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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:NME7-KMO

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: NME7-KMO
	FusionPDB ID: 59434
	FusionGDB2.0 ID: 59434
		Hgene	Tgene
	Gene symbol	NME7	KMO
	Gene ID	29922	8564
	Gene name	NME/NM23 family member 7	kynurenine 3-monooxygenase
	Synonyms	CFAP67\|MN23H7\|NDK 7\|NDK7\|nm23-H7	dJ317G22.1
	Cytomap	1q24.2	1q43
	Type of gene	protein-coding	protein-coding
	Description	nucleoside diphosphate kinase 7NDP kinase 7cilia and flagella associated protein 67non-metastatic cells 7, protein expressed in (nucleoside-diphosphate kinase)	kynurenine 3-monooxygenasekynurenine 3-hydroxylase
	Modification date	20200327	20200313
	UniProtAcc	Q9Y5B8	O15229
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000367811, ENST00000472647, ENST00000469474,	ENST00000366557, ENST00000484628, ENST00000366558, ENST00000366559,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	8 X 5 X 5=200	3 X 3 X 3=27
	# samples	8	4
	** MAII score	log2(8/200*10)=-1.32192809488736 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(4/27*10)=0.567040592723894 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: NME7 [Title/Abstract] AND KMO [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	NME7(169199956)-KMO(241749981), # samples:2
Anticipated loss of major functional domain due to fusion event.	NME7-KMO seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NME7-KMO seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NME7-KMO seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. NME7-KMO seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF. NME7-KMO seems lost the major protein functional domain in Tgene partner, which is a cell metabolism gene due to the frame-shifted ORF. NME7-KMO seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	KMO	GO:0009651	response to salt stress	10672018
Tgene	KMO	GO:0019674	NAD metabolic process	23575632\|29429898
Tgene	KMO	GO:0070189	kynurenine metabolic process	10672018\|26752518\|29208702\|29429898

Fusion gene breakpoints across NME7 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across KMO (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	PRAD	TCGA-VP-A87D-01A	NME7	chr1	169199956	-	KMO	chr1	241749981	+

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst

Tenst

Hgene

Hchr

Hbp

Hstrand

Tgene

Tchr

Tbp

Tstrand

Seq length
(transcript)

BP loci
(transcript)

Predicted start
(transcript)

Predicted stop
(transcript)

Seq length
(amino acids)

ENST00000367811

NME7

chr1

169199956

ENST00000366558

KMO

chr1

241749981

1847

1247

257

1711

484

ENST00000367811

NME7

chr1

169199956

ENST00000366559

KMO

chr1

241749981

5240

1247

257

1750

497

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000367811	ENST00000366558	NME7	chr1	169199956	-	KMO	chr1	241749981	+	0.001079208	0.9989208
ENST00000367811	ENST00000366559	NME7	chr1	169199956	-	KMO	chr1	241749981	+	0.000642388	0.99935764

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>59434_59434_1_NME7-KMO_NME7_chr1_169199956_ENST00000367811_KMO_chr1_241749981_ENST00000366558_length(amino acids)=484AA_BP=330
MNHSERFVFIAEWYDPNASLLRRYELLFYPGDGSVEMHDVKNHRTFLKRTKYDNLHLEDLFIGNKVNVFSRQLVLIDYGDQYTARQLGSR
KEKTLALIKPDAISKAGEIIEIINKAGFTITKLKMMMLSRKEALDFHVDHQSRPFFNELIQFITTGPIIAMEILRDDAICEWKRLLGPAN
SGVARTDASESIRALFGTDGIRNAAHGPDSFASAAREMELFFPSSGGCGPANTAKFTNCTCCIVKPHAVSEGLLGKILMAIRDAGFEISA
MQMFNMDRVNVEEFYEVYKGVVTEYHDMVTEMYSGPCVAMEIQQNNATKTFREFCGPADPGFEDCLVFDELMDKFSNDLSLCLPVFSRLR
IPDDHAISDLSMYNYIEKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKKVINKGLFFLGSLIAISSTYLLIHYMSPRSFLR

--------------------------------------------------------------

>59434_59434_2_NME7-KMO_NME7_chr1_169199956_ENST00000367811_KMO_chr1_241749981_ENST00000366559_length(amino acids)=497AA_BP=330
MNHSERFVFIAEWYDPNASLLRRYELLFYPGDGSVEMHDVKNHRTFLKRTKYDNLHLEDLFIGNKVNVFSRQLVLIDYGDQYTARQLGSR
KEKTLALIKPDAISKAGEIIEIINKAGFTITKLKMMMLSRKEALDFHVDHQSRPFFNELIQFITTGPIIAMEILRDDAICEWKRLLGPAN
SGVARTDASESIRALFGTDGIRNAAHGPDSFASAAREMELFFPSSGGCGPANTAKFTNCTCCIVKPHAVSEGLLGKILMAIRDAGFEISA
MQMFNMDRVNVEEFYEVYKGVVTEYHDMVTEMYSGPCVAMEIQQNNATKTFREFCGPADPGFEDCLVFDELMDKFSNDLSLCLPVFSRLR
IPDDHAISDLSMYNYIEMRAHVNSSWFIFQKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKKVINKGLFFLGSLIAISSTY

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:169199956/chr1:241749981)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
NME7 Q9Y5B8	KMO O15229
FUNCTION: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.	FUNCTION: Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn) (PubMed:29429898, PubMed:23575632, PubMed:26752518, PubMed:28604669, PubMed:29208702). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable). {ECO:0000269\|PubMed:23575632, ECO:0000269\|PubMed:26752518, ECO:0000269\|PubMed:28604669, ECO:0000269\|PubMed:29208702, ECO:0000269\|PubMed:29429898, ECO:0000305\|PubMed:12402501}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

NME7

chr1:169199956

chr1:241749981

ENST00000367811

3_91

330.0

377.0

Domain

DM10

Hgene

NME7

chr1:169199956

chr1:241749981

ENST00000472647

3_91

294.0

341.0

Domain

DM10

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366557

317_318

319.0

453.0

Nucleotide binding

FAD

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366558

317_318

319.0

474.0

Nucleotide binding

FAD

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366559

317_318

319.0

487.0

Nucleotide binding

FAD

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366557

385_404

319.0

453.0

Transmembrane

Helical

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366557

425_445

319.0

453.0

Transmembrane

Helical

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366558

385_404

319.0

474.0

Transmembrane

Helical

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366558

425_445

319.0

474.0

Transmembrane

Helical

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366559

385_404

319.0

487.0

Transmembrane

Helical

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366559

425_445

319.0

487.0

Transmembrane

Helical

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366557

37_40

319.0

453.0

Nucleotide binding

FAD

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366558

37_40

319.0

474.0

Nucleotide binding

FAD

Tgene

KMO

chr1:169199956

chr1:241749981

ENST00000366559

37_40

319.0

487.0

Nucleotide binding

FAD

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
NME7
KMO

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to NME7-KMO

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

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Related Diseases to NME7-KMO

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source