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Fusion Protein:GAS7-CHMP4A |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: GAS7-CHMP4A | FusionPDB ID: 32554 | FusionGDB2.0 ID: 32554 | Hgene | Tgene | Gene symbol | GAS7 | CHMP4A | Gene ID | 8522 | 128866 |
| Gene name | growth arrest specific 7 | charged multivesicular body protein 4B | |
| Synonyms | - | C20orf178|CHMP4A|CTPP3|CTRCT31|SNF7|SNF7-2|Shax1|VPS32B|Vps32-2|dJ553F4.4 | |
| Cytomap | 17p13.1 | 20q11.22 | |
| Type of gene | protein-coding | protein-coding | |
| Description | growth arrest-specific protein 7 | charged multivesicular body protein 4bSNF7 homolog associated with Alix 1Snf7 homologue associated with Alix 1chromatin modifying protein 4Bchromatin-modifying protein 4bhSnf7-2hVps32-2vacuolar protein-sorting-associated protein 32-2 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | O60861 | Q9BY43 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000323816, ENST00000396115, ENST00000432992, ENST00000437099, ENST00000540214, ENST00000542249, ENST00000579158, ENST00000585266, ENST00000578655, ENST00000580865, ENST00000583882, | ENST00000530996, ENST00000542700, ENST00000609024, ENST00000347519, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 15 X 14 X 7=1470 | 4 X 3 X 3=36 |
| # samples | 17 | 4 | |
| ** MAII score | log2(17/1470*10)=-3.11220950358603 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(4/36*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: GAS7 [Title/Abstract] AND CHMP4A [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | GAS7(9923094)-CHMP4A(24681035), # samples:2 | ||
| Anticipated loss of major functional domain due to fusion event. | GAS7-CHMP4A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. GAS7-CHMP4A seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. GAS7-CHMP4A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. GAS7-CHMP4A seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. GAS7-CHMP4A seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. GAS7-CHMP4A seems lost the major protein functional domain in Tgene partner, which is a essential gene due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | CHMP4A | GO:0039702 | viral budding via host ESCRT complex | 24878737 |
| Tgene | CHMP4A | GO:0051258 | protein polymerization | 18209100 |
| Fusion gene breakpoints across GAS7 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across CHMP4A (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | GBM | TCGA-06-2562-01A | GAS7 | chr17 | 9923094 | - | CHMP4A | chr14 | 24681035 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000437099 | GAS7 | chr17 | 9923094 | - | ENST00000347519 | CHMP4A | chr14 | 24681035 | - | 1189 | 257 | 145 | 894 | 249 |
| ENST00000323816 | GAS7 | chr17 | 9923094 | - | ENST00000347519 | CHMP4A | chr14 | 24681035 | - | 1219 | 287 | 145 | 924 | 259 |
| ENST00000432992 | GAS7 | chr17 | 9923094 | - | ENST00000347519 | CHMP4A | chr14 | 24681035 | - | 1397 | 465 | 80 | 1102 | 340 |
| ENST00000585266 | GAS7 | chr17 | 9923094 | - | ENST00000347519 | CHMP4A | chr14 | 24681035 | - | 1367 | 435 | 308 | 1072 | 254 |
| ENST00000396115 | GAS7 | chr17 | 9923094 | - | ENST00000347519 | CHMP4A | chr14 | 24681035 | - | 1189 | 257 | 145 | 894 | 249 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000437099 | ENST00000347519 | GAS7 | chr17 | 9923094 | - | CHMP4A | chr14 | 24681035 | - | 0.001875288 | 0.99812466 |
| ENST00000323816 | ENST00000347519 | GAS7 | chr17 | 9923094 | - | CHMP4A | chr14 | 24681035 | - | 0.002192571 | 0.99780744 |
| ENST00000432992 | ENST00000347519 | GAS7 | chr17 | 9923094 | - | CHMP4A | chr14 | 24681035 | - | 0.005275761 | 0.9947242 |
| ENST00000585266 | ENST00000347519 | GAS7 | chr17 | 9923094 | - | CHMP4A | chr14 | 24681035 | - | 0.001920475 | 0.99807954 |
| ENST00000396115 | ENST00000347519 | GAS7 | chr17 | 9923094 | - | CHMP4A | chr14 | 24681035 | - | 0.001875288 | 0.99812466 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >32554_32554_1_GAS7-CHMP4A_GAS7_chr17_9923094_ENST00000323816_CHMP4A_chr14_24681035_ENST00000347519_length(amino acids)=259AA_BP=47 MNARRAMKPGMVPPPPGEESQTVILPPGWQSYLSPQGRRYYVNTTTNGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKIQQELQTAKK YGTKNKRAALQALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEVLRTMELAAQSMKKAYQDMDIDKVDELMTDITEQQEVAQQIS -------------------------------------------------------------- >32554_32554_2_GAS7-CHMP4A_GAS7_chr17_9923094_ENST00000396115_CHMP4A_chr14_24681035_ENST00000347519_length(amino acids)=249AA_BP=37 MVPPPPGEESQTVILPPGWQSYLSPQGRRYYVNTTTNGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKIQQELQTAKKYGTKNKRAAL QALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEVLRTMELAAQSMKKAYQDMDIDKVDELMTDITEQQEVAQQISDAISRPMGFG -------------------------------------------------------------- >32554_32554_3_GAS7-CHMP4A_GAS7_chr17_9923094_ENST00000432992_CHMP4A_chr14_24681035_ENST00000347519_length(amino acids)=340AA_BP=128 MGPRGAAAQPVGLGGNAGCAVNPGAKAMSGARCRTLYPFSGERHGQGLRFAAGELITLLQVPDGGWWEGEKEDGLRGWFPASYVQLLEKP GMVPPPPGEESQTVILPPGWQSYLSPQGRRYYVNTTTNGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKIQQELQTAKKYGTKNKRAA LQALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEVLRTMELAAQSMKKAYQDMDIDKVDELMTDITEQQEVAQQISDAISRPMGF -------------------------------------------------------------- >32554_32554_4_GAS7-CHMP4A_GAS7_chr17_9923094_ENST00000437099_CHMP4A_chr14_24681035_ENST00000347519_length(amino acids)=249AA_BP=37 MVPPPPGEESQTVILPPGWQSYLSPQGRRYYVNTTTNGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKIQQELQTAKKYGTKNKRAAL QALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEVLRTMELAAQSMKKAYQDMDIDKVDELMTDITEQQEVAQQISDAISRPMGFG -------------------------------------------------------------- >32554_32554_5_GAS7-CHMP4A_GAS7_chr17_9923094_ENST00000585266_CHMP4A_chr14_24681035_ENST00000347519_length(amino acids)=254AA_BP=42 MMKPGMVPPPPGEESQTVILPPGWQSYLSPQGRRYYVNTTTNGKKEKGPTPEEAIQKLKETEKILIKKQEFLEQKIQQELQTAKKYGTKN KRAALQALRRKKRFEQQLAQTDGTLSTLEFQREAIENATTNAEVLRTMELAAQSMKKAYQDMDIDKVDELMTDITEQQEVAQQISDAISR -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr17:9923094/chr14:24681035) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| GAS7 | CHMP4A |
| FUNCTION: May play a role in promoting maturation and morphological differentiation of cerebellar neurons. | FUNCTION: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4A filaments can promote or stabilize negative curvature and outward budding. Via its interaction with PDCD6IP involved in HIV-1 p6- and p9-dependent virus release. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). {ECO:0000269|PubMed:12860994, ECO:0000269|PubMed:14505569, ECO:0000269|PubMed:14519844, ECO:0000269|PubMed:14583093, ECO:0000269|PubMed:18209100, ECO:0000269|PubMed:22660413}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000432992 | - | 2 | 14 | 67_70 | 101.33333333333333 | 477.0 | Compositional bias | Note=Poly-Pro |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000432992 | - | 2 | 14 | 1_62 | 101.33333333333333 | 477.0 | Domain | SH3 |
| Tgene | CHMP4A | chr17:9923094 | chr14:24681035 | ENST00000347519 | 0 | 6 | 155_180 | 53.333333333333336 | 266.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | CHMP4A | chr17:9923094 | chr14:24681035 | ENST00000609024 | 0 | 6 | 155_180 | 10.333333333333334 | 223.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | CHMP4A | chr17:9923094 | chr14:24681035 | ENST00000609024 | 0 | 6 | 20_105 | 10.333333333333334 | 223.0 | Coiled coil | Ontology_term=ECO:0000255 |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000323816 | - | 3 | 15 | 309_419 | 41.333333333333336 | 417.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000396115 | - | 2 | 7 | 309_419 | 37.333333333333336 | 182.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000432992 | - | 2 | 14 | 309_419 | 101.33333333333333 | 477.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000437099 | - | 2 | 14 | 309_419 | 37.333333333333336 | 413.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000580865 | - | 1 | 10 | 309_419 | 0 | 337.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000585266 | - | 2 | 14 | 309_419 | 41.333333333333336 | 417.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000323816 | - | 3 | 15 | 67_70 | 41.333333333333336 | 417.0 | Compositional bias | Note=Poly-Pro |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000396115 | - | 2 | 7 | 67_70 | 37.333333333333336 | 182.0 | Compositional bias | Note=Poly-Pro |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000437099 | - | 2 | 14 | 67_70 | 37.333333333333336 | 413.0 | Compositional bias | Note=Poly-Pro |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000580865 | - | 1 | 10 | 67_70 | 0 | 337.0 | Compositional bias | Note=Poly-Pro |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000585266 | - | 2 | 14 | 67_70 | 41.333333333333336 | 417.0 | Compositional bias | Note=Poly-Pro |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000323816 | - | 3 | 15 | 196_456 | 41.333333333333336 | 417.0 | Domain | F-BAR |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000323816 | - | 3 | 15 | 1_62 | 41.333333333333336 | 417.0 | Domain | SH3 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000323816 | - | 3 | 15 | 77_110 | 41.333333333333336 | 417.0 | Domain | WW |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000396115 | - | 2 | 7 | 196_456 | 37.333333333333336 | 182.0 | Domain | F-BAR |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000396115 | - | 2 | 7 | 1_62 | 37.333333333333336 | 182.0 | Domain | SH3 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000396115 | - | 2 | 7 | 77_110 | 37.333333333333336 | 182.0 | Domain | WW |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000432992 | - | 2 | 14 | 196_456 | 101.33333333333333 | 477.0 | Domain | F-BAR |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000432992 | - | 2 | 14 | 77_110 | 101.33333333333333 | 477.0 | Domain | WW |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000437099 | - | 2 | 14 | 196_456 | 37.333333333333336 | 413.0 | Domain | F-BAR |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000437099 | - | 2 | 14 | 1_62 | 37.333333333333336 | 413.0 | Domain | SH3 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000437099 | - | 2 | 14 | 77_110 | 37.333333333333336 | 413.0 | Domain | WW |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000580865 | - | 1 | 10 | 196_456 | 0 | 337.0 | Domain | F-BAR |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000580865 | - | 1 | 10 | 1_62 | 0 | 337.0 | Domain | SH3 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000580865 | - | 1 | 10 | 77_110 | 0 | 337.0 | Domain | WW |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000585266 | - | 2 | 14 | 196_456 | 41.333333333333336 | 417.0 | Domain | F-BAR |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000585266 | - | 2 | 14 | 1_62 | 41.333333333333336 | 417.0 | Domain | SH3 |
| Hgene | GAS7 | chr17:9923094 | chr14:24681035 | ENST00000585266 | - | 2 | 14 | 77_110 | 41.333333333333336 | 417.0 | Domain | WW |
| Tgene | CHMP4A | chr17:9923094 | chr14:24681035 | ENST00000347519 | 0 | 6 | 20_105 | 53.333333333333336 | 266.0 | Coiled coil | Ontology_term=ECO:0000255 |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| GAS7 | PACSIN1, GAS7, ATXN1L, CREB3L2, HNRNPU, KHDRBS1, SF1, SF3A1, SFPQ, RPLP0, ABI1, CYFIP1, CYFIP2, DIAPH1, FMNL1, WAS, WASF2, APBB1IP, NCKAP1, NCKAP1L, GAPDH, WDYHV1, NCKIPSD, CDC37, LSM4, YIF1A, MCRS1, MYBPC2, BRD4, DDX58, |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| GAS7 |  |
| CHMP4A |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| Tgene | CHMP4A | chr17:9923094 | chr14:24681035 | ENST00000347519 | 0 | 6 | 1_116 | 53.333333333333336 | 266.0 | phosphoinosides | |
| Tgene | CHMP4A | chr17:9923094 | chr14:24681035 | ENST00000609024 | 0 | 6 | 1_116 | 10.333333333333334 | 223.0 | phosphoinosides |
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Related Drugs to GAS7-CHMP4A |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to GAS7-CHMP4A |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | GAS7 | C0028754 | Obesity | 1 | CTD_human |
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