UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level2

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:TBC1D10A-POLR3H

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: TBC1D10A-POLR3H
	FusionPDB ID: 89193
	FusionGDB2.0 ID: 89193
		Hgene	Tgene
	Gene symbol	TBC1D10A	POLR3H
	Gene ID	83874	171568
	Gene name	TBC1 domain family member 10A	RNA polymerase III subunit H
	Synonyms	EPI64\|TBC1D10\|dJ130H16.1\|dJ130H16.2	C25\|RPC22.9\|RPC8
	Cytomap	22q12.2	22q13.2
	Type of gene	protein-coding	protein-coding
	Description	TBC1 domain family member 10AEBP50-PDX interactor of 64 kDaEBP50-PDZ interactor of 64 kDrab27A-GAP-alpha	DNA-directed RNA polymerase III subunit RPC8DNA-directed RNA polymerase III subunit 22.9 kDa polypeptideDNA-directed RNA polymerase III subunit HRNA nucleotidyltransferase (DNA-directed)RNA polymerase III subunit C8polymerase (RNA) III (DNA directed)
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000215790, ENST00000403362, ENST00000403477, ENST00000490449,	ENST00000420561, ENST00000337566, ENST00000355209, ENST00000396504, ENST00000407461,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 6 X 5=180	7 X 8 X 5=280
	# samples	8	9
	** MAII score	log2(8/180*10)=-1.16992500144231 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/280*10)=-1.63742992061529 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: TBC1D10A [Title/Abstract] AND POLR3H [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	TBC1D10A(30695433)-POLR3H(41936799), # samples:3
Anticipated loss of major functional domain due to fusion event.	TBC1D10A-POLR3H seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TBC1D10A-POLR3H seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. TBC1D10A-POLR3H seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. TBC1D10A-POLR3H seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. TBC1D10A-POLR3H seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	TBC1D10A	GO:0045862	positive regulation of proteolysis	18387192
Hgene	TBC1D10A	GO:0097202	activation of cysteine-type endopeptidase activity	18387192
Tgene	POLR3H	GO:0006139	nucleobase-containing compound metabolic process	12391170
Tgene	POLR3H	GO:0006383	transcription by RNA polymerase III	12391170

Fusion gene breakpoints across TBC1D10A (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across POLR3H (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	HNSC	TCGA-CX-7086-01A	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-
ChimerDB4	HNSC	TCGA-CX-7086	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-
ChimerDB4	HNSC	TCGA-CX-7086	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000215790	TBC1D10A	chr22	30695433	-	ENST00000396504	POLR3H	chr22	41936799	-	4565	582	48	1085	345
ENST00000215790	TBC1D10A	chr22	30695433	-	ENST00000355209	POLR3H	chr22	41936799	-	4543	582	48	1085	345
ENST00000215790	TBC1D10A	chr22	30695433	-	ENST00000337566	POLR3H	chr22	41936799	-	1440	582	48	998	316
ENST00000215790	TBC1D10A	chr22	30695433	-	ENST00000407461	POLR3H	chr22	41936799	-	1157	582	48	1085	345
ENST00000403477	TBC1D10A	chr22	30695433	-	ENST00000396504	POLR3H	chr22	41936799	-	4441	458	20	961	313
ENST00000403477	TBC1D10A	chr22	30695433	-	ENST00000355209	POLR3H	chr22	41936799	-	4419	458	20	961	313
ENST00000403477	TBC1D10A	chr22	30695433	-	ENST00000337566	POLR3H	chr22	41936799	-	1316	458	20	874	284
ENST00000403477	TBC1D10A	chr22	30695433	-	ENST00000407461	POLR3H	chr22	41936799	-	1033	458	20	961	313
ENST00000215790	TBC1D10A	chr22	30695432	-	ENST00000396504	POLR3H	chr22	41936799	-	4565	582	48	1085	345
ENST00000215790	TBC1D10A	chr22	30695432	-	ENST00000355209	POLR3H	chr22	41936799	-	4543	582	48	1085	345
ENST00000215790	TBC1D10A	chr22	30695432	-	ENST00000337566	POLR3H	chr22	41936799	-	1440	582	48	998	316
ENST00000215790	TBC1D10A	chr22	30695432	-	ENST00000407461	POLR3H	chr22	41936799	-	1157	582	48	1085	345
ENST00000403477	TBC1D10A	chr22	30695432	-	ENST00000396504	POLR3H	chr22	41936799	-	4441	458	20	961	313
ENST00000403477	TBC1D10A	chr22	30695432	-	ENST00000355209	POLR3H	chr22	41936799	-	4419	458	20	961	313
ENST00000403477	TBC1D10A	chr22	30695432	-	ENST00000337566	POLR3H	chr22	41936799	-	1316	458	20	874	284
ENST00000403477	TBC1D10A	chr22	30695432	-	ENST00000407461	POLR3H	chr22	41936799	-	1033	458	20	961	313

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000215790	ENST00000396504	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.006869371	0.9931306
ENST00000215790	ENST00000355209	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.00694347	0.9930566
ENST00000215790	ENST00000337566	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.011576816	0.98842317
ENST00000215790	ENST00000407461	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.010699727	0.98930025
ENST00000403477	ENST00000396504	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.005712417	0.99428755
ENST00000403477	ENST00000355209	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.005764213	0.9942358
ENST00000403477	ENST00000337566	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.013430927	0.98656905
ENST00000403477	ENST00000407461	TBC1D10A	chr22	30695433	-	POLR3H	chr22	41936799	-	0.011208663	0.98879135
ENST00000215790	ENST00000396504	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.006869371	0.9931306
ENST00000215790	ENST00000355209	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.00694347	0.9930566
ENST00000215790	ENST00000337566	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.011576816	0.98842317
ENST00000215790	ENST00000407461	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.010699727	0.98930025
ENST00000403477	ENST00000396504	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.005712417	0.99428755
ENST00000403477	ENST00000355209	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.005764213	0.9942358
ENST00000403477	ENST00000337566	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.013430927	0.98656905
ENST00000403477	ENST00000407461	TBC1D10A	chr22	30695432	-	POLR3H	chr22	41936799	-	0.011208663	0.98879135

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>89193_89193_1_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000215790_POLR3H_chr22_41936799_ENST00000337566_length(amino acids)=316AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFV

--------------------------------------------------------------

>89193_89193_2_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000215790_POLR3H_chr22_41936799_ENST00000355209_length(amino acids)=345AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW

--------------------------------------------------------------

>89193_89193_3_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000215790_POLR3H_chr22_41936799_ENST00000396504_length(amino acids)=345AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW

--------------------------------------------------------------

>89193_89193_4_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000215790_POLR3H_chr22_41936799_ENST00000407461_length(amino acids)=345AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW

--------------------------------------------------------------

>89193_89193_5_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000403477_POLR3H_chr22_41936799_ENST00000337566_length(amino acids)=284AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVS
LGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLVGSI

--------------------------------------------------------------

>89193_89193_6_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000403477_POLR3H_chr22_41936799_ENST00000355209_length(amino acids)=313AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVH
FRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTS

--------------------------------------------------------------

>89193_89193_7_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000403477_POLR3H_chr22_41936799_ENST00000396504_length(amino acids)=313AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVH
FRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTS

--------------------------------------------------------------

>89193_89193_8_TBC1D10A-POLR3H_TBC1D10A_chr22_30695432_ENST00000403477_POLR3H_chr22_41936799_ENST00000407461_length(amino acids)=313AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVH
FRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTS

--------------------------------------------------------------

>89193_89193_9_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000215790_POLR3H_chr22_41936799_ENST00000337566_length(amino acids)=316AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFV

--------------------------------------------------------------

>89193_89193_10_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000215790_POLR3H_chr22_41936799_ENST00000355209_length(amino acids)=345AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW

--------------------------------------------------------------

>89193_89193_11_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000215790_POLR3H_chr22_41936799_ENST00000396504_length(amino acids)=345AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW

--------------------------------------------------------------

>89193_89193_12_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000215790_POLR3H_chr22_41936799_ENST00000407461_length(amino acids)=345AA_BP=178
MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA
ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV
YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW

--------------------------------------------------------------

>89193_89193_13_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000403477_POLR3H_chr22_41936799_ENST00000337566_length(amino acids)=284AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVS
LGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTSPTGPSSADATTSSEELPKKEAPYTLVGSI

--------------------------------------------------------------

>89193_89193_14_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000403477_POLR3H_chr22_41936799_ENST00000355209_length(amino acids)=313AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVH
FRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTS

--------------------------------------------------------------

>89193_89193_15_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000403477_POLR3H_chr22_41936799_ENST00000396504_length(amino acids)=313AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVH
FRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTS

--------------------------------------------------------------

>89193_89193_16_TBC1D10A-POLR3H_TBC1D10A_chr22_30695433_ENST00000403477_POLR3H_chr22_41936799_ENST00000407461_length(amino acids)=313AA_BP=146
MAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFAERRIDKFGFIVGSQGAEGAPCPLLHRLEEVPLEVLRQRE
SKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVVYNVGLCICLFDITKLEDAYVFPGDGASHTKVH
FRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVWEYETEEGAHDLYMDTGEEIRFRVVDESFVDTS

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:30695433/chr22:41936799)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

TBC1D10A

chr22:30695432

chr22:41936799

ENST00000215790

111_299

139.0

509.0

Domain

Rab-GAP TBC

Hgene

TBC1D10A

chr22:30695432

chr22:41936799

ENST00000403477

111_299

146.0

516.0

Domain

Rab-GAP TBC

Hgene

TBC1D10A

chr22:30695433

chr22:41936799

ENST00000215790

111_299

139.0

509.0

Domain

Rab-GAP TBC

Hgene

TBC1D10A

chr22:30695433

chr22:41936799

ENST00000403477

111_299

146.0

516.0

Domain

Rab-GAP TBC

Hgene

TBC1D10A

chr22:30695432

chr22:41936799

ENST00000215790

505_508

139.0

509.0

Region

Note=Binding to the PDZ domain of EBP50

Hgene

TBC1D10A

chr22:30695432

chr22:41936799

ENST00000403477

505_508

146.0

516.0

Region

Note=Binding to the PDZ domain of EBP50

Hgene

TBC1D10A

chr22:30695433

chr22:41936799

ENST00000215790

505_508

139.0

509.0

Region

Note=Binding to the PDZ domain of EBP50

Hgene

TBC1D10A

chr22:30695433

chr22:41936799

ENST00000403477

505_508

146.0

516.0

Region

Note=Binding to the PDZ domain of EBP50

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>662_TBC1D10A_30695433_POLR3H_41936799_ranked_0.pdb	TBC1D10A	30695432	30695433	ENST00000407461	POLR3H	chr22	41936799	-	MEPRPPALRRPIGAARGRGVGGSRAAEVAAGAQAAGAAGMAKSNGENGPRAPAAGESLSGTRESLAQGPDAATTDELSSLGSDSEANGFA ERRIDKFGFIVGSQGAEGALEEVPLEVLRQRESKWLDMLNNWDKWMAKKHKKIRLRCQKGIPPSLRGRAWQYLSGGKVKLQQNPGKFDVV YNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFDEAEQVWVW	345

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

TBC1D10A_pLDDT.png

POLR3H_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
TBC1D10A
POLR3H

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to TBC1D10A-POLR3H

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to TBC1D10A-POLR3H

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source