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Fusion Protein:ACVR2A-CASP8 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: ACVR2A-CASP8 | FusionPDB ID: 1905 | FusionGDB2.0 ID: 1905 | Hgene | Tgene | Gene symbol | ACVR2A | CASP8 | Gene ID | 92 | 841 |
| Gene name | activin A receptor type 2A | caspase 8 | |
| Synonyms | ACTRII|ACVR2 | ALPS2B|CAP4|Casp-8|FLICE|MACH|MCH5 | |
| Cytomap | 2q22.3-q23.1 | 2q33.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | activin receptor type-2Aactivin A receptor, type IIA | caspase-8FADD-homologous ICE/CED-3-like proteaseFADD-like ICEICE-like apoptotic protease 5MACH-alpha-1/2/3 proteinMACH-beta-1/2/3/4 proteinMORT1-associated ced-3 homologapoptotic cysteine proteaseapoptotic protease Mch-5caspase 8, apoptosis-relat | |
| Modification date | 20200313 | 20200322 | |
| UniProtAcc | P27037 | Q9UKL3 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000241416, ENST00000404590, ENST00000535787, ENST00000495775, | ENST00000264274, ENST00000264275, ENST00000323492, ENST00000392258, ENST00000392259, ENST00000392266, ENST00000358485, ENST00000432109, ENST00000490682, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 8 X 7 X 7=392 | 4 X 4 X 3=48 |
| # samples | 10 | 5 | |
| ** MAII score | log2(10/392*10)=-1.97085365434048 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(5/48*10)=0.0588936890535686 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: ACVR2A [Title/Abstract] AND CASP8 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | ACVR2A(148674995)-CASP8(202131184), # samples:3 | ||
| Anticipated loss of major functional domain due to fusion event. | ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Hgene partner, which is a kinase due to the frame-shifted ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. ACVR2A-CASP8 seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | ACVR2A | GO:0030509 | BMP signaling pathway | 18436533 |
| Hgene | ACVR2A | GO:0032927 | positive regulation of activin receptor signaling pathway | 12665502 |
| Hgene | ACVR2A | GO:0045648 | positive regulation of erythrocyte differentiation | 9032295 |
| Tgene | CASP8 | GO:0006508 | proteolysis | 12888622 |
| Tgene | CASP8 | GO:0036462 | TRAIL-activated apoptotic signaling pathway | 21785459 |
| Tgene | CASP8 | GO:0045862 | positive regulation of proteolysis | 18387192 |
| Tgene | CASP8 | GO:0097191 | extrinsic apoptotic signaling pathway | 21785459 |
| Tgene | CASP8 | GO:0097202 | activation of cysteine-type endopeptidase activity | 18387192 |
| Fusion gene breakpoints across ACVR2A (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across CASP8 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | LUSC | TCGA-21-1082-01A | ACVR2A | chr2 | 148674995 | - | CASP8 | chr2 | 202131184 | + |
| ChimerDB4 | LUSC | TCGA-21-1082-01A | ACVR2A | chr2 | 148674995 | + | CASP8 | chr2 | 202131184 | + |
| ChimerDB4 | LUSC | TCGA-21-1082 | ACVR2A | chr2 | 148674995 | + | CASP8 | chr2 | 202131184 | + |
| ChimerDB4 | LUSC | TCGA-21-1082 | ACVR2A | chr2 | 148674995 | + | CASP8 | chr2 | 202131210 | + |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000535787 | ACVR2A | chr2 | 148674995 | + | ENST00000432109 | CASP8 | chr2 | 202131210 | + | 2328 | 888 | 90 | 2327 | 745 |
| ENST00000241416 | ACVR2A | chr2 | 148674995 | + | ENST00000432109 | CASP8 | chr2 | 202131210 | + | 2892 | 1452 | 636 | 2891 | 751 |
| ENST00000404590 | ACVR2A | chr2 | 148674995 | + | ENST00000432109 | CASP8 | chr2 | 202131210 | + | 2426 | 986 | 170 | 2425 | 752 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000535787 | ENST00000432109 | ACVR2A | chr2 | 148674995 | + | CASP8 | chr2 | 202131210 | + | 0.002276216 | 0.99772376 |
| ENST00000241416 | ENST00000432109 | ACVR2A | chr2 | 148674995 | + | CASP8 | chr2 | 202131210 | + | 0.000623458 | 0.9993766 |
| ENST00000404590 | ENST00000432109 | ACVR2A | chr2 | 148674995 | + | CASP8 | chr2 | 202131210 | + | 0.001343028 | 0.998657 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >1905_1905_1_ACVR2A-CASP8_ACVR2A_chr2_148674995_ENST00000241416_CASP8_chr2_202131210_ENST00000432109_length(amino acids)=751AA_BP=271 MGAAAKLAFAVFLISCSSGAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTD CVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPYYNILLYSLVPLMLIAGIVICAFWVYRHHKMAYPPVLVPTQDPGP PPPSPLLGLKPLQLLEVKARGRFGCVWKAQLLNEYVAVKIFPIQDKQSWQNEYEVYSLPGMKHENILQFIGAEKRGTSVDVDLWLITAFH EKMDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMER ELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDY EEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAG ALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQ ACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNY -------------------------------------------------------------- >1905_1905_2_ACVR2A-CASP8_ACVR2A_chr2_148674995_ENST00000404590_CASP8_chr2_202131210_ENST00000432109_length(amino acids)=752AA_BP=271 MGAAAKLAFAVFLISCSSGAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTD CVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPYYNILLYSLVPLMLIAGIVICAFWVYRHHKMAYPPVLVPTQDPGP PPPSPLLGLKPLQLLEVKARGRFGCVWKAQLLNEYVAVKIFPIQDKQSWQNEYEVYSLPGMKHENILQFIGAEKRGTSVDVDLWLITAFH EKMDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMER ELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDY EEFSKERSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAG ALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQ ACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNY -------------------------------------------------------------- >1905_1905_3_ACVR2A-CASP8_ACVR2A_chr2_148674995_ENST00000535787_CASP8_chr2_202131210_ENST00000432109_length(amino acids)=745AA_BP=265 MPPAAPSPRRSRLAATAASSAASGPRTLGVWVEDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKD SPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPYYNILLYSLVPLMLIAGIVICAFWVYRHHKMAYPPVLVPTQDPGPPPPSPL LGLKPLQLLEVKARGRFGCVWKAQLLNEYVAVKIFPIQDKQSWQNEYEVYSLPGMKHENILQFIGAEKRGTSVDVDLWLITAFHEKMDFS RNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPG RAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKE RSSSLEGSPDEFSNGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTF EELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDN YQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTWYIQSLCQSLRERCPRGDDILTILTEVNYEVSNKD -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr2:148674995/chr2:202131184) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| ACVR2A | CASP8 |
| FUNCTION: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. Mediates induction of adipogenesis by GDF6 (By similarity). {ECO:0000250|UniProtKB:P27038, ECO:0000269|PubMed:1314589}. | FUNCTION: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. {ECO:0000269|PubMed:12477726, ECO:0000269|PubMed:15698540, ECO:0000269|PubMed:17003125, ECO:0000269|PubMed:17245429}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000241416 | + | 6 | 11 | 198_206 | 272.0 | 514.0 | Nucleotide binding | ATP |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000404590 | + | 7 | 12 | 198_206 | 272.0 | 514.0 | Nucleotide binding | ATP |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000241416 | + | 6 | 11 | 20_135 | 272.0 | 514.0 | Topological domain | Extracellular |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000404590 | + | 7 | 12 | 20_135 | 272.0 | 514.0 | Topological domain | Extracellular |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000241416 | + | 6 | 11 | 136_161 | 272.0 | 514.0 | Transmembrane | Helical |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000404590 | + | 7 | 12 | 136_161 | 272.0 | 514.0 | Transmembrane | Helical |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000264274 | 0 | 7 | 100_177 | 0 | 396.0 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000264274 | 0 | 7 | 2_80 | 0 | 396.0 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000264275 | 0 | 10 | 100_177 | 0 | 497.0 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000264275 | 0 | 10 | 2_80 | 0 | 497.0 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000323492 | 0 | 8 | 100_177 | 0 | 465.0 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000323492 | 0 | 8 | 2_80 | 0 | 465.0 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000358485 | 0 | 9 | 100_177 | 0 | 539.0 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000358485 | 0 | 9 | 2_80 | 0 | 539.0 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000392258 | 0 | 8 | 100_177 | 0 | 224.66666666666666 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000392258 | 0 | 8 | 2_80 | 0 | 224.66666666666666 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000392259 | 0 | 9 | 100_177 | 0 | 751.3333333333334 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000392259 | 0 | 9 | 2_80 | 0 | 751.3333333333334 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000392266 | 0 | 8 | 100_177 | 0 | 736.3333333333334 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000392266 | 0 | 8 | 2_80 | 0 | 736.3333333333334 | Domain | DED 1 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000432109 | 1 | 10 | 100_177 | 0.0 | 480.0 | Domain | DED 2 | |
| Tgene | CASP8 | chr2:148674995 | chr2:202131210 | ENST00000432109 | 1 | 10 | 2_80 | 0.0 | 480.0 | Domain | DED 1 |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000241416 | + | 6 | 11 | 192_485 | 272.0 | 514.0 | Domain | Protein kinase |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000404590 | + | 7 | 12 | 192_485 | 272.0 | 514.0 | Domain | Protein kinase |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000241416 | + | 6 | 11 | 162_513 | 272.0 | 514.0 | Topological domain | Cytoplasmic |
| Hgene | ACVR2A | chr2:148674995 | chr2:202131210 | ENST00000404590 | + | 7 | 12 | 162_513 | 272.0 | 514.0 | Topological domain | Cytoplasmic |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| ACVR2A | |
| CASP8 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to ACVR2A-CASP8 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to ACVR2A-CASP8 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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