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Center for Computational Systems Medicine level3
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Fusion Gene Summary

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Fusion Gene Sample Information

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Fusion ORF Analysis

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Fusion Amino Acid Sequences

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Fusion Protein Functional Features

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Fusion Protein Structure

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pLDDT scores

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Ramachandran Plot of Fusion Protein Structure

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Potential Active Site Information

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Potentially Interacting Small Molecules through Virtual Screening

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Biochemical Features of Small Molecules with ADME

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Drug Toxicity Information

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Fusion Protein-Protein Interaction

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Related drugs with this fusion protein

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Related disease with this fusion protein

Fusion Protein:RET-NCOA4

Fusion Protein Summary

check button Fusion gene summary
Fusion partner gene informationFusion gene name: RET-NCOA4
FusionPDB ID: 73564
FusionGDB2.0 ID: 73564
HgeneTgene
Gene symbol

RET

NCOA4

Gene ID

5979

8031

Gene nameret proto-oncogenenuclear receptor coactivator 4
SynonymsCDHF12|CDHR16|HSCR1|MEN2A|MEN2B|MTC1|PTC|RET-ELE1ARA70|ELE1|PTC3|RFG
Cytomap

10q11.21

10q11.22

Type of geneprotein-codingprotein-coding
Descriptionproto-oncogene tyrosine-protein kinase receptor RetRET receptor tyrosine kinasecadherin family member 12cadherin-related family member 16proto-oncogene c-Retrearranged during transfectionret proto-oncogene (multiple endocrine neoplasia and medullarynuclear receptor coactivator 470 kDa AR-activator70 kDa androgen receptor coactivatorNCoA-4RET-activating gene ELE1androgen receptor-associated protein of 70 kDaret fused
Modification date2020032220200313
UniProtAcc

RTL1

Q13772

Ensembl transtripts involved in fusion geneENST idsENST00000340058, ENST00000355710, 
ENST00000344348, ENST00000374082, 
ENST00000374087, ENST00000414907, 
ENST00000430396, ENST00000443446, 
ENST00000498586, ENST00000438493, 
ENST00000452682, 
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)* DoF score9 X 6 X 4=21610 X 11 X 3=330
# samples 912
** MAII scorelog2(9/216*10)=-1.26303440583379
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0		log2(12/330*10)=-1.4594316186373
possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs).
DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)

PubMed: RET [Title/Abstract] AND NCOA4 [Title/Abstract] AND fusion [Title/Abstract]

Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)NCOA4(51582939)-RET(43612032), # samples:12
RET(43610184)-NCOA4(51584616), # samples:3
Anticipated loss of major functional domain due to fusion event.NCOA4-RET seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
NCOA4-RET seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
RET-NCOA4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
RET-NCOA4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF.
RET-NCOA4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
RET-NCOA4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.
NCOA4-RET seems lost the major protein functional domain in Hgene partner, which is a CGC due to the frame-shifted ORF.
NCOA4-RET seems lost the major protein functional domain in Hgene partner, which is a essential gene due to the frame-shifted ORF.
NCOA4-RET seems lost the major protein functional domain in Hgene partner, which is a tumor suppressor due to the frame-shifted ORF.
NCOA4-RET seems lost the major protein functional domain in Tgene partner, which is a CGC due to the frame-shifted ORF.
NCOA4-RET seems lost the major protein functional domain in Tgene partner, which is a IUPHAR drug target due to the frame-shifted ORF.
NCOA4-RET seems lost the major protein functional domain in Tgene partner, which is a kinase due to the frame-shifted ORF.
* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

check button Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez
PartnerGeneGO IDGO termPubMed ID
HgeneRET

GO:0030155

regulation of cell adhesion

21357690

HgeneRET

GO:0030335

positive regulation of cell migration

20702524

HgeneRET

GO:0033619

membrane protein proteolysis

21357690

HgeneRET

GO:0033630

positive regulation of cell adhesion mediated by integrin

20702524

HgeneRET

GO:0035860

glial cell-derived neurotrophic factor receptor signaling pathway

28953886

HgeneRET

GO:0043410

positive regulation of MAPK cascade

28846099

TgeneNCOA4

GO:0006622

protein targeting to lysosome

25327288


check buttonFusion gene breakpoints across RET (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure

check buttonFusion gene breakpoints across NCOA4 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.
all structure


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Fusion Gene Sample Information

check buttonFusion gene information from FusionGDB2.0.
check button Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.
SourceDiseaseSampleHgeneHchrHbpHstrandTgeneTchrTbpTstrand
ChimerDB4THCATCGA-CE-A482-01ARETchr10

43610184

-NCOA4chr10

51584616

+
ChimerDB4THCATCGA-CE-A482-01ARETchr10

43610184

+NCOA4chr10

51584616

-
ChimerDB4THCATCGA-CE-A482-01ARETchr10

43610184

+NCOA4chr10

51584616

+
ChimerKB3..RETchr10

43610184

+NCOA4chr10

51580879

+
ChimerKB3..RETchr10

43610184

+NCOA4chr10

51582795

+
ChimerKB3..RETchr10

43610184

+NCOA4chr10

51584615

+
ChiTaRS5.0N/AS83049RETchr10

43610087

+NCOA4chr10

51584126

-


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Fusion ORF Analysis


check buttonFusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandSeq length
(transcript)		BP loci
(transcript)		Predicted start
(transcript)		Predicted stop
(transcript)		Seq length
(amino acids)
ENST00000355710RETchr1043610184+ENST00000438493NCOA4chr1051580879+5432236823239301232
ENST00000355710RETchr1043610184+ENST00000452682NCOA4chr1051580879+5521236823239901252
ENST00000340058RETchr1043610184+ENST00000438493NCOA4chr1051580879+5380231618038781232
ENST00000340058RETchr1043610184+ENST00000452682NCOA4chr1051580879+5469231618039381252
ENST00000355710RETchr1043610184+ENST00000438493NCOA4chr1051582795+5144236823236421136
ENST00000355710RETchr1043610184+ENST00000452682NCOA4chr1051582795+5233236823237021156
ENST00000340058RETchr1043610184+ENST00000438493NCOA4chr1051582795+5092231618035901136
ENST00000340058RETchr1043610184+ENST00000452682NCOA4chr1051582795+5181231618036501156
ENST00000355710RETchr1043610184+ENST00000438493NCOA4chr1051584615+5000236823234981088
ENST00000355710RETchr1043610184+ENST00000452682NCOA4chr1051584615+5089236823235581108
ENST00000340058RETchr1043610184+ENST00000438493NCOA4chr1051584615+4948231618034461088
ENST00000340058RETchr1043610184+ENST00000452682NCOA4chr1051584615+5037231618035061108
ENST00000355710RETchr1043610184+ENST00000438493NCOA4chr1051584616-5000236823234981088
ENST00000355710RETchr1043610184+ENST00000452682NCOA4chr1051584616-5089236823235581108
ENST00000340058RETchr1043610184+ENST00000438493NCOA4chr1051584616-4948231618034461088
ENST00000340058RETchr1043610184+ENST00000452682NCOA4chr1051584616-5037231618035061108

check buttonDeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.
HenstTenstHgeneHchrHbpHstrandTgeneTchrTbpTstrandNo-coding scoreCoding score
ENST00000355710ENST00000438493RETchr1043610184+NCOA4chr1051584616-0.0010677460.9989323
ENST00000355710ENST00000452682RETchr1043610184+NCOA4chr1051584616-0.000566260.9994337
ENST00000340058ENST00000438493RETchr1043610184+NCOA4chr1051584616-0.0009854960.9990145
ENST00000340058ENST00000452682RETchr1043610184+NCOA4chr1051584616-0.0005275570.99947244

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Fusion Amino Acid Sequences


check button For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.
>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>73564_73564_1_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51580879_ENST00000438493_length(amino acids)=1232AA_BP=0
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILLLGQFNCL
THQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKS
ASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFS
IEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDH
LEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLC
PRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQL

--------------------------------------------------------------

>73564_73564_2_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51580879_ENST00000452682_length(amino acids)=1252AA_BP=0
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILLLGQFNCL
THQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKS
ASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFS
IEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDH
LEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLC
PRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQL

--------------------------------------------------------------

>73564_73564_3_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51582795_ENST00000438493_length(amino acids)=1136AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILKSASGIVA
VPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKV
GDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKP
LSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVI
EQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDK

--------------------------------------------------------------

>73564_73564_4_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51582795_ENST00000452682_length(amino acids)=1156AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILKSASGIVA
VPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKV
GDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKP
LSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVI
EQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDK

--------------------------------------------------------------

>73564_73564_5_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51584615_ENST00000438493_length(amino acids)=1088AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_6_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51584615_ENST00000452682_length(amino acids)=1108AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_7_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51584616_ENST00000438493_length(amino acids)=1088AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_8_RET-NCOA4_RET_chr10_43610184_ENST00000340058_NCOA4_chr10_51584616_ENST00000452682_length(amino acids)=1108AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_9_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51580879_ENST00000438493_length(amino acids)=1232AA_BP=0
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILLLGQFNCL
THQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKS
ASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFS
IEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDH
LEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLC
PRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQL

--------------------------------------------------------------

>73564_73564_10_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51580879_ENST00000452682_length(amino acids)=1252AA_BP=0
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILLLGQFNCL
THQLECTQNKDLANQVSVCLERLGSLTLKPEDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKRGCISMPEQKS
ASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFS
IEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDH
LEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLC
PRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQL

--------------------------------------------------------------

>73564_73564_11_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51582795_ENST00000438493_length(amino acids)=1136AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILKSASGIVA
VPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKV
GDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKP
LSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVI
EQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDK

--------------------------------------------------------------

>73564_73564_12_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51582795_ENST00000452682_length(amino acids)=1156AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILKSASGIVA
VPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKV
GDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKP
LSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVI
EQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDK

--------------------------------------------------------------

>73564_73564_13_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51584615_ENST00000438493_length(amino acids)=1088AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_14_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51584615_ENST00000452682_length(amino acids)=1108AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_15_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51584616_ENST00000438493_length(amino acids)=1088AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

>73564_73564_16_RET-NCOA4_RET_chr10_43610184_ENST00000355710_NCOA4_chr10_51584616_ENST00000452682_length(amino acids)=1108AA_BP=712
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQE
DTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENR
PPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAP
TFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNR
NLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGR
CEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLC
DELCRTVIAAAVLFSFIVSVLLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSMENQVSVDAFKILTSSRACNF
FNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLFQSYN
VNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTED
RAGKQKFKSPMNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPPDHYGLPAVCDLFACMQLKVDKEKW

--------------------------------------------------------------

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Fusion Protein Functional Features


check button Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr10:51582939/chr10:43612032)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.
FGviewer

check buttonMain function of each fusion partner protein. (from UniProt)
HgeneTgene
RET

RTL1

NCOA4

Q13772

1358FUNCTION: Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. {ECO:0000269|PubMed:10347167}.

check buttonRetention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at

download page

* Minus value of BPloci means that the break pointn is located before the CDS.
- Retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRETchr10:43610184chr10:51584616ENST00000340058+1119168_272712.01073.0DomainCadherin
HgeneRETchr10:43610184chr10:51584616ENST00000355710+1120168_272712.01115.0DomainCadherin
HgeneRETchr10:43610184chr10:51584616ENST00000340058+111929_635712.01073.0Topological domainExtracellular
HgeneRETchr10:43610184chr10:51584616ENST00000355710+112029_635712.01115.0Topological domainExtracellular
HgeneRETchr10:43610184chr10:51584616ENST00000340058+1119636_657712.01073.0TransmembraneHelical
HgeneRETchr10:43610184chr10:51584616ENST00000355710+1120636_657712.01115.0TransmembraneHelical

- Not-retained protein feature among the 13 regional features.
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenProtein featureProtein feature note
HgeneRETchr10:43610184chr10:51584616ENST00000340058+1119724_1016712.01073.0DomainProtein kinase
HgeneRETchr10:43610184chr10:51584616ENST00000355710+1120724_1016712.01115.0DomainProtein kinase
HgeneRETchr10:43610184chr10:51584616ENST00000340058+1119730_738712.01073.0Nucleotide bindingATP
HgeneRETchr10:43610184chr10:51584616ENST00000355710+1120730_738712.01115.0Nucleotide bindingATP
HgeneRETchr10:43610184chr10:51584616ENST00000340058+1119805_807712.01073.0RegionNote=Inhibitors binding
HgeneRETchr10:43610184chr10:51584616ENST00000355710+1120805_807712.01115.0RegionNote=Inhibitors binding
HgeneRETchr10:43610184chr10:51584616ENST00000340058+1119658_1114712.01073.0Topological domainCytoplasmic
HgeneRETchr10:43610184chr10:51584616ENST00000355710+1120658_1114712.01115.0Topological domainCytoplasmic


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Fusion Protein Structures

check button PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.
Fusion protein PDB link (fusion AA seq ID in FusionPDB)HgeneHchrHbpHstrandTgeneTchrTbpTstrandAA seqLen(AA seq)
PDB file (796) >>>796.pdbFusion protein BP residue: 712
CIF file (796) >>>796.cif		RETchr1043610184+NCOA4chr1051584615+
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPL
LYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRS
LDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFF
NTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNI
SVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAR
EEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFD
ADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPV
SLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQL
LVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKG
ITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPR
GIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRTVIAAAVLFSFIVSV
LLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSM
ENQVSVDAFKILTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHS
TTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSE
KFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAK
KPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKA
MTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSP
MNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQE
1088
3D view using mol* of 796 (AA BP:712)
PDB file (804) >>>804.pdbFusion protein BP residue: 712
CIF file (804) >>>804.cif		RETchr1043610184+NCOA4chr1051584615+
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPL
LYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRS
LDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFF
NTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNI
SVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAR
EEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFD
ADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPV
SLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQL
LVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKG
ITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPR
GIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRTVIAAAVLFSFIVSV
LLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSM
ENQVSVDAFKILTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHS
TTSSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSE
KFKLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAK
KPLSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEA
LYKWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKA
MTPSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSP
MNTSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQE
EHNFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQAYFKMNFQDVTVG
1108
3D view using mol* of 804 (AA BP:712)
PDB file (826) >>>826.pdbFusion protein BP residue: 712
CIF file (826) >>>826.cif		RETchr1043610184+NCOA4chr1051582795+
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPL
LYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRS
LDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFF
NTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNI
SVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAR
EEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFD
ADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPV
SLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQL
LVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKG
ITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPR
GIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRTVIAAAVLFSFIVSV
LLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSM
ENQVSVDAFKILKSASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWL
TQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTT
SSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKF
KLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKP
LSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALY
KWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMT
PSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMN
TSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEH
1136
3D view using mol* of 826 (AA BP:712)
PDB file (834) >>>834.pdbFusion protein BP residue: 712
CIF file (834) >>>834.cif		RETchr1043610184+NCOA4chr1051582795+
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPL
LYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRS
LDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFF
NTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNI
SVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAR
EEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFD
ADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPV
SLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQL
LVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKG
ITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPR
GIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRTVIAAAVLFSFIVSV
LLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSM
ENQVSVDAFKILKSASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWL
TQKQTLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTT
SSFSIEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKF
KLLFQSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKP
LSTPSMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALY
KWLLKKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMT
PSRIADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMN
TSWCSFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEH
NFPPDHYGLPAVCDLFACMQLKVDKEKWLYRTPLQAYFKMNFQDVTVGNF
1156
3D view using mol* of 834 (AA BP:712)
PDB file (864) >>>864.pdbFusion protein BP residue: 0
CIF file (864) >>>864.cif		RETchr1043610184+NCOA4chr1051580879+
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPL
LYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRS
LDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFF
NTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNI
SVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAR
EEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFD
ADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPV
SLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQL
LVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKG
ITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPR
GIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRTVIAAAVLFSFIVSV
LLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSM
ENQVSVDAFKILLLGQFNCLTHQLECTQNKDLANQVSVCLERLGSLTLKP
EDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKR
GCISMPEQKSASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQ
TLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFS
IEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLF
QSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTP
SMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLL
KKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRI
ADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWC
SFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPP
1232
3D view using mol* of 864 (AA BP:0)
PDB file (869)CIF file (869) >>>869.cifRETchr1043610184+NCOA4chr1051580879+
MAKATSGAAGLRLLLLLLLPLLGKVALGLYFSRDAYWEKLYVDQAAGTPL
LYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRS
LDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFF
NTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNI
SVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAR
EEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFD
ADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRAT
VHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPV
SLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCS
TLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQL
LVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKG
ITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPR
GIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCRTVIAAAVLFSFIVSV
LLSAFCIHCYHKFAHKPPISSAEMTFRRPAQAFPVSYSSSGARRPSLDSM
ENQVSVDAFKILLLGQFNCLTHQLECTQNKDLANQVSVCLERLGSLTLKP
EDSTVLLFEADTITLRQTITTFGSLKTIQIPEHLMAHASSANIGPFLEKR
GCISMPEQKSASGIVAVPFSEWLLGSKPASGYQAPYIPSTDPQDWLTQKQ
TLENSQTSSRACNFFNNVGGNLKGLENWLLKSEKSSYQKCNSHSTTSSFS
IEMEKVGDQELPDQDEMDLSDWLVTPQESHKLRKPENGSRETSEKFKLLF
QSYNVNDWLVKTDSCTNCQGNQPKGVEIENLGNLKCLNDHLEAKKPLSTP
SMVTEDWLVQNHQDPCKVEEVCRANEPCTSFAECVCDENCEKEALYKWLL
KKEGKDKNGMPVEPKPEPEKHKDSLNMWLCPRKEVIEQTKAPKAMTPSRI
ADSFQVIKNSPLSEWLIRPPYKEGSPKEVPGTEDRAGKQKFKSPMNTSWC
SFNTADWVLPGKKMGNLSQLSSGEDKWLLRKKAQEVLLNSPLQEEHNFPP
DHYGLPAVCDLFACMQLKVDKEKWLYRTPLQAYFKMNFQDVTVGNFQIPC
1252
3D view using mol* of 869 (AA BP:)


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pLDDT score distribution

check button pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
RET_pLDDT.png
all structure
all structure
NCOA4_pLDDT.png
all structure
all structure

check button pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.
RET_NCOA4_796_pLDDT.png (AA BP:712)
all structure
RET_NCOA4_796_pLDDT_and_active_sites.png (AA BP:712)
all structure
RET_NCOA4_796_violinplot.png (AA BP:712)
all structure
RET_NCOA4_804_pLDDT.png (AA BP:712)
all structure
RET_NCOA4_804_pLDDT_and_active_sites.png (AA BP:712)
all structure
RET_NCOA4_804_violinplot.png (AA BP:712)
all structure
RET_NCOA4_826_pLDDT.png (AA BP:712)
all structure
RET_NCOA4_826_pLDDT_and_active_sites.png (AA BP:712)
all structure
RET_NCOA4_826_violinplot.png (AA BP:712)
all structure
RET_NCOA4_834_pLDDT.png (AA BP:712)
all structure
RET_NCOA4_834_pLDDT_and_active_sites.png (AA BP:712)
all structure
RET_NCOA4_834_violinplot.png (AA BP:712)
all structure
RET_NCOA4_864_pLDDT.png (AA BP:0)
all structure
RET_NCOA4_864_pLDDT_and_active_sites.png (AA BP:0)
all structure
RET_NCOA4_864_violinplot.png (AA BP:0)
all structure
RET_NCOA4_869_pLDDT_and_active_sites.png (AA BP:)
all structure
RET_NCOA4_869_violinplot.png (AA BP:)
all structure


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Ramachandran Plot of Fusion Protein Structure


check button Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.
Fusion AA seq ID in FusionPDB and their Ramachandran plots
RET_NCOA4_796.png
all structure
RET_NCOA4_804.png
all structure
RET_NCOA4_826.png
all structure
RET_NCOA4_834.png
all structure
RET_NCOA4_864.png
all structure

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Potential Active Site Information


check button The potential binding sites of these fusion proteins were identified using SiteMap, a module of the Schrodinger suite.
Fusion AA seq ID in FusionPDBSite scoreSizeD scoreVolumeExposureEnclosureContactPhobicPhilicBalanceDon/AccResidues
7961.0732610.949420.5180.4150.8081.0610.4931.4590.3380.764Chain A: 32,33,36,37,38,39,41,50,51,52,54,148,150,
154,160,163,164,166,167,168,169,170,171,172,174,18
5,186,187,188,189,190,191,192,193,241,256,258
8041.07082530.9911428.4070.42760.80391.06290.6741.32270.50960.7687Chain A: 32,33,36,37,38,39,41,50,51,52,54,148,150,
154,160,163,164,167,168,169,170,171,172,174,185,18
6,187,188,189,190,191,192,193,241,256,258
8261.08362480.9696435.9530.4010.82291.08630.61371.42380.4310.6878Chain A: 33,36,37,38,39,41,44,50,51,52,54,148,150,
154,155,160,163,164,166,167,168,169,170,171,172,17
4,185,186,187,188,189,190,191,192,193,241,256,258
8341.09492380.9283363.2370.3850.841.14780.44231.58190.27960.6841Chain A: 32,33,36,37,38,39,41,50,51,52,54,148,150,
154,160,163,164,166,167,168,169,170,171,172,174,18
5,186,187,188,189,190,191,192,193,204,241,256,257,
258
8641.0582710.97419.4890.3940.7851.0610.6331.3530.4680.616Chain A: 32,33,36,37,38,39,41,44,50,51,52,54,148,1
50,154,160,163,164,166,167,168,169,170,171,172,174
,185,186,187,188,189,190,191,192,193,204,241,254,2
56,257,258
8691.1082360.932397.1940.3550.8591.1840.5281.6050.3290.71Chain A: 32,33,36,37,38,39,41,44,50,51,52,54,148,1
50,154,160,163,164,166,167,168,169,170,171,172,174
,185,186,187,188,189,190,191,192,193,204,256,257,2
58

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Potentially Interacting Small Molecules through Virtual Screening


check button The FDA-approved small molecule library molecules were subjected to virtual screening using the Glide.
Fusion AA seq ID in FusionPDBZINC IDDrugBank IDDrug nameDocking scoreGlide gscore
796ZINC000002568036DB01219Dantrolene-6.46879-7.74989
796ZINC000252679615DB01219Dantrolene-6.46879-7.74989
796ZINC000003875368DB00698Nitrofurantoin-6.44203-7.72313
796ZINC000000004319DB00598Labetalol-7.48731-7.51491
796ZINC000000034157DB06707Levonordefrin-7.40451-7.42201
796ZINC000052971887DB06243Eflornithine-6.84476-7.12526
796ZINC000000896546DB01099Flucytosine-7.04448-7.04498
796ZINC000000004321DB00909Zonisamide-7.01525-7.01665
796ZINC000000001735DB00350Minoxidil-6.91019-6.91019
796ZINC000001530600DB00369Cidofovir-6.32425-6.87755
796ZINC000006036847DB00182Amphetamine-6.74-6.7408
796ZINC000003801919DB00630Alendronic acid-6.50568-6.70478
796ZINC000000897258DB00983Formoterol-6.67858-6.69078
796ZINC000100032379DB02631-6.48414-6.65934
796ZINC000000000882DB00173Adenine-6.61164-6.63234
796ZINC000000897256DB00983Formoterol-6.5687-6.5809
796ZINC000000001644DB06795Mafenide-6.54747-6.57707
796ZINC000003812862DB00282Pamidronic acid-6.45839-6.56889
796ZINC000006021033DB00182Amphetamine-6.48317-6.48397
796ZINC000001482184DB00853Temozolomide-6.36783-6.36943

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check button Drug information from DrugBank of the top 20 interacting small molecules.
ZINC IDDrugBank IDDrug nameDrug typeSMILESDrug group
ZINC000000034157DB06707LevonordefrinSmall moleculeC[C@H](N)[C@H](O)C1=CC(O)=C(O)C=C1Approved
ZINC000000896546DB01099FlucytosineSmall moleculeNC1=C(F)C=NC(=O)N1Approved|Investigational
ZINC000000004321DB00909ZonisamideSmall moleculeNS(=O)(=O)CC1=NOC2=CC=CC=C12Approved|Investigational
ZINC000000001735DB00350MinoxidilSmall moleculeNC1=CC(=NC(N)=[N+]1[O-])N1CCCCC1Approved|Investigational
ZINC000001530600DB00369CidofovirSmall moleculeNC1=NC(=O)N(C[C@@H](CO)OCP(O)(O)=O)C=C1Approved
ZINC000006036847DB00182AmphetamineSmall moleculeCC(N)CC1=CC=CC=C1Approved|Illicit|Investigational
ZINC000003801919DB00630Alendronic acidSmall moleculeNCCCC(O)(P(O)(O)=O)P(O)(O)=OApproved
ZINC000000897258DB00983FormoterolSmall moleculeCOC1=CC=C(CC(C)NCC(O)C2=CC(NC=O)=C(O)C=C2)C=C1Approved|Investigational
ZINC000000000882DB00173AdenineSmall moleculeNC1=C2NC=NC2=NC=N1Approved|Nutraceutical
ZINC000000001644DB06795MafenideSmall moleculeNCC1=CC=C(C=C1)S(N)(=O)=OApproved|Vet_approved
ZINC000003812862DB00282Pamidronic acidSmall moleculeNCCC(O)(P(O)(O)=O)P(O)(O)=OApproved
ZINC000001482184DB00853TemozolomideSmall moleculeCN1N=NC2=C(N=CN2C1=O)C(N)=OApproved|Investigational

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Biochemical Features of Small Molecules


check button ADME (Absorption, Distribution, Metabolism, and Excretion) of drugs using QikProp(v3.9)
ZINC IDmol_MWdipoleSASAFOSAFISAPISAWPSAvolumedonorHBaccptHBIPHuman Oral AbsorptionPercent Human Oral AbsorptionRule Of FiveRule Of Three
ZINC000002568036314.2573.867548.75854.323243.185251.250924.46159.205367.05800
ZINC000002568036314.2574.186547.354.729241.127251.4440922.097159.191367.40800
ZINC000252679615314.2573.867548.75854.323243.185251.250924.46159.205367.05800
ZINC000252679615314.2572.557527.99664.383238.233225.380905.494159.686367.50600
ZINC000252679615314.2574.186547.354.729241.127251.4440922.097159.191367.40800
ZINC000252679615314.2572.845525.88465.15235.939224.7950902.055159.671367.87500
ZINC000003875368238.1594.578425.42358.928250.52115.9750683.161159.961253.72200
ZINC000003875368238.1594.877423.94659.354248.434116.1580680.796159.943254.07700
ZINC000000004319328.415.655664.549169.051199.646295.85201140.61545.459.067270.58900
ZINC000000004319328.413.937652.909170.739198.631283.53901134.88245.459.364270.66200
ZINC000000034157183.2071.584399.847106.772186.848106.2270646.19954.28.948252.10900
ZINC000000034157183.2072.284398.836105.841186.106106.8890643.15454.28.781252.1900
ZINC000052971887182.174.815375.577120.033183.002072.541593.19549.616119.75201
ZINC000052971887182.174.577376.305117.956183.727074.623594.335549.723119.67801
ZINC000000896546129.0945.933283.8030174.82165.19643.787412.204349.417259.95700
ZINC000000004321212.2236.978398.51740.32185.554172.6440642.453269.667365.95900
ZINC000000001735209.255.141437.436217.593169.97449.8690711.86443.57.733376.87300
ZINC000001530600279.18910.973485.753106.478301.21375.1182.945819.936512.48.977118.28601
ZINC000001530600279.18913.189481.116107.174307.99561.4654.483817.63512.48.916116.85501
ZINC000006036847135.2081.199369.378132.7747.27189.3390584.758219.281389.94700
ZINC000003801919249.0976.598414.71786.372321.66706.67968037.759.261011
ZINC000003801919249.09713.583415.3388.33320.80706.194681.1837.759.1221011
ZINC000003801919249.0977.425414.52587.312321.45505.758680.61137.759.2441011
ZINC000000897258344.417.083674.489258.736187.926227.82601168.66647.28.855265.22200
ZINC000000897258344.414.982586.764251.201138.136197.42601091.39147.28.677373.27800
ZINC000000000882135.1282.734303.1530170.481132.6720451.892348.538368.3800
ZINC000000000882135.1289.438304.2780171.466132.8120454.287348.639368.21400
ZINC000000897256344.415.264632.493247.284157.494227.71501135.04847.28.825370.83700
ZINC000000897256344.416.478644.939257.318179.642207.97901149.05747.28.565266.25100
ZINC000000001644186.2287.234386.68948.38203.852132.6441.813608.20845.59.936246.46100
ZINC000000001644186.2288.543384.51348.79201.796132.1141.813604.74745.59.902246.800
ZINC000003812862235.079.08382.84949.638327.04906.162620.9837.759.111011
ZINC000003812862235.0711.837382.22754.693321.34806.185620.45137.759.0751011
ZINC000003812862235.074.081379.43356.179318.68404.57620.40637.759.2241011
ZINC000006021033135.2081.199369.378132.7747.27189.3390584.758219.281389.94700
ZINC000001482184194.1523.411379.12887.887235.05156.190597.216279.649251.42500


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Drug Toxicity Information


check button Toxicity information of individual drugs using eToxPred
ZINC IDSmileSurface AccessibilityToxicity
ZINC000002568036O=C1CN(/N=C/c2ccc(-c3ccc([N+](=O)[O-])cc3)o2)C(=O)N10.2040708230.461176527
ZINC000252679615O=C1CN(N=Cc2ccc(-c3ccc([N+](=O)[O-])cc3)o2)C(O)=N10.1491321090.500589886
ZINC000003875368O=C1CN(/N=C/c2ccc([N+](=O)[O-])o2)C(=O)N10.1440232560.52186584
ZINC000000004319C[C@H](CCc1ccccc1)NC[C@H](O)c1ccc(O)c(C(N)=O)c10.1638887930.349082082
ZINC000000034157C[C@H](N)[C@H](O)c1ccc(O)c(O)c10.1350791920.302260037
ZINC000052971887NCCC[C@](N)(C(=O)O)C(F)F0.0864132840.375086254
ZINC000000896546Nc1nc(=O)[nH]cc1F0.1160196950.475808288
ZINC000000004321NS(=O)(=O)Cc1noc2ccccc120.2545692540.46155884
ZINC000000001735Nc1cc(N2CCCCC2)nc(N)[n+]1[O-]0.1297409460.395964399
ZINC000001530600Nc1ccn(C[C@@H](CO)OCP(=O)(O)O)c(=O)n10.0801497210.307248667
ZINC000006036847C[C@@H](N)Cc1ccccc10.4323206060.4471075
ZINC000003801919NCCCC(O)(P(=O)(O)O)P(=O)(O)O0.1119100590.475661392
ZINC000000897258COc1ccc(C[C@@H](C)NC[C@@H](O)c2ccc(O)c(NC=O)c2)cc10.1338630780.246482573
ZINC000100032379N=C1CCCN1Cc1[nH]c(=O)[nH]c(=O)c1Cl0.1131881990.388278234
ZINC000000000882Nc1ncnc2[nH]cnc120.1746372290.465480638
ZINC000000897256COc1ccc(C[C@H](C)NC[C@H](O)c2ccc(O)c(NC=O)c2)cc10.1338630780.246482573
ZINC000000001644NCc1ccc(S(N)(=O)=O)cc10.4977746040.389970963
ZINC000003812862NCCC(O)(P(=O)(O)O)P(=O)(O)O0.0953442180.466020225
ZINC000006021033C[C@H](N)Cc1ccccc10.4323206060.4471075
ZINC000001482184Cn1nnc2c(C(N)=O)ncn2c1=O0.1608864090.261932701


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Fusion Protein-Protein Interaction


check button Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in

ChiPPI page.


check button Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)
GenePPI interactors
NCOA4AR, PPARG, ESR1, Nr3c1, GTF2B, KAT2B, NR3C1, VDR, AKT1, ATXN2, RXRA, HERC2, XPO1, GRIA2, KDR, KIFC3, PSMC1, XRCC3, HUWE1, PSMD14, TFG, KIF21A, USP43, Bmpr1a, Kif1b, Cdc23, Wdr61, Psmd6, FTH1, FTL, GMCL1, NEURL4, TAX1BP1, EGLN3, MAPK6, MID2, ORF3a, nsp13, nsp16, RNF10, PRELID2, PIPSL, BTF3, MAP1LC3B, NBR1, CALCOCO2, OPTN, SQSTM1,
RETSTAT3, DOK6, DOK5, SRC, SHC1, PTPRF, DOK2, DOK4, DOK1, GRB2, CRK, PLCG1, PDLIM7, GRB7, FRS2, NRTN, GFRA1, GRB10, IKBKG, MCRS1, CBL, CBLC, RET, HSP90AA1, NOTCH2NL, NOTCH3, AIP, EGFR, NTRK1, ZBTB48, HNRNPD, SORT1, SYNCRIP, SGTB, BAG6, HIST1H3A, KYNU, TXNL4A, ZCCHC8, ICE2, MAPK3, PTK2, MAPK1, PTPRR, DUSP26, Shc1, PIK3R1, FBXO7, NEDD4, SHANK2, LRRK1, PTK6, CTNNB1, SHC4, ERBB2, ADAM10, SPINT2, PCDH7, LRIG2, APP, GDNF, IGF1R, CLU, EPCAM, CDH2, TGFBR1, PTK7, FAT1, DHCR24, SMN1, PCDH19, NCSTN, CERS2, CHP1, FANCD2, ITM2C, SEL1L, PCDHGB5, CKB, NRP1, PLXNB2, NCK2, CTNND1, TULP3, PTPN1, NF2, CRKL, CAMLG, NUMB, BASP1, ROR2, NOTCH2, PTPN13, ANK3, RAB3GAP1, PEA15, LLGL1, GPRIN3, RAPH1, MARK2, CYFIP1, ARC, GPRIN1, ANKLE2, VRK2, MIB1, PHACTR4, UNC5B, TMEM57, SYAP1, FAF2, ERBB2IP, FAM129B, KIAA1715, PEAK1, VANGL2, BAIAP2, EPN1, NUMBL, PRKAR2A, GAB1, PTPRA, DDX39A, TIMP1, EPHB4, MANEA, SRY, RABAC1, LEMD3, TMEM214, PDZD8, GPR50, SMIM5, RNF149, SDF2L1, BMPR1A, PLEKHH3, ACVR2A,


check button Protein-protein interactors based on sequence similarity (STRING)
GeneSTRING network
RETall structure
NCOA4


check button - Retained interactions in fusion protein (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenStill interaction with


check button - Lost interactions due to fusion (protein functional feature from UniProt).
PartnerGeneHbpTbpENSTStrandBPexonTotalExonProtein feature loci*BPlociTotalLenInteraction lost with


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Related Drugs to RET-NCOA4


check button Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDrugSourcePMID

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Related Diseases to RET-NCOA4


check button Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)
HgeneTgeneDiseaseSourcePMID
RETNCOA4Thyroid Gland Papillary CarcinomaMyCancerGenome
RETNCOA4Adenocarcinoma Of Unknown PrimaryMyCancerGenome
RETNCOA4CholangiocarcinomaMyCancerGenome
RETNCOA4Colon AdenocarcinomaMyCancerGenome
RETNCOA4Colorectal Signet Ring Cell CarcinomaMyCancerGenome

check button Diseases associated with fusion partners.
(DisGeNet 4.0)
PartnerGeneDisease IDDisease name# pubmedsSource
HgeneRETC1833921Familial medullary thyroid carcinoma23CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneRETC3888239HIRSCHSPRUNG DISEASE, SUSCEPTIBILITY TO, 116GENOMICS_ENGLAND;UNIPROT
HgeneRETC0025268Multiple Endocrine Neoplasia Type 2a15CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneRETC1708353Hereditary Paraganglioma-Pheochromocytoma Syndrome12CLINGEN
HgeneRETC0025269Multiple Endocrine Neoplasia Type 2b10CTD_human;GENOMICS_ENGLAND;ORPHANET;UNIPROT
HgeneRETC0238463Papillary thyroid carcinoma3CTD_human;ORPHANET
HgeneRETC1275808Congenital central hypoventilation3CTD_human;GENOMICS_ENGLAND;UNIPROT
HgeneRETC1859049CCHS WITH HIRSCHSPRUNG DISEASE3CTD_human;ORPHANET
HgeneRETC0009402Colorectal Carcinoma2CTD_human;UNIPROT
HgeneRETC0009404Colorectal Neoplasms2CTD_human
HgeneRETC0019569Hirschsprung Disease2CTD_human
HgeneRETC0027662Multiple Endocrine Neoplasia2CTD_human;GENOMICS_ENGLAND
HgeneRETC0085758Aganglionosis, Colonic2CTD_human
HgeneRETC0266294Unilateral agenesis of kidney2ORPHANET
HgeneRETC1257840Aganglionosis, Rectosigmoid Colon2CTD_human
HgeneRETC3661523Congenital Intestinal Aganglionosis2CTD_human
HgeneRETC0006413Burkitt Lymphoma1CTD_human
HgeneRETC0031511Pheochromocytoma1CGI;CTD_human;GENOMICS_ENGLAND;UNIPROT
HgeneRETC0038220Status Epilepticus1CTD_human
HgeneRETC0040136Thyroid Neoplasm1CGI;CTD_human
HgeneRETC0151468Thyroid Gland Follicular Adenoma1CTD_human
HgeneRETC0206693Medullary carcinoma1CTD_human
HgeneRETC0238462Medullary carcinoma of thyroid1CGI;CTD_human
HgeneRETC0270823Petit mal status1CTD_human
HgeneRETC0311335Grand Mal Status Epilepticus1CTD_human
HgeneRETC0343640African Burkitt's lymphoma1CTD_human
HgeneRETC0393734Complex Partial Status Epilepticus1CTD_human
HgeneRETC0549473Thyroid carcinoma1CGI;CTD_human;UNIPROT
HgeneRETC0740340Amyloidosis, Familial1CTD_human
HgeneRETC0751522Status Epilepticus, Subclinical1CTD_human
HgeneRETC0751523Non-Convulsive Status Epilepticus1CTD_human
HgeneRETC0751524Simple Partial Status Epilepticus1CTD_human
HgeneRETC1257877Pheochromocytoma, Extra-Adrenal1CTD_human
HgeneRETC1609433Congenital absence of kidneys syndrome1CTD_human;GENOMICS_ENGLAND;ORPHANET
HgeneRETC3501843Nonmedullary Thyroid Carcinoma1CTD_human
HgeneRETC3501844Familial Nonmedullary Thyroid Cancer1CTD_human
HgeneRETC4721444Burkitt Leukemia1CTD_human
TgeneNCOA4C0238463Papillary thyroid carcinoma2ORPHANET
TgeneNCOA4C0040136Thyroid Neoplasm1CTD_human
TgeneNCOA4C0151468Thyroid Gland Follicular Adenoma1CTD_human
TgeneNCOA4C0549473Thyroid carcinoma1CTD_human