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Fusion Protein:NOV-ENPP2 |
Fusion Protein Summary |
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Fusion partner gene information | Fusion gene name: NOV-ENPP2 | FusionPDB ID: 59804 | FusionGDB2.0 ID: 59804 | Hgene | Tgene | Gene symbol | NOV | ENPP2 | Gene ID | 6134 | 5168 |
Gene name | ribosomal protein L10 | ectonucleotide pyrophosphatase/phosphodiesterase 2 | |
Synonyms | AUTSX5|DXS648|DXS648E|L10|MRXS35|NOV|QM | ATX|ATX-X|AUTOTAXIN|LysoPLD|NPP2|PD-IALPHA|PDNP2 | |
Cytomap | Xq28 | 8q24.12 | |
Type of gene | protein-coding | protein-coding | |
Description | 60S ribosomal protein L10Wilms tumor-related proteinlaminin receptor homologlarge ribosomal subunit protein uL16tumor suppressor QM | ectonucleotide pyrophosphatase/phosphodiesterase family member 2E-NPP 2autotaxin-textracellular lysophospholipase Dphosphodiesterase I/nucleotide pyrophosphatase 2plasma lysophospholipase D | |
Modification date | 20200313 | 20200313 | |
UniProtAcc | Q9UNW9 | Q13822 | |
Ensembl transtripts involved in fusion gene | ENST ids | ENST00000259526, | ENST00000075322, ENST00000518109, ENST00000522167, ENST00000522826, ENST00000259486, ENST00000427067, |
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 2 X 2 X 2=8 | 10 X 9 X 6=540 |
# samples | 2 | 11 | |
** MAII score | log2(2/8*10)=1.32192809488736 | log2(11/540*10)=-2.29545588352617 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
Context (manual curation of fusion genes in FusionPDB) | PubMed: NOV [Title/Abstract] AND ENPP2 [Title/Abstract] AND fusion [Title/Abstract] | ||
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | NOV(120431585)-ENPP2(120606100), # samples:5 | ||
Anticipated loss of major functional domain due to fusion event. | NOV-ENPP2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NOV-ENPP2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NOV-ENPP2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NOV-ENPP2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. |
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
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Partner | Gene | GO ID | GO term | PubMed ID |
Tgene | ENPP2 | GO:0009395 | phospholipid catabolic process | 15280042|26371182 |
Tgene | ENPP2 | GO:0030149 | sphingolipid catabolic process | 14500380 |
Tgene | ENPP2 | GO:0030334 | regulation of cell migration | 1733949 |
Tgene | ENPP2 | GO:0034638 | phosphatidylcholine catabolic process | 21240271 |
Tgene | ENPP2 | GO:0050731 | positive regulation of peptidyl-tyrosine phosphorylation | 18054784 |
![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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![]() * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
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![]() * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
ChimerDB4 | ESCA | TCGA-JY-A6FD-01A | NOV | chr8 | 120431585 | - | ENPP2 | chr8 | 120606100 | - |
ChimerDB4 | ESCA | TCGA-JY-A6FD-01A | NOV | chr8 | 120431585 | + | ENPP2 | chr8 | 120606100 | - |
ChimerDB4 | ESCA | TCGA-JY-A6FD | NOV | chr8 | 120431585 | + | ENPP2 | chr8 | 120606100 | - |
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Fusion ORF Analysis |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
ENST00000259526 | NOV | chr8 | 120431585 | + | ENST00000259486 | ENPP2 | chr8 | 120606100 | - | 3059 | 1004 | 8 | 2623 | 871 |
ENST00000259526 | NOV | chr8 | 120431585 | + | ENST00000427067 | ENPP2 | chr8 | 120606100 | - | 3134 | 1004 | 8 | 2698 | 896 |
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Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
ENST00000259526 | ENST00000259486 | NOV | chr8 | 120431585 | + | ENPP2 | chr8 | 120606100 | - | 0.001919771 | 0.99808025 |
ENST00000259526 | ENST00000427067 | NOV | chr8 | 120431585 | + | ENPP2 | chr8 | 120606100 | - | 0.001863173 | 0.99813676 |
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Fusion Amino Acid Sequences |
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>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >59804_59804_1_NOV-ENPP2_NOV_chr8_120431585_ENST00000259526_ENPP2_chr8_120606100_ENST00000259486_length(amino acids)=871AA_BP=332 MPWQPRGSFHFPTQPISPLLPALQPTGLCASQERAIKPVLGVIGKHRTRGKASSANLQRRKSRLVKARGESLSMQSVQSTSFCLRKQCLC LTFLLLHLLGQVAATQRCPPQCPGRCPATPPTCAPGVRAVLDGCSCCLVCARQRGESCSDLEPCDESSGLYCDRSADPSNQTGICTAVEG DNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGCVPRCQLDVLLPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGV EVSDSSVNCIEQTTEWTACSKSCGMGFSTRVTNRNRQCEMLKQTRLCMVRPCEQEPEQPTDKMTNPLREIDKIVGQLMDGLKQLKLHRCV NVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIE DIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLN HLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEERHLLYGRPAVLYRTRYDILYHTDFESGY SEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRV WNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDN -------------------------------------------------------------- >59804_59804_2_NOV-ENPP2_NOV_chr8_120431585_ENST00000259526_ENPP2_chr8_120606100_ENST00000427067_length(amino acids)=896AA_BP=332 MPWQPRGSFHFPTQPISPLLPALQPTGLCASQERAIKPVLGVIGKHRTRGKASSANLQRRKSRLVKARGESLSMQSVQSTSFCLRKQCLC LTFLLLHLLGQVAATQRCPPQCPGRCPATPPTCAPGVRAVLDGCSCCLVCARQRGESCSDLEPCDESSGLYCDRSADPSNQTGICTAVEG DNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGCVPRCQLDVLLPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGV EVSDSSVNCIEQTTEWTACSKSCGMGFSTRVTNRNRQCEMLKQTRLCMVRPCEQEPEQPTDKMTNPLREIDKIVGQLMDGLKQLKLHRCV NVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIE DIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLN HLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEAETRKFRGSRNENKENINGNFEPRKERHL LYGRPAVLYRTRYDILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLS SSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLD -------------------------------------------------------------- |
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Fusion Protein Functional Features |
![]() Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr8:120431585/chr8:120606100) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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Hgene | Tgene |
NOV | ENPP2 |
FUNCTION: May regulate RNA splicing or metabolism in a specific subset of developing neurons (By similarity). Binds single strand RNA. {ECO:0000250}. | FUNCTION: Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931, PubMed:14500380, PubMed:12354767,). Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931, PubMed:14500380). Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor (PubMed:1733949, PubMed:11559573). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed:21393252). {ECO:0000269|PubMed:11559573, ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:12354767, ECO:0000269|PubMed:14500380, ECO:0000269|PubMed:15769751, ECO:0000269|PubMed:1733949, ECO:0000269|PubMed:21240271, ECO:0000269|PubMed:21393252, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000305|PubMed:15700135}. |
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- Retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Hgene | NOV | chr8:120431585 | chr8:120606100 | ENST00000259526 | + | 4 | 5 | 108_174 | 259.0 | 358.0 | Domain | VWFC |
Hgene | NOV | chr8:120431585 | chr8:120606100 | ENST00000259526 | + | 4 | 5 | 205_250 | 259.0 | 358.0 | Domain | TSP type-1 |
Hgene | NOV | chr8:120431585 | chr8:120606100 | ENST00000259526 | + | 4 | 5 | 32_105 | 259.0 | 358.0 | Domain | IGFBP N-terminal |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000075322 | 10 | 25 | 830_851 | 324.0 | 864.0 | Region | Required for secretion | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000259486 | 11 | 26 | 830_851 | 376.0 | 916.0 | Region | Required for secretion | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000522826 | 10 | 26 | 830_851 | 324.0 | 889.0 | Region | Required for secretion |
- Not-retained protein feature among the 13 regional features. |
Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
Hgene | NOV | chr8:120431585 | chr8:120606100 | ENST00000259526 | + | 4 | 5 | 264_338 | 259.0 | 358.0 | Domain | CTCK |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000075322 | 10 | 25 | 55_98 | 324.0 | 864.0 | Domain | SMB 1 | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000075322 | 10 | 25 | 99_143 | 324.0 | 864.0 | Domain | SMB 2 | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000259486 | 11 | 26 | 55_98 | 376.0 | 916.0 | Domain | SMB 1 | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000259486 | 11 | 26 | 99_143 | 376.0 | 916.0 | Domain | SMB 2 | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000522826 | 10 | 26 | 55_98 | 324.0 | 889.0 | Domain | SMB 1 | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000522826 | 10 | 26 | 99_143 | 324.0 | 889.0 | Domain | SMB 2 | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000075322 | 10 | 25 | 127_129 | 324.0 | 864.0 | Motif | Cell attachment site | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000259486 | 11 | 26 | 127_129 | 376.0 | 916.0 | Motif | Cell attachment site | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000522826 | 10 | 26 | 127_129 | 324.0 | 889.0 | Motif | Cell attachment site | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000075322 | 10 | 25 | 211_214 | 324.0 | 864.0 | Region | Substrate binding | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000075322 | 10 | 25 | 244_255 | 324.0 | 864.0 | Region | Substrate binding | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000259486 | 11 | 26 | 211_214 | 376.0 | 916.0 | Region | Substrate binding | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000259486 | 11 | 26 | 244_255 | 376.0 | 916.0 | Region | Substrate binding | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000522826 | 10 | 26 | 211_214 | 324.0 | 889.0 | Region | Substrate binding | |
Tgene | ENPP2 | chr8:120431585 | chr8:120606100 | ENST00000522826 | 10 | 26 | 244_255 | 324.0 | 889.0 | Region | Substrate binding |
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Fusion Protein Structures |
![]() * Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
Fusion protein PDB link (fusion AA seq ID in FusionPDB) | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | AA seq | Len(AA seq) |
PDB file >>>1689_NOV_120431585_ENPP2_120606100_ranked_0.pdb | NOV | 120431585 | 120431585 | ENST00000427067 | ENPP2 | chr8 | 120606100 | - | MPWQPRGSFHFPTQPISPLLPALQPTGLCASQERAIKPVLGVIGKHRTRGKASSANLQRRKSRLVKARGESLSMQSVQSTSFCLRKQCLC LTFLLLHLLGQVAATQRCPPQCPGRCPATPPTCAPGVRAVLDGCSCCLVCARQRGESCSDLEPCDESSGLYCDRSADPSNQTGICTAVEG DNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGCVPRCQLDVLLPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGV EVSDSSVNCIEQTTEWTACSKSCGMGFSTRVTNRNRQCEMLKQTRLCMVRPCEQEPEQPTDKMTNPLREIDKIVGQLMDGLKQLKLHRCV NVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIE DIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLN HLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEAETRKFRGSRNENKENINGNFEPRKERHL LYGRPAVLYRTRYDILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLS SSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLD | 896 |
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pLDDT score distribution |
![]() * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
ENPP2_pLDDT.png![]() |
![]() * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
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Ramachandran Plot of Fusion Protein Structure |
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Fusion AA seq ID in FusionPDB and their Ramachandran plots |
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Fusion Protein-Protein Interaction |
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Gene | PPI interactors |
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Gene | STRING network |
NOV | |
ENPP2 |
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Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
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Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to NOV-ENPP2 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to NOV-ENPP2 |
![]() (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
Hgene | Tgene | Disease | Source | PMID |
![]() (DisGeNet 4.0) |
Partner | Gene | Disease ID | Disease name | # pubmeds | Source |