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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:TANC2-PRKCA

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: TANC2-PRKCA
	FusionPDB ID: 88951
	FusionGDB2.0 ID: 88951
		Hgene	Tgene
	Gene symbol	TANC2	PRKCA
	Gene ID	26115	5578
	Gene name	tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2	protein kinase C alpha
	Synonyms	ROLSA\|rols	AAG6\|PKC-alpha\|PKCA\|PKCI+/-\|PKCalpha\|PRKACA
	Cytomap	17q23.2-q23.3	17q24.2
	Type of gene	protein-coding	protein-coding
	Description	protein TANC2putative ankyrin-repeat containing proteinrolling pebbles homolog Btetratricopeptide repeat, ankyrin repeat and coiled-coil domain-containing protein 2	protein kinase C alpha typePKC-Aaging-associated gene 6
	Modification date	20200313	20200327
	UniProtAcc	.	PICK1
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000389520, ENST00000424789,	ENST00000583361, ENST00000413366,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	39 X 25 X 15=14625	26 X 21 X 9=4914
	# samples	45	29
	** MAII score	log2(45/14625*10)=-5.02236781302845 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(29/4914*10)=-4.08277305234723 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: TANC2 [Title/Abstract] AND PRKCA [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	TANC2(61151375)-PRKCA(64683229), # samples:3
Anticipated loss of major functional domain due to fusion event.	TANC2-PRKCA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TANC2-PRKCA seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TANC2-PRKCA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. TANC2-PRKCA seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	PRKCA	GO:0006468	protein phosphorylation	10770950
Tgene	PRKCA	GO:0035408	histone H3-T6 phosphorylation	20228790
Tgene	PRKCA	GO:0043536	positive regulation of blood vessel endothelial cell migration	20011604
Tgene	PRKCA	GO:0090330	regulation of platelet aggregation	12724315

Fusion gene breakpoints across TANC2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across PRKCA (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	LUSC	TCGA-43-2581-01A	TANC2	chr17	61151375	-	PRKCA	chr17	64683229	+
ChimerDB4	LUSC	TCGA-43-2581-01A	TANC2	chr17	61151375	+	PRKCA	chr17	64683229	+
ChimerDB4	LUSC	TCGA-43-2581	TANC2	chr17	61151375	+	PRKCA	chr17	64683229	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000424789

TANC2

chr17

61151375

ENST00000413366

PRKCA

chr17

64683229

8339

143

1632

542

ENST00000389520

TANC2

chr17

61151375

ENST00000413366

PRKCA

chr17

64683229

8335

139

1628

542

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000424789	ENST00000413366	TANC2	chr17	61151375	+	PRKCA	chr17	64683229	+	0.000393372	0.99960667
ENST00000389520	ENST00000413366	TANC2	chr17	61151375	+	PRKCA	chr17	64683229	+	0.000393575	0.99960643

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>88951_88951_1_TANC2-PRKCA_TANC2_chr17_61151375_ENST00000389520_PRKCA_chr17_64683229_ENST00000413366_length(amino acids)=542AA_BP=46
MFRNSLKMLLTGGKSSRKNRSSDGGSEEPPDRRQSSVDSRQSRSGQVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLN
PQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKL
GPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFL
TQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMM
DGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSVCKGLMTKHPAKR
LGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQS

--------------------------------------------------------------

>88951_88951_2_TANC2-PRKCA_TANC2_chr17_61151375_ENST00000424789_PRKCA_chr17_64683229_ENST00000413366_length(amino acids)=542AA_BP=46
MFRNSLKMLLTGGKSSRKNRSSDGGSEEPPDRRQSSVDSRQSRSGQVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLN
PQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKL
GPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFL
TQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMM
DGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSVCKGLMTKHPAKR
LGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQS

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:61151375/chr17:64683229)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	PRKCA PICK1
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	415

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

PRKCA

chr17:61151375

chr17:64683229

ENST00000413366

339_597

176.33333333333334

673.0

Domain

Protein kinase

Tgene

PRKCA

chr17:61151375

chr17:64683229

ENST00000413366

598_668

176.33333333333334

673.0

Domain

AGC-kinase C-terminal

Tgene

PRKCA

chr17:61151375

chr17:64683229

ENST00000413366

345_353

176.33333333333334

673.0

Nucleotide binding

ATP

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1033_1062

46.333333333333336

2001.0

Repeat

Note=ANK 6

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1066_1095

46.333333333333336

2001.0

Repeat

Note=ANK 7

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1099_1128

46.333333333333336

2001.0

Repeat

Note=ANK 8

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1132_1161

46.333333333333336

2001.0

Repeat

Note=ANK 9

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1165_1194

46.333333333333336

2001.0

Repeat

Note=ANK 10

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1198_1227

46.333333333333336

2001.0

Repeat

Note=ANK 11

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1244_1277

46.333333333333336

2001.0

Repeat

Note=TPR 1

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1291_1324

46.333333333333336

2001.0

Repeat

Note=TPR 2

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

1325_1358

46.333333333333336

2001.0

Repeat

Note=TPR 3

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

846_878

46.333333333333336

2001.0

Repeat

Note=ANK 1

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

884_913

46.333333333333336

2001.0

Repeat

Note=ANK 2

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

917_946

46.333333333333336

2001.0

Repeat

Note=ANK 3

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

950_979

46.333333333333336

2001.0

Repeat

Note=ANK 4

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000389520

990_1019

46.333333333333336

2001.0

Repeat

Note=ANK 5

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1033_1062

46.333333333333336

1991.0

Repeat

Note=ANK 6

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1066_1095

46.333333333333336

1991.0

Repeat

Note=ANK 7

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1099_1128

46.333333333333336

1991.0

Repeat

Note=ANK 8

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1132_1161

46.333333333333336

1991.0

Repeat

Note=ANK 9

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1165_1194

46.333333333333336

1991.0

Repeat

Note=ANK 10

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1198_1227

46.333333333333336

1991.0

Repeat

Note=ANK 11

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1244_1277

46.333333333333336

1991.0

Repeat

Note=TPR 1

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1291_1324

46.333333333333336

1991.0

Repeat

Note=TPR 2

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

1325_1358

46.333333333333336

1991.0

Repeat

Note=TPR 3

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

846_878

46.333333333333336

1991.0

Repeat

Note=ANK 1

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

884_913

46.333333333333336

1991.0

Repeat

Note=ANK 2

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

917_946

46.333333333333336

1991.0

Repeat

Note=ANK 3

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

950_979

46.333333333333336

1991.0

Repeat

Note=ANK 4

Hgene

TANC2

chr17:61151375

chr17:64683229

ENST00000424789

990_1019

46.333333333333336

1991.0

Repeat

Note=ANK 5

Tgene

PRKCA

chr17:61151375

chr17:64683229

ENST00000413366

158_275

176.33333333333334

673.0

Domain

Tgene

PRKCA

chr17:61151375

chr17:64683229

ENST00000413366

101_151

176.33333333333334

673.0

Zinc finger

Phorbol-ester/DAG-type 2

Tgene

PRKCA

chr17:61151375

chr17:64683229

ENST00000413366

36_86

176.33333333333334

673.0

Zinc finger

Phorbol-ester/DAG-type 1

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1160_TANC2_61151375_PRKCA_64683229_1160_TANC2_61151375_PRKCA_64683229_ranked_0.pdb	TANC2	61151375	61151375	ENST00000413366	PRKCA	chr17	64683229	+	MFRNSLKMLLTGGKSSRKNRSSDGGSEEPPDRRQSSVDSRQSRSGQVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIRSTLN PQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGWYKLLNQEEGEYYNVPIPEGDEEGNMELRQKFEKAKL GPAGNKVISPSEDRKQPSNNLDRVKLTDFNFLMVLGKGSFGKVMLADRKGTEELYAIKILKKDVVIQDDDVECTMVEKRVLALLDKPPFL TQLHSCFQTVDRLYFVMEYVNGGDLMYHIQQVGKFKEPQAVFYAAEISIGLFFLHKRGIIYRDLKLDNVMLDSEGHIKIADFGMCKEHMM DGVTTRTFCGTPDYIAPEIIAYQPYGKSVDWWAYGVLLYEMLAGQPPFDGEDEDELFQSIMEHNVSYPKSLSKEAVSVCKGLMTKHPAKR LGCGPEGERDVREHAFFRRIDWEKLENREIQPPFKPKVCGKGAENFDKFFTRGQPVLTPPDQLVIANIDQSDFEGFSYVNPQFVHPILQS	542

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

TANC2_pLDDT.png

PRKCA_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
TANC2
PRKCA

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to TANC2-PRKCA

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to TANC2-PRKCA

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source