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Fusion Protein:NT5C2-GNAI3 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: NT5C2-GNAI3 | FusionPDB ID: 60603 | FusionGDB2.0 ID: 60603 | Hgene | Tgene | Gene symbol | NT5C2 | GNAI3 | Gene ID | 22978 | 2773 |
| Gene name | 5'-nucleotidase, cytosolic II | G protein subunit alpha i3 | |
| Synonyms | GMP|NT5B|PNT5|SPG45|SPG65|cN-II | 87U6|ARCND1 | |
| Cytomap | 10q24.32-q24.33 | 1p13.3 | |
| Type of gene | protein-coding | protein-coding | |
| Description | cytosolic purine 5'-nucleotidase5'-nucleotidase (purine), cytosolic type BIMP-specific 5'-NTepididymis secretory sperm binding proteinspastic paraplegia 45 (autosomal recessive) | guanine nucleotide-binding protein G(i) subunit alphag(i) alpha-3guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3guanine nucleotide-binding protein G(k) subunit alpha | |
| Modification date | 20200313 | 20200322 | |
| UniProtAcc | . | P08754 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000369857, ENST00000343289, ENST00000404739, ENST00000423468, ENST00000470299, | ENST00000369851, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 13 X 8 X 9=936 | 33 X 8 X 14=3696 |
| # samples | 17 | 35 | |
| ** MAII score | log2(17/936*10)=-2.4609737834457 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(35/3696*10)=-3.40053792958373 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: NT5C2 [Title/Abstract] AND GNAI3 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | NT5C2(104899162)-GNAI3(110116358), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a cell metabolism gene due to the frame-shifted ORF. NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF. NT5C2-GNAI3 seems lost the major protein functional domain in Hgene partner, which is a IUPHAR drug target due to the frame-shifted ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Tgene | GNAI3 | GO:0007193 | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway | 19478087 |
| Tgene | GNAI3 | GO:0007212 | dopamine receptor signaling pathway | 19478087 |
| Tgene | GNAI3 | GO:0046039 | GTP metabolic process | 19478087 |
| Tgene | GNAI3 | GO:0051301 | cell division | 17635935 |
| Fusion gene breakpoints across NT5C2 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across GNAI3 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | STAD | TCGA-D7-A6F0 | NT5C2 | chr10 | 104899162 | - | GNAI3 | chr1 | 110116358 | + |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000404739 | NT5C2 | chr10 | 104899162 | - | ENST00000369851 | GNAI3 | chr1 | 110116358 | + | 3177 | 199 | 24 | 1145 | 373 |
| ENST00000423468 | NT5C2 | chr10 | 104899162 | - | ENST00000369851 | GNAI3 | chr1 | 110116358 | + | 3389 | 411 | 275 | 1357 | 360 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000404739 | ENST00000369851 | NT5C2 | chr10 | 104899162 | - | GNAI3 | chr1 | 110116358 | + | 0.000202164 | 0.9997979 |
| ENST00000423468 | ENST00000369851 | NT5C2 | chr10 | 104899162 | - | GNAI3 | chr1 | 110116358 | + | 8.80E-05 | 0.999912 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >60603_60603_1_NT5C2-GNAI3_NT5C2_chr10_104899162_ENST00000404739_GNAI3_chr1_110116358_ENST00000369851_length(amino acids)=373AA_BP=56 MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAGAGESGKSTIVKQMKIIHEDGYSEDECKQYKV VVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDR ISQSNYIPTQQDVLRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFD SICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTD -------------------------------------------------------------- >60603_60603_2_NT5C2-GNAI3_NT5C2_chr10_104899162_ENST00000423468_GNAI3_chr1_110116358_ENST00000369851_length(amino acids)=360AA_BP=43 MFVDPVFSSRGSRSLRMSKEGVFVNRSLAMEKIKCFGFDMDYTLAGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAI IRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTPELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDV LRTRVKTTGIVETHFTFKDLYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSI ILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:104899162/chr1:110116358) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | GNAI3 |
| FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. | FUNCTION: Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935). {ECO:0000269|PubMed:17635935, ECO:0000269|PubMed:18434541, ECO:0000269|PubMed:2535845, ECO:0000269|PubMed:8774883}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 150_151 | 39.333333333333336 | 948.6666666666666 | Nucleotide binding | GTP | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 175_181 | 39.333333333333336 | 948.6666666666666 | Nucleotide binding | GTP | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 200_204 | 39.333333333333336 | 948.6666666666666 | Nucleotide binding | GTP | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 269_272 | 39.333333333333336 | 948.6666666666666 | Nucleotide binding | GTP | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 43_48 | 39.333333333333336 | 948.6666666666666 | Nucleotide binding | GTP | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 173_181 | 39.333333333333336 | 948.6666666666666 | Region | G2 motif | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 196_205 | 39.333333333333336 | 948.6666666666666 | Region | G3 motif | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 265_272 | 39.333333333333336 | 948.6666666666666 | Region | G4 motif | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 324_329 | 39.333333333333336 | 948.6666666666666 | Region | G5 motif |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | NT5C2 | chr10:104899162 | chr1:110116358 | ENST00000343289 | - | 3 | 18 | 549_561 | 58.333333333333336 | 562.0 | Compositional bias | Note=Asp/Glu-rich (acidic) |
| Hgene | NT5C2 | chr10:104899162 | chr1:110116358 | ENST00000404739 | - | 2 | 17 | 549_561 | 58.333333333333336 | 562.0 | Compositional bias | Note=Asp/Glu-rich (acidic) |
| Hgene | NT5C2 | chr10:104899162 | chr1:110116358 | ENST00000343289 | - | 3 | 18 | 202_210 | 58.333333333333336 | 562.0 | Region | Substrate binding |
| Hgene | NT5C2 | chr10:104899162 | chr1:110116358 | ENST00000404739 | - | 2 | 17 | 202_210 | 58.333333333333336 | 562.0 | Region | Substrate binding |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 32_354 | 39.333333333333336 | 948.6666666666666 | Domain | G-alpha | |
| Tgene | GNAI3 | chr10:104899162 | chr1:110116358 | ENST00000369851 | 0 | 9 | 35_48 | 39.333333333333336 | 948.6666666666666 | Region | G1 motif |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| NT5C2 | |
| GNAI3 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to NT5C2-GNAI3 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to NT5C2-GNAI3 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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