UTHEALTH HOME    ABOUT SBMI    A-Z    WEBMAIL    INSIDE THE UNIVERSITY
FusionGDB Logo

Home

Download

Statistics

Examples

Help

Contact

Terms of Use
Center for Computational Systems Medicine level1
leaf

Fusion Gene Summary

leaf

Fusion Gene Sample Information

leaf

Fusion ORF Analysis

leaf

Fusion Amino Acid Sequences

leaf

Fusion Protein Functional Features

leaf

Fusion Protein-Protein Interaction

leaf

Related drugs with this fusion protein

leaf

Related disease with this fusion protein

Fusion Protein:ARHGAP32-AHNAK

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: ARHGAP32-AHNAK
FusionPDB ID: 6067
FusionGDB2.0 ID: 6067
HgeneTgene
Gene symbol

ARHGAP32

AHNAK

Gene ID

9743

79026

Gene nameRho GTPase activating protein 32AHNAK nucleoprotein
SynonymsGC-GAP|GRIT|PX-RICS|RICS|p200RhoGAP|p250GAPAHNAKRS|PM227
Cytomap

11q24.3

11q12.3

Type of geneprotein-codingprotein-coding
Descriptionrho GTPase-activating protein 32GAB-associated CDC42GAB-associated Cdc42/Rac GTPase-activating proteinGTPase regulator interacting with TrkAGTPase-activating protein for Cdc42 and Rac1RhoGAP involved in the -catenin-N-cadherin and NMDA receptor signaneuroblast differentiation-associated protein AHNAKAHNAK-relateddesmoyokin
Modification date2020032720200327
UniProtAcc

A7KAX9

Q8IVF2

Ensembl transtripts involved in fusion geneENST idsENST00000310343, ENST00000524655, 
ENST00000392657, ENST00000527272, 
ENST00000525875, ENST00000378024, 
ENST00000257247, ENST00000530124, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score10 X 9 X 5=45024 X 16 X 9=3456
# samples 1132
** MAII scorelog2(11/450*10)=-2.03242147769238
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(32/3456*10)=-3.43295940727611
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: ARHGAP32 [Title/Abstract] AND AHNAK [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)ARHGAP32(128993341)-AHNAK(62201363), # samples:3
Anticipated loss of major functional domain due to fusion event.ARHGAP32-AHNAK seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ARHGAP32-AHNAK seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
ARHGAP32-AHNAK seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ARHGAP32-AHNAK seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
ARHGAP32-AHNAK seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
ARHGAP32-AHNAK seems lost the major protein functional domain in Tgene partner, which is a tumor suppressor due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID

check buttonFusion gene breakpoints across ARHGAP32 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across AHNAK (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


Top

Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4BRCATCGA-A8-A06Z-01AARHGAP32chr11

128963526

-AHNAKchr11

62249398

-
ChimerDB4BRCATCGA-A8-A06Z-01AARHGAP32chr11

128993341

-AHNAKchr11

62201363

-
ChimerDB4BRCATCGA-A8-A06ZARHGAP32chr11

128993340

-AHNAKchr11

62201363

-


Top

Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000310343ARHGAP32chr11128993341-ENST00000530124AHNAKchr1162201363-7504020455151
ENST00000524655ARHGAP32chr11128993341-ENST00000530124AHNAKchr1162201363-55220455027890
ENST00000310343ARHGAP32chr11128993340-ENST00000530124AHNAKchr1162201363-7504020455151
ENST00000524655ARHGAP32chr11128993340-ENST00000530124AHNAKchr1162201363-55220455027890

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000310343ENST00000530124ARHGAP32chr11128993341-AHNAKchr1162201363-0.0041080380.9958919
ENST00000524655ENST00000530124ARHGAP32chr11128993341-AHNAKchr1162201363-0.4376160.562384
ENST00000310343ENST00000530124ARHGAP32chr11128993340-AHNAKchr1162201363-0.0041080380.9958919
ENST00000524655ENST00000530124ARHGAP32chr11128993340-AHNAKchr1162201363-0.4376160.562384

Top

Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>6067_6067_1_ARHGAP32-AHNAK_ARHGAP32_chr11_128993340_ENST00000310343_AHNAK_chr11_62201363_ENST00000530124_length(amino acids)=151AA_BP=133
METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSVHSEEDDFVPELHRNVHPRERPDWEETLSAMARGADVPEIPGDLTL

--------------------------------------------------------------

>6067_6067_2_ARHGAP32-AHNAK_ARHGAP32_chr11_128993340_ENST00000524655_AHNAK_chr11_62201363_ENST00000530124_length(amino acids)=90AA_BP=
MLDKCLLDKRAKDNHLSTIVAKAPSSPKPKPTLCPLASKEMHQAGLMQQPSVWPGWSYKHACSEKPPRGRYSFNCRCLLPLAQQYVISHF

--------------------------------------------------------------

>6067_6067_3_ARHGAP32-AHNAK_ARHGAP32_chr11_128993341_ENST00000310343_AHNAK_chr11_62201363_ENST00000530124_length(amino acids)=151AA_BP=133
METESESSTLGDDSVFWLESEVIIQVTDCEEEEREEKFRKMKSSVHSEEDDFVPELHRNVHPRERPDWEETLSAMARGADVPEIPGDLTL

--------------------------------------------------------------

>6067_6067_4_ARHGAP32-AHNAK_ARHGAP32_chr11_128993341_ENST00000524655_AHNAK_chr11_62201363_ENST00000530124_length(amino acids)=90AA_BP=
MLDKCLLDKRAKDNHLSTIVAKAPSSPKPKPTLCPLASKEMHQAGLMQQPSVWPGWSYKHACSEKPPRGRYSFNCRCLLPLAQQYVISHF

--------------------------------------------------------------

Top

Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:128993341/chr11:62201363)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
ARHGAP32

A7KAX9

AHNAK

Q8IVF2

FUNCTION: GTPase-activating protein (GAP) promoting GTP hydrolysis on RHOA, CDC42 and RAC1 small GTPases. May be involved in the differentiation of neuronal cells during the formation of neurite extensions. Involved in NMDA receptor activity-dependent actin reorganization in dendritic spines. May mediate cross-talks between Ras- and Rho-regulated signaling pathways in cell growth regulation. Isoform 2 has higher GAP activity (By similarity). {ECO:0000250, ECO:0000269|PubMed:12446789, ECO:0000269|PubMed:12454018, ECO:0000269|PubMed:12531901, ECO:0000269|PubMed:12788081, ECO:0000269|PubMed:12819203, ECO:0000269|PubMed:12857875, ECO:0000269|PubMed:17663722}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247465458_5654147.33333333333334150.0Compositional biasNote=Gly-rich
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024055458_565405891.0Compositional biasNote=Gly-rich
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247465458_5654147.33333333333334150.0Compositional biasNote=Gly-rich
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024055458_565405891.0Compositional biasNote=Gly-rich
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024059_9005891.0DomainPDZ
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024059_9005891.0DomainPDZ
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247464971_4979147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247465019_5027147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247465034_5039147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247465706_5716147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247465772_5779147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024054971_497905891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024055019_502705891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024055034_503905891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024055706_571605891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993340chr11:62201363ENST00000378024055772_577905891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247464971_4979147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247465019_5027147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247465034_5039147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247465706_5716147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247465772_5779147.33333333333334150.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024054971_497905891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024055019_502705891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024055034_503905891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024055706_571605891.0MotifNuclear localization signal
TgeneAHNAKchr11:128993341chr11:62201363ENST00000378024055772_577905891.0MotifNuclear localization signal

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-4221031_1036134.02088.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-4221305_1310134.02088.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-1131031_103601739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-1131305_131001739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-1121031_103601739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-1121305_131001739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-4221031_1036134.02088.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-4221305_1310134.02088.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-1131031_103601739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-1131305_131001739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-1121031_103601739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-1121305_131001739.0Compositional biasNote=Poly-Pro
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-422131_245134.02088.0DomainNote=PX%3B atypical
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-422259_321134.02088.0DomainSH3
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-422372_567134.02088.0DomainRho-GAP
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-113131_24501739.0DomainNote=PX%3B atypical
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-113259_32101739.0DomainSH3
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-113372_56701739.0DomainRho-GAP
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-112131_24501739.0DomainNote=PX%3B atypical
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-112259_32101739.0DomainSH3
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-112372_56701739.0DomainRho-GAP
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-422131_245134.02088.0DomainNote=PX%3B atypical
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-422259_321134.02088.0DomainSH3
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-422372_567134.02088.0DomainRho-GAP
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-113131_24501739.0DomainNote=PX%3B atypical
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-113259_32101739.0DomainSH3
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-113372_56701739.0DomainRho-GAP
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-112131_24501739.0DomainNote=PX%3B atypical
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-112259_32101739.0DomainSH3
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-112372_56701739.0DomainRho-GAP
TgeneAHNAKchr11:128993340chr11:62201363ENST00000257247469_90147.33333333333334150.0DomainPDZ
TgeneAHNAKchr11:128993341chr11:62201363ENST00000257247469_90147.33333333333334150.0DomainPDZ


Top

Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
ARHGAP32SFN, GRIN2B, RHOA, CDC42, GAB2, GAB1, RAC1, CRK, NCK1, BCAR1, FYN, NTRK1, CRKL, SHC2, SHC3, SRC, PLCG1, PIK3R1, GRB2, ARRB1, NPHP1, ATXN1, FZR1, SMURF1, AES, MDFI, NCK2, DZIP3, ABI2, LZTS2, NXF1, EGFR, SHC1, ZWINT, HNRNPD, SORT1, SYNCRIP, SGTB, Cct4, Mad2l1bp, CDH1, PTPRK, ASB3, TRIM25, EGLN3, ESR2, KRAS, KIAA1429, BICD1, LHX2, OBSL1, CUL7, GOLGA2, ARHGAP24, CPEB4, RC3H1, ZFP36, CEP85, KIAA0355, CIT, GPHN, SEC61B, CBS, CHN1, PRDX4, SEC16A, M, NR3C1, ARF6, C11orf52, CAV1, CEP135, CTNNA1, CTNNB1, CXADR, FLOT1, GJA1, KRT19, LCK, LYN, MLLT4, OCLN, PARD3, PRPH, RAB35, RHOB, SQSTM1, STIL, SYNE3, ZYX, VPS33A, YWHAB, CCL3, YWHAG, YWHAQ, YWHAH, TRIM52, VBP1, PFDN1, LOXL4, PFDN2, PFDN4, CLEC11A, CRYBB3, B4GALT2, KLHL14, SULT1C4, HDHD1, CAMK2A, ETV6,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
ARHGAP32all structure
AHNAK


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-4221685_2087134.02088.0FYN
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-1131685_208701739.0FYN
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-1121685_208701739.0FYN
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-4221685_2087134.02088.0FYN
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-1131685_208701739.0FYN
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-1121685_208701739.0FYN
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000310343-4221391_1711134.02088.0GAB2
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000392657-1131391_171101739.0GAB2
HgeneARHGAP32chr11:128993340chr11:62201363ENST00000527272-1121391_171101739.0GAB2
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000310343-4221391_1711134.02088.0GAB2
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000392657-1131391_171101739.0GAB2
HgeneARHGAP32chr11:128993341chr11:62201363ENST00000527272-1121391_171101739.0GAB2


Top

Related Drugs to ARHGAP32-AHNAK


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

Top

Related Diseases to ARHGAP32-AHNAK


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneARHGAP32C0036341Schizophrenia1PSYGENET