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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:CTRB2-CEL

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: CTRB2-CEL
	FusionPDB ID: 20434
	FusionGDB2.0 ID: 20434
		Hgene	Tgene
	Gene symbol	CTRB2	CEL
	Gene ID	440387	1056
	Gene name	chymotrypsinogen B2	carboxyl ester lipase
	Synonyms	-	BAL\|BSDL\|BSSL\|CELL\|CEase\|FAP\|FAPP\|LIPA\|MODY8
	Cytomap	16q23.1	9q34.13
	Type of gene	protein-coding	protein-coding
	Description	chymotrypsinogen B2chymotrypsin B2	bile salt-activated lipasebile salt-dependent lipase, oncofetal isoformbucelipasecarboxyl ester hydrolasecarboxyl ester lipase (bile salt-stimulated lipase)cholesterol esterasefetoacinar pancreatic proteinlysophospholipase, pancreaticsterol estera
	Modification date	20200313	20200313
	UniProtAcc	Q6GPI1	Q9NYQ7
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000303037,	ENST00000351304, ENST00000372080,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	7 X 14 X 1=98	5 X 6 X 4=120
	# samples	8	6
	** MAII score	log2(8/98*10)=-0.292781749227846 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(6/120*10)=-1 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: CTRB2 [Title/Abstract] AND CEL [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	CTRB2(75238670)-CEL(135947075), # samples:1 CTRB2(75238670)-CEL(135939855), # samples:1
Anticipated loss of major functional domain due to fusion event.	CTRB2-CEL seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CTRB2-CEL seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. CTRB2-CEL seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CTRB2-CEL seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. CTRB2-CEL seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. CTRB2-CEL seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	CEL	GO:0006707	cholesterol catabolic process	12031288
Tgene	CEL	GO:0030157	pancreatic juice secretion	1854805

Fusion gene breakpoints across CTRB2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across CEL (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PAAD	TCGA-HZ-8003	CTRB2	chr16	75238670	-	CEL	chr9	135947075	+
ChimerDB4	PAAD	TCGA-LB-A9Q5	CTRB2	chr16	75238670	-	CEL	chr9	135939855	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000303037

CTRB2

chr16

75238670

ENST00000351304

CEL

chr9

135939855

2704

674

726

2606

626

ENST00000303037

CTRB2

chr16

75238670

ENST00000372080

CEL

chr9

135939855

2902

674

726

2804

692

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000303037	ENST00000351304	CTRB2	chr16	75238670	-	CEL	chr9	135939855	+	0.005045356	0.99495465
ENST00000303037	ENST00000372080	CTRB2	chr16	75238670	-	CEL	chr9	135939855	+	0.006034132	0.9939659

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>20434_20434_1_CTRB2-CEL_CTRB2_chr16_75238670_ENST00000303037_CEL_chr9_135939855_ENST00000351304_length(amino acids)=626AA_BP=216
MENPQPHPGWQGTLKAKNFKKRCLQATITQDSTYGDEDCLYLNIWVPQGRKQVSRDLPVMIWIYGGAFLMGSGHGANFLNNYLYDGEEIA
TRGNVIVVTFNYRVGPLGFLSTGDANLPGNYGLRDQHMAIAWVKRNIAAFGGDPNNITLFGESAGGASVSLQTLSPYNKGLIRRAISQSG
VALSPWVIQKNPLFWAKKVAEKVGCPVGDAARMAQCLKVTDPRALTLAYKVPLAGLEYPMLHYVGFVPVIDGDFIPADPINLYANAADID
YIAGTNNMDGHIFASIDMPAINKGNKKVTEEDFYKLVSEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIAL
AQHRANAKDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDS
ETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGA

--------------------------------------------------------------

>20434_20434_2_CTRB2-CEL_CTRB2_chr16_75238670_ENST00000303037_CEL_chr9_135939855_ENST00000372080_length(amino acids)=692AA_BP=216
MENPQPHPGWQGTLKAKNFKKRCLQATITQDSTYGDEDCLYLNIWVPQGRKQVSRDLPVMIWIYGGAFLMGSGHGANFLNNYLYDGEEIA
TRGNVIVVTFNYRVGPLGFLSTGDANLPGNYGLRDQHMAIAWVKRNIAAFGGDPNNITLFGESAGGASVSLQTLSPYNKGLIRRAISQSG
VALSPWVIQKNPLFWAKKVAEKVGCPVGDAARMAQCLKVTDPRALTLAYKVPLAGLEYPMLHYVGFVPVIDGDFIPADPINLYANAADID
YIAGTNNMDGHIFASIDMPAINKGNKKVTEEDFYKLVSEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPTEIAL
AQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAYWTNFAKTGDPNMGDSAVPTHWEPY
TTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEATPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGA
PPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPP

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr16:75238670/chr9:135947075)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
CTRB2 Q6GPI1	CEL Q9NYQ7
	FUNCTION: Receptor that may have an important role in cell/cell signaling during nervous system formation.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

21_121

688.0

Region

Note=Heparin-binding

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

559_745

688.0

Region

Note=17 X 11 AA tandem repeats%2C glycodomain%2C O-linked (mucin type)

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

559_569

688.0

Repeat

Note=1

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

570_580

688.0

Repeat

Note=2

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

581_591

688.0

Repeat

Note=3

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

592_602

688.0

Repeat

Note=4

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

603_613

688.0

Repeat

Note=5

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

614_624

688.0

Repeat

Note=6

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

625_635

688.0

Repeat

Note=7

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

636_646

688.0

Repeat

Note=8

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

647_657

688.0

Repeat

Note=9

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

658_668

688.0

Repeat

Note=10

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

669_679

688.0

Repeat

Note=11

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

680_690

688.0

Repeat

Note=12

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

691_701

688.0

Repeat

Note=13

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

702_712

688.0

Repeat

Note=14

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

713_723

688.0

Repeat

Note=15

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

724_734

688.0

Repeat

Note=16

Tgene

CEL

chr16:75238670

chr9:135939855

ENST00000351304

735_745

688.0

Repeat

Note=17

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

CTRB2

chr16:75238670

chr9:135939855

ENST00000303037

34_261

210.0

264.0

Domain

Peptidase S1

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
CTRB2
CEL

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to CTRB2-CEL

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to CTRB2-CEL

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source