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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:DEPDC5-BID

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: DEPDC5-BID
	FusionPDB ID: 22308
	FusionGDB2.0 ID: 22308
		Hgene	Tgene
	Gene symbol	DEPDC5	BID
	Gene ID	9681	637
	Gene name	DEP domain containing 5, GATOR1 subcomplex subunit	BH3 interacting domain death agonist
	Synonyms	DEP.5\|FFEVF\|FFEVF1	FP497
	Cytomap	22q12.2-q12.3	22q11.21
	Type of gene	protein-coding	protein-coding
	Description	GATOR complex protein DEPDC5DEP domain containing 5DEP domain-containing protein 5	BH3-interacting domain death agonistBH3 interacting domain death agonist Si6 isoformBID isoform ES(1b)BID isoform L(2)BID isoform Si6Human BID coding sequenceapoptic death agonistdesmocollin type 4p22 BID
	Modification date	20200315	20200313
	UniProtAcc	O75140	P55957
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000266091, ENST00000382105, ENST00000382111, ENST00000382112, ENST00000400242, ENST00000400246, ENST00000400248, ENST00000400249, ENST00000535622, ENST00000536766, ENST00000494060, ENST00000539165,	ENST00000342111, ENST00000399774, ENST00000551952, ENST00000399765, ENST00000399767, ENST00000473439, ENST00000317361,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	15 X 15 X 5=1125	8 X 6 X 7=336
	# samples	14	9
	** MAII score	log2(14/1125*10)=-3.00642626915943 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(9/336*10)=-1.90046432644909 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: DEPDC5 [Title/Abstract] AND BID [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	DEPDC5(32154619)-BID(18232940), # samples:4
Anticipated loss of major functional domain due to fusion event.	DEPDC5-BID seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DEPDC5-BID seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. DEPDC5-BID seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. DEPDC5-BID seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	BID	GO:0001836	release of cytochrome c from mitochondria	17052454
Tgene	BID	GO:0031334	positive regulation of protein complex assembly	19074440\|21041309
Tgene	BID	GO:0090150	establishment of protein localization to membrane	21041309

Fusion gene breakpoints across DEPDC5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across BID (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	UCEC	TCGA-AJ-A3EM-01A	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-
ChimerDB4	UCEC	TCGA-AJ-A3EM-01A	DEPDC5	chr22	32154619	-	BID	chr22	18232940	-
ChimerDB4	UCEC	TCGA-AJ-A3EM-01A	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-
ChimerDB4	UCEC	TCGA-AJ-A3EM	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000535622	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2310	224	78	869	263
ENST00000400246	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2374	288	142	933	263
ENST00000266091	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2374	288	142	933	263
ENST00000382105	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2374	288	142	933	263
ENST00000400249	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2374	288	142	933	263
ENST00000382112	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2302	216	70	861	263
ENST00000382111	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2292	206	60	851	263
ENST00000400248	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2291	205	59	850	263
ENST00000536766	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2403	317	171	962	263
ENST00000400242	DEPDC5	chr22	32154619	+	ENST00000317361	BID	chr22	18232940	-	2388	302	156	947	263
ENST00000535622	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2310	224	78	869	263
ENST00000400246	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2374	288	142	933	263
ENST00000266091	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2374	288	142	933	263
ENST00000382105	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2374	288	142	933	263
ENST00000400249	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2374	288	142	933	263
ENST00000382112	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2302	216	70	861	263
ENST00000382111	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2292	206	60	851	263
ENST00000400248	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2291	205	59	850	263
ENST00000536766	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2403	317	171	962	263
ENST00000400242	DEPDC5	chr22	32154618	+	ENST00000317361	BID	chr22	18232939	-	2388	302	156	947	263

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000535622	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003565788	0.9964342
ENST00000400246	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003681773	0.9963182
ENST00000266091	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003681773	0.9963182
ENST00000382105	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003681773	0.9963182
ENST00000400249	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003681773	0.9963182
ENST00000382112	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003862571	0.99613744
ENST00000382111	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003403471	0.9965965
ENST00000400248	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.003526398	0.9964736
ENST00000536766	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.00377807	0.9962219
ENST00000400242	ENST00000317361	DEPDC5	chr22	32154619	+	BID	chr22	18232940	-	0.00394577	0.9960542
ENST00000535622	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003565788	0.9964342
ENST00000400246	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003681773	0.9963182
ENST00000266091	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003681773	0.9963182
ENST00000382105	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003681773	0.9963182
ENST00000400249	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003681773	0.9963182
ENST00000382112	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003862571	0.99613744
ENST00000382111	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003403471	0.9965965
ENST00000400248	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.003526398	0.9964736
ENST00000536766	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.00377807	0.9962219
ENST00000400242	ENST00000317361	DEPDC5	chr22	32154618	+	BID	chr22	18232939	-	0.00394577	0.9960542

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>22308_22308_1_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000266091_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_2_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000382105_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_3_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000382111_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_4_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000382112_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_5_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000400242_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_6_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000400246_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_7_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000400248_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_8_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000400249_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_9_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000535622_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_10_DEPDC5-BID_DEPDC5_chr22_32154618_ENST00000536766_BID_chr22_18232939_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_11_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000266091_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_12_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000382105_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_13_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000382111_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_14_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000382112_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_15_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000400242_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_16_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000400246_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_17_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000400248_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_18_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000400249_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_19_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000535622_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

>22308_22308_20_DEPDC5-BID_DEPDC5_chr22_32154619_ENST00000536766_BID_chr22_18232940_ENST00000317361_length(amino acids)=263AA_BP=48
MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF
GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr22:32154619/chr22:18232940)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
DEPDC5 O75140	BID P55957
FUNCTION: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway. {ECO:0000269\|PubMed:23723238, ECO:0000269\|PubMed:25457612, ECO:0000269\|PubMed:29769719}.	FUNCTION: The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2. {ECO:0000250\|UniProtKB:P70444, ECO:0000269\|PubMed:14583606}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

BID

chr22:32154618

chr22:18232939

ENST00000317361

86_100

26.666666666666668

242.0

Motif

BH3

Tgene

BID

chr22:32154618

chr22:18232939

ENST00000342111

86_100

182.33333333333334

Motif

BH3

Tgene

BID

chr22:32154618

chr22:18232939

ENST00000399765

86_100

100.0

Motif

BH3

Tgene

BID

chr22:32154618

chr22:18232939

ENST00000399767

86_100

100.0

Motif

BH3

Tgene

BID

chr22:32154618

chr22:18232939

ENST00000399774

86_100

196.0

Motif

BH3

Tgene

BID

chr22:32154618

chr22:18232939

ENST00000551952

86_100

196.0

Motif

BH3

Tgene

BID

chr22:32154619

chr22:18232940

ENST00000317361

86_100

26.666666666666668

242.0

Motif

BH3

Tgene

BID

chr22:32154619

chr22:18232940

ENST00000342111

86_100

182.33333333333334

Motif

BH3

Tgene

BID

chr22:32154619

chr22:18232940

ENST00000399765

86_100

100.0

Motif

BH3

Tgene

BID

chr22:32154619

chr22:18232940

ENST00000399767

86_100

100.0

Motif

BH3

Tgene

BID

chr22:32154619

chr22:18232940

ENST00000399774

86_100

196.0

Motif

BH3

Tgene

BID

chr22:32154619

chr22:18232940

ENST00000551952

86_100

196.0

Motif

BH3

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000266091

1187_1262

48.666666666666664

1582.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000382111

1187_1262

48.666666666666664

1553.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000382112

1187_1262

48.666666666666664

1595.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000400242

1187_1262

48.666666666666664

560.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000400246

1187_1262

48.666666666666664

1553.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000400248

1187_1262

48.666666666666664

1573.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000400249

1187_1262

48.666666666666664

1573.0

Domain

DEP

Hgene

DEPDC5

chr22:32154618

chr22:18232939

ENST00000535622

1187_1262

48.666666666666664

1504.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000266091

1187_1262

48.666666666666664

1582.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000382111

1187_1262

48.666666666666664

1553.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000382112

1187_1262

48.666666666666664

1595.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000400242

1187_1262

48.666666666666664

560.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000400246

1187_1262

48.666666666666664

1553.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000400248

1187_1262

48.666666666666664

1573.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000400249

1187_1262

48.666666666666664

1573.0

Domain

DEP

Hgene

DEPDC5

chr22:32154619

chr22:18232940

ENST00000535622

1187_1262

48.666666666666664

1504.0

Domain

DEP

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>450_DEPDC5_32154619_BID_18232940_ranked_0.pdb	DEPDC5	32154618	32154619	ENST00000317361	BID	chr22	18232940	-	MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYRSPLGHCEPGVSRSCRAAQAMDCEVNNGSSLRDECITNLLVF GFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQ	263

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

DEPDC5_pLDDT.png

BID_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
DEPDC5
BID

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to DEPDC5-BID

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to DEPDC5-BID

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source