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Fusion Protein:MSMB-KLK3 |
Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: MSMB-KLK3 | FusionPDB ID: 55377 | FusionGDB2.0 ID: 55377 | Hgene | Tgene | Gene symbol | MSMB | KLK3 | Gene ID | 4477 | 3818 |
Gene name | microseminoprotein beta | kallikrein B1 | |
Synonyms | HPC13|IGBF|MSP|MSPB|PN44|PRPS|PSP|PSP-94|PSP57|PSP94 | KLK3|PKK|PKKD|PPK | |
Cytomap | 10q11.22 | 4q35.2 | |
Type of gene | protein-coding | protein-coding | |
Description | beta-microseminoproteinimmunoglobulin binding factorprostate secreted seminal plasma proteinprostate secretory protein of 94 amino acidsseminal plasma beta-inhibin | plasma kallikreinkallikrein B, plasma (Fletcher factor) 1kininogeninplasma prekallikrein | |
Modification date | 20200313 | 20200320 | |
UniProtAcc | P08118 | P07288 | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000298239, ENST00000358559, ENST00000474170, | ENST00000360617, ENST00000593997, ENST00000595952, ENST00000597483, ENST00000326003, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 15 X 7 X 2=210 | 30 X 32 X 3=2880 |
# samples | 15 | 37 | |
** MAII score | log2(15/210*10)=-0.485426827170242 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(37/2880*10)=-2.96047163581336 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context (manual curation of fusion genes in FusionPDB) | PubMed: MSMB [Title/Abstract] AND KLK3 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | MSMB(51562517)-KLK3(51361285), # samples:2 KLK3(51361836)-MSMB(51562326), # samples:1 | ||
Anticipated loss of major functional domain due to fusion event. | KLK3-MSMB seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. KLK3-MSMB seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MSMB-KLK3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MSMB-KLK3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. MSMB-KLK3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MSMB-KLK3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. MSMB-KLK3 seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF. MSMB-KLK3 seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Tgene | KLK3 | GO:0031639 | plasminogen activation | 89876 |
Tgene | KLK3 | GO:0051919 | positive regulation of fibrinolysis | 89876 |
![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
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![]() * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | PRAD | TCGA-J4-A67O-01A | MSMB | chr10 | 51562517 | - | KLK3 | chr19 | 51361285 | + |
ChimerDB4 | PRAD | TCGA-J9-A52B-01A | MSMB | chr10 | 51562517 | - | KLK3 | chr19 | 51361285 | + |
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Fusion ORF Analysis |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000298239 | MSMB | chr10 | 51562517 | - | ENST00000326003 | KLK3 | chr19 | 51361285 | + | 1598 | 382 | 371 | 961 | 196 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000298239 | ENST00000326003 | MSMB | chr10 | 51562517 | - | KLK3 | chr19 | 51361285 | + | 0.09274833 | 0.9072517 |
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Fusion Amino Acid Sequences |
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>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >55377_55377_1_MSMB-KLK3_MSMB_chr10_51562517_ENST00000298239_KLK3_chr19_51361285_ENST00000326003_length(amino acids)=196AA_BP=4 MSKHKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYAS GWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTK -------------------------------------------------------------- |
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Fusion Protein Functional Features |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:51562517/chr19:51361285) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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Hgene | Tgene |
MSMB | KLK3 |
FUNCTION: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. |
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- Retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Tgene | KLK3 | chr10:51562517 | chr19:51361285 | ENST00000595952 | 0 | 5 | 25_258 | 0 | 219.0 | Domain | Peptidase S1 |
- Not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Tgene | KLK3 | chr10:51562517 | chr19:51361285 | ENST00000326003 | 1 | 5 | 25_258 | 68.66666666666667 | 262.0 | Domain | Peptidase S1 | |
Tgene | KLK3 | chr10:51562517 | chr19:51361285 | ENST00000360617 | 1 | 5 | 25_258 | 68.66666666666667 | 239.0 | Domain | Peptidase S1 | |
Tgene | KLK3 | chr10:51562517 | chr19:51361285 | ENST00000593997 | 1 | 4 | 25_258 | 68.66666666666667 | 228.0 | Domain | Peptidase S1 |
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Fusion Protein Structures |
![]() * Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
Fusion protein PDB link (fusion AA seq ID in FusionPDB) | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | AA seq | Len(AA seq) |
PDB file >>>254_MSMB_51562517_KLK3_51361285_ranked_0.pdb | MSMB | 51562517 | 51562517 | ENST00000326003 | KLK3 | chr19 | 51361285 | + | MSKHKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYAS GWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTK | 196 |
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pLDDT score distribution |
![]() * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
MSMB_pLDDT.png![]() |
KLK3_pLDDT.png![]() |
![]() * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
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Ramachandran Plot of Fusion Protein Structure |
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Fusion AA seq ID in FusionPDB and their Ramachandran plots |
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Fusion Protein-Protein Interaction |
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Gene | PPI interactors |
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Gene | STRING network |
MSMB | |
KLK3 |
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Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
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Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to MSMB-KLK3 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to MSMB-KLK3 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |