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Center for Computational Systems Medicine level1
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:ANAPC5-LTA4H

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ANAPC5-LTA4H
FusionPDB ID: 4300
FusionGDB2.0 ID: 4300
HgeneTgene
Gene symbol

ANAPC5

LTA4H

Gene ID

51433

4048

Gene nameanaphase promoting complex subunit 5leukotriene A4 hydrolase
SynonymsAPC5-
Cytomap

12q24.31

12q23.1

Type of geneprotein-codingprotein-coding
Descriptionanaphase-promoting complex subunit 5cyclosome subunit 5leukotriene A-4 hydrolaseLTA-4 hydrolasetesticular secretory protein Li 27
Modification date2020031320200313
UniProtAcc

Q9UJX4

P09960

Ensembl transtripts involved in fusion geneENST idsENST00000261819, ENST00000344395, 
ENST00000441917, ENST00000535482, 
ENST00000541887, ENST00000544314, 
ENST00000536366, 		ENST00000548375, 
ENST00000228740, ENST00000413268, 
ENST00000552789, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score9 X 11 X 6=5949 X 9 X 6=486
# samples 118
** MAII scorelog2(11/594*10)=-2.43295940727611
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(8/486*10)=-2.60288440871842
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: ANAPC5 [Title/Abstract] AND LTA4H [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ANAPC5(121756080)-LTA4H(96402926), # samples:1
Anticipated loss of major functional domain due to fusion event.ANAPC5-LTA4H seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ANAPC5-LTA4H seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ANAPC5-LTA4H seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ANAPC5-LTA4H seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ANAPC5-LTA4H seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
ANAPC5-LTA4H seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF.
ANAPC5-LTA4H seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneANAPC5

GO:0070979

protein K11-linked ubiquitination

18485873

TgeneLTA4H

GO:0019370

leukotriene biosynthetic process

9774412|15078870

TgeneLTA4H

GO:0043171

peptide catabolic process

9774412|15078870|18804029


check buttonFusion gene breakpoints across ANAPC5 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across LTA4H (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4SARCTCGA-DX-A3LS-01AANAPC5chr12

121756080

-LTA4Hchr12

96402926

-


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000441917ANAPC5chr12121756080-ENST00000228740LTA4Hchr1296402926-22671649171654545
ENST00000441917ANAPC5chr12121756080-ENST00000552789LTA4Hchr1296402926-22651649171654545
ENST00000441917ANAPC5chr12121756080-ENST00000413268LTA4Hchr1296402926-21051649171654545

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000441917ENST00000228740ANAPC5chr12121756080-LTA4Hchr1296402926-0.0025187490.9974813
ENST00000441917ENST00000552789ANAPC5chr12121756080-LTA4Hchr1296402926-0.0025306630.9974693
ENST00000441917ENST00000413268ANAPC5chr12121756080-LTA4Hchr1296402926-0.0035545120.9964455

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>4300_4300_1_ANAPC5-LTA4H_ANAPC5_chr12_121756080_ENST00000441917_LTA4H_chr12_96402926_ENST00000228740_length(amino acids)=545AA_BP=
MSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQN
GEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAH
YLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCL
SWLYVLGQKRSDSYVLLEHSVKKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLS
MNSLEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITA
LNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNL

--------------------------------------------------------------

>4300_4300_2_ANAPC5-LTA4H_ANAPC5_chr12_121756080_ENST00000441917_LTA4H_chr12_96402926_ENST00000413268_length(amino acids)=545AA_BP=
MSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQN
GEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAH
YLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCL
SWLYVLGQKRSDSYVLLEHSVKKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLS
MNSLEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITA
LNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNL

--------------------------------------------------------------

>4300_4300_3_ANAPC5-LTA4H_ANAPC5_chr12_121756080_ENST00000441917_LTA4H_chr12_96402926_ENST00000552789_length(amino acids)=545AA_BP=
MSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQN
GEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAH
YLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCL
SWLYVLGQKRSDSYVLLEHSVKKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLS
MNSLEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITA
LNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNL

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr12:121756080/chr12:96402926)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ANAPC5

Q9UJX4

LTA4H

P09960

FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. {ECO:0000269|PubMed:18485873}.FUNCTION: Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4) (PubMed:11917124, PubMed:12207002, PubMed:15078870, PubMed:18804029, PubMed:1897988, PubMed:1975494, PubMed:2244921). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (PubMed:20813919, PubMed:18804029). In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed:20813919, PubMed:24591641). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (PubMed:21206090). {ECO:0000269|PubMed:11917124, ECO:0000269|PubMed:12207002, ECO:0000269|PubMed:15078870, ECO:0000269|PubMed:18804029, ECO:0000269|PubMed:1897988, ECO:0000269|PubMed:1975494, ECO:0000269|PubMed:20813919, ECO:0000269|PubMed:21206090, ECO:0000269|PubMed:2244921, ECO:0000269|PubMed:24591641}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517209_249631.0756.0RepeatNote=TPR 1
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517250_300631.0756.0RepeatNote=TPR 2
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517301_337631.0756.0RepeatNote=TPR 3
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517338_378631.0756.0RepeatNote=TPR 4
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517379_418631.0756.0RepeatNote=TPR 5
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517419_466631.0756.0RepeatNote=TPR 6
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517467_500631.0756.0RepeatNote=TPR 7
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517501_540631.0756.0RepeatNote=TPR 8
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517541_580631.0756.0RepeatNote=TPR 9
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517581_620631.0756.0RepeatNote=TPR 10
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315209_249519.0644.0RepeatNote=TPR 1
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315250_300519.0644.0RepeatNote=TPR 2
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315301_337519.0644.0RepeatNote=TPR 3
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315338_378519.0644.0RepeatNote=TPR 4
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315379_418519.0644.0RepeatNote=TPR 5
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315419_466519.0644.0RepeatNote=TPR 6
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315467_500519.0644.0RepeatNote=TPR 7
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416209_249519.0644.0RepeatNote=TPR 1
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416250_300519.0644.0RepeatNote=TPR 2
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416301_337519.0644.0RepeatNote=TPR 3
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416338_378519.0644.0RepeatNote=TPR 4
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416379_418519.0644.0RepeatNote=TPR 5
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416419_466519.0644.0RepeatNote=TPR 6
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416467_500519.0644.0RepeatNote=TPR 7
TgeneLTA4Hchr12:121756080chr12:96402926ENST000002287401319564_566459.6666666666667612.0RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000004132681318564_566435.6666666666667489.6666666666667RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000005527891319564_566435.6666666666667588.0RegionSubstrate binding

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517621_660631.0756.0RepeatNote=TPR 11
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517661_696631.0756.0RepeatNote=TPR 12
HgeneANAPC5chr12:121756080chr12:96402926ENST00000261819-1517697_736631.0756.0RepeatNote=TPR 13
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315501_540519.0644.0RepeatNote=TPR 8
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315541_580519.0644.0RepeatNote=TPR 9
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315581_620519.0644.0RepeatNote=TPR 10
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315621_660519.0644.0RepeatNote=TPR 11
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315661_696519.0644.0RepeatNote=TPR 12
HgeneANAPC5chr12:121756080chr12:96402926ENST00000344395-1315697_736519.0644.0RepeatNote=TPR 13
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416501_540519.0644.0RepeatNote=TPR 8
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416541_580519.0644.0RepeatNote=TPR 9
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416581_620519.0644.0RepeatNote=TPR 10
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416621_660519.0644.0RepeatNote=TPR 11
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416661_696519.0644.0RepeatNote=TPR 12
HgeneANAPC5chr12:121756080chr12:96402926ENST00000441917-1416697_736519.0644.0RepeatNote=TPR 13
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15209_2490.0220.0RepeatNote=TPR 1
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15250_3000.0220.0RepeatNote=TPR 2
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15301_3370.0220.0RepeatNote=TPR 3
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15338_3780.0220.0RepeatNote=TPR 4
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15379_4180.0220.0RepeatNote=TPR 5
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15419_4660.0220.0RepeatNote=TPR 6
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15467_5000.0220.0RepeatNote=TPR 7
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15501_5400.0220.0RepeatNote=TPR 8
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15541_5800.0220.0RepeatNote=TPR 9
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15581_6200.0220.0RepeatNote=TPR 10
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15621_6600.0220.0RepeatNote=TPR 11
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15661_6960.0220.0RepeatNote=TPR 12
HgeneANAPC5chr12:121756080chr12:96402926ENST00000536366-15697_7360.0220.0RepeatNote=TPR 13
TgeneLTA4Hchr12:121756080chr12:96402926ENST000002287401319135_137459.6666666666667612.0RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000002287401319267_272459.6666666666667612.0RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000002287401319362_365459.6666666666667612.0RegionInhibitor ARM1 binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000004132681318135_137435.6666666666667489.6666666666667RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000004132681318267_272435.6666666666667489.6666666666667RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000004132681318362_365435.6666666666667489.6666666666667RegionInhibitor ARM1 binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000005527891319135_137435.6666666666667588.0RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000005527891319267_272435.6666666666667588.0RegionSubstrate binding
TgeneLTA4Hchr12:121756080chr12:96402926ENST000005527891319362_365435.6666666666667588.0RegionInhibitor ARM1 binding


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
ANAPC5
LTA4H


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to ANAPC5-LTA4H


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to ANAPC5-LTA4H


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource