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Fusion Protein:FZD4-LAMTOR1 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: FZD4-LAMTOR1 | FusionPDB ID: 31951 | FusionGDB2.0 ID: 31951 | Hgene | Tgene | Gene symbol | FZD4 | LAMTOR1 | Gene ID | 8322 | 55004 |
| Gene name | frizzled class receptor 4 | late endosomal/lysosomal adaptor, MAPK and MTOR activator 1 | |
| Synonyms | CD344|EVR1|FEVR|FZD4S|Fz-4|Fz4|FzE4|GPCR|hFz4 | C11orf59|PDRO|Ragulator1|p18|p27RF-Rho | |
| Cytomap | 11q14.2 | 11q13.4 | |
| Type of gene | protein-coding | protein-coding | |
| Description | frizzled-4WNT receptor frizzled-4frizzled 4, seven transmembrane spanning receptorfrizzled family receptor 4frizzled homolog 4 | ragulator complex protein LAMTOR1RhoA activator C11orf59late endosomal/lysosomal adaptor and MAPK and MTOR activator 1lipid raft adaptor protein p18p27Kip1-releasing factor from RhoAp27kip1 releasing factor from RhoAprotein associated with DRMs and | |
| Modification date | 20200313 | 20200327 | |
| UniProtAcc | Q9ULV1 | Q6IAA8 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000531380, | ENST00000539797, ENST00000278671, ENST00000535107, ENST00000538404, ENST00000545249, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 5 X 4 X 3=60 | 2 X 2 X 3=12 |
| # samples | 5 | 3 | |
| ** MAII score | log2(5/60*10)=-0.263034405833794 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(3/12*10)=1.32192809488736 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: FZD4 [Title/Abstract] AND LAMTOR1 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | FZD4(86665843)-LAMTOR1(71810302), # samples:3 | ||
| Anticipated loss of major functional domain due to fusion event. | FZD4-LAMTOR1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FZD4-LAMTOR1 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. FZD4-LAMTOR1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. FZD4-LAMTOR1 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | FZD4 | GO:0007223 | Wnt signaling pathway, calcium modulating pathway | 12172548 |
| Hgene | FZD4 | GO:0045893 | positive regulation of transcription, DNA-templated | 15035989|17955262 |
| Hgene | FZD4 | GO:0051091 | positive regulation of DNA-binding transcription factor activity | 14688793 |
| Hgene | FZD4 | GO:0060070 | canonical Wnt signaling pathway | 14688793|20802536|28733458 |
| Fusion gene breakpoints across FZD4 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across LAMTOR1 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | BRCA | TCGA-BH-A0W3-01A | FZD4 | chr11 | 86665843 | - | LAMTOR1 | chr11 | 71810302 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000531380 | FZD4 | chr11 | 86665843 | - | ENST00000545249 | LAMTOR1 | chr11 | 71810302 | - | 1492 | 591 | 258 | 977 | 239 |
| ENST00000531380 | FZD4 | chr11 | 86665843 | - | ENST00000535107 | LAMTOR1 | chr11 | 71810302 | - | 1155 | 591 | 11 | 625 | 204 |
| ENST00000531380 | FZD4 | chr11 | 86665843 | - | ENST00000278671 | LAMTOR1 | chr11 | 71810302 | - | 1565 | 591 | 258 | 1034 | 258 |
| ENST00000531380 | FZD4 | chr11 | 86665843 | - | ENST00000538404 | LAMTOR1 | chr11 | 71810302 | - | 1883 | 591 | 258 | 1031 | 257 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000531380 | ENST00000545249 | FZD4 | chr11 | 86665843 | - | LAMTOR1 | chr11 | 71810302 | - | 0.026955197 | 0.9730449 |
| ENST00000531380 | ENST00000535107 | FZD4 | chr11 | 86665843 | - | LAMTOR1 | chr11 | 71810302 | - | 0.013860488 | 0.98613954 |
| ENST00000531380 | ENST00000278671 | FZD4 | chr11 | 86665843 | - | LAMTOR1 | chr11 | 71810302 | - | 0.023344057 | 0.9766559 |
| ENST00000531380 | ENST00000538404 | FZD4 | chr11 | 86665843 | - | LAMTOR1 | chr11 | 71810302 | - | 0.14693077 | 0.85306925 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >31951_31951_1_FZD4-LAMTOR1_FZD4_chr11_86665843_ENST00000531380_LAMTOR1_chr11_71810302_ENST00000278671_length(amino acids)=258AA_BP=111 MPEQPQPLPLLPPMLAMAWRGAGPSVPGAPGGVGLSLGLLLQLLLLLGPARGFGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTD AELQLTTFTPLIQYGCSSQLQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMD -------------------------------------------------------------- >31951_31951_2_FZD4-LAMTOR1_FZD4_chr11_86665843_ENST00000531380_LAMTOR1_chr11_71810302_ENST00000535107_length(amino acids)=204AA_BP= MAAGWPRGDAERTRPAPACGLAACSHDPRSLSLGLGPGRLKSHTVARSCWRAGRCPLVAASHSRPGSWEQRGQPAPPCKLGVSARAAPAA AAATPDAGHGLAGRRAERPGGARGRRSQSGVAPAVAAAPGAGAGLRGRGRAALRPHPHLHVPEPRLQRDQDAQPGWARAADGRRAAADNF -------------------------------------------------------------- >31951_31951_3_FZD4-LAMTOR1_FZD4_chr11_86665843_ENST00000531380_LAMTOR1_chr11_71810302_ENST00000538404_length(amino acids)=257AA_BP=111 MPEQPQPLPLLPPMLAMAWRGAGPSVPGAPGGVGLSLGLLLQLLLLLGPARGFGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTD AELQLTTFTPLIQYGCSSQLQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMD -------------------------------------------------------------- >31951_31951_4_FZD4-LAMTOR1_FZD4_chr11_86665843_ENST00000531380_LAMTOR1_chr11_71810302_ENST00000545249_length(amino acids)=239AA_BP=111 MPEQPQPLPLLPPMLAMAWRGAGPSVPGAPGGVGLSLGLLLQLLLLLGPARGFGDEEERRCDPIRISMCQNLGYNVTKMPNLVGHELQTD AELQLTTFTPLIQYGCSSQLQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMD -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:86665843/chr11:71810302) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| FZD4 | LAMTOR1 |
| FUNCTION: Receptor for Wnt proteins (PubMed:30135577). Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes (PubMed:30135577). Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP) (By similarity). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. {ECO:0000250|UniProtKB:Q61088, ECO:0000269|PubMed:30135577}. | FUNCTION: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation. {ECO:0000269|PubMed:19654316, ECO:0000269|PubMed:20381137, ECO:0000269|PubMed:20544018, ECO:0000269|PubMed:22980980}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 40_161 | 95.0 | 538.0 | Domain | FZ |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 535_537 | 95.0 | 538.0 | Motif | Note=PDZ-binding |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 244_249 | 95.0 | 538.0 | Topological domain | Cytoplasmic |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 276_299 | 95.0 | 538.0 | Topological domain | Extracellular |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 334_336 | 95.0 | 538.0 | Topological domain | Cytoplasmic |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 366_383 | 95.0 | 538.0 | Topological domain | Extracellular |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 37_212 | 95.0 | 538.0 | Topological domain | Extracellular |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 419_431 | 95.0 | 538.0 | Topological domain | Cytoplasmic |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 461_473 | 95.0 | 538.0 | Topological domain | Extracellular |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 496_537 | 95.0 | 538.0 | Topological domain | Cytoplasmic |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 250_275 | 95.0 | 538.0 | Transmembrane | Helical%3B Name%3D2 |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 300_333 | 95.0 | 538.0 | Transmembrane | Helical%3B Name%3D3 |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 337_365 | 95.0 | 538.0 | Transmembrane | Helical%3B Name%3D4 |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 384_418 | 95.0 | 538.0 | Transmembrane | Helical%3B Name%3D5 |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 432_460 | 95.0 | 538.0 | Transmembrane | Helical%3B Name%3D6 |
| Hgene | FZD4 | chr11:86665843 | chr11:71810302 | ENST00000531380 | - | 1 | 2 | 474_495 | 95.0 | 538.0 | Transmembrane | Helical%3B Name%3D7 |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| FZD4 | |
| LAMTOR1 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to FZD4-LAMTOR1 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to FZD4-LAMTOR1 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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