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Fusion Protein:TRIM24-ULK4 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: TRIM24-ULK4 | FusionPDB ID: 94012 | FusionGDB2.0 ID: 94012 | Hgene | Tgene | Gene symbol | TRIM24 | ULK4 | Gene ID | 8805 | 54986 |
| Gene name | tripartite motif containing 24 | unc-51 like kinase 4 | |
| Synonyms | PTC6|RNF82|TF1A|TIF1|TIF1A|TIF1ALPHA|hTIF1 | FAM7C1|REC01035 | |
| Cytomap | 7q33-q34 | 3p22.1 | |
| Type of gene | protein-coding | protein-coding | |
| Description | transcription intermediary factor 1-alphaE3 ubiquitin-protein ligase TRIM24RING finger protein 82RING-type E3 ubiquitin transferase TIF1-alphaTIF1-alphatranscriptional intermediary factor 1 | serine/threonine-protein kinase ULK4 | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | O15164 | . | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000497516, ENST00000343526, ENST00000415680, | ENST00000301831, ENST00000414606, ENST00000420927, ENST00000489118, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 10 X 9 X 7=630 | 14 X 21 X 5=1470 |
| # samples | 10 | 18 | |
| ** MAII score | log2(10/630*10)=-2.65535182861255 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(18/1470*10)=-3.02974734339405 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: TRIM24 [Title/Abstract] AND ULK4 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | TRIM24(138189153)-ULK4(41497086), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | TRIM24-ULK4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TRIM24-ULK4 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. TRIM24-ULK4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. TRIM24-ULK4 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | TRIM24 | GO:0016567 | protein ubiquitination | 19556538 |
| Hgene | TRIM24 | GO:0071391 | cellular response to estrogen stimulus | 21164480 |
| Fusion gene breakpoints across TRIM24 (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across ULK4 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | BRCA | TCGA-A8-A08R-01A | TRIM24 | chr7 | 138189153 | - | ULK4 | chr3 | 41497086 | - |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000343526 | TRIM24 | chr7 | 138189153 | - | ENST00000301831 | ULK4 | chr3 | 41497086 | - | 1455 | 698 | 92 | 1132 | 346 |
| ENST00000415680 | TRIM24 | chr7 | 138189153 | - | ENST00000301831 | ULK4 | chr3 | 41497086 | - | 1354 | 597 | 114 | 1031 | 305 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000343526 | ENST00000301831 | TRIM24 | chr7 | 138189153 | - | ULK4 | chr3 | 41497086 | - | 0.016810518 | 0.98318946 |
| ENST00000415680 | ENST00000301831 | TRIM24 | chr7 | 138189153 | - | ULK4 | chr3 | 41497086 | - | 0.016773792 | 0.98322624 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >94012_94012_1_TRIM24-ULK4_TRIM24_chr7_138189153_ENST00000343526_ULK4_chr3_41497086_ENST00000301831_length(amino acids)=346AA_BP=201 MALPRVHRAASEEQPQEEEEVVGGGGRRPRSRFPGGGKGRTMEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAAR LNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLMLPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHI IDNFFVKDTTEVPSSTVEKSNQAQKSGSGEDPQAAEDLLLLNRPLTDLISLLIPLLPNEDPEIFDVSSKCLSILVQLYGGENPDSLSPEN -------------------------------------------------------------- >94012_94012_2_TRIM24-ULK4_TRIM24_chr7_138189153_ENST00000415680_ULK4_chr3_41497086_ENST00000301831_length(amino acids)=305AA_BP=160 MEVAVEKAVAAAAAASAAASGGPSAAPSGENEAESRQGPDSERGGEAARLNLLDTCAVCHQNIQSRAPKLLPCLHSFCQRCLPAPQRYLM LPAPMLGSAETPPPVPAPGSPVSGSSPFATQVGVIRCPVCSQECAERHIIDNFFVKDTTEVPSSTVEKSNQAQKSGSGEDPQAAEDLLLL NRPLTDLISLLIPLLPNEDPEIFDVSSKCLSILVQLYGGENPDSLSPENVEIFAHLLTSKEDPKEQKLLLRILRRMITSNEKHLESLKNA -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chr7:138189153/chr3:41497086) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| TRIM24 | . |
| FUNCTION: Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity). {ECO:0000250, ECO:0000269|PubMed:16322096, ECO:0000269|PubMed:19556538, ECO:0000269|PubMed:21164480}. | FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 9_15 | 161.0 | 1051.0 | Compositional bias | Note=Poly-Ala |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 9_15 | 161.0 | 1017.0 | Compositional bias | Note=Poly-Ala |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 56_82 | 161.0 | 1051.0 | Zinc finger | RING-type |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 56_82 | 161.0 | 1017.0 | Zinc finger | RING-type |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 1147_1150 | 1131.0 | 1276.0 | Compositional bias | Note=Poly-Leu | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 1147_1150 | 0 | 581.0 | Compositional bias | Note=Poly-Leu | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 4_280 | 0 | 581.0 | Domain | Protein kinase | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 1151_1189 | 1131.0 | 1276.0 | Repeat | Note=HEAT 4 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 1213_1253 | 1131.0 | 1276.0 | Repeat | Note=HEAT 5 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 1025_1063 | 0 | 581.0 | Repeat | Note=HEAT 3 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 1151_1189 | 0 | 581.0 | Repeat | Note=HEAT 4 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 1213_1253 | 0 | 581.0 | Repeat | Note=HEAT 5 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 842_880 | 0 | 581.0 | Repeat | Note=HEAT 1 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000420927 | 0 | 18 | 926_964 | 0 | 581.0 | Repeat | Note=HEAT 2 |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 289_359 | 161.0 | 1051.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 289_359 | 161.0 | 1017.0 | Coiled coil | Ontology_term=ECO:0000255 |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 344_347 | 161.0 | 1051.0 | Compositional bias | Note=Poly-Gln |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 344_347 | 161.0 | 1017.0 | Compositional bias | Note=Poly-Gln |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 932_987 | 161.0 | 1051.0 | Domain | Bromo |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 932_987 | 161.0 | 1017.0 | Domain | Bromo |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 891_907 | 161.0 | 1051.0 | Motif | Nuclear localization signal |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 891_907 | 161.0 | 1017.0 | Motif | Nuclear localization signal |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 754_779 | 161.0 | 1051.0 | Region | Note=Nuclear receptor binding site (NRBS) |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 754_779 | 161.0 | 1017.0 | Region | Note=Nuclear receptor binding site (NRBS) |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 158_211 | 161.0 | 1051.0 | Zinc finger | B box-type 1 |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 218_259 | 161.0 | 1051.0 | Zinc finger | B box-type 2 |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 826_873 | 161.0 | 1051.0 | Zinc finger | PHD-type |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 158_211 | 161.0 | 1017.0 | Zinc finger | B box-type 1 |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 218_259 | 161.0 | 1017.0 | Zinc finger | B box-type 2 |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 826_873 | 161.0 | 1017.0 | Zinc finger | PHD-type |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 4_280 | 1131.0 | 1276.0 | Domain | Protein kinase | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 1025_1063 | 1131.0 | 1276.0 | Repeat | Note=HEAT 3 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 842_880 | 1131.0 | 1276.0 | Repeat | Note=HEAT 1 | |
| Tgene | ULK4 | chr7:138189153 | chr3:41497086 | ENST00000301831 | 32 | 37 | 926_964 | 1131.0 | 1276.0 | Repeat | Note=HEAT 2 |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| TRIM24 | ZNF10, TRIM28, TRIM24, PML, NR3C2, ESR1, THRA, RARA, RXRA, NR3C1, AR, TRIM33, GTF2E1, TAF7, TAF11, UBE2U, ATF4, NFE2L2, STAT6, FOSL1, ESR2, PPARG, TP53, BRD7, HIST1H3A, HIST1H3D, HIST1H3C, HIST1H3E, HIST1H3I, HIST1H3G, HIST1H3J, HIST1H3H, HIST1H3B, HIST1H3F, HIST2H4B, HIST1H4I, HIST1H4A, HIST1H4D, HIST1H4F, HIST1H4K, HIST1H4C, HIST1H4H, HIST1H4B, HIST1H4E, HIST1H4L, HIST2H4A, HIST1H4J, HIST4H4, NCOA1, NONO, CDK12, SNRPA, SF3B2, SF3B1, RNF141, RNF181, TRIM8, BRCA1, RNF2, MAGEA10, RFPL4B, QPRT, HSPB1, NTRK1, MUS81, PTEN, TCF4, FOXI1, USP37, NANOG, ZNF264, ZNF483, ZNF324B, WDYHV1, ZNF695, ZIM2, SYCE3, ZNF764, ZNF785, ZNF331, ZNF133, ZNF254, ZNF550, CDC16, CNKSR1, MTNR1B, DTNBP1, STAT3, NR1I3, SOX2, TNRC6A, UBE2I, MYC, MCM2, SUPT6H, MCM4, PEX5, MCM6, MCM7, SUPT16H, SSRP1, SPOP, FBXO38, PLEKHA4, CREBBP, SMG7, H2AFY, NPM1, H2AFY2, BRD2, SET, ZC3H18, RSF1, MBD1, E2F2, TOP2A, BRPF1, AFF4, PADI2, TFDP2, ZCCHC8, MLKL, PPIH, TFDP1, VPS72, ENPP6, GADD45GIP1, SEZ6L, RAD18, PSD, CDH16, YEATS4, DSC2, MLLT1, RNF43, C10orf71, RCOR2, TANC1, ACTR6, RIBC2, FMN2, RBM7, NOLC1, SRCAP, RNPS1, XPC, MFAP1, DNASE2B, XPO1, TRAF3, UBE2D1, DNAJC19, CEP78, CALM1, SUMO2, SOD1, MARCKS, CALR, PDIA4, DPP7, PTMA, RPS28, PDIA3, HSPE1, CSTB, ALDOA, MOB2, MIF, DDTL, RPRD1B, TPM3, CYCS, STMN1, BASP1, SSB, LCE3D, PGK1, PFN1, TKT, NPC2, PA2G4, TAB1, MARCKSL1, CNPY2, MYL12B, ARHGDIA, MTPN, TPI1, MDH1, PGAM1, TBCA, HMGN1, GGH, PTMS, HSP90B1, MAT2A, ANXA5, PPIB, FABP5, PDIA6, DDX39B, CMBL, TALDO1, TPM4, NUDC, CKB, NUCKS1, GSTO1, HDGF, TAGLN2, PRDX5, PARK7, PEBP1, MYL6, AHSG, SOD2, TRIM37, APEX1, ASF1A, CBX3, HIST1H2BG, TERF2IP, ZNF330, HORMAD2, MAD2L1, ZNF418, ZNF776, CRYAA, ZNF287, ABTB2, CCT8L2, ZNF17, ZNF718, ZNF484, KRT37, NUP62, PIAS4, KXD1, KNSTRN, MAGEA9, NUP62CL, ZNF726, LURAP1, ZNF383, KRT39, TGM7, ZNF460, ZNF543, KRT38, TRIM52, NLRP3, T, ELF1, ELF3, ELF4, ELF5, ERG, ETS1, ETV4, FEV, FOS, GATA2, HNF1B, HNF4A, KLF15, KLF5, KLF6, KLF8, LHX1, LHX2, LHX3, LHX4, MYOD1, NFIC, NFIX, PAX6, SOX17, SOX5, SP7, TLX3, YY1, |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| TRIM24 |  |
| ULK4 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000343526 | - | 2 | 19 | 834_840 | 161.0 | 1051.0 | histone H3 that is not methylated at 'Lys-4' (H3K4me0) |
| Hgene | TRIM24 | chr7:138189153 | chr3:41497086 | ENST00000415680 | - | 2 | 19 | 834_840 | 161.0 | 1017.0 | histone H3 that is not methylated at 'Lys-4' (H3K4me0) |
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Related Drugs to TRIM24-ULK4 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to TRIM24-ULK4 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
| Hgene | TRIM24 | C0238463 | Papillary thyroid carcinoma | 2 | ORPHANET |
| Hgene | TRIM24 | C0023893 | Liver Cirrhosis, Experimental | 1 | CTD_human |
| Hgene | TRIM24 | C2239176 | Liver carcinoma | 1 | CTD_human |
| Hgene | TRIM24 | C3658266 | Prostatic Cancer, Castration-Resistant | 1 | CTD_human |
| Hgene | TRIM24 | C3658267 | Prostatic Neoplasms, Castration-Resistant | 1 | CTD_human |
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