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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:BRAF-AGK

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: BRAF-AGK
	FusionPDB ID: 10111
	FusionGDB2.0 ID: 10111
		Hgene	Tgene
	Gene symbol	BRAF	AGK
	Gene ID	673	55750
	Gene name	B-Raf proto-oncogene, serine/threonine kinase	acylglycerol kinase
	Synonyms	B-RAF1\|B-raf\|BRAF1\|NS7\|RAFB1	CATC5\|CTRCT38\|MTDPS10\|MULK
	Cytomap	7q34	7q34
	Type of gene	protein-coding	protein-coding
	Description	serine/threonine-protein kinase B-raf94 kDa B-raf proteinB-Raf proto-oncogene serine/threonine-protein kinase (p94)B-Raf serine/threonine-proteinmurine sarcoma viral (v-raf) oncogene homolog B1proto-oncogene B-Rafv-raf murine sarcoma viral oncogene	acylglycerol kinase, mitochondrialhAGKhsMuLKmulti-substrate lipid kinasemultiple substrate lipid kinase
	Modification date	20200329	20200313
	UniProtAcc	P15056	Q53H12
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000288602,	ENST00000492693, ENST00000495028, ENST00000535825, ENST00000355413, ENST00000473247,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	28 X 21 X 11=6468	7 X 8 X 3=168
	# samples	32	7
	** MAII score	log2(32/6468*10)=-4.3371758685863 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/168*10)=-1.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: BRAF [Title/Abstract] AND AGK [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	BRAF(140500162)-AGK(141292946), # samples:4 AGK(141255367)-BRAF(140494267), # samples:3
Anticipated loss of major functional domain due to fusion event.	AGK-BRAF seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. AGK-BRAF seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. BRAF-AGK seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BRAF-AGK seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. BRAF-AGK seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. BRAF-AGK seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. AGK-BRAF seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. AGK-BRAF seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. AGK-BRAF seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. AGK-BRAF seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. AGK-BRAF seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	BRAF	GO:0000186	activation of MAPKK activity	29433126
Hgene	BRAF	GO:0006468	protein phosphorylation	17563371
Hgene	BRAF	GO:0010828	positive regulation of glucose transmembrane transport	23010278
Hgene	BRAF	GO:0033138	positive regulation of peptidyl-serine phosphorylation	19667065
Hgene	BRAF	GO:0043066	negative regulation of apoptotic process	19667065
Hgene	BRAF	GO:0070374	positive regulation of ERK1 and ERK2 cascade	22065586
Hgene	BRAF	GO:0071277	cellular response to calcium ion	18567582
Hgene	BRAF	GO:0090150	establishment of protein localization to membrane	23010278
Tgene	AGK	GO:0045039	protein import into mitochondrial inner membrane	28712724\|28712726

Fusion gene breakpoints across BRAF (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across AGK (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	SKCM	TCGA-DA-A1IA-06A	BRAF	chr7	140500162	-	AGK	chr7	141292946	+
ChimerDB4	SKCM	TCGA-DA-A1IA	BRAF	chr7	140500161	-	AGK	chr7	141292945	+
ChimerDB4	THCA	TCGA-DJ-A2PX-01A	BRAF	chr7	140500162	-	AGK	chr7	141292946	+
ChimerDB4	THCA	TCGA-DJ-A2PX	BRAF	chr7	140500161	-	AGK	chr7	141292945	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000288602	BRAF	chr7	140500161	-	ENST00000355413	AGK	chr7	141292945	+	4529	1041	61	2208	715
ENST00000288602	BRAF	chr7	140500161	-	ENST00000473247	AGK	chr7	141292945	+	2432	1041	61	2208	715
ENST00000288602	BRAF	chr7	140500162	-	ENST00000355413	AGK	chr7	141292946	+	4529	1041	61	2208	715
ENST00000288602	BRAF	chr7	140500162	-	ENST00000473247	AGK	chr7	141292946	+	2432	1041	61	2208	715

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000288602	ENST00000355413	BRAF	chr7	140500161	-	AGK	chr7	141292945	+	0.000513829	0.9994861
ENST00000288602	ENST00000473247	BRAF	chr7	140500161	-	AGK	chr7	141292945	+	0.001537427	0.99846256
ENST00000288602	ENST00000355413	BRAF	chr7	140500162	-	AGK	chr7	141292946	+	0.000513829	0.9994861
ENST00000288602	ENST00000473247	BRAF	chr7	140500162	-	AGK	chr7	141292946	+	0.001537427	0.99846256

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>10111_10111_1_BRAF-AGK_BRAF_chr7_140500161_ENST00000288602_AGK_chr7_141292945_ENST00000355413_length(amino acids)=715AA_BP=327
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLD
ALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS
LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQ
RCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFL
NPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGET
SSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQT
HQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEP

--------------------------------------------------------------

>10111_10111_2_BRAF-AGK_BRAF_chr7_140500161_ENST00000288602_AGK_chr7_141292945_ENST00000473247_length(amino acids)=715AA_BP=327
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLD
ALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS
LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQ
RCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFL
NPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGET
SSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQT
HQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEP

--------------------------------------------------------------

>10111_10111_3_BRAF-AGK_BRAF_chr7_140500162_ENST00000288602_AGK_chr7_141292946_ENST00000355413_length(amino acids)=715AA_BP=327
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLD
ALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS
LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQ
RCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFL
NPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGET
SSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQT
HQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEP

--------------------------------------------------------------

>10111_10111_4_BRAF-AGK_BRAF_chr7_140500162_ENST00000288602_AGK_chr7_141292946_ENST00000473247_length(amino acids)=715AA_BP=327
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLD
ALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS
LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQ
RCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFL
NPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGET
SSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQT
HQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEP

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:140500162/chr7:141292946)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
BRAF P15056	AGK Q53H12
FUNCTION: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179). {ECO:0000269\|PubMed:1508179, ECO:0000269\|PubMed:21441910, ECO:0000269\|PubMed:29433126, ECO:0000305}.	FUNCTION: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15939762). Does not phosphorylate sphingosine (PubMed:15939762). Phosphorylates ceramide (By similarity). Phosphorylates 1,2-dioleoylglycerol more rapidly than 2,3-dioleoylglycerol (By similarity). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (PubMed:28712724, PubMed:28712726). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (PubMed:28712724, PubMed:28712726). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (PubMed:28712724). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (PubMed:15939762). {ECO:0000250\|UniProtKB:Q9ESW4, ECO:0000269\|PubMed:15939762, ECO:0000269\|PubMed:28712724, ECO:0000269\|PubMed:28712726}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

122_129

326.6666666666667

767.0

Compositional bias

Note=Poly-Ser

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

6_11

326.6666666666667

767.0

Compositional bias

Note=Poly-Gly

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

122_129

326.6666666666667

767.0

Compositional bias

Note=Poly-Ser

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

6_11

326.6666666666667

767.0

Compositional bias

Note=Poly-Gly

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

155_227

326.6666666666667

767.0

Domain

RBD

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

155_227

326.6666666666667

767.0

Domain

RBD

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

234_280

326.6666666666667

767.0

Zinc finger

Phorbol-ester/DAG-type

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

234_280

326.6666666666667

767.0

Zinc finger

Phorbol-ester/DAG-type

Tgene

AGK

chr7:140500161

chr7:141292945

ENST00000355413

58_199

33.666666666666664

423.0

Domain

DAGKc

Tgene

AGK

chr7:140500161

chr7:141292945

ENST00000492693

58_199

33.666666666666664

66.0

Domain

DAGKc

Tgene

AGK

chr7:140500162

chr7:141292946

ENST00000355413

58_199

33.666666666666664

423.0

Domain

DAGKc

Tgene

AGK

chr7:140500162

chr7:141292946

ENST00000492693

58_199

33.666666666666664

66.0

Domain

DAGKc

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

428_432

326.6666666666667

767.0

Compositional bias

Note=Poly-Ser

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

428_432

326.6666666666667

767.0

Compositional bias

Note=Poly-Ser

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

457_717

326.6666666666667

767.0

Domain

Protein kinase

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

457_717

326.6666666666667

767.0

Domain

Protein kinase

Hgene

BRAF

chr7:140500161

chr7:141292945

ENST00000288602

463_471

326.6666666666667

767.0

Nucleotide binding

ATP

Hgene

BRAF

chr7:140500162

chr7:141292946

ENST00000288602

463_471

326.6666666666667

767.0

Nucleotide binding

ATP

Tgene

AGK

chr7:140500161

chr7:141292945

ENST00000355413

15_31

33.666666666666664

423.0

Region

Hydrophobic

Tgene

AGK

chr7:140500161

chr7:141292945

ENST00000492693

15_31

33.666666666666664

66.0

Region

Hydrophobic

Tgene

AGK

chr7:140500162

chr7:141292946

ENST00000355413

15_31

33.666666666666664

423.0

Region

Hydrophobic

Tgene

AGK

chr7:140500162

chr7:141292946

ENST00000492693

15_31

33.666666666666664

66.0

Region

Hydrophobic

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1457_BRAF_140500161_AGK_141292945_ranked_0.pdb	BRAF	140500162	140500161	ENST00000473247	AGK	chr7	141292945	+	MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLD ALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDS LKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQ RCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGDNLLRRAACQEAQVFGNQLIPPNAQVKKATVFL NPAACKGKARTLFEKNAAPILHLSGMDVTIVKTDYEGQAKKLLELMENTDVIIVAGGDGTLQEVVTGVLRRTDEATFSKIPIGFIPLGET SSLSHTLFAESGNKVQHITDATLAIVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQT HQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQPQDALSQEVSPEVWKDVQLSTIELSITTRNNQLDPTSKEDFLNICIEP	715

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

BRAF_pLDDT.png

AGK_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

BRAF

YWHAB, YWHAG, YWHAQ, YWHAZ, SFN, HRAS, AKT1, MAPK3, RAP1GAP, RAF1, MRAS, RAP1A, PAK2, TERF1, CCDC88A, NEDD4L, Nedd4, MAP2K1, RNF149, KSR1, BRAP, PRKCE, RPS6KB2, HSP90AA1, BRAF, YWHAE, HSPA5, MAP2K2, HSPA1A, HSPA8, YWHAH, HSPA9, ARAF, CDC37, HSP90AB1, PHKB, LIMK1, IQGAP1, MAPK1, BAD, LIPF, MUS81, Vps4b, FBXW7, FGFR2, BRCA2, VHL, FNTA, HDAC2, PIK3CA, EGFR, PTEN, FNIP1, FNIP2, RAB3GAP1, KRAS, KIAA0141, FKBPL, ARMCX3, KCNC4, RPTOR, CYLD, NRAS, HSPA4, DNAJB6, PDCD11, PIP5K1A, DNAJC15, VANGL1, DNAJC11, FKBP5, HSPA4L, HSP90B1, GNAI2, DNAJC13, GNAS, PPP2CB, HSD11B2, DNAJB11, PLD2, RAP1B, WDR6, CPNE3, MYOF, COPA, UBLCP1, PPP1CA, RAD50, PIP4K2C, PHB, VIM, PGAM1, DNAJA1, MAP2K7, SPRY2, ALDOA, AP2B1, ATP5A1, SSB, IGF1R, JUP, PPP6C, PARP1, HSPB1, NME2, PRDX2, CCT7, RAB1A, FARSA, FASN, EPRS, TRAF2, REST, KIAA1429, NANOG, ITCH, SMURF2, WWP1, WWP2, PPP2CA, PPP2R2A, AURKA, LATS2, MAP2K3, MAP2K6, RASSF1, STK11, TERT, PEBP1, CRBN, FAR1, PSMC4, UBA52, UBB, UBC, RPS27A, HSPA6, HSP90AB3P, DSP, ATAD3A, ATAD3B, P4HB, SDF2L1, SLC25A22, SPTBN4, TMEM33, CTSB, NCL, HPX, TXNDC12, SLC25A11, NDUFA4, CTSV, FBP1, HSD17B3, ZNF189, ZNF510, KIF14, SRC, TRAP1, JTB, S100P, USP28,

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
BRAF
AGK

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to BRAF-AGK

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to BRAF-AGK

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner	Gene	Disease ID	Disease name	# pubmeds	Source
Hgene	BRAF	C0025202	melanoma	24	CGI;CTD_human;UNIPROT
Hgene	BRAF	C1275081	Cardio-facio-cutaneous syndrome	14	CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
Hgene	BRAF	C0009402	Colorectal Carcinoma	8	CTD_human;UNIPROT
Hgene	BRAF	C0028326	Noonan Syndrome	8	CLINGEN;CTD_human;GENOMICS_ENGLAND;ORPHANET
Hgene	BRAF	C0238463	Papillary thyroid carcinoma	8	CTD_human;ORPHANET
Hgene	BRAF	C0040136	Thyroid Neoplasm	6	CGI;CTD_human
Hgene	BRAF	C0151468	Thyroid Gland Follicular Adenoma	6	CTD_human
Hgene	BRAF	C0175704	LEOPARD Syndrome	6	CLINGEN;GENOMICS_ENGLAND
Hgene	BRAF	C0549473	Thyroid carcinoma	6	CGI;CTD_human
Hgene	BRAF	C3150970	NOONAN SYNDROME 7	5	CTD_human;GENOMICS_ENGLAND;UNIPROT
Hgene	BRAF	C0009404	Colorectal Neoplasms	4	CTD_human
Hgene	BRAF	C3150971	LEOPARD SYNDROME 3	4	CTD_human;GENOMICS_ENGLAND;UNIPROT
Hgene	BRAF	C1519086	Pilomyxoid astrocytoma	3	ORPHANET
Hgene	BRAF	C0004565	Melanoma, B16	2	CTD_human
Hgene	BRAF	C0009075	Melanoma, Cloudman S91	2	CTD_human
Hgene	BRAF	C0018598	Melanoma, Harding-Passey	2	CTD_human
Hgene	BRAF	C0023443	Hairy Cell Leukemia	2	CGI;ORPHANET
Hgene	BRAF	C0025205	Melanoma, Experimental	2	CTD_human
Hgene	BRAF	C0033578	Prostatic Neoplasms	2	CTD_human
Hgene	BRAF	C0152013	Adenocarcinoma of lung (disorder)	2	CGI;CTD_human
Hgene	BRAF	C0376358	Malignant neoplasm of prostate	2	CTD_human
Hgene	BRAF	C0587248	Costello syndrome (disorder)	2	CLINGEN;CTD_human
Hgene	BRAF	C3501843	Nonmedullary Thyroid Carcinoma	2	CTD_human
Hgene	BRAF	C3501844	Familial Nonmedullary Thyroid Cancer	2	CTD_human
Hgene	BRAF	C0002448	Ameloblastoma	1	CTD_human
Hgene	BRAF	C0004114	Astrocytoma	1	CTD_human
Hgene	BRAF	C0010276	Craniopharyngioma	1	CTD_human;ORPHANET
Hgene	BRAF	C0011860	Diabetes Mellitus, Non-Insulin-Dependent	1	CTD_human
Hgene	BRAF	C0017638	Glioma	1	CGI;CTD_human
Hgene	BRAF	C0019621	Histiocytosis, Langerhans-Cell	1	CGI;ORPHANET
Hgene	BRAF	C0022665	Kidney Neoplasm	1	CTD_human
Hgene	BRAF	C0023903	Liver neoplasms	1	CTD_human
Hgene	BRAF	C0024232	Lymphatic Metastasis	1	CTD_human
Hgene	BRAF	C0024694	Mandibular Neoplasms	1	CTD_human
Hgene	BRAF	C0027659	Neoplasms, Experimental	1	CTD_human
Hgene	BRAF	C0027962	Melanocytic nevus	1	GENOMICS_ENGLAND
Hgene	BRAF	C0036920	Sezary Syndrome	1	CTD_human
Hgene	BRAF	C0041409	Turner Syndrome, Male	1	CTD_human
Hgene	BRAF	C0079773	Lymphoma, T-Cell, Cutaneous	1	CTD_human
Hgene	BRAF	C0205768	Subependymal Giant Cell Astrocytoma	1	CTD_human
Hgene	BRAF	C0206686	Adrenocortical carcinoma	1	CTD_human
Hgene	BRAF	C0206754	Neuroendocrine Tumors	1	CTD_human
Hgene	BRAF	C0259783	mixed gliomas	1	CTD_human
Hgene	BRAF	C0278875	Adult Craniopharyngioma	1	CTD_human
Hgene	BRAF	C0280783	Juvenile Pilocytic Astrocytoma	1	CTD_human
Hgene	BRAF	C0280785	Diffuse Astrocytoma	1	CTD_human
Hgene	BRAF	C0334579	Anaplastic astrocytoma	1	CGI;CTD_human
Hgene	BRAF	C0334580	Protoplasmic astrocytoma	1	CTD_human
Hgene	BRAF	C0334581	Gemistocytic astrocytoma	1	CTD_human
Hgene	BRAF	C0334582	Fibrillary Astrocytoma	1	CTD_human
Hgene	BRAF	C0334583	Pilocytic Astrocytoma	1	CGI;CTD_human
Hgene	BRAF	C0338070	Childhood Cerebral Astrocytoma	1	CTD_human
Hgene	BRAF	C0345904	Malignant neoplasm of liver	1	CTD_human
Hgene	BRAF	C0376407	Granulomatous Slack Skin	1	CTD_human
Hgene	BRAF	C0406803	Syringocystadenoma Papilliferum	1	GENOMICS_ENGLAND
Hgene	BRAF	C0431128	Papillary craniopharyngioma	1	CTD_human
Hgene	BRAF	C0431129	Adamantinous Craniopharyngioma	1	CTD_human
Hgene	BRAF	C0547065	Mixed oligoastrocytoma	1	CTD_human
Hgene	BRAF	C0555198	Malignant Glioma	1	CTD_human
Hgene	BRAF	C0596263	Carcinogenesis	1	CTD_human
Hgene	BRAF	C0684249	Carcinoma of lung	1	CGI;UNIPROT
Hgene	BRAF	C0740457	Malignant neoplasm of kidney	1	CTD_human
Hgene	BRAF	C0750935	Cerebral Astrocytoma	1	CTD_human
Hgene	BRAF	C0750936	Intracranial Astrocytoma	1	CTD_human
Hgene	BRAF	C0751061	Craniopharyngioma, Child	1	CTD_human
Hgene	BRAF	C0920269	Microsatellite Instability	1	CTD_human
Hgene	BRAF	C1527404	Female Pseudo-Turner Syndrome	1	CTD_human
Hgene	BRAF	C1704230	Grade I Astrocytoma	1	CTD_human
Hgene	BRAF	C1721098	Replication Error Phenotype	1	CTD_human
Hgene	BRAF	C2239176	Liver carcinoma	1	CTD_human
Hgene	BRAF	C4551484	Leopard Syndrome 1	1	GENOMICS_ENGLAND
Hgene	BRAF	C4551602	Noonan Syndrome 1	1	CTD_human
Hgene	BRAF	C4721532	Lymphoma, Non-Hodgkin, Familial	1	UNIPROT
Hgene	BRAF	C4733333	familial non-medullary thyroid cancer	1	GENOMICS_ENGLAND