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Fusion Protein:USP9X-MAPKAPK5 |
Fusion Protein Summary |
 Fusion gene summary |
| Fusion partner gene information | Fusion gene name: USP9X-MAPKAPK5 | FusionPDB ID: 97659 | FusionGDB2.0 ID: 97659 | Hgene | Tgene | Gene symbol | USP9X | MAPKAPK5 | Gene ID | 8239 | 8550 |
| Gene name | ubiquitin specific peptidase 9 X-linked | MAPK activated protein kinase 5 | |
| Synonyms | DFFRX|FAF|FAM|MRX99|MRXS99F | MAPKAP-K5|MK-5|MK5|PRAK | |
| Cytomap | Xp11.4 | 12q24.12-q24.13 | |
| Type of gene | protein-coding | protein-coding | |
| Description | probable ubiquitin carboxyl-terminal hydrolase FAF-XDrosophila fat facets related, X-linkeddeubiquitinating enzyme FAF-Xfat facets in mammalsfat facets protein related, X-linkedhFAMubiquitin specific protease 9, X chromosome (fat facets-like Drosoph | MAP kinase-activated protein kinase 5MAPKAP kinase 5MAPKAPK-5mitogen-activated protein kinase-activated protein kinase 5p38-regulated/activated protein kinase | |
| Modification date | 20200313 | 20200313 | |
| UniProtAcc | . | Q8IW41 | |
| Ensembl transtripts involved in fusion gene | ENST ids | ENST00000324545, ENST00000378308, | ENST00000546394, ENST00000547305, ENST00000550735, ENST00000551404, |
| Fusion gene scores for assessment (based on all fusion genes of FusionGDB 2.0) | * DoF score | 19 X 12 X 12=2736 | 8 X 10 X 3=240 |
| # samples | 23 | 11 | |
| ** MAII score | log2(23/2736*10)=-3.57236246394152 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | log2(11/240*10)=-1.12553088208386 possibly effective Gene in Pan-Cancer Fusion Genes (peGinPCFGs). DoF>8 and MAII<0 | |
| Context (manual curation of fusion genes in FusionPDB) | PubMed: USP9X [Title/Abstract] AND MAPKAPK5 [Title/Abstract] AND fusion [Title/Abstract] | ||
| Most frequent breakpoint (based on all fusion genes of FusionGDB 2.0) | USP9X(40982977)-MAPKAPK5(112303054), # samples:1 | ||
| Anticipated loss of major functional domain due to fusion event. | USP9X-MAPKAPK5 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. USP9X-MAPKAPK5 seems lost the major protein functional domain in Hgene partner, which is a CGC by not retaining the major functional domain in the partially deleted in-frame ORF. USP9X-MAPKAPK5 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. USP9X-MAPKAPK5 seems lost the major protein functional domain in Hgene partner, which is a essential gene by not retaining the major functional domain in the partially deleted in-frame ORF. | ||
| * DoF score (Degree of Frequency) = # partners X # break points X # cancer types ** MAII score (Major Active Isofusion Index) = log2(# samples/DoF score*10) |
| Gene ontology of each fusion partner gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Hgene | USP9X | GO:0016579 | protein deubiquitination | 19135894 |
| Hgene | USP9X | GO:0030509 | BMP signaling pathway | 19135894 |
| Hgene | USP9X | GO:1901537 | positive regulation of DNA demethylation | 25944111 |
| Tgene | MAPKAPK5 | GO:0006417 | regulation of translation | 21329882 |
| Tgene | MAPKAPK5 | GO:0007265 | Ras protein signal transduction | 17254968 |
| Tgene | MAPKAPK5 | GO:0046777 | protein autophosphorylation | 21329882 |
| Tgene | MAPKAPK5 | GO:0090400 | stress-induced premature senescence | 17254968 |
| Fusion gene breakpoints across USP9X (5'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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| Fusion gene breakpoints across MAPKAPK5 (3'-gene) * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
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Fusion Gene Sample Information |
| Fusion gene information from FusionGDB2.0. |
| Fusion gene information from two resources (ChiTars 5.0 and ChimerDB 4.0) * All genome coordinats were lifted-over on hg19. * Click on the break point to see the gene structure around the break point region using the UCSC Genome Browser. |
| Source | Disease | Sample | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand |
| ChimerDB4 | PRAD | TCGA-HC-7818-01A | USP9X | chrX | 40982977 | - | MAPKAPK5 | chr12 | 112303054 | + |
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Fusion ORF Analysis |
| Fusion information from ORFfinder translation from full-length transcript sequence from FusionPDB. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | Seq length (transcript) | BP loci (transcript) | Predicted start (transcript) | Predicted stop (transcript) | Seq length (amino acids) |
| ENST00000378308 | USP9X | chrX | 40982977 | - | ENST00000550735 | MAPKAPK5 | chr12 | 112303054 | + | 2464 | 729 | 633 | 2108 | 491 |
| ENST00000378308 | USP9X | chrX | 40982977 | - | ENST00000551404 | MAPKAPK5 | chr12 | 112303054 | + | 2215 | 729 | 633 | 2114 | 493 |
| ENST00000324545 | USP9X | chrX | 40982977 | - | ENST00000550735 | MAPKAPK5 | chr12 | 112303054 | + | 2464 | 729 | 633 | 2108 | 491 |
| ENST00000324545 | USP9X | chrX | 40982977 | - | ENST00000551404 | MAPKAPK5 | chr12 | 112303054 | + | 2215 | 729 | 633 | 2114 | 493 |
| DeepORF prediction of the coding potential based on the fusion transcript sequence of in-frame fusion genes. DeepORF is a coding potential classifier based on convolutional neural network by comparing the real Ribo-seq data. If the no-coding score < 0.5 and coding score > 0.5, then the in-frame fusion transcript is predicted as being likely translated. |
| Henst | Tenst | Hgene | Hchr | Hbp | Hstrand | Tgene | Tchr | Tbp | Tstrand | No-coding score | Coding score |
| ENST00000378308 | ENST00000550735 | USP9X | chrX | 40982977 | - | MAPKAPK5 | chr12 | 112303054 | + | 0.002175834 | 0.9978242 |
| ENST00000378308 | ENST00000551404 | USP9X | chrX | 40982977 | - | MAPKAPK5 | chr12 | 112303054 | + | 0.002743075 | 0.99725693 |
| ENST00000324545 | ENST00000550735 | USP9X | chrX | 40982977 | - | MAPKAPK5 | chr12 | 112303054 | + | 0.002175834 | 0.9978242 |
| ENST00000324545 | ENST00000551404 | USP9X | chrX | 40982977 | - | MAPKAPK5 | chr12 | 112303054 | + | 0.002743075 | 0.99725693 |
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Fusion Amino Acid Sequences |
| For individual full-length fusion transcript sequence from FusionPDB, we ran ORFfinder and chose the longest ORF among the all predicted ones. |
| >FusionGDB ID_FusionGDB isoform ID_FGname_Hgene_Hchr_Hbp_Henst_Tgene_Tchr_Tbp_Tenst_length(fusion AA) seq_BP >97659_97659_1_USP9X-MAPKAPK5_USP9X_chrX_40982977_ENST00000324545_MAPKAPK5_chr12_112303054_ENST00000550735_length(amino acids)=491AA_BP=32 MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNS LDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTI PKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANM RIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLL -------------------------------------------------------------- >97659_97659_2_USP9X-MAPKAPK5_USP9X_chrX_40982977_ENST00000324545_MAPKAPK5_chr12_112303054_ENST00000551404_length(amino acids)=493AA_BP=32 MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNS LDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTI PKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANM RIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECK -------------------------------------------------------------- >97659_97659_3_USP9X-MAPKAPK5_USP9X_chrX_40982977_ENST00000378308_MAPKAPK5_chr12_112303054_ENST00000550735_length(amino acids)=491AA_BP=32 MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNS LDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTI PKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANM RIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGENEDEKLNEVMQEAWKYNRECKLL -------------------------------------------------------------- >97659_97659_4_USP9X-MAPKAPK5_USP9X_chrX_40982977_ENST00000378308_MAPKAPK5_chr12_112303054_ENST00000551404_length(amino acids)=493AA_BP=32 MTATTRGSPVGGNDNQGQAPDGQSQPPLQQNQETSILEEYSINWTQKLGAGISGPVRVCVKKSTQERFALKILLDRPKARNEVRLHMMCA THPNIVQIIEVFANSVQFPHESSPRARLLIVMEMMEGGELFHRISQHRHFTEKQASQVTKQIALALRHCHLLNIAHRDLKPENLLFKDNS LDAPVKLCDFGFAKIDQGDLMTPQFTPYYVAPQVLEAQRRHQKEKSGIIPTSPTPYTYNKSCDLWSLGVIIYVMLCGYPPFYSKHHSRTI PKDMRRKIMTGSFEFPEEEWSQISEMAKDVVRKLLKVKPEERLTIEGVLDHPWLNSTEALDNVLPSAQLMMDKAVVAGIQQAHAEQLANM RIQDLKVSLKPLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQCILPQAGKGENEDEKLNEVMQEAWKYNRECK -------------------------------------------------------------- |
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Fusion Protein Functional Features |
| Four levels of functional features of fusion genes Go to FGviewer search page for the most frequent breakpoint (https://ccsmweb.uth.edu/FGviewer/chrX:40982977/chr12:112303054) - FGviewer provides the online visualization of the retention search of the protein functional features across DNA, RNA, protein, and pathological levels. - How to search 1. Put your fusion gene symbol. 2. Press the tab key until there will be shown the breakpoint information filled. 4. Go down and press 'Search' tab twice. 4. Go down to have the hyperlink of the search result. 5. Click the hyperlink. 6. See the FGviewer result for your fusion gene. |
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| Main function of each fusion partner protein. (from UniProt) |
| Hgene | Tgene |
| . | MAPKAPK5 |
| FUNCTION: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. | FUNCTION: Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement. {ECO:0000269|PubMed:17254968, ECO:0000269|PubMed:17728103, ECO:0000269|PubMed:19166925, ECO:0000269|PubMed:21329882, ECO:0000269|PubMed:9628874}. |
| Retention analysis result of each fusion partner protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Tgene | MAPKAPK5 | chrX:40982977 | chr12:112303054 | ENST00000550735 | 0 | 14 | 409_440 | 12.0 | 472.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | MAPKAPK5 | chrX:40982977 | chr12:112303054 | ENST00000551404 | 0 | 14 | 409_440 | 12.0 | 474.0 | Coiled coil | Ontology_term=ECO:0000255 | |
| Tgene | MAPKAPK5 | chrX:40982977 | chr12:112303054 | ENST00000550735 | 0 | 14 | 22_304 | 12.0 | 472.0 | Domain | Protein kinase | |
| Tgene | MAPKAPK5 | chrX:40982977 | chr12:112303054 | ENST00000551404 | 0 | 14 | 22_304 | 12.0 | 474.0 | Domain | Protein kinase | |
| Tgene | MAPKAPK5 | chrX:40982977 | chr12:112303054 | ENST00000550735 | 0 | 14 | 28_36 | 12.0 | 472.0 | Nucleotide binding | ATP | |
| Tgene | MAPKAPK5 | chrX:40982977 | chr12:112303054 | ENST00000551404 | 0 | 14 | 28_36 | 12.0 | 474.0 | Nucleotide binding | ATP |
| - Not-retained protein feature among the 13 regional features. |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Protein feature | Protein feature note |
| Hgene | USP9X | chrX:40982977 | chr12:112303054 | ENST00000324545 | - | 2 | 45 | 1557_1956 | 32.0 | 2571.0 | Domain | USP |
| Hgene | USP9X | chrX:40982977 | chr12:112303054 | ENST00000378308 | - | 2 | 45 | 1557_1956 | 32.0 | 2555.0 | Domain | USP |
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Fusion Protein-Protein Interaction |
| Go to ChiPPI (Chimeric Protein-Protein interactions) to see the chimeric PPI interaction in |
| Protein-protein interactors with each fusion partner protein in wild-type from validated records (BIOGRID-3.4.160) |
| Gene | PPI interactors |
| Protein-protein interactors based on sequence similarity (STRING) |
| Gene | STRING network |
| USP9X | |
| MAPKAPK5 |
| - Retained interactions in fusion protein (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Still interaction with |
| - Lost interactions due to fusion (protein functional feature from UniProt). |
| Partner | Gene | Hbp | Tbp | ENST | Strand | BPexon | TotalExon | Protein feature loci | *BPloci | TotalLen | Interaction lost with |
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Related Drugs to USP9X-MAPKAPK5 |
| Drugs used for this fusion-positive patient. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Drug | Source | PMID |
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Related Diseases to USP9X-MAPKAPK5 |
| Diseases that have this fusion gene. (Manual curation of PubMed, 04-30-2022 + MyCancerGenome) |
| Hgene | Tgene | Disease | Source | PMID |
| Diseases associated with fusion partners. (DisGeNet 4.0) |
| Partner | Gene | Disease ID | Disease name | # pubmeds | Source |
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