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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:MAX-VTI1B

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: MAX-VTI1B
	FusionPDB ID: 51941
	FusionGDB2.0 ID: 51941
		Hgene	Tgene
	Gene symbol	MAX	VTI1B
	Gene ID	4149	10490
	Gene name	MYC associated factor X	vesicle transport through interaction with t-SNAREs 1B
	Synonyms	bHLHd4	VTI1\|VTI1-LIKE\|VTI1L\|VTI2\|v-SNARE\|vti1-rp1
	Cytomap	14q23.3	14q24.1
	Type of gene	protein-coding	protein-coding
	Description	protein maxclass D basic helix-loop-helix protein 4	vesicle transport through interaction with t-SNAREs homolog 1Bvesicle transport v-SNARE protein Vti1-like 1vesicle-associated soluble NSF attachment protein receptor
	Modification date	20200313	20200313
	UniProtAcc	P61244	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000557277, ENST00000246163, ENST00000284165, ENST00000341653, ENST00000358402, ENST00000358664, ENST00000555667, ENST00000556443, ENST00000556979, ENST00000557746, ENST00000555419, ENST00000555932,	ENST00000554659,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	6 X 4 X 6=144	6 X 6 X 4=144
	# samples	6	8
	** MAII score	log2(6/144*10)=-1.26303440583379 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(8/144*10)=-0.84799690655495 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: MAX [Title/Abstract] AND VTI1B [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	MAX(65560425)-VTI1B(68126639), # samples:1
Anticipated loss of major functional domain due to fusion event.	MAX-VTI1B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MAX-VTI1B seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MAX-VTI1B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MAX-VTI1B seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	MAX	GO:0000122	negative regulation of transcription by RNA polymerase II	8521822
Tgene	VTI1B	GO:1903076	regulation of protein localization to plasma membrane	18570918

Fusion gene breakpoints across MAX (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across VTI1B (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	STAD	TCGA-D7-A74A	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000341653	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5054	219	48	743	231
ENST00000358402	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5144	309	165	833	222
ENST00000284165	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5155	320	149	844	231
ENST00000358664	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5137	302	131	826	231
ENST00000556979	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5184	349	178	873	231
ENST00000555667	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5157	322	178	846	222
ENST00000557746	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5157	322	178	846	222
ENST00000556443	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5157	322	178	846	222
ENST00000246163	MAX	chr14	65560425	-	ENST00000554659	VTI1B	chr14	68126639	-	5176	341	170	865	231

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000341653	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000700421	0.9992995
ENST00000358402	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000811474	0.9991885
ENST00000284165	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000712618	0.9992874
ENST00000358664	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000705944	0.99929404
ENST00000556979	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000694057	0.99930596
ENST00000555667	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000810648	0.9991893
ENST00000557746	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000810648	0.9991893
ENST00000556443	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000810648	0.9991893
ENST00000246163	ENST00000554659	MAX	chr14	65560425	-	VTI1B	chr14	68126639	-	0.000690395	0.99930966

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>51941_51941_1_MAX-VTI1B_MAX_chr14_65560425_ENST00000246163_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=231AA_BP=57
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKL
HREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRL

--------------------------------------------------------------

>51941_51941_2_MAX-VTI1B_MAX_chr14_65560425_ENST00000284165_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=231AA_BP=57
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKL
HREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRL

--------------------------------------------------------------

>51941_51941_3_MAX-VTI1B_MAX_chr14_65560425_ENST00000341653_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=231AA_BP=57
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKL
HREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRL

--------------------------------------------------------------

>51941_51941_4_MAX-VTI1B_MAX_chr14_65560425_ENST00000358402_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=222AA_BP=48
MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPL
TATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSK

--------------------------------------------------------------

>51941_51941_5_MAX-VTI1B_MAX_chr14_65560425_ENST00000358664_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=231AA_BP=57
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKL
HREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRL

--------------------------------------------------------------

>51941_51941_6_MAX-VTI1B_MAX_chr14_65560425_ENST00000555667_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=222AA_BP=48
MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPL
TATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSK

--------------------------------------------------------------

>51941_51941_7_MAX-VTI1B_MAX_chr14_65560425_ENST00000556443_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=222AA_BP=48
MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPL
TATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSK

--------------------------------------------------------------

>51941_51941_8_MAX-VTI1B_MAX_chr14_65560425_ENST00000556979_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=231AA_BP=57
MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKL
HREVRSTPLTATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRL

--------------------------------------------------------------

>51941_51941_9_MAX-VTI1B_MAX_chr14_65560425_ENST00000557746_VTI1B_chr14_68126639_ENST00000554659_length(amino acids)=222AA_BP=48
MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKLAEMEEELRYAPLSFRNPMMSKLRNYRKDLAKLHREVRSTPL
TATPGGRGDMKYGIYAVENEHMNRLQSQRAMLLQGTESLNRATQSIERSHRIATETDQIGSEIIEELGEQRDQLERTKSRLVNTSENLSK

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr14:65560425/chr14:68126639)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
MAX P61244	.
FUNCTION: Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. Represses MYC transcriptional activity from E-box elements. {ECO:0000269\|PubMed:26070438}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

VTI1B

chr14:65560425

chr14:68126639

ENST00000554659

161_198

58.0

233.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

VTI1B

chr14:65560425

chr14:68126639

ENST00000554659

230_232

58.0

233.0

Topological domain

Vesicular

Tgene

VTI1B

chr14:65560425

chr14:68126639

ENST00000554659

209_229

58.0

233.0

Transmembrane

Helical%3B Anchor for type IV membrane protein

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000246163

23_74

57.0

97.0

Domain

bHLH

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000284165

23_74

57.0

135.0

Domain

bHLH

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000341653

23_74

57.0

84.0

Domain

bHLH

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000358402

23_74

48.0

152.0

Domain

bHLH

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000358664

23_74

57.0

161.0

Domain

bHLH

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000556979

23_74

57.0

104.0

Domain

bHLH

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000246163

152_156

57.0

97.0

Motif

Note=Nuclear localization signal

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000284165

152_156

57.0

135.0

Motif

Note=Nuclear localization signal

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000341653

152_156

57.0

84.0

Motif

Note=Nuclear localization signal

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000358402

152_156

48.0

152.0

Motif

Note=Nuclear localization signal

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000358664

152_156

57.0

161.0

Motif

Note=Nuclear localization signal

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000556979

152_156

57.0

104.0

Motif

Note=Nuclear localization signal

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000246163

81_102

57.0

97.0

Region

Note=Leucine-zipper

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000284165

81_102

57.0

135.0

Region

Note=Leucine-zipper

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000341653

81_102

57.0

84.0

Region

Note=Leucine-zipper

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000358402

81_102

48.0

152.0

Region

Note=Leucine-zipper

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000358664

81_102

57.0

161.0

Region

Note=Leucine-zipper

Hgene

MAX

chr14:65560425

chr14:68126639

ENST00000556979

81_102

57.0

104.0

Region

Note=Leucine-zipper

Tgene

VTI1B

chr14:65560425

chr14:68126639

ENST00000554659

35_98

58.0

233.0

Coiled coil

Ontology_term=ECO:0000255

Tgene

VTI1B

chr14:65560425

chr14:68126639

ENST00000554659

2_208

58.0

233.0

Topological domain

Cytoplasmic

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
MAX
VTI1B

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	VTI1B	chr14:65560425	chr14:68126639	ENST00000554659		1	6	2_23	58.0	233.0	CLINT1

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Related Drugs to MAX-VTI1B

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to MAX-VTI1B

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source