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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:SAE1-ZBTB45

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SAE1-ZBTB45
	FusionPDB ID: 79021
	FusionGDB2.0 ID: 79021
		Hgene	Tgene
	Gene symbol	SAE1	ZBTB45
	Gene ID	10055	84878
	Gene name	SUMO1 activating enzyme subunit 1	zinc finger and BTB domain containing 45
	Synonyms	AOS1\|HSPC140\|SUA1\|UBLE1A	ZNF499
	Cytomap	19q13.32	19q13.43
	Type of gene	protein-coding	protein-coding
	Description	SUMO-activating enzyme subunit 1SUMO-1 activating enzyme E1 N subunitSUMO-1 activating enzyme subunit 1activator of SUMO1sentrin/SUMO-activating protein AOS1ubiquitin-like 1-activating enzyme E1Aubiquitin-like protein SUMO-1 activating enzyme	zinc finger and BTB domain-containing protein 45zinc finger protein 499
	Modification date	20200313	20200313
	UniProtAcc	.	.
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000540850, ENST00000270225, ENST00000392776, ENST00000413379, ENST00000598840,	ENST00000354590, ENST00000594051, ENST00000600990,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	17 X 10 X 12=2040	5 X 4 X 5=100
	# samples	19	7
	** MAII score	log2(19/2040*10)=-3.42449782852791 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0	log2(7/100*10)=-0.514573172829758 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: SAE1 [Title/Abstract] AND ZBTB45 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SAE1(47646862)-ZBTB45(59029040), # samples:4
Anticipated loss of major functional domain due to fusion event.	SAE1-ZBTB45 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SAE1-ZBTB45 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Hgene	SAE1	GO:0016925	protein sumoylation	15660128\|20164921
Hgene	SAE1	GO:0033235	positive regulation of protein sumoylation	10187858

Fusion gene breakpoints across SAE1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across ZBTB45 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BRCA	TCGA-C8-A26Z-01A	SAE1	chr19	47646862	-	ZBTB45	chr19	59029040	-
ChimerDB4	BRCA	TCGA-C8-A26Z-01A	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-
ChimerDB4	BRCA	TCGA-C8-A26Z	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000413379	SAE1	chr19	47646862	+	ENST00000354590	ZBTB45	chr19	59029040	-	2372	312	18	1847	609
ENST00000413379	SAE1	chr19	47646862	+	ENST00000594051	ZBTB45	chr19	59029040	-	2372	312	18	1847	609
ENST00000413379	SAE1	chr19	47646862	+	ENST00000600990	ZBTB45	chr19	59029040	-	2336	312	18	1847	609
ENST00000598840	SAE1	chr19	47646862	+	ENST00000354590	ZBTB45	chr19	59029040	-	2363	303	9	1838	609
ENST00000598840	SAE1	chr19	47646862	+	ENST00000594051	ZBTB45	chr19	59029040	-	2363	303	9	1838	609
ENST00000598840	SAE1	chr19	47646862	+	ENST00000600990	ZBTB45	chr19	59029040	-	2327	303	9	1838	609
ENST00000270225	SAE1	chr19	47646862	+	ENST00000354590	ZBTB45	chr19	59029040	-	2338	278	29	1813	594
ENST00000270225	SAE1	chr19	47646862	+	ENST00000594051	ZBTB45	chr19	59029040	-	2338	278	29	1813	594
ENST00000270225	SAE1	chr19	47646862	+	ENST00000600990	ZBTB45	chr19	59029040	-	2302	278	29	1813	594
ENST00000392776	SAE1	chr19	47646862	+	ENST00000354590	ZBTB45	chr19	59029040	-	2306	246	36	1781	581
ENST00000392776	SAE1	chr19	47646862	+	ENST00000594051	ZBTB45	chr19	59029040	-	2306	246	36	1781	581
ENST00000392776	SAE1	chr19	47646862	+	ENST00000600990	ZBTB45	chr19	59029040	-	2270	246	36	1781	581

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000413379	ENST00000354590	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.04901894	0.9509811
ENST00000413379	ENST00000594051	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.04901894	0.9509811
ENST00000413379	ENST00000600990	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.052273043	0.94772696
ENST00000598840	ENST00000354590	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.047846153	0.95215386
ENST00000598840	ENST00000594051	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.047846153	0.95215386
ENST00000598840	ENST00000600990	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.050952002	0.94904804
ENST00000270225	ENST00000354590	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.049236782	0.9507633
ENST00000270225	ENST00000594051	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.049236782	0.9507633
ENST00000270225	ENST00000600990	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.05261403	0.94738597
ENST00000392776	ENST00000354590	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.05377739	0.9462226
ENST00000392776	ENST00000594051	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.05377739	0.9462226
ENST00000392776	ENST00000600990	SAE1	chr19	47646862	+	ZBTB45	chr19	59029040	-	0.058022533	0.9419775

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>79021_79021_1_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000270225_ZBTB45_chr19_59029040_ENST00000354590_length(amino acids)=594AA_BP=82
MSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQMAAAEAV
HHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQG
EALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTP
AKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAG
SAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPE
AAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVR

--------------------------------------------------------------

>79021_79021_2_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000270225_ZBTB45_chr19_59029040_ENST00000594051_length(amino acids)=594AA_BP=82
MSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQMAAAEAV
HHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQG
EALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTP
AKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAG
SAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPE
AAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVR

--------------------------------------------------------------

>79021_79021_3_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000270225_ZBTB45_chr19_59029040_ENST00000600990_length(amino acids)=594AA_BP=82
MSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQMAAAEAV
HHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQG
EALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTP
AKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAG
SAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPE
AAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVR

--------------------------------------------------------------

>79021_79021_4_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000392776_ZBTB45_chr19_59029040_ENST00000354590_length(amino acids)=581AA_BP=69
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQMAAAEAVHHIHLQNFSRSLL
ETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQGEALQVLTAASVLR
IQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTPAKAEGPDADPSLS
AAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAGSAEDVFPDSYVST
WHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPEAAPSTQLGEVPAP
SAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVRAFQCAVCAKRFTQ

--------------------------------------------------------------

>79021_79021_5_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000392776_ZBTB45_chr19_59029040_ENST00000594051_length(amino acids)=581AA_BP=69
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQMAAAEAVHHIHLQNFSRSLL
ETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQGEALQVLTAASVLR
IQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTPAKAEGPDADPSLS
AAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAGSAEDVFPDSYVST
WHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPEAAPSTQLGEVPAP
SAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVRAFQCAVCAKRFTQ

--------------------------------------------------------------

>79021_79021_6_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000392776_ZBTB45_chr19_59029040_ENST00000600990_length(amino acids)=581AA_BP=69
MVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKGLTMLDHEQMAAAEAVHHIHLQNFSRSLL
ETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQGEALQVLTAASVLR
IQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTPAKAEGPDADPSLS
AAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAGSAEDVFPDSYVST
WHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPEAAPSTQLGEVPAP
SAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVRAFQCAVCAKRFTQ

--------------------------------------------------------------

>79021_79021_7_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000413379_ZBTB45_chr19_59029040_ENST00000354590_length(amino acids)=609AA_BP=97
MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG
LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ
LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK
QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL
ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP
APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL

--------------------------------------------------------------

>79021_79021_8_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000413379_ZBTB45_chr19_59029040_ENST00000594051_length(amino acids)=609AA_BP=97
MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG
LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ
LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK
QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL
ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP
APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL

--------------------------------------------------------------

>79021_79021_9_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000413379_ZBTB45_chr19_59029040_ENST00000600990_length(amino acids)=609AA_BP=97
MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG
LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ
LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK
QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL
ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP
APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL

--------------------------------------------------------------

>79021_79021_10_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000598840_ZBTB45_chr19_59029040_ENST00000354590_length(amino acids)=609AA_BP=97
MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG
LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ
LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK
QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL
ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP
APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL

--------------------------------------------------------------

>79021_79021_11_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000598840_ZBTB45_chr19_59029040_ENST00000594051_length(amino acids)=609AA_BP=97
MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG
LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ
LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK
QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL
ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP
APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL

--------------------------------------------------------------

>79021_79021_12_SAE1-ZBTB45_SAE1_chr19_47646862_ENST00000598840_ZBTB45_chr19_59029040_ENST00000600990_length(amino acids)=609AA_BP=97
MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG
LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ
LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK
QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL
ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP
APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL

--------------------------------------------------------------

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr19:47646862/chr19:59029040)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	.
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

128_197

0.0

512.0

Compositional bias

Note=Pro-rich

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

299_401

0.0

512.0

Compositional bias

Note=Pro-rich

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

128_197

0.0

512.0

Compositional bias

Note=Pro-rich

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

299_401

0.0

512.0

Compositional bias

Note=Pro-rich

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

128_197

0.0

512.0

Compositional bias

Note=Pro-rich

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

299_401

0.0

512.0

Compositional bias

Note=Pro-rich

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

33_96

0.0

512.0

Domain

BTB

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

33_96

0.0

512.0

Domain

BTB

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

33_96

0.0

512.0

Domain

BTB

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

403_425

0.0

512.0

Zinc finger

C2H2-type 1

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

431_453

0.0

512.0

Zinc finger

C2H2-type 2

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

459_481

0.0

512.0

Zinc finger

C2H2-type 3

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000354590

486_508

0.0

512.0

Zinc finger

C2H2-type 4

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

403_425

0.0

512.0

Zinc finger

C2H2-type 1

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

431_453

0.0

512.0

Zinc finger

C2H2-type 2

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

459_481

0.0

512.0

Zinc finger

C2H2-type 3

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000594051

486_508

0.0

512.0

Zinc finger

C2H2-type 4

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

403_425

0.0

512.0

Zinc finger

C2H2-type 1

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

431_453

0.0

512.0

Zinc finger

C2H2-type 2

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

459_481

0.0

512.0

Zinc finger

C2H2-type 3

Tgene

ZBTB45

chr19:47646862

chr19:59029040

ENST00000600990

486_508

0.0

512.0

Zinc finger

C2H2-type 4

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Top

Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1287_SAE1_47646862_ZBTB45_59029040_1287_SAE1_47646862_ZBTB45_59029040_ranked_0.pdb	SAE1	47646862	47646862	ENST00000600990	ZBTB45	chr19	59029040	-	MPAAVGGARVRRAVGLSGTGAEAGRAGAMVEKEEAGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLLVGLKGLGAEIAKNLILAGVKG LTMLDHEQMAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQ LVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRK QRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGL ADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGP APAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSL	609

Top

pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

SAE1_pLDDT.png

ZBTB45_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

Top

Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

Top

Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
SAE1
ZBTB45

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

Top

Related Drugs to SAE1-ZBTB45

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to SAE1-ZBTB45

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source