMANPNVAGLEAGSIEDIVTQRGSRVLGSFLPEKLFKGVSQKENTGQQNAIISPTVLETSEEKTVSLTVCSAVKTEKTPQEKLRESPG
SEQTPFMTAPEGKGNGGVNPEPFRATQNVFTRQLCLEHDEKLVSYLSLLRNIEMRTKQIQPLELNLAELQDLLCQAKVLERELKDLTTLV
SQELECVNQIIISQPQEVPAQLLKALEKDAKNLQKSLSSVSDTWNSRLLHFQNAVEIEKTKVLNQHTQLEGRLQDLRAWVGNKNLILNSK
GSNSEIDVDSLNLCLQQYEDLKQPMAERKAQLDALAFDIQFFISEHAQDLSPQQNRQMLRLLNELQRSFQDILEQTAAQVDALQGHLQQM
EQEALVKTLQKQQNTCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEENQTKLSP
RELTALREKLHQAKEQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGMEQLSGAS
LEKGALDTTDGYMGVNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSLAQSEAE
LKQVQTLQDELQKFLQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFKEGKEPS
EIGNLVKDKLKDATERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQLQEELAE
HQVPVEKLQKVARDIMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEGKQVSSL
SSGVIQEALATNMKLKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFEHLSGKL
QQFMENKSRMLASGNQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKEREKDAS
SCQEQLDEFRKLVRTFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQGKTGSIL
PSVGSSVGSVNGYHTCKDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSPTKTETV
KAQAESNKAFLAELEQNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAESQLRPWL
MEKELMMGVLGPLSIDPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLNSRSSQI
DQAIVKSTQYQELLQDLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDELKKRLE
TVALPLQGLEDLAADRINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEVIVAEGE
SLLLSVPPGEEKRTLQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSLLRSKAM
LNEVEKRRSLLEILNSAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQLEIFDA
LGSQACSNKNLEKLRAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIGQFHCRV
REMFSQLADLDDELDGMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKLTERGKA
RQEQLELTLGRVEDFYRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGKDCDVQG
LEHDMEEINARWNTLNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDM
LQAEGGRIAQSAELADREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTAKIQQQI
IRHKALEEDIENHATDVHQAVKIGQSLSSLTSPAEQGVLSEKIDSLQARYSEIQDRCCRKAALLDQALSNARLFGEDEVEVLNWLAEVED
KLSSVFVKDFKQDVLHRQHADHLALNEEIVNRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLEQARQLA
TKFQSTYEELTGWLREVEEELATSGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDANEQYKL
VSDTIGQRVDEIDAAIQRSQQDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDD
WEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYH
HLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQD
KNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLAR

--------------------------------------------------------------

>50588_50588_8_MACF1-BRAF_MACF1_chr1_39896580_ENST00000567887_BRAF_chr7_140487384_ENST00000288602_length(amino acids)=6243AA_BP=5857
MLPKSQCSLWDPFCLLTFTPFSFRSLLPGLPGPPAAAPAWAMSSSDEETLSERSCRSERSCRSERSYRSERSGSLSPCPPGDTLPWNLPL
HEQKKRKSQDSVLDPAERAVVRVADERDRVQKKTFTKWVNKHLMKVRKHINDLYEDLRDGHNLISLLEVLSGIKLEPAGLKTLRLVSMPS
WCHTNNEEQAEEDDDDVPREKGRMRFHRLQNVQIALDFLKQRQVKLVNIRNDDITDGNPKLTLGLIWTIILHFQISDIYISGESGDMSAK
EKLLLWTQKVTAGYTGIKCTNFSSCWSDGKMFNALIHRYRPDLVDMERVQIQSNRENLEQAFEVAERLGVTRLLDAEDVDVPSPDEKSVI
TYVSSIYDAFPKVPEGGEGISATEVDSRWQEYQSRVDSLIPWIKQHTILMSDKTFPQNPVELKALYNQYIHFKETEILAKEREKGRIEEL
YKLLEVWIEFGRIKLPQGYHPNDVEEEWGKLIIEMLEREKSLRPAVERLELLLQIANKIQNGALNCEEKLTLAKNTLQADAAHLESGQPV
QCESDVIMYIQECEGLIRQLQVDLQILRDENYYQLEELAFRVMRLQDELVTLRLECTNLYRKGHFTSLELVPPSTLTTTHLKAEPLTKAT
HSSSTSWFRKPMTRAELVAISSSEDEGNLRFVYELLSWVEEMQMKLERAEWGNDLPSVELQLETQQHIHTSVEELGSSVKEARLYEGKMS
QNFHTSYAETLGKLETQYCKLKETSSFRMRHLQSLHKFVSRATAELIWLNEKEEEELAYDWSDNNSNISAKRNYFSELTMELEEKQDVFR
SLQDTAELLSLENHPAKQTVEAYSAAVQSQLQWMKQLCLCVEQHVKENTAYFQFFSDARELESFLRNLQDSIKRKYSCDHNTSLSRLEDL
LQDSMDEKEQLIQSKSSVASLVGRSKTIVQLKPRSPDHVLKNTISVKAVCDYRQIEITICKNDECVLEDNSQRTKWKVISPTGNEAMVPS
VCFLIPPPNKDAIEMASRVEQSYQKVMALWHQLHVNTKSLISWNYLRKDLDLVQTWNLEKLRSSAPGECHQIMKNLQAHYEDFLQDSRDS
VLFSVADRLRLEEEVEACKARFQHLMKSMENEDKEETVAKMYISELKNIRLRLEEYEQRVVKRIQSLASSRTDRDAWQDNALRIAEQEHT
QEDLQQLRSDLDAVSMKCDSFLHQSPSSSSVPTLRSELNLLVEKMDHVYGLSTVYLNKLKTVDVIVRSIQDAELLVKGYEIKLSQEEVVL
ADLSALEAHWSTLRHWLSDVKDKNSVFSVLDEEIAKAKVVAEQMSRLTPERNLDLERYQEKGSQLQERWHRVIAQLEIRQSELESIQEVL
GDYRACHGTLIKWIEETTAQQEMMKPGQAEDSRVLSEQLSQQTALFAEIERNQTKLDQCQKFSQQYSTIVKDYELQLMTYKAFVESQQKS
PGKRRRMLSSSDAITQEFMDLRTRYTALVTLTTQHVKYISDALRRLEEEEKVVEEEKQEHVEKVKELLGWVSTLARNTQGKATSSETKES
TDIEKAILEQQVLSEELTTKKEQVSEAIKTSQIFLAKHGHKLSEKEKKQISEQLNALNKAYHDLCDGSANQLQQLQSQLAHQTEQKECRA
VAGVIDLGTVEIFPIFKAMQKGLLDQDTGLVLLESQVIMSGLIAPETGENLSLEEGIARNLINPQMYQQLRELQDALALISRLTESRGPL
SVVEAIEKRIISETVGLKILEAHLATGGFSLSPSENCINLEEAFHQGLISAWLHSVLESYLRTSKNLIDPNTAEKIGLLDLMQRCIVHQE
SGFKLLPVKQLAGGMVSLKSGRKVSIFRAVQEGLIDRQVTVRLLEAQLFAGGIVDPRTGHRLTVEEAVRHNLIDQDMACAILIRQLQTGG
IIDTVTGQRLTIDEAVSNDLVAAKIALVILESLWSFMGLLWPESGEILPITDALEQGIVSTELAHKILSNRQHIKALFLPATTEILSWKK
AIESGILDRDLANNLKSICIPDVMPHMQLADSAEQNINPGAAVLPCSKSHPKATASQSENLLFQLMTHSYINVQNGQRLLLLDKELMETL
TSRDEYQTSPPKVVEIGHQRQKTPEGLQESANVKISGTFSSGWTVRLPEFQFSSQNKEYPDREDCTTEKGKKTTVETEDSSVENPEQDLF
VEQKERNPNIDALKVINKVKLEVQRQLIGTQREDQTAVSVRENASRGHLLTIPPAEAEGVPLVVDKDVFSVETPKKEHQPLRNTSFTCQN
EQAHTLETEYIHDETGGSHIKPQSKKLQVQVKKTLGIKLELKSETDGNVHPLDKKEMLKKTFLAKDDHKESQEAQNIAGGSMMMSEKTDE
EDSGREIFLSCSHPLELLEEATLNVLSAQLLDGGIFHEQTGQKLLLNEAISRGIVPSHTAVKLMEKLNMFQGFFDSQTCESLTTEEVINE
GLMDEKLLHNVLMADKAISGVLDPRTQTLCSVKDAVTVGLLDKETATRILERQVVTGGIIDLKRGKKVSVTLASTLGLVDVADQPELINL
EKASKGRDAEKTVRERLISLQMETTGLIDPDSKAPLTVVQSIDRGLLEREEAVRLLTKQVVDGGIIHHISGMRLSVDNAFRHGLIGEDLA
EKLKRVENLNIHQIFNPETKENISLPKAIKLDLITSDLKREIQEVQAFTGNFVDLISGQRLTLAEAKKEGLLTNEAVLSPGMMHGIVDPE
NCRIVPYSELVKKCKIDIESGQRYLEVIPFSDIKDGVSDKVLTLSQAIQLGKVDFASTLKVLEAQANTGGIIDTATGKRLTLASALEEKL
VDENMVRIIASHQVLNGGIVDIFSDQRVTLVEAIEKRLISPELANMIQIDSSEFSDHRAQIEKQEGIEVCALQNEFLGKDMLIACNQTAE
MSCNKVEESERLFQVENQSAQEKVKVRVSDGEQAKKSREISLKEFGCKDQRKPRMSSDAKEFISIINPHNLKGKSLGQVSLTHPYSECDF
KLKEVARNNMGNDTNEEQEKAVTKIEIISHMKQSTSCLDSEEIRENQGEVILEVQETYCETSGKLPSEQVLQQPMNARVKSKREKREVIV
EESIRTCKPAFLSEEKLYQETAIRDEHDSHIKSQPREMTSSEKGKEADTEMGFSITFKIEESSSQVVPQGISVKHLDALTLFSSKQANEG
KVNNLSLCLTLKPEENLSREIACGAQSEPFPCMTPRPEGLHYQESDGKAQVTGPSQISKTDKSFQGTTRQETNYQDSWVTSKTKETKHQI
SSSNECKEKSYQEVSFDPARGLKLEEITVSRPDSKEVRYLEFSDRKDLHHQGSKSDDKLCGTLKSEIATQELTGEKFLEMANPNVAGLEA
GSIEDIVTQRGSRVLGSFLPEKLFKGVSQKENTGQQNAIISPTVLETSEEKTVSLTVCSAVKTEKTPQEKLRESPGSEQTPFMTAPEGKG
NGGVNPEPFRATQNVFTRQLCLEHDEKLVSYLSLLRNIEMRTKQIQPLELNLAELQDLLCQAKVLERELKDLTTLVSQELECVNQIIISQ
PQEVPAQLLKALEKDAKNLQKSLSSVSDTWNSRLLHFQNAVEIEKTKVLNQHTQLEGRLQDLRAWVGNKNLILNSKGSNSEIDVDSLNLC
LQQYEDLKQPMAERKAQLDALAFDIQFFISEHAQDLSPQQNRQMLRLLNELQRSFQDILEQTAAQVDALQGHLQQMEQEALVKTLQKQQN
TCHQQLEDLCSWVGQAERALAGHQGRTTQQDLSALQKNQSDLKDLQDDIQNRATSFATVVKDIEGFMEENQTKLSPRELTALREKLHQAK
EQYEALQEETRVAQKELEEAVTSALQQETEKSKAAKELAENKKKIDALLDWVTSVGSSGGQLLTNLPGMEQLSGASLEKGALDTTDGYMG
VNQAPEKLDKQCEMMKARHQELLSQQQNFILATQSAQAFLDQHGHNLTPEEQQMLQQKLGELKEQYSTSLAQSEAELKQVQTLQDELQKF
LQDHKEFESWLERSEKELENMHKGGSSPETLPSLLKRQGSFSEDVISHKGDLRFVTISGQKVLDMENSFKEGKEPSEIGNLVKDKLKDAT
ERYTALHSKCTRLGSHLNMLLGQYHQFQNSADSLQAWMQACEANVEKLLSDTVASDPGVLQEQLATTKQLQEELAEHQVPVEKLQKVARD
IMEIEGEPAPDHRHVQETTDSILSHFQSLSYSLAERSSLLQKAIAQSQSVQESLESLLQSIGEVEQNLEGKQVSSLSSGVIQEALATNMK
LKQDIARQKSSLEATREMVTRFMETADSTTAAVLQGKLAEVSQRFEQLCLQQQEKESSLKKLLPQAEMFEHLSGKLQQFMENKSRMLASG
NQPDQDITHFFQQIQELNLEMEDQQENLDTLEHLVTELSSCGFALDLCQHQDRVQNLRKDFTELQKTVKEREKDASSCQEQLDEFRKLVR
TFQKWLKETEGSIPPTETSMSAKELEKQIEHLKSLLDDWASKGTLVEEINCKGTSLENLIMEITAPDSQGKTGSILPSVGSSVGSVNGYH
TCKDLTEIQCDMSDVNLKYEKLGGVLHERQESLQAILNRMEEVHKEANSVLQWLESKEEVLKSMDAMSSPTKTETVKAQAESNKAFLAEL
EQNSPKIQKVKEALAGLLVTYPNSQEAENWKKIQEELNSRWERATEVTVARQRQLEESASHLACFQAAESQLRPWLMEKELMMGVLGPLS
IDPNMLNAQKQQVQFMLKEFEARRQQHEQLNEAAQGILTGPGDVSLSTSQVQKELQSINQKWVELTDKLNSRSSQIDQAIVKSTQYQELL
QDLSEKVRAVGQRLSVQSAISTQPEAVKQQLEETSEIRSDLEQLDHEVKEAQTLCDELSVLIGEQYLKDELKKRLETVALPLQGLEDLAA
DRINRLQAALASTQQFQQMFDELRTWLDDKQSQQAKNCPISAKLERLQSQLQENEEFQKSLNQHSGSYEVIVAEGESLLLSVPPGEEKRT
LQNQLVELKNHWEELSKKTADRQSRLKDCMQKAQKYQWHVEDLVPWIEDCKAKMSELRVTLDPVQLESSLLRSKAMLNEVEKRRSLLEIL
NSAADILINSSEADEDGIRDEKAGINQNMDAVTEELQAKTGSLEEMTQRLREFQESFKNIEKKVEGAKHQLEIFDALGSQACSNKNLEKL
RAQQEVLQALEPQVDYLRNFTQGLVEDAPDGSDASQLLHQAEVAQQEFLEVKQRVNSGCVMMENKLEGIGQFHCRVREMFSQLADLDDEL
DGMGAIGRDTDSLQSQIEDVRLFLNKIHVLKLDIEASEAECRHMLEEEGTLDLLGLKRELEALNKQCGKLTERGKARQEQLELTLGRVED
FYRKLKGLNDATTAAEEAEALQWVVGTEVEIINQQLADFKMFQKEQVDPLQMKLQQVNGLGQGLIQSAGKDCDVQGLEHDMEEINARWNT
LNKKVAQRIAQLQEALLHCGKFQDALEPLLSWLADTEELIANQKPPSAEYKVVKAQIQEQKLLQRLLDDRKATVDMLQAEGGRIAQSAEL
ADREKITGQLESLESRWTELLSKAAARQKQLEDILVLAKQFHETAEPISDFLSVTEKKLANSEPVGTQTAKIQQQIIRHKALEEDIENHA
TDVHQAVKIGQSLSSLTSPAEQGVLSEKIDSLQARYSEIQDRCCRKAALLDQALSNARLFGEDEVEVLNWLAEVEDKLSSVFVKDFKQDV
LHRQHADHLALNEEIVNRKKNVDQAIKNGQALLKQTTGEEVLLIQEKLDGIKTRYADITVTSSKALRTLEQARQLATKFQSTYEELTGWL
REVEEELATSGGQSPTGEQIPQFQQRQKELKKEVMEHRLVLDTVNEVSRALLELVPWRAREGLDKLVSDANEQYKLVSDTIGQRVDEIDA
AIQRSQQDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRI
GSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKL
IDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFG
IVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSL

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:39896580/chr7:140487384)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MACF1 Q9UPN3	BRAF P15056
FUNCTION: [Isoform 2]: F-actin-binding protein which plays a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules (PubMed:15265687, PubMed:20937854). Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854). Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane (By similarity). Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics (By similarity). Interaction with CAMSAP3 at the minus ends of non-centrosomal microtubules tethers microtubules minus-ends to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4 (PubMed:15265687). Plays a key role in wound healing and epidermal cell migration (By similarity). Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate microtubule dynamics and polarize hair follicle stem cells (By similarity). As a regulator of actin and microtubule arrangement and stabilization, it plays an essential role in neurite outgrowth, branching and spine formation during brain development (By similarity). {ECO:0000250\|UniProtKB:Q9QXZ0, ECO:0000269\|PubMed:15265687, ECO:0000269\|PubMed:20937854, ECO:0000269\|PubMed:27693509}.	FUNCTION: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179). {ECO:0000269\|PubMed:1508179, ECO:0000269\|PubMed:21441910, ECO:0000269\|PubMed:29433126, ECO:0000305}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1122_1127

4219.0

5939.0

Compositional bias

Note=Poly-Ser

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1414_1417

4219.0

5939.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1986_1989

4219.0

5939.0

Compositional bias

Note=Poly-Leu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

698_703

4219.0

5939.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1122_1127

3717.0

5431.0

Compositional bias

Note=Poly-Ser

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1414_1417

3717.0

5431.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1986_1989

3717.0

5431.0

Compositional bias

Note=Poly-Leu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

698_703

3717.0

5431.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1122_1127

5675.0

7389.0

Compositional bias

Note=Poly-Ser

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1414_1417

5675.0

7389.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1986_1989

5675.0

7389.0

Compositional bias

Note=Poly-Leu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

698_703

5675.0

7389.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1122_1127

3717.0

5431.0

Compositional bias

Note=Poly-Ser

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1414_1417

3717.0

5431.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1986_1989

3717.0

5431.0

Compositional bias

Note=Poly-Leu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

698_703

3717.0

5431.0

Compositional bias

Note=Poly-Glu

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

194_298

4219.0

5939.0

Domain

Calponin-homology (CH) 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

78_181

4219.0

5939.0

Domain

Calponin-homology (CH) 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

868_925

4219.0

5939.0

Domain

SH3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

194_298

3717.0

5431.0

Domain

Calponin-homology (CH) 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

78_181

3717.0

5431.0

Domain

Calponin-homology (CH) 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

868_925

3717.0

5431.0

Domain

SH3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

194_298

5675.0

7389.0

Domain

Calponin-homology (CH) 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

78_181

5675.0

7389.0

Domain

Calponin-homology (CH) 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

868_925

5675.0

7389.0

Domain

SH3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

194_298

3717.0

5431.0

Domain

Calponin-homology (CH) 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

78_181

3717.0

5431.0

Domain

Calponin-homology (CH) 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

868_925

3717.0

5431.0

Domain

SH3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1_295

4219.0

5939.0

Region

Note=Actin-binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1_295

3717.0

5431.0

Region

Note=Actin-binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1_295

5675.0

7389.0

Region

Note=Actin-binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1_295

3717.0

5431.0

Region

Note=Actin-binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1050_1073

4219.0

5939.0

Repeat

Note=LRR 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1128_1154

4219.0

5939.0

Repeat

Note=LRR 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1187_1210

4219.0

5939.0

Repeat

Note=LRR 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1257_1282

4219.0

5939.0

Repeat

Note=LRR 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

148_171

4219.0

5939.0

Repeat

Note=LRR 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1577_1621

4219.0

5939.0

Repeat

Note=Plectin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1654_1696

4219.0

5939.0

Repeat

Note=Plectin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1769_1809

4219.0

5939.0

Repeat

Note=Plectin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1811_1848

4219.0

5939.0

Repeat

Note=Plectin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

1855_1886

4219.0

5939.0

Repeat

Note=Plectin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

2290_2332

4219.0

5939.0

Repeat

Note=Plectin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

2367_2410

4219.0

5939.0

Repeat

Note=Plectin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

240_264

4219.0

5939.0

Repeat

Note=LRR 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

2411_2437

4219.0

5939.0

Repeat

Note=Plectin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

2501_2543

4219.0

5939.0

Repeat

Note=Plectin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

2581_2612

4219.0

5939.0

Repeat

Note=Plectin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

2686_2730

4219.0

5939.0

Repeat

Note=Plectin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

3239_3262

4219.0

5939.0

Repeat

Note=LRR 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

3264_3283

4219.0

5939.0

Repeat

Note=LRR 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

3646_3669

4219.0

5939.0

Repeat

Note=LRR 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

3696_3720

4219.0

5939.0

Repeat

Note=LRR 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

377_399

4219.0

5939.0

Repeat

Note=LRR 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

3883_3957

4219.0

5939.0

Repeat

Note=Spectrin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

3936_3958

4219.0

5939.0

Repeat

Note=LRR 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4000_4108

4219.0

5939.0

Repeat

Note=Spectrin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4125_4150

4219.0

5939.0

Repeat

Note=LRR 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

441_464

4219.0

5939.0

Repeat

Note=LRR 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1050_1073

3717.0

5431.0

Repeat

Note=LRR 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1128_1154

3717.0

5431.0

Repeat

Note=LRR 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1187_1210

3717.0

5431.0

Repeat

Note=LRR 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1257_1282

3717.0

5431.0

Repeat

Note=LRR 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

148_171

3717.0

5431.0

Repeat

Note=LRR 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1577_1621

3717.0

5431.0

Repeat

Note=Plectin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1654_1696

3717.0

5431.0

Repeat

Note=Plectin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1769_1809

3717.0

5431.0

Repeat

Note=Plectin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1811_1848

3717.0

5431.0

Repeat

Note=Plectin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

1855_1886

3717.0

5431.0

Repeat

Note=Plectin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

2290_2332

3717.0

5431.0

Repeat

Note=Plectin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

2367_2410

3717.0

5431.0

Repeat

Note=Plectin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

240_264

3717.0

5431.0

Repeat

Note=LRR 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

2411_2437

3717.0

5431.0

Repeat

Note=Plectin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

2501_2543

3717.0

5431.0

Repeat

Note=Plectin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

2581_2612

3717.0

5431.0

Repeat

Note=Plectin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

2686_2730

3717.0

5431.0

Repeat

Note=Plectin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

3239_3262

3717.0

5431.0

Repeat

Note=LRR 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

3264_3283

3717.0

5431.0

Repeat

Note=LRR 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

3646_3669

3717.0

5431.0

Repeat

Note=LRR 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

3696_3720

3717.0

5431.0

Repeat

Note=LRR 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

377_399

3717.0

5431.0

Repeat

Note=LRR 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

441_464

3717.0

5431.0

Repeat

Note=LRR 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1050_1073

5675.0

7389.0

Repeat

Note=LRR 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1128_1154

5675.0

7389.0

Repeat

Note=LRR 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1187_1210

5675.0

7389.0

Repeat

Note=LRR 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1257_1282

5675.0

7389.0

Repeat

Note=LRR 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

148_171

5675.0

7389.0

Repeat

Note=LRR 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1577_1621

5675.0

7389.0

Repeat

Note=Plectin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1654_1696

5675.0

7389.0

Repeat

Note=Plectin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1769_1809

5675.0

7389.0

Repeat

Note=Plectin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1811_1848

5675.0

7389.0

Repeat

Note=Plectin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

1855_1886

5675.0

7389.0

Repeat

Note=Plectin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

2290_2332

5675.0

7389.0

Repeat

Note=Plectin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

2367_2410

5675.0

7389.0

Repeat

Note=Plectin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

240_264

5675.0

7389.0

Repeat

Note=LRR 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

2411_2437

5675.0

7389.0

Repeat

Note=Plectin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

2501_2543

5675.0

7389.0

Repeat

Note=Plectin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

2581_2612

5675.0

7389.0

Repeat

Note=Plectin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

2686_2730

5675.0

7389.0

Repeat

Note=Plectin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

3239_3262

5675.0

7389.0

Repeat

Note=LRR 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

3264_3283

5675.0

7389.0

Repeat

Note=LRR 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

3646_3669

5675.0

7389.0

Repeat

Note=LRR 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

3696_3720

5675.0

7389.0

Repeat

Note=LRR 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

377_399

5675.0

7389.0

Repeat

Note=LRR 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

3883_3957

5675.0

7389.0

Repeat

Note=Spectrin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

3936_3958

5675.0

7389.0

Repeat

Note=LRR 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4000_4108

5675.0

7389.0

Repeat

Note=Spectrin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4125_4150

5675.0

7389.0

Repeat

Note=LRR 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4261_4287

5675.0

7389.0

Repeat

Note=LRR 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

441_464

5675.0

7389.0

Repeat

Note=LRR 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4466_4574

5675.0

7389.0

Repeat

Note=Spectrin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4511_4534

5675.0

7389.0

Repeat

Note=LRR 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4601_4624

5675.0

7389.0

Repeat

Note=LRR 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4769_4792

5675.0

7389.0

Repeat

Note=LRR 18

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4800_4904

5675.0

7389.0

Repeat

Note=Spectrin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

4909_5012

5675.0

7389.0

Repeat

Note=Spectrin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5051_5076

5675.0

7389.0

Repeat

Note=LRR 19

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5172_5194

5675.0

7389.0

Repeat

Note=LRR 20

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5236_5341

5675.0

7389.0

Repeat

Note=Spectrin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5281_5304

5675.0

7389.0

Repeat

Note=LRR 21

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5348_5450

5675.0

7389.0

Repeat

Note=Spectrin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5455_5557

5675.0

7389.0

Repeat

Note=Spectrin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1050_1073

3717.0

5431.0

Repeat

Note=LRR 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1128_1154

3717.0

5431.0

Repeat

Note=LRR 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1187_1210

3717.0

5431.0

Repeat

Note=LRR 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1257_1282

3717.0

5431.0

Repeat

Note=LRR 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

148_171

3717.0

5431.0

Repeat

Note=LRR 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1577_1621

3717.0

5431.0

Repeat

Note=Plectin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1654_1696

3717.0

5431.0

Repeat

Note=Plectin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1769_1809

3717.0

5431.0

Repeat

Note=Plectin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1811_1848

3717.0

5431.0

Repeat

Note=Plectin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

1855_1886

3717.0

5431.0

Repeat

Note=Plectin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

2290_2332

3717.0

5431.0

Repeat

Note=Plectin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

2367_2410

3717.0

5431.0

Repeat

Note=Plectin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

240_264

3717.0

5431.0

Repeat

Note=LRR 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

2411_2437

3717.0

5431.0

Repeat

Note=Plectin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

2501_2543

3717.0

5431.0

Repeat

Note=Plectin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

2581_2612

3717.0

5431.0

Repeat

Note=Plectin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

2686_2730

3717.0

5431.0

Repeat

Note=Plectin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

3239_3262

3717.0

5431.0

Repeat

Note=LRR 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

3264_3283

3717.0

5431.0

Repeat

Note=LRR 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

3646_3669

3717.0

5431.0

Repeat

Note=LRR 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

3696_3720

3717.0

5431.0

Repeat

Note=LRR 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

377_399

3717.0

5431.0

Repeat

Note=LRR 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

441_464

3717.0

5431.0

Repeat

Note=LRR 4

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

428_432

380.0

767.0

Compositional bias

Note=Poly-Ser

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

457_717

380.0

767.0

Domain

Protein kinase

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

463_471

380.0

767.0

Nucleotide binding

ATP

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7054_7065

4219.0

5939.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7090_7101

4219.0

5939.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7054_7065

3717.0

5431.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7090_7101

3717.0

5431.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7054_7065

5675.0

7389.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7090_7101

5675.0

7389.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7054_7065

3717.0

5431.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7090_7101

3717.0

5431.0

Calcium binding

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7222_7351

4219.0

5939.0

Compositional bias

Note=Ser-rich

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7222_7351

3717.0

5431.0

Compositional bias

Note=Ser-rich

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7222_7351

5675.0

7389.0

Compositional bias

Note=Ser-rich

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7222_7351

3717.0

5431.0

Compositional bias

Note=Ser-rich

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7041_7076

4219.0

5939.0

Domain

EF-hand 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7077_7112

4219.0

5939.0

Domain

EF-hand 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7117_7189

4219.0

5939.0

Domain

GAR

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7041_7076

3717.0

5431.0

Domain

EF-hand 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7077_7112

3717.0

5431.0

Domain

EF-hand 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7117_7189

3717.0

5431.0

Domain

GAR

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7041_7076

5675.0

7389.0

Domain

EF-hand 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7077_7112

5675.0

7389.0

Domain

EF-hand 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7117_7189

5675.0

7389.0

Domain

GAR

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7041_7076

3717.0

5431.0

Domain

EF-hand 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7077_7112

3717.0

5431.0

Domain

EF-hand 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7117_7189

3717.0

5431.0

Domain

GAR

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7117_7388

4219.0

5939.0

Region

C-terminal tail

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

7313_7328

4219.0

5939.0

Region

Note=4 X 4 AA tandem repeats of [GS]-S-R-[AR]

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7117_7388

3717.0

5431.0

Region

C-terminal tail

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

7313_7328

3717.0

5431.0

Region

Note=4 X 4 AA tandem repeats of [GS]-S-R-[AR]

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7117_7388

5675.0

7389.0

Region

C-terminal tail

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

7313_7328

5675.0

7389.0

Region

Note=4 X 4 AA tandem repeats of [GS]-S-R-[AR]

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7117_7388

3717.0

5431.0

Region

C-terminal tail

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

7313_7328

3717.0

5431.0

Region

Note=4 X 4 AA tandem repeats of [GS]-S-R-[AR]

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4261_4287

4219.0

5939.0

Repeat

Note=LRR 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4466_4574

4219.0

5939.0

Repeat

Note=Spectrin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4511_4534

4219.0

5939.0

Repeat

Note=LRR 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4601_4624

4219.0

5939.0

Repeat

Note=LRR 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4769_4792

4219.0

5939.0

Repeat

Note=LRR 18

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4800_4904

4219.0

5939.0

Repeat

Note=Spectrin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

4909_5012

4219.0

5939.0

Repeat

Note=Spectrin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5051_5076

4219.0

5939.0

Repeat

Note=LRR 19

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5172_5194

4219.0

5939.0

Repeat

Note=LRR 20

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5236_5341

4219.0

5939.0

Repeat

Note=Spectrin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5281_5304

4219.0

5939.0

Repeat

Note=LRR 21

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5348_5450

4219.0

5939.0

Repeat

Note=Spectrin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5455_5557

4219.0

5939.0

Repeat

Note=Spectrin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5695_5719

4219.0

5939.0

Repeat

Note=LRR 22

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5783_5885

4219.0

5939.0

Repeat

Note=Spectrin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

5804_5828

4219.0

5939.0

Repeat

Note=LRR 23

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6005_6110

4219.0

5939.0

Repeat

Note=Spectrin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6115_6219

4219.0

5939.0

Repeat

Note=Spectrin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6225_6328

4219.0

5939.0

Repeat

Note=Spectrin 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6333_6439

4219.0

5939.0

Repeat

Note=Spectrin 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6443_6547

4219.0

5939.0

Repeat

Note=Spectrin 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6496_6519

4219.0

5939.0

Repeat

Note=LRR 24

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6552_6658

4219.0

5939.0

Repeat

Note=Spectrin 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6665_6766

4219.0

5939.0

Repeat

Note=Spectrin 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000289893

6771_6874

4219.0

5939.0

Repeat

Note=Spectrin 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

3883_3957

3717.0

5431.0

Repeat

Note=Spectrin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

3936_3958

3717.0

5431.0

Repeat

Note=LRR 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4000_4108

3717.0

5431.0

Repeat

Note=Spectrin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4125_4150

3717.0

5431.0

Repeat

Note=LRR 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4261_4287

3717.0

5431.0

Repeat

Note=LRR 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4466_4574

3717.0

5431.0

Repeat

Note=Spectrin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4511_4534

3717.0

5431.0

Repeat

Note=LRR 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4601_4624

3717.0

5431.0

Repeat

Note=LRR 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4769_4792

3717.0

5431.0

Repeat

Note=LRR 18

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4800_4904

3717.0

5431.0

Repeat

Note=Spectrin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

4909_5012

3717.0

5431.0

Repeat

Note=Spectrin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5051_5076

3717.0

5431.0

Repeat

Note=LRR 19

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5172_5194

3717.0

5431.0

Repeat

Note=LRR 20

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5236_5341

3717.0

5431.0

Repeat

Note=Spectrin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5281_5304

3717.0

5431.0

Repeat

Note=LRR 21

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5348_5450

3717.0

5431.0

Repeat

Note=Spectrin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5455_5557

3717.0

5431.0

Repeat

Note=Spectrin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5695_5719

3717.0

5431.0

Repeat

Note=LRR 22

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5783_5885

3717.0

5431.0

Repeat

Note=Spectrin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

5804_5828

3717.0

5431.0

Repeat

Note=LRR 23

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6005_6110

3717.0

5431.0

Repeat

Note=Spectrin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6115_6219

3717.0

5431.0

Repeat

Note=Spectrin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6225_6328

3717.0

5431.0

Repeat

Note=Spectrin 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6333_6439

3717.0

5431.0

Repeat

Note=Spectrin 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6443_6547

3717.0

5431.0

Repeat

Note=Spectrin 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6496_6519

3717.0

5431.0

Repeat

Note=LRR 24

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6552_6658

3717.0

5431.0

Repeat

Note=Spectrin 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6665_6766

3717.0

5431.0

Repeat

Note=Spectrin 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000361689

6771_6874

3717.0

5431.0

Repeat

Note=Spectrin 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5695_5719

5675.0

7389.0

Repeat

Note=LRR 22

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5783_5885

5675.0

7389.0

Repeat

Note=Spectrin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

5804_5828

5675.0

7389.0

Repeat

Note=LRR 23

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6005_6110

5675.0

7389.0

Repeat

Note=Spectrin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6115_6219

5675.0

7389.0

Repeat

Note=Spectrin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6225_6328

5675.0

7389.0

Repeat

Note=Spectrin 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6333_6439

5675.0

7389.0

Repeat

Note=Spectrin 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6443_6547

5675.0

7389.0

Repeat

Note=Spectrin 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6496_6519

5675.0

7389.0

Repeat

Note=LRR 24

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6552_6658

5675.0

7389.0

Repeat

Note=Spectrin 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6665_6766

5675.0

7389.0

Repeat

Note=Spectrin 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000372915

6771_6874

5675.0

7389.0

Repeat

Note=Spectrin 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

3883_3957

3717.0

5431.0

Repeat

Note=Spectrin 1

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

3936_3958

3717.0

5431.0

Repeat

Note=LRR 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4000_4108

3717.0

5431.0

Repeat

Note=Spectrin 2

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4125_4150

3717.0

5431.0

Repeat

Note=LRR 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4261_4287

3717.0

5431.0

Repeat

Note=LRR 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4466_4574

3717.0

5431.0

Repeat

Note=Spectrin 3

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4511_4534

3717.0

5431.0

Repeat

Note=LRR 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4601_4624

3717.0

5431.0

Repeat

Note=LRR 17

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4769_4792

3717.0

5431.0

Repeat

Note=LRR 18

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4800_4904

3717.0

5431.0

Repeat

Note=Spectrin 4

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

4909_5012

3717.0

5431.0

Repeat

Note=Spectrin 5

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5051_5076

3717.0

5431.0

Repeat

Note=LRR 19

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5172_5194

3717.0

5431.0

Repeat

Note=LRR 20

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5236_5341

3717.0

5431.0

Repeat

Note=Spectrin 6

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5281_5304

3717.0

5431.0

Repeat

Note=LRR 21

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5348_5450

3717.0

5431.0

Repeat

Note=Spectrin 7

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5455_5557

3717.0

5431.0

Repeat

Note=Spectrin 8

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5695_5719

3717.0

5431.0

Repeat

Note=LRR 22

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5783_5885

3717.0

5431.0

Repeat

Note=Spectrin 9

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

5804_5828

3717.0

5431.0

Repeat

Note=LRR 23

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6005_6110

3717.0

5431.0

Repeat

Note=Spectrin 10

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6115_6219

3717.0

5431.0

Repeat

Note=Spectrin 11

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6225_6328

3717.0

5431.0

Repeat

Note=Spectrin 12

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6333_6439

3717.0

5431.0

Repeat

Note=Spectrin 13

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6443_6547

3717.0

5431.0

Repeat

Note=Spectrin 14

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6496_6519

3717.0

5431.0

Repeat

Note=LRR 24

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6552_6658

3717.0

5431.0

Repeat

Note=Spectrin 15

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6665_6766

3717.0

5431.0

Repeat

Note=Spectrin 16

Hgene

MACF1

chr1:39896580

chr7:140487384

ENST00000545844

6771_6874

3717.0

5431.0

Repeat

Note=Spectrin 17

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

122_129

380.0

767.0

Compositional bias

Note=Poly-Ser

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

6_11

380.0

767.0

Compositional bias

Note=Poly-Gly

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

155_227

380.0

767.0

Domain

RBD

Tgene

BRAF

chr1:39896580

chr7:140487384

ENST00000288602

234_280

380.0

767.0

Zinc finger

Phorbol-ester/DAG-type

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

AA seq

Len(AA seq)

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

MACF1_pLDDT.png

BRAF_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

BRAF

YWHAB, YWHAG, YWHAQ, YWHAZ, SFN, HRAS, AKT1, MAPK3, RAP1GAP, RAF1, MRAS, RAP1A, PAK2, TERF1, CCDC88A, NEDD4L, Nedd4, MAP2K1, RNF149, KSR1, BRAP, PRKCE, RPS6KB2, HSP90AA1, BRAF, YWHAE, HSPA5, MAP2K2, HSPA1A, HSPA8, YWHAH, HSPA9, ARAF, CDC37, HSP90AB1, PHKB, LIMK1, IQGAP1, MAPK1, BAD, LIPF, MUS81, Vps4b, FBXW7, FGFR2, BRCA2, VHL, FNTA, HDAC2, PIK3CA, EGFR, PTEN, FNIP1, FNIP2, RAB3GAP1, KRAS, KIAA0141, FKBPL, ARMCX3, KCNC4, RPTOR, CYLD, NRAS, HSPA4, DNAJB6, PDCD11, PIP5K1A, DNAJC15, VANGL1, DNAJC11, FKBP5, HSPA4L, HSP90B1, GNAI2, DNAJC13, GNAS, PPP2CB, HSD11B2, DNAJB11, PLD2, RAP1B, WDR6, CPNE3, MYOF, COPA, UBLCP1, PPP1CA, RAD50, PIP4K2C, PHB, VIM, PGAM1, DNAJA1, MAP2K7, SPRY2, ALDOA, AP2B1, ATP5A1, SSB, IGF1R, JUP, PPP6C, PARP1, HSPB1, NME2, PRDX2, CCT7, RAB1A, FARSA, FASN, EPRS, TRAF2, REST, KIAA1429, NANOG, ITCH, SMURF2, WWP1, WWP2, PPP2CA, PPP2R2A, AURKA, LATS2, MAP2K3, MAP2K6, RASSF1, STK11, TERT, PEBP1, CRBN, FAR1, PSMC4, UBA52, UBB, UBC, RPS27A, HSPA6, HSP90AB3P, DSP, ATAD3A, ATAD3B, P4HB, SDF2L1, SLC25A22, SPTBN4, TMEM33, CTSB, NCL, HPX, TXNDC12, SLC25A11, NDUFA4, CTSV, FBP1, HSD17B3, ZNF189, ZNF510, KIF14, SRC, TRAP1, JTB, S100P, USP28,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MACF1
BRAF

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to MACF1-BRAF

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to MACF1-BRAF

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Tgene	BRAF	C0025202	melanoma	24	CGI;CTD_human;UNIPROT
Tgene	BRAF	C1275081	Cardio-facio-cutaneous syndrome	14	CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Tgene	BRAF	C0009402	Colorectal Carcinoma	8	CTD_human;UNIPROT
Tgene	BRAF	C0028326	Noonan Syndrome	8	CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET
Tgene	BRAF	C0238463	Papillary thyroid carcinoma	8	CTD_human;ORPHANET
Tgene	BRAF	C0040136	Thyroid Neoplasm	6	CGI;CTD_human
Tgene	BRAF	C0151468	Thyroid Gland Follicular Adenoma	6	CTD_human
Tgene	BRAF	C0175704	LEOPARD Syndrome	6	CLINGEN;GENOMICS_ENGLAND
Tgene	BRAF	C0549473	Thyroid carcinoma	6	CGI;CTD_human
Tgene	BRAF	C3150970	NOONAN SYNDROME 7	5	CTD_human;GENOMICS_ENGLAND;UNIPROT
Tgene	BRAF	C0009404	Colorectal Neoplasms	4	CTD_human
Tgene	BRAF	C3150971	LEOPARD SYNDROME 3	4	CTD_human;GENOMICS_ENGLAND;UNIPROT
Tgene	BRAF	C1519086	Pilomyxoid astrocytoma	3	ORPHANET
Tgene	BRAF	C0004565	Melanoma, B16	2	CTD_human
Tgene	BRAF	C0009075	Melanoma, Cloudman S91	2	CTD_human
Tgene	BRAF	C0018598	Melanoma, Harding-Passey	2	CTD_human
Tgene	BRAF	C0023443	Hairy Cell Leukemia	2	CGI;ORPHANET
Tgene	BRAF	C0025205	Melanoma, Experimental	2	CTD_human
Tgene	BRAF	C0033578	Prostatic Neoplasms	2	CTD_human
Tgene	BRAF	C0152013	Adenocarcinoma of lung (disorder)	2	CGI;CTD_human
Tgene	BRAF	C0376358	Malignant neoplasm of prostate	2	CTD_human
Tgene	BRAF	C0587248	Costello syndrome (disorder)	2	CLINGEN;CTD_human
Tgene	BRAF	C3501843	Nonmedullary Thyroid Carcinoma	2	CTD_human
Tgene	BRAF	C3501844	Familial Nonmedullary Thyroid Cancer	2	CTD_human
Tgene	BRAF	C0002448	Ameloblastoma	1	CTD_human
Tgene	BRAF	C0004114	Astrocytoma	1	CTD_human
Tgene	BRAF	C0010276	Craniopharyngioma	1	CTD_human;ORPHANET
Tgene	BRAF	C0011860	Diabetes Mellitus, Non-Insulin-Dependent	1	CTD_human
Tgene	BRAF	C0017638	Glioma	1	CGI;CTD_human
Tgene	BRAF	C0019621	Histiocytosis, Langerhans-Cell	1	CGI;ORPHANET
Tgene	BRAF	C0022665	Kidney Neoplasm	1	CTD_human
Tgene	BRAF	C0023903	Liver neoplasms	1	CTD_human
Tgene	BRAF	C0024232	Lymphatic Metastasis	1	CTD_human
Tgene	BRAF	C0024694	Mandibular Neoplasms	1	CTD_human
Tgene	BRAF	C0027659	Neoplasms, Experimental	1	CTD_human
Tgene	BRAF	C0027962	Melanocytic nevus	1	GENOMICS_ENGLAND
Tgene	BRAF	C0036920	Sezary Syndrome	1	CTD_human
Tgene	BRAF	C0041409	Turner Syndrome, Male	1	CTD_human
Tgene	BRAF	C0079773	Lymphoma, T-Cell, Cutaneous	1	CTD_human
Tgene	BRAF	C0205768	Subependymal Giant Cell Astrocytoma	1	CTD_human
Tgene	BRAF	C0206686	Adrenocortical carcinoma	1	CTD_human
Tgene	BRAF	C0206754	Neuroendocrine Tumors	1	CTD_human
Tgene	BRAF	C0259783	mixed gliomas	1	CTD_human
Tgene	BRAF	C0278875	Adult Craniopharyngioma	1	CTD_human
Tgene	BRAF	C0280783	Juvenile Pilocytic Astrocytoma	1	CTD_human
Tgene	BRAF	C0280785	Diffuse Astrocytoma	1	CTD_human
Tgene	BRAF	C0334579	Anaplastic astrocytoma	1	CGI;CTD_human
Tgene	BRAF	C0334580	Protoplasmic astrocytoma	1	CTD_human
Tgene	BRAF	C0334581	Gemistocytic astrocytoma	1	CTD_human
Tgene	BRAF	C0334582	Fibrillary Astrocytoma	1	CTD_human
Tgene	BRAF	C0334583	Pilocytic Astrocytoma	1	CGI;CTD_human
Tgene	BRAF	C0338070	Childhood Cerebral Astrocytoma	1	CTD_human
Tgene	BRAF	C0345904	Malignant neoplasm of liver	1	CTD_human
Tgene	BRAF	C0376407	Granulomatous Slack Skin	1	CTD_human
Tgene	BRAF	C0406803	Syringocystadenoma Papilliferum	1	GENOMICS_ENGLAND
Tgene	BRAF	C0431128	Papillary craniopharyngioma	1	CTD_human
Tgene	BRAF	C0431129	Adamantinous Craniopharyngioma	1	CTD_human
Tgene	BRAF	C0547065	Mixed oligoastrocytoma	1	CTD_human
Tgene	BRAF	C0555198	Malignant Glioma	1	CTD_human
Tgene	BRAF	C0596263	Carcinogenesis	1	CTD_human
Tgene	BRAF	C0684249	Carcinoma of lung	1	CGI;UNIPROT
Tgene	BRAF	C0740457	Malignant neoplasm of kidney	1	CTD_human
Tgene	BRAF	C0750935	Cerebral Astrocytoma	1	CTD_human
Tgene	BRAF	C0750936	Intracranial Astrocytoma	1	CTD_human
Tgene	BRAF	C0751061	Craniopharyngioma, Child	1	CTD_human
Tgene	BRAF	C0920269	Microsatellite Instability	1	CTD_human
Tgene	BRAF	C1527404	Female Pseudo-Turner Syndrome	1	CTD_human
Tgene	BRAF	C1704230	Grade I Astrocytoma	1	CTD_human
Tgene	BRAF	C1721098	Replication Error Phenotype	1	CTD_human
Tgene	BRAF	C2239176	Liver carcinoma	1	CTD_human
Tgene	BRAF	C4551484	Leopard Syndrome 1	1	GENOMICS_ENGLAND
Tgene	BRAF	C4551602	Noonan Syndrome 1	1	CTD_human
Tgene	BRAF	C4721532	Lymphoma, Non-Hodgkin, Familial	1	UNIPROT
Tgene	BRAF	C4733333	familial non-medullary thyroid cancer	1	GENOMICS_ENGLAND