UTHEALTH HOME ABOUT SBMI A-Z WEBMAIL INSIDE THE UNIVERSITY

Home

Download

Statistics

Examples

Help

Contact

level1

	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:ATG16L2-TP53BP2

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: ATG16L2-TP53BP2
	FusionPDB ID: 7539
	FusionGDB2.0 ID: 7539
		Hgene	Tgene
	Gene symbol	ATG16L2	TP53BP2
	Gene ID	89849	7159
	Gene name	autophagy related 16 like 2	tumor protein p53 binding protein 2
	Synonyms	ATG16B\|WDR80	53BP2\|ASPP2\|BBP\|P53BP2\|PPP1R13A
	Cytomap	11q13.4	1q41
	Type of gene	protein-coding	protein-coding
	Description	autophagy-related protein 16-2APG16-like 2ATG16 autophagy related 16-like 2WD repeat-containing protein 80	apoptosis-stimulating of p53 protein 2BCL2-binding proteinapoptosis-stimulating protein of p53, 2renal carcinoma antigen NY-REN-51tumor suppressor p53-binding protein 2
	Modification date	20200313	20200313
	UniProtAcc	Q8NAA4	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000321297, ENST00000534905, ENST00000451353,	ENST00000498843, ENST00000343537, ENST00000391878, ENST00000391879,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	2 X 2 X 2=8	3 X 3 X 3=27
	# samples	2	3
	** MAII score	log2(2/8*10)=1.32192809488736	log2(3/27*10)=0.15200309344505 effective Gene in Pan-Cancer Fusion Genes (eGinPCFGs). DoF>8 and MAII>0
Context (manual curation of fusion genes in FusionPDB)	PubMed: ATG16L2 [Title/Abstract] AND TP53BP2 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	ATG16L2(72528900)-TP53BP2(223980224), # samples:1
Anticipated loss of major functional domain due to fusion event.	ATG16L2-TP53BP2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ATG16L2-TP53BP2 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. ATG16L2-TP53BP2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. ATG16L2-TP53BP2 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Tgene	TP53BP2	GO:0072332	intrinsic apoptotic signaling pathway by p53 class mediator	11684014

Fusion gene breakpoints across ATG16L2 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across TP53BP2 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Top

Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	OV	TCGA-24-1434	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-

Top

Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000321297	ATG16L2	chr11	72528900	+	ENST00000391878	TP53BP2	chr1	223980224	-	1953	456	138	998	286
ENST00000321297	ATG16L2	chr11	72528900	+	ENST00000343537	TP53BP2	chr1	223980224	-	1953	456	138	998	286
ENST00000321297	ATG16L2	chr11	72528900	+	ENST00000391879	TP53BP2	chr1	223980224	-	1207	456	138	998	286
ENST00000534905	ATG16L2	chr11	72528900	+	ENST00000391878	TP53BP2	chr1	223980224	-	1855	358	40	900	286
ENST00000534905	ATG16L2	chr11	72528900	+	ENST00000343537	TP53BP2	chr1	223980224	-	1855	358	40	900	286
ENST00000534905	ATG16L2	chr11	72528900	+	ENST00000391879	TP53BP2	chr1	223980224	-	1109	358	40	900	286

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000321297	ENST00000391878	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-	0.000522517	0.99947757
ENST00000321297	ENST00000343537	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-	0.000522517	0.99947757
ENST00000321297	ENST00000391879	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-	0.001789875	0.9982102
ENST00000534905	ENST00000391878	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-	0.000452603	0.9995474
ENST00000534905	ENST00000343537	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-	0.000452603	0.9995474
ENST00000534905	ENST00000391879	ATG16L2	chr11	72528900	+	TP53BP2	chr1	223980224	-	0.001180797	0.9988192

Top

Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>7539_7539_1_ATG16L2-TP53BP2_ATG16L2_chr11_72528900_ENST00000321297_TP53BP2_chr1_223980224_ENST00000343537_length(amino acids)=286AA_BP=106
MAGPGVPGAPAARWKRHIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALR
VKWQEEEEGLRLVCGEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMT
YSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRN

--------------------------------------------------------------

>7539_7539_2_ATG16L2-TP53BP2_ATG16L2_chr11_72528900_ENST00000321297_TP53BP2_chr1_223980224_ENST00000391878_length(amino acids)=286AA_BP=106
MAGPGVPGAPAARWKRHIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALR
VKWQEEEEGLRLVCGEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMT
YSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRN

--------------------------------------------------------------

>7539_7539_3_ATG16L2-TP53BP2_ATG16L2_chr11_72528900_ENST00000321297_TP53BP2_chr1_223980224_ENST00000391879_length(amino acids)=286AA_BP=106
MAGPGVPGAPAARWKRHIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALR
VKWQEEEEGLRLVCGEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMT
YSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRN

--------------------------------------------------------------

>7539_7539_4_ATG16L2-TP53BP2_ATG16L2_chr11_72528900_ENST00000534905_TP53BP2_chr1_223980224_ENST00000343537_length(amino acids)=286AA_BP=106
MAGPGVPGAPAARWKRHIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALR
VKWQEEEEGLRLVCGEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMT
YSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRN

--------------------------------------------------------------

>7539_7539_5_ATG16L2-TP53BP2_ATG16L2_chr11_72528900_ENST00000534905_TP53BP2_chr1_223980224_ENST00000391878_length(amino acids)=286AA_BP=106
MAGPGVPGAPAARWKRHIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALR
VKWQEEEEGLRLVCGEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMT
YSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRN

--------------------------------------------------------------

>7539_7539_6_ATG16L2-TP53BP2_ATG16L2_chr11_72528900_ENST00000534905_TP53BP2_chr1_223980224_ENST00000391879_length(amino acids)=286AA_BP=106
MAGPGVPGAPAARWKRHIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALR
VKWQEEEEGLRLVCGEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMT
YSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRN

--------------------------------------------------------------

Top

Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr11:72528900/chr1:223980224)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
ATG16L2 Q8NAA4	.
FUNCTION: May play a role in regulating epithelial homeostasis in an ATG16L1-dependent manner. {ECO:0000250\|UniProtKB:Q6KAU8}.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

824_902

825.0

1006.0

Compositional bias

Note=Pro-rich

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

1057_1119

954.0

1135.0

Domain

SH3

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

1057_1119

825.0

1006.0

Domain

SH3

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

866_875

825.0

1006.0

Motif

SH3-binding

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

1025_1067

954.0

1135.0

Repeat

Note=ANK 4

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

958_990

954.0

1135.0

Repeat

Note=ANK 2

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

991_1024

954.0

1135.0

Repeat

Note=ANK 3

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

1025_1067

825.0

1006.0

Repeat

Note=ANK 4

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

926_957

825.0

1006.0

Repeat

Note=ANK 1

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

958_990

825.0

1006.0

Repeat

Note=ANK 2

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

991_1024

825.0

1006.0

Repeat

Note=ANK 3

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

116_227

106.0

620.0

Coiled coil

Ontology_term=ECO:0000255

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

334_373

106.0

620.0

Repeat

WD 1

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

378_417

106.0

620.0

Repeat

WD 2

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

420_454

106.0

620.0

Repeat

WD 3

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

455_498

106.0

620.0

Repeat

WD 4

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

500_539

106.0

620.0

Repeat

WD 5

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

546_585

106.0

620.0

Repeat

WD 6

Hgene

ATG16L2

chr11:72528900

chr1:223980224

ENST00000321297

589_619

106.0

620.0

Repeat

WD 7

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

132_173

954.0

1135.0

Compositional bias

Note=Gln-rich

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

824_902

954.0

1135.0

Compositional bias

Note=Pro-rich

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000391878

132_173

825.0

1006.0

Compositional bias

Note=Gln-rich

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

866_875

954.0

1135.0

Motif

SH3-binding

Tgene

TP53BP2

chr11:72528900

chr1:223980224

ENST00000343537

926_957

954.0

1135.0

Repeat

Note=ANK 1

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
ATG16L2
TP53BP2

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner	Gene	Hbp	Tbp	ENST	Strand	BPexon	TotalExon	Protein feature loci	*BPloci	TotalLen	Interaction lost with
Tgene	TP53BP2	chr11:72528900	chr1:223980224	ENST00000343537		13	18	332_348	954.0	1135.0	APPBP1
Tgene	TP53BP2	chr11:72528900	chr1:223980224	ENST00000391878		14	19	332_348	825.0	1006.0	APPBP1

Top

Related Drugs to ATG16L2-TP53BP2

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to ATG16L2-TP53BP2

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source