UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine level2
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein Structure

leaf

pLDDT scores

leaf

Ramachandran Plot of Fusion Protein Structure

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:PIK3CB-SEMA5B

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: PIK3CB-SEMA5B
FusionPDB ID: 65380
FusionGDB2.0 ID: 65380
HgeneTgene
Gene symbol

PIK3CB

SEMA5B

Gene ID

5291

54437

Gene namephosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit betasemaphorin 5B
SynonymsP110BETA|PI3K|PI3KBETA|PIK3C1SEMAG|SemG
Cytomap

3q22.3

3q21.1

Type of geneprotein-codingprotein-coding
Descriptionphosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoformPI3-kinase p110 subunit betaPI3-kinase subunit betaPI3K-betaPtdIns-3-kinase p110phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit betaphosphoinositidsemaphorin-5Bsema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Modification date2020031320200313
UniProtAcc..
Ensembl transtripts involved in fusion geneENST idsENST00000289153, ENST00000477593, 
ENST00000544716, 		ENST00000465147, 
ENST00000195173, ENST00000357599, 
ENST00000451055, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score15 X 13 X 11=21454 X 4 X 3=48
# samples 176
** MAII scorelog2(17/2145*10)=-3.65737099624921
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(6/48*10)=0.321928094887362
effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs).
DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)

PubMed: PIK3CB [Title/Abstract] AND SEMA5B [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)PIK3CB(138452203)-SEMA5B(122667556), # samples:3
Anticipated loss of major functional domain due to fusion event.PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
PIK3CB-SEMA5B seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgenePIK3CB

GO:0016310

phosphorylation

25327288


check buttonFusion gene breakpoints across PIK3CB (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across SEMA5B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4KIRPTCGA-G7-6793-01APIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-
ChimerDB4KIRPTCGA-G7-6793PIK3CBchr3

138452202

-SEMA5Bchr3

122667556

-
ChimerDB4KIRPTCGA-G7-6793PIK3CBchr3

138452203

-SEMA5Bchr3

122667556

-


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000477593PIK3CBchr3138452203-ENST00000357599SEMA5Bchr3122667556-54051124113844551105
ENST00000477593PIK3CBchr3138452203-ENST00000195173SEMA5Bchr3122667556-55281124113842781046
ENST00000477593PIK3CBchr3138452203-ENST00000451055SEMA5Bchr3122667556-49351124113844551105
ENST00000477593PIK3CBchr3138452202-ENST00000357599SEMA5Bchr3122667556-54051124113844551105
ENST00000477593PIK3CBchr3138452202-ENST00000195173SEMA5Bchr3122667556-55281124113842781046
ENST00000477593PIK3CBchr3138452202-ENST00000451055SEMA5Bchr3122667556-49351124113844551105

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000477593ENST00000357599PIK3CBchr3138452203-SEMA5Bchr3122667556-0.0154933380.98450667
ENST00000477593ENST00000195173PIK3CBchr3138452203-SEMA5Bchr3122667556-0.0171793570.98282063
ENST00000477593ENST00000451055PIK3CBchr3138452203-SEMA5Bchr3122667556-0.016528680.98347133
ENST00000477593ENST00000357599PIK3CBchr3138452202-SEMA5Bchr3122667556-0.0154933380.98450667
ENST00000477593ENST00000195173PIK3CBchr3138452202-SEMA5Bchr3122667556-0.0171793570.98282063
ENST00000477593ENST00000451055PIK3CBchr3138452202-SEMA5Bchr3122667556-0.016528680.98347133

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>65380_65380_1_PIK3CB-SEMA5B_PIK3CB_chr3_138452202_ENST00000477593_SEMA5B_chr3_122667556_ENST00000195173_length(amino acids)=1046AA_BP=
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGEFKTCNP
EGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWA
AWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGED
ICLGLHTEEALCATQACPGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGKRNRTYL

--------------------------------------------------------------

>65380_65380_2_PIK3CB-SEMA5B_PIK3CB_chr3_138452202_ENST00000477593_SEMA5B_chr3_122667556_ENST00000357599_length(amino acids)=1105AA_BP=
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------

>65380_65380_3_PIK3CB-SEMA5B_PIK3CB_chr3_138452202_ENST00000477593_SEMA5B_chr3_122667556_ENST00000451055_length(amino acids)=1105AA_BP=
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------

>65380_65380_4_PIK3CB-SEMA5B_PIK3CB_chr3_138452203_ENST00000477593_SEMA5B_chr3_122667556_ENST00000195173_length(amino acids)=1046AA_BP=
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGEFKTCNP
EGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGWA
AWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGED
ICLGLHTEEALCATQACPGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGKRNRTYL

--------------------------------------------------------------

>65380_65380_5_PIK3CB-SEMA5B_PIK3CB_chr3_138452203_ENST00000477593_SEMA5B_chr3_122667556_ENST00000357599_length(amino acids)=1105AA_BP=
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------

>65380_65380_6_PIK3CB-SEMA5B_PIK3CB_chr3_138452203_ENST00000477593_SEMA5B_chr3_122667556_ENST00000451055_length(amino acids)=1105AA_BP=
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr3:138452203/chr3:122667556)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
..
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-62226_115350.01071.0DomainPI3K-ABD
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-72326_115350.01071.0DomainPI3K-ABD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-62226_115350.01071.0DomainPI3K-ABD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723194_285350.01071.0DomainPI3K-RBD
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-72326_115350.01071.0DomainPI3K-ABD
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123103_55341.3333333333333361152.0DomainSema
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123664_72041.3333333333333361152.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123722_77141.3333333333333361152.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123853_90841.3333333333333361152.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123910_96541.3333333333333361152.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000357599123966_101041.3333333333333361152.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123103_55395.333333333333331206.0DomainSema
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123664_72095.333333333333331206.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123722_77195.333333333333331206.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123853_90895.333333333333331206.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123910_96595.333333333333331206.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452202chr3:122667556ENST00000451055123966_101095.333333333333331206.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123103_55341.3333333333333361152.0DomainSema
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123664_72041.3333333333333361152.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123722_77141.3333333333333361152.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123853_90841.3333333333333361152.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123910_96541.3333333333333361152.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000357599123966_101041.3333333333333361152.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123103_55395.333333333333331206.0DomainSema
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123664_72095.333333333333331206.0DomainTSP type-1 1
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123722_77195.333333333333331206.0DomainTSP type-1 2
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123853_90895.333333333333331206.0DomainTSP type-1 3
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123910_96595.333333333333331206.0DomainTSP type-1 4
TgeneSEMA5Bchr3:138452203chr3:122667556ENST00000451055123966_101095.333333333333331206.0DomainTSP type-1 5
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000003575991231058_115141.3333333333333361152.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000004510551231058_115195.333333333333331206.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000003575991231058_115141.3333333333333361152.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000004510551231058_115195.333333333333331206.0Topological domainCytoplasmic
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000003575991231037_105741.3333333333333361152.0TransmembraneHelical%3B Signal-anchor for type III membrane protein
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000004510551231037_105795.333333333333331206.0TransmembraneHelical%3B Signal-anchor for type III membrane protein
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000003575991231037_105741.3333333333333361152.0TransmembraneHelical%3B Signal-anchor for type III membrane protein
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000004510551231037_105795.333333333333331206.0TransmembraneHelical%3B Signal-anchor for type III membrane protein

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723327_496350.01071.0DomainC2 PI3K-type
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723524_701350.01071.0DomainPIK helical
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723800_1067350.01071.0DomainPI3K/PI4K
HgenePIK3CBchr3:138452202chr3:122667556ENST00000289153-622410_418350.01071.0MotifNote=Nuclear localization signal
HgenePIK3CBchr3:138452202chr3:122667556ENST00000477593-723410_418350.01071.0MotifNote=Nuclear localization signal
HgenePIK3CBchr3:138452203chr3:122667556ENST00000289153-622410_418350.01071.0MotifNote=Nuclear localization signal
HgenePIK3CBchr3:138452203chr3:122667556ENST00000477593-723410_418350.01071.0MotifNote=Nuclear localization signal
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000003575991231_103641.3333333333333361152.0Topological domainExtracellular
TgeneSEMA5Bchr3:138452202chr3:122667556ENST000004510551231_103695.333333333333331206.0Topological domainExtracellular
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000003575991231_103641.3333333333333361152.0Topological domainExtracellular
TgeneSEMA5Bchr3:138452203chr3:122667556ENST000004510551231_103695.333333333333331206.0Topological domainExtracellular


Top

Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file >>>1867_PIK3CB_138452203_SEMA5B_122667556_ranked_0.pdbPIK3CB138452202138452203ENST00000451055SEMA5Bchr3122667556-
MPPGARTAEGPIMVLAGPLAVSLLLPSLTLLVSHLSSSQDVSSEPSSEQQLCALSKHPTVAFEDLQPWVSNFTYPGARDFSQLALDPSGN
QLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVAGRKVFMCGTNAFSPMCTSRQVGNLSRTIEKINGV
ARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGSGPPLRTAQYNSKWLNEPNFVAAYDIGLFAYFFLRENAVEHDCGRTVYS
RVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISQAFNGPFRYQ
ENPRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDLVQAKDTLYHVLYIGTESG
TILKALSTASRSLHGCYLEELHVLPPGRREPLRSLRILHSARALFVGLRDGVLRVPLERCAAYRSQGACLGARDPYCGWDGKQQRCSTLE
DSSNMSLWTQNITACPVRNVTRDGGFGPWSPWQPCEHLDGDNSGSCLCRARSCDSPRPRCGGLDCLGPAIHIANCSRNGAWTPWSSWALC
STSCGIGFQVRQRSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCGGGMQSRRRACENGNSCLGCGVEFKTCN
PEGCPEVRRNTPWTPWLPVNVTQGGARQEQRFRFTCRAPLADPHGLQFGRRRTETRTCPADGSGSCDTDALVEVLLRSGSTSPHTVSGGW
AAWGPWSSCSRDCELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTSWSPCSASCGGGHYQRTRSCTSPAPSPGE
DICLGLHTEEALCATQACPEGWSPWSEWSKCTDDGAQSRSRHCEELLPGSSACAGNSSQSRPCPYSEIPVILPASSMEEATDCAGFNLIH
LVATGISCFLGSGLLTLAVYLSCQHCQRQSQESTLVHPATPNHLHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTT
1105


Top

pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
PIK3CB_pLDDT.png
all structure
all structure
SEMA5B_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
all structure


Top

Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
PIK3CB
SEMA5B


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


Top

Related Drugs to PIK3CB-SEMA5B


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to PIK3CB-SEMA5B


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource