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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:RNF130-MGAT4B

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: RNF130-MGAT4B
	FusionPDB ID: 74774
	FusionGDB2.0 ID: 74774
		Hgene	Tgene
	Gene symbol	RNF130	MGAT4B
	Gene ID	55819	11282
	Gene name	ring finger protein 130	alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B
	Synonyms	G1RZFP\|GOLIATH\|GP	GNT-IV\|GNT-IVB
	Cytomap	5q35.3	5q35.3
	Type of gene	protein-coding	protein-coding
	Description	E3 ubiquitin-protein ligase RNF130RING-type E3 ubiquitin transferase RNF130g1-related zinc finger proteingoliath homolog	alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase BN-acetylglucosaminyltransferase IVbN-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase IVbUDP-N-acetylglucosamine: alpha-1,3-D-mannoside beta-1,4-N-acetylglucos
	Modification date	20200322	20200313
	UniProtAcc	Q86XS8	Q9UQ53
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000261947, ENST00000521389, ENST00000522208, ENST00000520564,	ENST00000521305, ENST00000292591, ENST00000337755,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	8 X 6 X 8=384	5 X 4 X 4=80
	# samples	9	6
	** MAII score	log2(9/384*10)=-2.09310940439148 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(6/80*10)=-0.415037499278844 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: RNF130 [Title/Abstract] AND MGAT4B [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MGAT4B(179225165)-RNF130(179347426), # samples:3 RNF130(179467453)-MGAT4B(179226636), # samples:2 RNF130(179467452)-MGAT4B(179226636), # samples:2
Anticipated loss of major functional domain due to fusion event.	RNF130-MGAT4B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. RNF130-MGAT4B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MGAT4B-RNF130 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across RNF130 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across MGAT4B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	READ	TCGA-F5-6814-01A	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-
ChimerDB4	READ	TCGA-F5-6814	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-
ChimerDB4	READ	TCGA-F5-6814	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000522208	RNF130	chr5	179467453	-	ENST00000337755	MGAT4B	chr5	179226636	-	1646	461	19	1197	392
ENST00000522208	RNF130	chr5	179467453	-	ENST00000292591	MGAT4B	chr5	179226636	-	1646	461	19	1197	392
ENST00000521389	RNF130	chr5	179467453	-	ENST00000337755	MGAT4B	chr5	179226636	-	2043	858	416	1594	392
ENST00000521389	RNF130	chr5	179467453	-	ENST00000292591	MGAT4B	chr5	179226636	-	2043	858	416	1594	392
ENST00000261947	RNF130	chr5	179467453	-	ENST00000337755	MGAT4B	chr5	179226636	-	2026	841	399	1577	392
ENST00000261947	RNF130	chr5	179467453	-	ENST00000292591	MGAT4B	chr5	179226636	-	2026	841	399	1577	392
ENST00000522208	RNF130	chr5	179467452	-	ENST00000337755	MGAT4B	chr5	179226636	-	1646	461	19	1197	392
ENST00000522208	RNF130	chr5	179467452	-	ENST00000292591	MGAT4B	chr5	179226636	-	1646	461	19	1197	392
ENST00000521389	RNF130	chr5	179467452	-	ENST00000337755	MGAT4B	chr5	179226636	-	2043	858	416	1594	392
ENST00000521389	RNF130	chr5	179467452	-	ENST00000292591	MGAT4B	chr5	179226636	-	2043	858	416	1594	392
ENST00000261947	RNF130	chr5	179467452	-	ENST00000337755	MGAT4B	chr5	179226636	-	2026	841	399	1577	392
ENST00000261947	RNF130	chr5	179467452	-	ENST00000292591	MGAT4B	chr5	179226636	-	2026	841	399	1577	392

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000522208	ENST00000337755	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-	0.00421763	0.9957824
ENST00000522208	ENST00000292591	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-	0.00421763	0.9957824
ENST00000521389	ENST00000337755	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-	0.00419152	0.9958085
ENST00000521389	ENST00000292591	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-	0.00419152	0.9958085
ENST00000261947	ENST00000337755	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-	0.004154332	0.99584574
ENST00000261947	ENST00000292591	RNF130	chr5	179467453	-	MGAT4B	chr5	179226636	-	0.004154332	0.99584574
ENST00000522208	ENST00000337755	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-	0.00421763	0.9957824
ENST00000522208	ENST00000292591	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-	0.00421763	0.9957824
ENST00000521389	ENST00000337755	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-	0.00419152	0.9958085
ENST00000521389	ENST00000292591	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-	0.00419152	0.9958085
ENST00000261947	ENST00000337755	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-	0.004154332	0.99584574
ENST00000261947	ENST00000292591	RNF130	chr5	179467452	-	MGAT4B	chr5	179226636	-	0.004154332	0.99584574

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>74774_74774_1_RNF130-MGAT4B_RNF130_chr5_179467452_ENST00000261947_MGAT4B_chr5_179226636_ENST00000292591_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_2_RNF130-MGAT4B_RNF130_chr5_179467452_ENST00000261947_MGAT4B_chr5_179226636_ENST00000337755_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_3_RNF130-MGAT4B_RNF130_chr5_179467452_ENST00000521389_MGAT4B_chr5_179226636_ENST00000292591_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_4_RNF130-MGAT4B_RNF130_chr5_179467452_ENST00000521389_MGAT4B_chr5_179226636_ENST00000337755_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_5_RNF130-MGAT4B_RNF130_chr5_179467452_ENST00000522208_MGAT4B_chr5_179226636_ENST00000292591_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_6_RNF130-MGAT4B_RNF130_chr5_179467452_ENST00000522208_MGAT4B_chr5_179226636_ENST00000337755_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_7_RNF130-MGAT4B_RNF130_chr5_179467453_ENST00000261947_MGAT4B_chr5_179226636_ENST00000292591_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_8_RNF130-MGAT4B_RNF130_chr5_179467453_ENST00000261947_MGAT4B_chr5_179226636_ENST00000337755_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_9_RNF130-MGAT4B_RNF130_chr5_179467453_ENST00000521389_MGAT4B_chr5_179226636_ENST00000292591_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_10_RNF130-MGAT4B_RNF130_chr5_179467453_ENST00000521389_MGAT4B_chr5_179226636_ENST00000337755_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_11_RNF130-MGAT4B_RNF130_chr5_179467453_ENST00000522208_MGAT4B_chr5_179226636_ENST00000292591_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

>74774_74774_12_RNF130-MGAT4B_RNF130_chr5_179467453_ENST00000522208_MGAT4B_chr5_179226636_ENST00000337755_length(amino acids)=392AA_BP=0
MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD
PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL
WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW
AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr5:179225165/chr5:179347426)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
RNF130 Q86XS8	MGAT4B Q9UQ53
FUNCTION: May have a role during the programmed cell death of hematopoietic cells (By similarity). Acts as an E3 ubiquitin-protein ligase. {ECO:0000250, ECO:0000269\|PubMed:16549277}.	FUNCTION: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

RNF130

chr5:179467452

chr5:179226636

ENST00000521389

105_176

147.33333333333334

420.0

Domain

Note=PA

Hgene

RNF130

chr5:179467453

chr5:179226636

ENST00000521389

105_176

147.33333333333334

420.0

Domain

Note=PA

Hgene

RNF130

chr5:179467452

chr5:179226636

ENST00000521389

218_419

147.33333333333334

420.0

Topological domain

Cytoplasmic

Hgene

RNF130

chr5:179467452

chr5:179226636

ENST00000521389

28_194

147.33333333333334

420.0

Topological domain

Extracellular

Hgene

RNF130

chr5:179467453

chr5:179226636

ENST00000521389

218_419

147.33333333333334

420.0

Topological domain

Cytoplasmic

Hgene

RNF130

chr5:179467453

chr5:179226636

ENST00000521389

28_194

147.33333333333334

420.0

Topological domain

Extracellular

Hgene

RNF130

chr5:179467452

chr5:179226636

ENST00000521389

195_217

147.33333333333334

420.0

Transmembrane

Helical

Hgene

RNF130

chr5:179467453

chr5:179226636

ENST00000521389

195_217

147.33333333333334

420.0

Transmembrane

Helical

Hgene

RNF130

chr5:179467452

chr5:179226636

ENST00000521389

264_305

147.33333333333334

420.0

Zinc finger

RING-type

Hgene

RNF130

chr5:179467453

chr5:179226636

ENST00000521389

264_305

147.33333333333334

420.0

Zinc finger

RING-type

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000292591

36_83

303.3333333333333

549.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000337755

36_83

318.3333333333333

564.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000292591

36_83

303.3333333333333

549.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000337755

36_83

318.3333333333333

564.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000292591

1_7

303.3333333333333

549.0

Topological domain

Cytoplasmic

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000292591

29_548

303.3333333333333

549.0

Topological domain

Lumenal

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000337755

1_7

318.3333333333333

564.0

Topological domain

Cytoplasmic

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000337755

29_548

318.3333333333333

564.0

Topological domain

Lumenal

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000292591

1_7

303.3333333333333

549.0

Topological domain

Cytoplasmic

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000292591

29_548

303.3333333333333

549.0

Topological domain

Lumenal

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000337755

1_7

318.3333333333333

564.0

Topological domain

Cytoplasmic

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000337755

29_548

318.3333333333333

564.0

Topological domain

Lumenal

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000292591

8_28

303.3333333333333

549.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

MGAT4B

chr5:179467452

chr5:179226636

ENST00000337755

8_28

318.3333333333333

564.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000292591

8_28

303.3333333333333

549.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

Tgene

MGAT4B

chr5:179467453

chr5:179226636

ENST00000337755

8_28

318.3333333333333

564.0

Transmembrane

Helical%3B Signal-anchor for type II membrane protein

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>786_RNF130_179467453_MGAT4B_179226636_ranked_0.pdb	RNF130	179467452	179467453	ENST00000292591	MGAT4B	chr5	179226636	-	MSCAGRAGPARLAALALLTCSLWPARADNASQEYYTALINVTVQEPGRGAPLTFRIDRGRYGLDSPKAEVRGQVLAPLPLHGVADHLGCD PQTRFFVPPNIKQWIALLQRGNCTFKEKISRAAFHNAVAVVIYNNKSKEEPVTMTHPGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHIL WVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFW AFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVD	392

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

RNF130_pLDDT.png

MGAT4B_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene	PPI interactors
RNF130	FGFR3, UBE2D1, UBE2D2, UBE2D3, UBE2D4, UBE2E1, UBE2E3, UBE2N, UBE2Z, MYC, UBC, CD1B, TSPAN18, NRG1, TMEM231, MCOLN3, TSPAN17, BTNL8, PTCH1, ZACN, HTR3C, PON2, CDC5L, SCGB1D4, GGT7, MANSC1, HTR3A, HNRNPL, CSK, ORF7b, ORF3a, FASN, EDDM3B, CHRNB4, BTNL3, CLRN2, CHRNB2, PCDHGA9, PCDHGC4,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
RNF130
MGAT4B

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to RNF130-MGAT4B

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to RNF130-MGAT4B

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	RNF130	C0033578	Prostatic Neoplasms	1	CTD_human
Hgene	RNF130	C0376358	Malignant neoplasm of prostate	1	CTD_human