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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:EPHA2-LSM14B

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: EPHA2-LSM14B
	FusionPDB ID: 26954
	FusionGDB2.0 ID: 26954
		Hgene	Tgene
	Gene symbol	EPHA2	LSM14B
	Gene ID	1969	149986
	Gene name	EPH receptor A2	LSM family member 14B
	Synonyms	ARCC2\|CTPA\|CTPP1\|CTRCT6\|ECK	C20orf40\|FAM61B\|FT005\|LSM13\|RAP55B\|bA11M20.3
	Cytomap	1p36.13	20q13.33
	Type of gene	protein-coding	protein-coding
	Description	ephrin type-A receptor 2epithelial cell receptor protein tyrosine kinasesoluble EPHA2 variant 1tyrosine-protein kinase receptor ECK	protein LSM14 homolog BLSM14 homolog BLSM14B, SCD6 homolog BRNA-associated protein 55Bfamily with sequence similarity 61, member BhRAP55B
	Modification date	20200313	20200327
	UniProtAcc	P29317	Q9BX40
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000358432, ENST00000461614,	ENST00000253001, ENST00000279068, ENST00000370915,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 5 X 4=120	3 X 4 X 2=24
	# samples	8	4
	** MAII score	log2(8/120*10)=-0.584962500721156 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/24*10)=0.736965594166206 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: EPHA2 [Title/Abstract] AND LSM14B [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	EPHA2(16474873)-LSM14B(60699673), # samples:3
Anticipated loss of major functional domain due to fusion event.	EPHA2-LSM14B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EPHA2-LSM14B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. EPHA2-LSM14B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. EPHA2-LSM14B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	EPHA2	GO:0008630	intrinsic apoptotic signaling pathway in response to DNA damage	18339848
Hgene	EPHA2	GO:0033628	regulation of cell adhesion mediated by integrin	10655584
Hgene	EPHA2	GO:0043491	protein kinase B signaling	19573808
Hgene	EPHA2	GO:0048013	ephrin receptor signaling pathway	10655584\|20861311
Hgene	EPHA2	GO:0051898	negative regulation of protein kinase B signaling	19573808

Fusion gene breakpoints across EPHA2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across LSM14B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	UCS	TCGA-N9-A4PZ-01A	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+
ChimerDB4	UCS	TCGA-N9-A4PZ	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+
ChimerDB4	UCS	TCGA-N9-A4PZ	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000358432	EPHA2	chr1	16474873	-	ENST00000370915	LSM14B	chr20	60699673	+	1724	978	155	1516	453
ENST00000358432	EPHA2	chr1	16474873	-	ENST00000253001	LSM14B	chr20	60699673	+	3239	978	155	2008	617
ENST00000358432	EPHA2	chr1	16474873	-	ENST00000279068	LSM14B	chr20	60699673	+	3239	978	155	2008	617
ENST00000461614	EPHA2	chr1	16474873	-	ENST00000370915	LSM14B	chr20	60699673	+	1621	875	13	1413	466
ENST00000461614	EPHA2	chr1	16474873	-	ENST00000253001	LSM14B	chr20	60699673	+	3136	875	13	1905	630
ENST00000461614	EPHA2	chr1	16474873	-	ENST00000279068	LSM14B	chr20	60699673	+	3136	875	13	1905	630
ENST00000358432	EPHA2	chr1	16474872	-	ENST00000370915	LSM14B	chr20	60699672	+	1724	978	155	1516	453
ENST00000358432	EPHA2	chr1	16474872	-	ENST00000253001	LSM14B	chr20	60699672	+	3239	978	155	2008	617
ENST00000358432	EPHA2	chr1	16474872	-	ENST00000279068	LSM14B	chr20	60699672	+	3239	978	155	2008	617
ENST00000461614	EPHA2	chr1	16474872	-	ENST00000370915	LSM14B	chr20	60699672	+	1621	875	13	1413	466
ENST00000461614	EPHA2	chr1	16474872	-	ENST00000253001	LSM14B	chr20	60699672	+	3136	875	13	1905	630
ENST00000461614	EPHA2	chr1	16474872	-	ENST00000279068	LSM14B	chr20	60699672	+	3136	875	13	1905	630

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000358432	ENST00000370915	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+	0.009616244	0.99038374
ENST00000358432	ENST00000253001	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+	0.001953909	0.99804604
ENST00000358432	ENST00000279068	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+	0.001545863	0.9984541
ENST00000461614	ENST00000370915	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+	0.012815283	0.9871847
ENST00000461614	ENST00000253001	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+	0.002736484	0.99726355
ENST00000461614	ENST00000279068	EPHA2	chr1	16474873	-	LSM14B	chr20	60699673	+	0.002154937	0.99784505
ENST00000358432	ENST00000370915	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+	0.009616244	0.99038374
ENST00000358432	ENST00000253001	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+	0.001953909	0.99804604
ENST00000358432	ENST00000279068	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+	0.001545863	0.9984541
ENST00000461614	ENST00000370915	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+	0.012815283	0.9871847
ENST00000461614	ENST00000253001	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+	0.002736484	0.99726355
ENST00000461614	ENST00000279068	EPHA2	chr1	16474872	-	LSM14B	chr20	60699672	+	0.002154937	0.99784505

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>26954_26954_1_EPHA2-LSM14B_EPHA2_chr1_16474872_ENST00000358432_LSM14B_chr20_60699672_ENST00000253001_length(amino acids)=617AA_BP=274
MELQAARACFALLWGCALAAAAAAQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGE
AERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYL
AFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVE
DACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYSPFRGMAPYGPLAA
SSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAADRMTVAVADHLPSP
CSRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQSAEAPAEEDLLGP

--------------------------------------------------------------

>26954_26954_2_EPHA2-LSM14B_EPHA2_chr1_16474872_ENST00000358432_LSM14B_chr20_60699672_ENST00000279068_length(amino acids)=617AA_BP=274
MELQAARACFALLWGCALAAAAAAQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGE
AERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYL
AFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVE
DACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYSPFRGMAPYGPLAA
SSLLSQQYAASLGLGAGFPSIPVGKSPMVEQAVQTGSADNLNAKKLLPGKGTTGTQLNGRQAQPSSKTASDVVQPAAVQAQGQVNDENRR
PQRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQSAEAPAEEDLLGP

--------------------------------------------------------------

>26954_26954_3_EPHA2-LSM14B_EPHA2_chr1_16474872_ENST00000358432_LSM14B_chr20_60699672_ENST00000370915_length(amino acids)=453AA_BP=274
MELQAARACFALLWGCALAAAAAAQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGE
AERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYL
AFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVE
DACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYSPFRGMAPYGPLAA
SSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAADRMTVAVADHLPSP

--------------------------------------------------------------

>26954_26954_4_EPHA2-LSM14B_EPHA2_chr1_16474872_ENST00000461614_LSM14B_chr20_60699672_ENST00000253001_length(amino acids)=630AA_BP=287
MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ
DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE
RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI
GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS
PFRGMAPYGPLAASSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAAD
RMTVAVADHLPSPCSRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQ
SAEAPAEEDLLGPNCYYDKSKSFFDNISSELKTSSRRTTWAEERKLNTETFGVSGRFLRGRSSRGGFRGGRGNGTTRRNPTSHRAGTGRV

--------------------------------------------------------------

>26954_26954_5_EPHA2-LSM14B_EPHA2_chr1_16474872_ENST00000461614_LSM14B_chr20_60699672_ENST00000279068_length(amino acids)=630AA_BP=287
MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ
DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE
RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI
GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS
PFRGMAPYGPLAASSLLSQQYAASLGLGAGFPSIPVGKSPMVEQAVQTGSADNLNAKKLLPGKGTTGTQLNGRQAQPSSKTASDVVQPAA
VQAQGQVNDENRRPQRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQ
SAEAPAEEDLLGPNCYYDKSKSFFDNISSELKTSSRRTTWAEERKLNTETFGVSGRFLRGRSSRGGFRGGRGNGTTRRNPTSHRAGTGRV

--------------------------------------------------------------

>26954_26954_6_EPHA2-LSM14B_EPHA2_chr1_16474872_ENST00000461614_LSM14B_chr20_60699672_ENST00000370915_length(amino acids)=466AA_BP=287
MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ
DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE
RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI
GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS
PFRGMAPYGPLAASSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAAD

--------------------------------------------------------------

>26954_26954_7_EPHA2-LSM14B_EPHA2_chr1_16474873_ENST00000358432_LSM14B_chr20_60699673_ENST00000253001_length(amino acids)=617AA_BP=274
MELQAARACFALLWGCALAAAAAAQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGE
AERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYL
AFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVE
DACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYSPFRGMAPYGPLAA
SSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAADRMTVAVADHLPSP
CSRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQSAEAPAEEDLLGP

--------------------------------------------------------------

>26954_26954_8_EPHA2-LSM14B_EPHA2_chr1_16474873_ENST00000358432_LSM14B_chr20_60699673_ENST00000279068_length(amino acids)=617AA_BP=274
MELQAARACFALLWGCALAAAAAAQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGE
AERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYL
AFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVE
DACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYSPFRGMAPYGPLAA
SSLLSQQYAASLGLGAGFPSIPVGKSPMVEQAVQTGSADNLNAKKLLPGKGTTGTQLNGRQAQPSSKTASDVVQPAAVQAQGQVNDENRR
PQRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQSAEAPAEEDLLGP

--------------------------------------------------------------

>26954_26954_9_EPHA2-LSM14B_EPHA2_chr1_16474873_ENST00000358432_LSM14B_chr20_60699673_ENST00000370915_length(amino acids)=453AA_BP=274
MELQAARACFALLWGCALAAAAAAQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGE
AERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYL
AFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVE
DACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYSPFRGMAPYGPLAA
SSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAADRMTVAVADHLPSP

--------------------------------------------------------------

>26954_26954_10_EPHA2-LSM14B_EPHA2_chr1_16474873_ENST00000461614_LSM14B_chr20_60699673_ENST00000253001_length(amino acids)=630AA_BP=287
MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ
DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE
RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI
GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS
PFRGMAPYGPLAASSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAAD
RMTVAVADHLPSPCSRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQ
SAEAPAEEDLLGPNCYYDKSKSFFDNISSELKTSSRRTTWAEERKLNTETFGVSGRFLRGRSSRGGFRGGRGNGTTRRNPTSHRAGTGRV

--------------------------------------------------------------

>26954_26954_11_EPHA2-LSM14B_EPHA2_chr1_16474873_ENST00000461614_LSM14B_chr20_60699673_ENST00000279068_length(amino acids)=630AA_BP=287
MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ
DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE
RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI
GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS
PFRGMAPYGPLAASSLLSQQYAASLGLGAGFPSIPVGKSPMVEQAVQTGSADNLNAKKLLPGKGTTGTQLNGRQAQPSSKTASDVVQPAA
VQAQGQVNDENRRPQRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQ
SAEAPAEEDLLGPNCYYDKSKSFFDNISSELKTSSRRTTWAEERKLNTETFGVSGRFLRGRSSRGGFRGGRGNGTTRRNPTSHRAGTGRV

--------------------------------------------------------------

>26954_26954_12_EPHA2-LSM14B_EPHA2_chr1_16474873_ENST00000461614_LSM14B_chr20_60699673_ENST00000370915_length(amino acids)=466AA_BP=287
MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ
DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE
RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI
GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS
PFRGMAPYGPLAASSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAAD

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:16474873/chr20:60699673)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
EPHA2 P29317	LSM14B Q9BX40
FUNCTION: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. {ECO:0000269\|PubMed:10655584, ECO:0000269\|PubMed:16236711, ECO:0000269\|PubMed:18339848, ECO:0000269\|PubMed:19573808, ECO:0000269\|PubMed:20679435, ECO:0000269\|PubMed:20861311, ECO:0000269\|PubMed:23358419, ECO:0000269\|PubMed:26158630, ECO:0000269\|PubMed:27385333}.; FUNCTION: (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. {ECO:0000269\|PubMed:21516087}.	FUNCTION: Required for oocyte meiotic maturation. May be involved in the storage of translationally inactive mRNAs and protect them from degradation (By similarity). Plays a role in control of mRNA translation (By similarity). {ECO:0000250\|UniProtKB:Q68FI1, ECO:0000250\|UniProtKB:Q8CGC4}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

28_206

274.3333333333333

977.0

Domain

Eph LBD

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

28_206

274.3333333333333

977.0

Domain

Eph LBD

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000253001

241_277

42.333333333333336

680.3333333333334

Domain

DFDF

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000279068

241_277

42.333333333333336

695.6666666666666

Domain

DFDF

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000370915

241_277

42.333333333333336

222.0

Domain

DFDF

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000253001

241_277

42.333333333333336

680.3333333333334

Domain

DFDF

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000279068

241_277

42.333333333333336

695.6666666666666

Domain

DFDF

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000370915

241_277

42.333333333333336

222.0

Domain

DFDF

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000253001

310_326

42.333333333333336

680.3333333333334

Motif

Note=FFD box

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000253001

330_350

42.333333333333336

680.3333333333334

Motif

Note=TFG box

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000279068

310_326

42.333333333333336

695.6666666666666

Motif

Note=FFD box

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000279068

330_350

42.333333333333336

695.6666666666666

Motif

Note=TFG box

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000370915

310_326

42.333333333333336

222.0

Motif

Note=FFD box

Tgene

LSM14B

chr1:16474872

chr20:60699672

ENST00000370915

330_350

42.333333333333336

222.0

Motif

Note=TFG box

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000253001

310_326

42.333333333333336

680.3333333333334

Motif

Note=FFD box

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000253001

330_350

42.333333333333336

680.3333333333334

Motif

Note=TFG box

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000279068

310_326

42.333333333333336

695.6666666666666

Motif

Note=FFD box

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000279068

330_350

42.333333333333336

695.6666666666666

Motif

Note=TFG box

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000370915

310_326

42.333333333333336

222.0

Motif

Note=FFD box

Tgene

LSM14B

chr1:16474873

chr20:60699673

ENST00000370915

330_350

42.333333333333336

222.0

Motif

Note=TFG box

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

188_325

274.3333333333333

977.0

Compositional bias

Note=Cys-rich

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

188_325

274.3333333333333

977.0

Compositional bias

Note=Cys-rich

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

328_432

274.3333333333333

977.0

Domain

Fibronectin type-III 1

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

438_529

274.3333333333333

977.0

Domain

Fibronectin type-III 2

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

613_875

274.3333333333333

977.0

Domain

Protein kinase

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

904_968

274.3333333333333

977.0

Domain

SAM

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

328_432

274.3333333333333

977.0

Domain

Fibronectin type-III 1

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

438_529

274.3333333333333

977.0

Domain

Fibronectin type-III 2

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

613_875

274.3333333333333

977.0

Domain

Protein kinase

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

904_968

274.3333333333333

977.0

Domain

SAM

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

974_976

274.3333333333333

977.0

Motif

PDZ-binding

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

974_976

274.3333333333333

977.0

Motif

PDZ-binding

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

619_627

274.3333333333333

977.0

Nucleotide binding

ATP

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

619_627

274.3333333333333

977.0

Nucleotide binding

ATP

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

24_537

274.3333333333333

977.0

Topological domain

Extracellular

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

559_976

274.3333333333333

977.0

Topological domain

Cytoplasmic

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

24_537

274.3333333333333

977.0

Topological domain

Extracellular

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

559_976

274.3333333333333

977.0

Topological domain

Cytoplasmic

Hgene

EPHA2

chr1:16474872

chr20:60699672

ENST00000358432

538_558

274.3333333333333

977.0

Transmembrane

Helical

Hgene

EPHA2

chr1:16474873

chr20:60699673

ENST00000358432

538_558

274.3333333333333

977.0

Transmembrane

Helical

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1326_EPHA2_16474873_LSM14B_60699673_1326_EPHA2_16474873_LSM14B_60699673_ranked_0.pdb	EPHA2	16474872	16474873	ENST00000279068	LSM14B	chr20	60699673	+	MRAGRRAGADTEAGVQACGCAGAGLGGIGPRARSAAWSSRQPAPASPCCGAVRWPRPRRRRARKWDLMQNIMNDMPIYMYSVCNVMSGDQ DNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEE RSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPI GQCLCQAGYEKVEDACQVRSFGTEDRPTDRPAPPREEIYEYIIFRGSDIKDITVCEPPKAQHTLPQDPAIVQSSLGSASASPFQPHVPYS PFRGMAPYGPLAASSLLSQQYAASLGLEKLVSPPASAAASSPSSSPSPQPVSELDLSSEPQQLTAKGNSSLGELHAVLQTILRARGKAAD RMTVAVADHLPSPCSRRRSGNRRTRNRSRGQNRPTNVKENTIKFEGDFDFESANAQFNREELDKEFKKKLNFKDDKAEKGEEKDLAVVTQ SAEAPAEEDLLGPNCYYDKSKSFFDNISSELKTSSRRTTWAEERKLNTETFGVSGRFLRGRSSRGGFRGGRGNGTTRRNPTSHRAGTGRV	630

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

EPHA2_pLDDT.png

LSM14B_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
EPHA2
LSM14B

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to EPHA2-LSM14B

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to EPHA2-LSM14B

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source