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	Fusion Gene Summary
	Fusion Gene Sample Information
	Fusion ORF Analysis
	Fusion Amino Acid Sequences
	Fusion Protein Functional Features
	Fusion Protein Structure
	pLDDT scores
	Ramachandran Plot of Fusion Protein Structure
	Fusion Protein-Protein Interaction
	Related drugs with this fusion protein
	Related disease with this fusion protein

Fusion Protein:SWT1-DCAF8

Fusion Protein Summary

Fusion gene summary

Fusion partner gene information	Fusion gene name: SWT1-DCAF8
	FusionPDB ID: 88320
	FusionGDB2.0 ID: 88320
		Hgene	Tgene
	Gene symbol	SWT1	DCAF8
	Gene ID	54823	50717
	Gene name	SWT1 RNA endoribonuclease homolog	DDB1 and CUL4 associated factor 8
	Synonyms	C1orf26\|HsSwt1	GAN2\|H326\|WDR42A
	Cytomap	1q25.3	1q23.2
	Type of gene	protein-coding	protein-coding
	Description	transcriptional protein SWT1	DDB1- and CUL4-associated factor 8WD repeat domain 42AWD repeat-containing protein 42A
	Modification date	20200313	20200313
	UniProtAcc	.	Q5TAQ9
Ensembl transtripts involved in fusion gene	ENST ids	ENST00000367500, ENST00000367501,	ENST00000475733, ENST00000610139, ENST00000326837, ENST00000368073, ENST00000368074, ENST00000556710, ENST00000608310,
Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0)	* DoF score	2 X 2 X 2=8	9 X 11 X 5=495
	# samples	2	11
	** MAII score	log2(2/8*10)=1.32192809488736	log2(11/495*10)=-2.16992500144231 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0
Context (manual curation of fusion genes in FusionPDB)	PubMed: SWT1 [Title/Abstract] AND DCAF8 [Title/Abstract] AND fusion [Title/Abstract]
Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0)	SWT1(185159774)-DCAF8(160195453), # samples:4
Anticipated loss of major functional domain due to fusion event.	SWT1-DCAF8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SWT1-DCAF8 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. SWT1-DCAF8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. SWT1-DCAF8 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF.

* DoF score (Degree of Frequency) = # partners X # break points X # cancer types
** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10)

Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner

Gene

GO ID

GO term

PubMed ID

Fusion gene breakpoints across SWT1 (5'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Fusion gene breakpoints across DCAF8 (3'-gene)
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

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Fusion Gene Sample Information

Fusion gene information from FusionGDB2.0.

Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0)
* All genome coordinats were lifted-over on hg19.
* Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser.

Source	Disease	Sample	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand
ChimerDB4	BLCA	TCGA-4Z-AA7R-01A	SWT1	chr1	185159774	-	DCAF8	chr1	160195453	-
ChimerDB4	BLCA	TCGA-4Z-AA7R-01A	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-
ChimerDB4	BLCA	TCGA-4Z-AA7R	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-

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Fusion ORF Analysis

Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	Seq length (transcript)	BP loci (transcript)	Predicted start (transcript)	Predicted stop (transcript)	Seq length (amino acids)
ENST00000367501	SWT1	chr1	185159774	+	ENST00000368073	DCAF8	chr1	160195453	-	4218	1617	94	2340	748
ENST00000367501	SWT1	chr1	185159774	+	ENST00000326837	DCAF8	chr1	160195453	-	4218	1617	94	2340	748
ENST00000367501	SWT1	chr1	185159774	+	ENST00000368074	DCAF8	chr1	160195453	-	4218	1617	94	2340	748
ENST00000367501	SWT1	chr1	185159774	+	ENST00000608310	DCAF8	chr1	160195453	-	2469	1617	94	2340	748
ENST00000367501	SWT1	chr1	185159774	+	ENST00000556710	DCAF8	chr1	160195453	-	2469	1617	94	2340	748
ENST00000367500	SWT1	chr1	185159774	+	ENST00000368073	DCAF8	chr1	160195453	-	4289	1688	87	2411	774
ENST00000367500	SWT1	chr1	185159774	+	ENST00000326837	DCAF8	chr1	160195453	-	4289	1688	87	2411	774
ENST00000367500	SWT1	chr1	185159774	+	ENST00000368074	DCAF8	chr1	160195453	-	4289	1688	87	2411	774
ENST00000367500	SWT1	chr1	185159774	+	ENST00000608310	DCAF8	chr1	160195453	-	2540	1688	87	2411	774
ENST00000367500	SWT1	chr1	185159774	+	ENST00000556710	DCAF8	chr1	160195453	-	2540	1688	87	2411	774

DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated.

Henst	Tenst	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	No-coding score	Coding score
ENST00000367501	ENST00000368073	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000352274	0.99964774
ENST00000367501	ENST00000326837	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000352274	0.99964774
ENST00000367501	ENST00000368074	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000352274	0.99964774
ENST00000367501	ENST00000608310	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.001216564	0.9987834
ENST00000367501	ENST00000556710	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.001216564	0.9987834
ENST00000367500	ENST00000368073	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000350364	0.99964964
ENST00000367500	ENST00000326837	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000350364	0.99964964
ENST00000367500	ENST00000368074	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000350364	0.99964964
ENST00000367500	ENST00000608310	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000998219	0.9990018
ENST00000367500	ENST00000556710	SWT1	chr1	185159774	+	DCAF8	chr1	160195453	-	0.000998219	0.9990018

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Fusion Amino Acid Sequences

For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones.

>FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP

>88320_88320_1_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367500_DCAF8_chr1_160195453_ENST00000326837_length(amino acids)=774AA_BP=532
MGGGGPSPLAWGSGVATAAGAGKLFRMSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLY
YNIKRRQGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSP
KIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTL
QKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSE
SIQDADQEMQIVEELHAARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPW
VVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRK
IDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSG
SDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSH

--------------------------------------------------------------

>88320_88320_2_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367500_DCAF8_chr1_160195453_ENST00000368073_length(amino acids)=774AA_BP=532
MGGGGPSPLAWGSGVATAAGAGKLFRMSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLY
YNIKRRQGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSP
KIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTL
QKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSE
SIQDADQEMQIVEELHAARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPW
VVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRK
IDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSG
SDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSH

--------------------------------------------------------------

>88320_88320_3_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367500_DCAF8_chr1_160195453_ENST00000368074_length(amino acids)=774AA_BP=532
MGGGGPSPLAWGSGVATAAGAGKLFRMSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLY
YNIKRRQGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSP
KIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTL
QKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSE
SIQDADQEMQIVEELHAARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPW
VVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRK
IDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSG
SDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSH

--------------------------------------------------------------

>88320_88320_4_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367500_DCAF8_chr1_160195453_ENST00000556710_length(amino acids)=774AA_BP=532
MGGGGPSPLAWGSGVATAAGAGKLFRMSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLY
YNIKRRQGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSP
KIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTL
QKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSE
SIQDADQEMQIVEELHAARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPW
VVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRK
IDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSG
SDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSH

--------------------------------------------------------------

>88320_88320_5_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367500_DCAF8_chr1_160195453_ENST00000608310_length(amino acids)=774AA_BP=532
MGGGGPSPLAWGSGVATAAGAGKLFRMSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLY
YNIKRRQGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSP
KIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTL
QKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSE
SIQDADQEMQIVEELHAARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPW
VVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRK
IDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSG
SDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSH

--------------------------------------------------------------

>88320_88320_6_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367501_DCAF8_chr1_160195453_ENST00000326837_length(amino acids)=748AA_BP=506
MSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLYYNIKRRQGLKRLSVEIDTLRRRPKIG
SSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSPKIASDVKPKAEGQASENKWSHLLVQR
EKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTLQKLVEENVFNIDSNNSKTKQEEREYL
ESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSESIQDADQEMQIVEELHAARVGKSVDL
PGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPWVVMQELDRMKEGKLLKRAQHKAIPAV
HFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRKIDENENNGVLKKFCPHHLVNSESKAN
ITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGG
VVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGAT

--------------------------------------------------------------

>88320_88320_7_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367501_DCAF8_chr1_160195453_ENST00000368073_length(amino acids)=748AA_BP=506
MSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLYYNIKRRQGLKRLSVEIDTLRRRPKIG
SSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSPKIASDVKPKAEGQASENKWSHLLVQR
EKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTLQKLVEENVFNIDSNNSKTKQEEREYL
ESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSESIQDADQEMQIVEELHAARVGKSVDL
PGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPWVVMQELDRMKEGKLLKRAQHKAIPAV
HFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRKIDENENNGVLKKFCPHHLVNSESKAN
ITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGG
VVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGAT

--------------------------------------------------------------

>88320_88320_8_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367501_DCAF8_chr1_160195453_ENST00000368074_length(amino acids)=748AA_BP=506
MSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLYYNIKRRQGLKRLSVEIDTLRRRPKIG
SSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSPKIASDVKPKAEGQASENKWSHLLVQR
EKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTLQKLVEENVFNIDSNNSKTKQEEREYL
ESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSESIQDADQEMQIVEELHAARVGKSVDL
PGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPWVVMQELDRMKEGKLLKRAQHKAIPAV
HFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRKIDENENNGVLKKFCPHHLVNSESKAN
ITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGG
VVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGAT

--------------------------------------------------------------

>88320_88320_9_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367501_DCAF8_chr1_160195453_ENST00000556710_length(amino acids)=748AA_BP=506
MSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLYYNIKRRQGLKRLSVEIDTLRRRPKIG
SSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSPKIASDVKPKAEGQASENKWSHLLVQR
EKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTLQKLVEENVFNIDSNNSKTKQEEREYL
ESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSESIQDADQEMQIVEELHAARVGKSVDL
PGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPWVVMQELDRMKEGKLLKRAQHKAIPAV
HFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRKIDENENNGVLKKFCPHHLVNSESKAN
ITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGG
VVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGAT

--------------------------------------------------------------

>88320_88320_10_SWT1-DCAF8_SWT1_chr1_185159774_ENST00000367501_DCAF8_chr1_160195453_ENST00000608310_length(amino acids)=748AA_BP=506
MSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLYYNIKRRQGLKRLSVEIDTLRRRPKIG
SSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSPKIASDVKPKAEGQASENKWSHLLVQR
EKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTLQKLVEENVFNIDSNNSKTKQEEREYL
ESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSESIQDADQEMQIVEELHAARVGKSVDL
PGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPWVVMQELDRMKEGKLLKRAQHKAIPAV
HFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRKIDENENNGVLKKFCPHHLVNSESKAN
ITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSGSDCGHIFLWEKSSCQIIQFMEGDKGG
VVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSHMLWFLMHHLRQRRHHRRWREPGVGAT

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Fusion Protein Functional Features

Four levels of functional features of fusion genes
Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr1:185159774/chr1:160195453)
- FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels.
- How to search
1. Put your fusion gene symbol.
2. Press the tab key until there will be shown the breakpoint information filled.
4. Go down and press 'Search' tab twice.
4. Go down to have the hyperlink of the search result.
5. Click the hyperlink.
6. See the FGviewer result for your fusion gene.

Main function of each fusion partner protein. (from UniProt)

Hgene	Tgene
.	DCAF8 Q5TAQ9
FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.	FUNCTION: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. {ECO:0000269\|PubMed:16949367, ECO:0000269\|PubMed:16964240}.

Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

SWT1

chr1:185159774

chr1:160195453

ENST00000367500

38_44

507.6666666666667

901.0

Compositional bias

Note=Poly-Ser

Hgene

SWT1

chr1:185159774

chr1:160195453

ENST00000367501

38_44

507.6666666666667

901.0

Compositional bias

Note=Poly-Ser

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

385_424

356.6666666666667

598.0

Repeat

Note=WD 5

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

432_472

356.6666666666667

598.0

Repeat

Note=WD 6

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

476_515

356.6666666666667

598.0

Repeat

Note=WD 7

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

385_424

356.6666666666667

598.0

Repeat

Note=WD 5

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

432_472

356.6666666666667

598.0

Repeat

Note=WD 6

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

476_515

356.6666666666667

598.0

Repeat

Note=WD 7

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

385_424

356.6666666666667

598.0

Repeat

Note=WD 5

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

432_472

356.6666666666667

598.0

Repeat

Note=WD 6

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

476_515

356.6666666666667

598.0

Repeat

Note=WD 7

- Not-retained protein feature among the 13 regional features.

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Protein feature

Protein feature note

Hgene

SWT1

chr1:185159774

chr1:160195453

ENST00000367500

388_515

507.6666666666667

901.0

Domain

Note=PINc

Hgene

SWT1

chr1:185159774

chr1:160195453

ENST00000367501

388_515

507.6666666666667

901.0

Domain

Note=PINc

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

114_122

356.6666666666667

598.0

Motif

Nuclear localization signal

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

114_122

356.6666666666667

598.0

Motif

Nuclear localization signal

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

114_122

356.6666666666667

598.0

Motif

Nuclear localization signal

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

191_230

356.6666666666667

598.0

Repeat

Note=WD 1

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

234_275

356.6666666666667

598.0

Repeat

Note=WD 2

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

281_321

356.6666666666667

598.0

Repeat

Note=WD 3

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000326837

329_369

356.6666666666667

598.0

Repeat

Note=WD 4

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

191_230

356.6666666666667

598.0

Repeat

Note=WD 1

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

234_275

356.6666666666667

598.0

Repeat

Note=WD 2

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

281_321

356.6666666666667

598.0

Repeat

Note=WD 3

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368073

329_369

356.6666666666667

598.0

Repeat

Note=WD 4

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

191_230

356.6666666666667

598.0

Repeat

Note=WD 1

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

234_275

356.6666666666667

598.0

Repeat

Note=WD 2

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

281_321

356.6666666666667

598.0

Repeat

Note=WD 3

Tgene

DCAF8

chr1:185159774

chr1:160195453

ENST00000368074

329_369

356.6666666666667

598.0

Repeat

Note=WD 4

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Fusion Protein Structures

PDB and CIF files of the predicted fusion proteins
* Here we show the 3D structure of the fusion proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

Fusion protein PDB link (fusion AA seq ID in FusionPDB)	Hgene	Hchr	Hbp	Hstrand	Tgene	Tchr	Tbp	Tstrand	AA seq	Len(AA seq)
PDB file >>>1554_SWT1_185159774_DCAF8_160195453_ranked_0.pdb	SWT1	185159774	185159774	ENST00000556710	DCAF8	chr1	160195453	-	MGGGGPSPLAWGSGVATAAGAGKLFRMSSKESCGKKETSQRKDTTTSSPNFGEKDKKERKTPASSTSSSSIRSVSSEKRKLKSDHTDVLY YNIKRRQGLKRLSVEIDTLRRRPKIGSSSQRPIKLKEASYSNDNQIILQSPSSNGTKKDIHKCVDFKPKDIKLTNAGSKLDHGIKSLSSP KIASDVKPKAEGQASENKWSHLLVQREKMKELKKGRNSKFRDNSEKCVLEKWKRNQFSQDYNSNKIIKEPLGSRRQKISFKIPIKSRDTL QKLVEENVFNIDSNNSKTKQEEREYLESSQVSLNVTRQKTEHLLSDFTYKRTVHEWKRKHHYDHQESNDSHSRENLTQSFEAPCCSVSSE SIQDADQEMQIVEELHAARVGKSVDLPGELMSMEIDLEDDVHSSSANNTSDRKLLIVIDTNILMNHLKFVRILKTTEVPGFDKLVLIIPW VVMQELDRMKEGKLLKRAQHKAIPAVHFINDSLKNQDRKLWGQSIQLASQKHYGLSDENNDDRVLKCCLQHQELFPCSFVILCTIYDQRK IDENENNGVLKKFCPHHLVNSESKANITCLVYSHDGTELLASYNDEDIYLFNSSHSDGAQYVKRYKGHRNNATVKGVNFYGPKSEFVVSG SDCGHIFLWEKSSCQIIQFMEGDKGGVVNCLEPHPHLPVLATSGLDHDVKIWAPTAEASTELTGLKDVIKKNKRERDEDSLHQTDLFDSH	774

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pLDDT score distribution

pLDDT score distribution of the predicted wild-type structures of two partner proteins from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

SWT1_pLDDT.png

DCAF8_pLDDT.png

pLDDT score distribution of the predicted fusion protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

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Ramachandran Plot of Fusion Protein Structure

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this fusion protein peptide.

Fusion AA seq ID in FusionPDB and their Ramachandran plots

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Fusion Protein-Protein Interaction

Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in
ChiPPI page.

Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160)

Gene

PPI interactors

Protein-protein interactors based on sequence similarity (STRING)

Gene	STRING network
SWT1
DCAF8

- Retained interactions in fusion protein (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Still interaction with

- Lost interactions due to fusion (protein functional feature from UniProt).

Partner

Gene

Hbp

Tbp

ENST

Strand

BPexon

TotalExon

Protein feature loci

*BPloci

TotalLen

Interaction lost with

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Related Drugs to SWT1-DCAF8

Drugs used for this fusion-positive patient.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Drug

Source

PMID

Top

Related Diseases to SWT1-DCAF8

Diseases that have this fusion gene.
(Manual curation of PubMed, 04-30-2022 + MyCancerGenome)

Hgene

Tgene

Disease

Source

PMID

Diseases associated with fusion partners.
(DisGeNet 4.0)

Partner

Gene

Disease ID

Disease name

# pubmeds

Source